Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus

Autores
Marani, Mariela Mirta; Dourado, Flávio Santos; Quelemes, Patrick Veras; Araujo, Alyne Rodrigues de; Perfeito, Márcia Luana Gomes; Barbosa, Eder Alves; Véras, Leiz Maria Costa; Coelho, Andreia Luísa Rodrigues; Andrade, Etielle Barroso; Eaton, Peter; Longo, João Paulo Figueiró; Azevedo, Ricardo Bentes; Matos, Cristina Delerue; Leite, José Roberto S. A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) have free carboxylates. Antimicrobial activity, hemolytic tests, and cytotoxicity against a murine fi broblast cell line were investigated. All peptides, except for ocellatin-PT2, have antimicrobial activity against at least one Gram-negative strain. Ocellatin-PT8 inhibited the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella choleraesuis strains with MICs in the 60 − 240 μ M range. No signi fi cant e ff ect was observed in human erythrocytes and in a murine fi broblast cell line after exposure to the peptides at MICs. A comparison between sequences obtained by both direct HPLC-MS de novo sequencing and cDNA cloning demonstrates the secretion of mature peptides derived from a pre-pro-peptide structure.
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Dourado, Flávio Santos. Secretaria de Vigilancia em Saude, Ministerio da Saude; Brasil
Fil: Quelemes, Patrick Veras. Universidade Federal do Piauí; Brasil
Fil: Araujo, Alyne Rodrigues de. Universidade Federal do Piauí; Brasil
Fil: Perfeito, Márcia Luana Gomes. Universidade Federal do Piauí; Brasil
Fil: Barbosa, Eder Alves. Universidade Do Brasilia; Brasil
Fil: Véras, Leiz Maria Costa. Universidade Federal do Piauí; Brasil
Fil: Coelho, Andreia Luísa Rodrigues. Universidade Federal do Piauí; Brasil. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Andrade, Etielle Barroso. Universidade Federal do Piauí; Brasil. Universidade Federal do Maranhaõ; Brasil
Fil: Eaton, Peter. Universidade do Porto; Portugal
Fil: Longo, João Paulo Figueiró. Universidade Do Brasilia; Brasil
Fil: Azevedo, Ricardo Bentes. Universidade Do Brasilia; Brasil
Fil: Matos, Cristina Delerue. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Leite, José Roberto S. A.. Universidade Federal do Piauí; Brasil
Materia
Peptidos
Antimicrobianos
Bioprospección
Anfibios
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19254
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19254
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatusMarani, Mariela MirtaDourado, Flávio SantosQuelemes, Patrick VerasAraujo, Alyne Rodrigues dePerfeito, Márcia Luana GomesBarbosa, Eder AlvesVéras, Leiz Maria CostaCoelho, Andreia Luísa RodriguesAndrade, Etielle BarrosoEaton, PeterLongo, João Paulo FigueiróAzevedo, Ricardo BentesMatos, Cristina DelerueLeite, José Roberto S. A.PeptidosAntimicrobianosBioprospecciónAnfibioshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) have free carboxylates. Antimicrobial activity, hemolytic tests, and cytotoxicity against a murine fi broblast cell line were investigated. All peptides, except for ocellatin-PT2, have antimicrobial activity against at least one Gram-negative strain. Ocellatin-PT8 inhibited the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella choleraesuis strains with MICs in the 60 − 240 μ M range. No signi fi cant e ff ect was observed in human erythrocytes and in a murine fi broblast cell line after exposure to the peptides at MICs. A comparison between sequences obtained by both direct HPLC-MS de novo sequencing and cDNA cloning demonstrates the secretion of mature peptides derived from a pre-pro-peptide structure.Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Dourado, Flávio Santos. Secretaria de Vigilancia em Saude, Ministerio da Saude; BrasilFil: Quelemes, Patrick Veras. Universidade Federal do Piauí; BrasilFil: Araujo, Alyne Rodrigues de. Universidade Federal do Piauí; BrasilFil: Perfeito, Márcia Luana Gomes. Universidade Federal do Piauí; BrasilFil: Barbosa, Eder Alves. Universidade Do Brasilia; BrasilFil: Véras, Leiz Maria Costa. Universidade Federal do Piauí; BrasilFil: Coelho, Andreia Luísa Rodrigues. Universidade Federal do Piauí; Brasil. Instituto Superior de Engenharia do Instituto Politécnico do Porto; PortugalFil: Andrade, Etielle Barroso. Universidade Federal do Piauí; Brasil. Universidade Federal do Maranhaõ; BrasilFil: Eaton, Peter. Universidade do Porto; PortugalFil: Longo, João Paulo Figueiró. Universidade Do Brasilia; BrasilFil: Azevedo, Ricardo Bentes. Universidade Do Brasilia; BrasilFil: Matos, Cristina Delerue. Instituto Superior de Engenharia do Instituto Politécnico do Porto; PortugalFil: Leite, José Roberto S. A.. Universidade Federal do Piauí; BrasilAmerican Chemical Society2015-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/19254Marani, Mariela Mirta; Dourado, Flávio Santos; Quelemes, Patrick Veras; Araujo, Alyne Rodrigues de; Perfeito, Márcia Luana Gomes; et al.; Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus; American Chemical Society; Journal Of Natural Products; 78; 7; 24-6-2015; 1495-15040163-3864enginfo:eu-repo/semantics/altIdentifier/doi/10.1021/np500907tinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/np500907tinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:48Zoai:ri.conicet.gov.ar:11336/19254instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:49.006CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
title Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
spellingShingle Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
Marani, Mariela Mirta
Peptidos
Antimicrobianos
Bioprospección
Anfibios
title_short Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
title_full Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
title_fullStr Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
title_full_unstemmed Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
title_sort Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus
dc.creator.none.fl_str_mv Marani, Mariela Mirta
Dourado, Flávio Santos
Quelemes, Patrick Veras
Araujo, Alyne Rodrigues de
Perfeito, Márcia Luana Gomes
Barbosa, Eder Alves
Véras, Leiz Maria Costa
Coelho, Andreia Luísa Rodrigues
Andrade, Etielle Barroso
Eaton, Peter
Longo, João Paulo Figueiró
Azevedo, Ricardo Bentes
Matos, Cristina Delerue
Leite, José Roberto S. A.
author Marani, Mariela Mirta
author_facet Marani, Mariela Mirta
Dourado, Flávio Santos
Quelemes, Patrick Veras
Araujo, Alyne Rodrigues de
Perfeito, Márcia Luana Gomes
Barbosa, Eder Alves
Véras, Leiz Maria Costa
Coelho, Andreia Luísa Rodrigues
Andrade, Etielle Barroso
Eaton, Peter
Longo, João Paulo Figueiró
Azevedo, Ricardo Bentes
Matos, Cristina Delerue
Leite, José Roberto S. A.
author_role author
author2 Dourado, Flávio Santos
Quelemes, Patrick Veras
Araujo, Alyne Rodrigues de
Perfeito, Márcia Luana Gomes
Barbosa, Eder Alves
Véras, Leiz Maria Costa
Coelho, Andreia Luísa Rodrigues
Andrade, Etielle Barroso
Eaton, Peter
Longo, João Paulo Figueiró
Azevedo, Ricardo Bentes
Matos, Cristina Delerue
Leite, José Roberto S. A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Peptidos
Antimicrobianos
Bioprospección
Anfibios
topic Peptidos
Antimicrobianos
Bioprospección
Anfibios
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) have free carboxylates. Antimicrobial activity, hemolytic tests, and cytotoxicity against a murine fi broblast cell line were investigated. All peptides, except for ocellatin-PT2, have antimicrobial activity against at least one Gram-negative strain. Ocellatin-PT8 inhibited the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella choleraesuis strains with MICs in the 60 − 240 μ M range. No signi fi cant e ff ect was observed in human erythrocytes and in a murine fi broblast cell line after exposure to the peptides at MICs. A comparison between sequences obtained by both direct HPLC-MS de novo sequencing and cDNA cloning demonstrates the secretion of mature peptides derived from a pre-pro-peptide structure.
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Dourado, Flávio Santos. Secretaria de Vigilancia em Saude, Ministerio da Saude; Brasil
Fil: Quelemes, Patrick Veras. Universidade Federal do Piauí; Brasil
Fil: Araujo, Alyne Rodrigues de. Universidade Federal do Piauí; Brasil
Fil: Perfeito, Márcia Luana Gomes. Universidade Federal do Piauí; Brasil
Fil: Barbosa, Eder Alves. Universidade Do Brasilia; Brasil
Fil: Véras, Leiz Maria Costa. Universidade Federal do Piauí; Brasil
Fil: Coelho, Andreia Luísa Rodrigues. Universidade Federal do Piauí; Brasil. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Andrade, Etielle Barroso. Universidade Federal do Piauí; Brasil. Universidade Federal do Maranhaõ; Brasil
Fil: Eaton, Peter. Universidade do Porto; Portugal
Fil: Longo, João Paulo Figueiró. Universidade Do Brasilia; Brasil
Fil: Azevedo, Ricardo Bentes. Universidade Do Brasilia; Brasil
Fil: Matos, Cristina Delerue. Instituto Superior de Engenharia do Instituto Politécnico do Porto; Portugal
Fil: Leite, José Roberto S. A.. Universidade Federal do Piauí; Brasil
description Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) have free carboxylates. Antimicrobial activity, hemolytic tests, and cytotoxicity against a murine fi broblast cell line were investigated. All peptides, except for ocellatin-PT2, have antimicrobial activity against at least one Gram-negative strain. Ocellatin-PT8 inhibited the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella choleraesuis strains with MICs in the 60 − 240 μ M range. No signi fi cant e ff ect was observed in human erythrocytes and in a murine fi broblast cell line after exposure to the peptides at MICs. A comparison between sequences obtained by both direct HPLC-MS de novo sequencing and cDNA cloning demonstrates the secretion of mature peptides derived from a pre-pro-peptide structure.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19254
Marani, Mariela Mirta; Dourado, Flávio Santos; Quelemes, Patrick Veras; Araujo, Alyne Rodrigues de; Perfeito, Márcia Luana Gomes; et al.; Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus; American Chemical Society; Journal Of Natural Products; 78; 7; 24-6-2015; 1495-1504
0163-3864
url http://hdl.handle.net/11336/19254
identifier_str_mv Marani, Mariela Mirta; Dourado, Flávio Santos; Quelemes, Patrick Veras; Araujo, Alyne Rodrigues de; Perfeito, Márcia Luana Gomes; et al.; Characterization and biological activities of ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus; American Chemical Society; Journal Of Natural Products; 78; 7; 24-6-2015; 1495-1504
0163-3864
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/np500907t
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/np500907t
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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