Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex

Autores
Roggero Luque, Juan Manuel; Moreno, Ercilia Maria Sara; Kovalsky, Ivana Evelìn; Seijo, Jose Guillermo; Solis Neffa, Viviana Griselda
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Turnera sidoides L. (x = 7) is an autopolyploid complex of obligate outcrossing perennial herbs. Includes five taxonomic subspecies and seven morphotypes in which different ploidy levels were found from diploid to octoploid. The particular karyotypical features of each subspecies remain independently from the ploidy level. In order to infer the chromosomal differentiation processes that occurred during the diversification of T. sidoides, chromosome complements of representative diploid populations of subspecies carnea, holosericea and four morphotypes of the subspecies pinnatifida were analysed in this work by chromosome banding using chromomycine A3 (CMA) and 4?,6-diaminio-2-phenylindole (DAPI) fluorochromes and by fluorescence in situ hybridization (FISH) with 45S and 5S rDNA probes. All morphotypes and subspecies analyzed showed CMA+/DAPI- bands associated to secondary constrictions. DAPI staining was uniform in all the chromosomes of the complements and no DAPI+ bands were detected. On the other hand, FISH experiments showed that, in all subspecies and morphotypes, the 45S ribosomal DNA loci were always consistent with the localization of CMA+/DAPI- bands associated with the secondary constrictions. The pattern of CMA/DAPI bands and 45S and 5S rDNA loci varied among the three subspecies analyzed. On the other hand, the pattern of FISH signals was conserved in the four analyzed morphotypes of the subspecies pinantifida. The results obtained in this work show that at least part of the chromosomal differentiation of the T. sidoides complex would have occurred at diploid level.
Fil: Roggero Luque, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Moreno, Ercilia Maria Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Kovalsky, Ivana Evelìn. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Solis Neffa, Viviana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Materia
Turnera Sidoides
Fish
Chromosome Differentiation
Diploid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9312
Acceder
id CONICETDig_d5235ac877932e1c98d5a72a63f1f032
oai_identifier_str oai:ri.conicet.gov.ar:11336/9312
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complexRoggero Luque, Juan ManuelMoreno, Ercilia Maria SaraKovalsky, Ivana EvelìnSeijo, Jose GuillermoSolis Neffa, Viviana GriseldaTurnera SidoidesFishChromosome DifferentiationDiploidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Turnera sidoides L. (x = 7) is an autopolyploid complex of obligate outcrossing perennial herbs. Includes five taxonomic subspecies and seven morphotypes in which different ploidy levels were found from diploid to octoploid. The particular karyotypical features of each subspecies remain independently from the ploidy level. In order to infer the chromosomal differentiation processes that occurred during the diversification of T. sidoides, chromosome complements of representative diploid populations of subspecies carnea, holosericea and four morphotypes of the subspecies pinnatifida were analysed in this work by chromosome banding using chromomycine A3 (CMA) and 4?,6-diaminio-2-phenylindole (DAPI) fluorochromes and by fluorescence in situ hybridization (FISH) with 45S and 5S rDNA probes. All morphotypes and subspecies analyzed showed CMA+/DAPI- bands associated to secondary constrictions. DAPI staining was uniform in all the chromosomes of the complements and no DAPI+ bands were detected. On the other hand, FISH experiments showed that, in all subspecies and morphotypes, the 45S ribosomal DNA loci were always consistent with the localization of CMA+/DAPI- bands associated with the secondary constrictions. The pattern of CMA/DAPI bands and 45S and 5S rDNA loci varied among the three subspecies analyzed. On the other hand, the pattern of FISH signals was conserved in the four analyzed morphotypes of the subspecies pinantifida. The results obtained in this work show that at least part of the chromosomal differentiation of the T. sidoides complex would have occurred at diploid level.Fil: Roggero Luque, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Moreno, Ercilia Maria Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Kovalsky, Ivana Evelìn. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Solis Neffa, Viviana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaNatl Research Council Canada-n R C Research Press2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/9312Roggero Luque, Juan Manuel; Moreno, Ercilia Maria Sara; Kovalsky, Ivana Evelìn; Seijo, Jose Guillermo; Solis Neffa, Viviana Griselda; Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex; Natl Research Council Canada-n R C Research Press; Genome; 59; 2; 11-2015; 127-1360831-2796enginfo:eu-repo/semantics/altIdentifier/doi/10.1139/gen-2015-0096info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:03:50Zoai:ri.conicet.gov.ar:11336/9312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:03:50.728CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
title Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
spellingShingle Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
Roggero Luque, Juan Manuel
Turnera Sidoides
Fish
Chromosome Differentiation
Diploid
title_short Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
title_full Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
title_fullStr Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
title_full_unstemmed Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
title_sort Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex
dc.creator.none.fl_str_mv Roggero Luque, Juan Manuel
Moreno, Ercilia Maria Sara
Kovalsky, Ivana Evelìn
Seijo, Jose Guillermo
Solis Neffa, Viviana Griselda
author Roggero Luque, Juan Manuel
author_facet Roggero Luque, Juan Manuel
Moreno, Ercilia Maria Sara
Kovalsky, Ivana Evelìn
Seijo, Jose Guillermo
Solis Neffa, Viviana Griselda
author_role author
author2 Moreno, Ercilia Maria Sara
Kovalsky, Ivana Evelìn
Seijo, Jose Guillermo
Solis Neffa, Viviana Griselda
author2_role author
author
author
author
dc.subject.none.fl_str_mv Turnera Sidoides
Fish
Chromosome Differentiation
Diploid
topic Turnera Sidoides
Fish
Chromosome Differentiation
Diploid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Turnera sidoides L. (x = 7) is an autopolyploid complex of obligate outcrossing perennial herbs. Includes five taxonomic subspecies and seven morphotypes in which different ploidy levels were found from diploid to octoploid. The particular karyotypical features of each subspecies remain independently from the ploidy level. In order to infer the chromosomal differentiation processes that occurred during the diversification of T. sidoides, chromosome complements of representative diploid populations of subspecies carnea, holosericea and four morphotypes of the subspecies pinnatifida were analysed in this work by chromosome banding using chromomycine A3 (CMA) and 4?,6-diaminio-2-phenylindole (DAPI) fluorochromes and by fluorescence in situ hybridization (FISH) with 45S and 5S rDNA probes. All morphotypes and subspecies analyzed showed CMA+/DAPI- bands associated to secondary constrictions. DAPI staining was uniform in all the chromosomes of the complements and no DAPI+ bands were detected. On the other hand, FISH experiments showed that, in all subspecies and morphotypes, the 45S ribosomal DNA loci were always consistent with the localization of CMA+/DAPI- bands associated with the secondary constrictions. The pattern of CMA/DAPI bands and 45S and 5S rDNA loci varied among the three subspecies analyzed. On the other hand, the pattern of FISH signals was conserved in the four analyzed morphotypes of the subspecies pinantifida. The results obtained in this work show that at least part of the chromosomal differentiation of the T. sidoides complex would have occurred at diploid level.
Fil: Roggero Luque, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Moreno, Ercilia Maria Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Kovalsky, Ivana Evelìn. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Solis Neffa, Viviana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
description Turnera sidoides L. (x = 7) is an autopolyploid complex of obligate outcrossing perennial herbs. Includes five taxonomic subspecies and seven morphotypes in which different ploidy levels were found from diploid to octoploid. The particular karyotypical features of each subspecies remain independently from the ploidy level. In order to infer the chromosomal differentiation processes that occurred during the diversification of T. sidoides, chromosome complements of representative diploid populations of subspecies carnea, holosericea and four morphotypes of the subspecies pinnatifida were analysed in this work by chromosome banding using chromomycine A3 (CMA) and 4?,6-diaminio-2-phenylindole (DAPI) fluorochromes and by fluorescence in situ hybridization (FISH) with 45S and 5S rDNA probes. All morphotypes and subspecies analyzed showed CMA+/DAPI- bands associated to secondary constrictions. DAPI staining was uniform in all the chromosomes of the complements and no DAPI+ bands were detected. On the other hand, FISH experiments showed that, in all subspecies and morphotypes, the 45S ribosomal DNA loci were always consistent with the localization of CMA+/DAPI- bands associated with the secondary constrictions. The pattern of CMA/DAPI bands and 45S and 5S rDNA loci varied among the three subspecies analyzed. On the other hand, the pattern of FISH signals was conserved in the four analyzed morphotypes of the subspecies pinantifida. The results obtained in this work show that at least part of the chromosomal differentiation of the T. sidoides complex would have occurred at diploid level.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9312
Roggero Luque, Juan Manuel; Moreno, Ercilia Maria Sara; Kovalsky, Ivana Evelìn; Seijo, Jose Guillermo; Solis Neffa, Viviana Griselda; Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex; Natl Research Council Canada-n R C Research Press; Genome; 59; 2; 11-2015; 127-136
0831-2796
url http://hdl.handle.net/11336/9312
identifier_str_mv Roggero Luque, Juan Manuel; Moreno, Ercilia Maria Sara; Kovalsky, Ivana Evelìn; Seijo, Jose Guillermo; Solis Neffa, Viviana Griselda; Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid Turnera sidoides L. (Passifloraceae) complex; Natl Research Council Canada-n R C Research Press; Genome; 59; 2; 11-2015; 127-136
0831-2796
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/gen-2015-0096
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980111184297984
score 12.993085

Publicaciones similares