Chromosomal aberrations induced by Mitomycin C in canine lymphocytes

Autores
Caliri Barchiesi, Martina Noel; Ferré, Daniela Marisol; Ledda, Valeria; Gorla, Nora Bibiana Maria
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chromosomal aberrations comprise an abnormal number of chromosomes as well as changes in the structure of the chromosomes. Aberrations in the structure of chromosomes, such as gaps and breaks in chromatids and chromosomes, acentric fragments, telomeric associations, deletions, early chromatid-separation and large scale effects, such as pulverized metaphases, and sticky metaphases. In this study, the effect of Mitomycin C, a recognized clastogen on human lymphocytes, was assayed in cultures of canine lymphocytes, an animal species that is flourishing in comparative medicine. Blood cultures were performed on samples from a male and a female dog. Cytotoxicity using the mitotic index and genotoxicity testing were performed with 0.25 µg/ml Mitomycin C. The total chromosomal aberrations were significantly higher due to the effect of Mitomycin C (P=0.0247). The number of chromatid breaks nearly quintupled, while pulverized metaphases were found to be six times more frequent, and endoduplicated cells were three times higher than in negative control cultures. The quantitatively most relevant chromosomal aberration was the presence of sticky metaphases, related to adverse effects in chromatin proteins. The potential use of canine lymphocytes for chromosomal aberration assay is emphasized in the evaluation or re-evaluation of the genotoxic in vitro effect of xenobiotics, to evidence chromosomal damage.
Fil: Caliri Barchiesi, Martina Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; Argentina
Fil: Ferré, Daniela Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; Argentina
Fil: Ledda, Valeria. Universidad del Aconcagua; Argentina
Fil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; Argentina
Materia
CANINE CELLS
CHROMOSOMAL DAMAGE
CLASTOGENIC AGENTS
CYTOGENETICS
LYMPHOCYTE CULTURES
MITOMYCIN C
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/223034
Acceder
id CONICETDig_d4f3bdb116407ede57752ffed70ae683
oai_identifier_str oai:ri.conicet.gov.ar:11336/223034
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chromosomal aberrations induced by Mitomycin C in canine lymphocytesKromosomske aberacije uzrokovane mitomicinom C u psećim limfocitimaCaliri Barchiesi, Martina NoelFerré, Daniela MarisolLedda, ValeriaGorla, Nora Bibiana MariaCANINE CELLSCHROMOSOMAL DAMAGECLASTOGENIC AGENTSCYTOGENETICSLYMPHOCYTE CULTURESMITOMYCIN Chttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chromosomal aberrations comprise an abnormal number of chromosomes as well as changes in the structure of the chromosomes. Aberrations in the structure of chromosomes, such as gaps and breaks in chromatids and chromosomes, acentric fragments, telomeric associations, deletions, early chromatid-separation and large scale effects, such as pulverized metaphases, and sticky metaphases. In this study, the effect of Mitomycin C, a recognized clastogen on human lymphocytes, was assayed in cultures of canine lymphocytes, an animal species that is flourishing in comparative medicine. Blood cultures were performed on samples from a male and a female dog. Cytotoxicity using the mitotic index and genotoxicity testing were performed with 0.25 µg/ml Mitomycin C. The total chromosomal aberrations were significantly higher due to the effect of Mitomycin C (P=0.0247). The number of chromatid breaks nearly quintupled, while pulverized metaphases were found to be six times more frequent, and endoduplicated cells were three times higher than in negative control cultures. The quantitatively most relevant chromosomal aberration was the presence of sticky metaphases, related to adverse effects in chromatin proteins. The potential use of canine lymphocytes for chromosomal aberration assay is emphasized in the evaluation or re-evaluation of the genotoxic in vitro effect of xenobiotics, to evidence chromosomal damage.Fil: Caliri Barchiesi, Martina Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; ArgentinaFil: Ferré, Daniela Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; ArgentinaFil: Ledda, Valeria. Universidad del Aconcagua; ArgentinaFil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; ArgentinaUniv Zagreb Vet Faculty2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223034Caliri Barchiesi, Martina Noel; Ferré, Daniela Marisol; Ledda, Valeria; Gorla, Nora Bibiana Maria; Chromosomal aberrations induced by Mitomycin C in canine lymphocytes ; Univ Zagreb Vet Faculty; Veterinarski Arhiv; 93; 3; 8-2023; 341-3500372-5480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://hrcak.srce.hr/en/307410info:eu-repo/semantics/altIdentifier/doi/10.24099/vet.arhiv.1698info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:54Zoai:ri.conicet.gov.ar:11336/223034instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:55.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
Kromosomske aberacije uzrokovane mitomicinom C u psećim limfocitima
title Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
spellingShingle Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
Caliri Barchiesi, Martina Noel
CANINE CELLS
CHROMOSOMAL DAMAGE
CLASTOGENIC AGENTS
CYTOGENETICS
LYMPHOCYTE CULTURES
MITOMYCIN C
title_short Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
title_full Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
title_fullStr Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
title_full_unstemmed Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
title_sort Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
dc.creator.none.fl_str_mv Caliri Barchiesi, Martina Noel
Ferré, Daniela Marisol
Ledda, Valeria
Gorla, Nora Bibiana Maria
author Caliri Barchiesi, Martina Noel
author_facet Caliri Barchiesi, Martina Noel
Ferré, Daniela Marisol
Ledda, Valeria
Gorla, Nora Bibiana Maria
author_role author
author2 Ferré, Daniela Marisol
Ledda, Valeria
Gorla, Nora Bibiana Maria
author2_role author
author
author
dc.subject.none.fl_str_mv CANINE CELLS
CHROMOSOMAL DAMAGE
CLASTOGENIC AGENTS
CYTOGENETICS
LYMPHOCYTE CULTURES
MITOMYCIN C
topic CANINE CELLS
CHROMOSOMAL DAMAGE
CLASTOGENIC AGENTS
CYTOGENETICS
LYMPHOCYTE CULTURES
MITOMYCIN C
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chromosomal aberrations comprise an abnormal number of chromosomes as well as changes in the structure of the chromosomes. Aberrations in the structure of chromosomes, such as gaps and breaks in chromatids and chromosomes, acentric fragments, telomeric associations, deletions, early chromatid-separation and large scale effects, such as pulverized metaphases, and sticky metaphases. In this study, the effect of Mitomycin C, a recognized clastogen on human lymphocytes, was assayed in cultures of canine lymphocytes, an animal species that is flourishing in comparative medicine. Blood cultures were performed on samples from a male and a female dog. Cytotoxicity using the mitotic index and genotoxicity testing were performed with 0.25 µg/ml Mitomycin C. The total chromosomal aberrations were significantly higher due to the effect of Mitomycin C (P=0.0247). The number of chromatid breaks nearly quintupled, while pulverized metaphases were found to be six times more frequent, and endoduplicated cells were three times higher than in negative control cultures. The quantitatively most relevant chromosomal aberration was the presence of sticky metaphases, related to adverse effects in chromatin proteins. The potential use of canine lymphocytes for chromosomal aberration assay is emphasized in the evaluation or re-evaluation of the genotoxic in vitro effect of xenobiotics, to evidence chromosomal damage.
Fil: Caliri Barchiesi, Martina Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; Argentina
Fil: Ferré, Daniela Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; Argentina
Fil: Ledda, Valeria. Universidad del Aconcagua; Argentina
Fil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad "Juan Agustín Maza"; Argentina
description Chromosomal aberrations comprise an abnormal number of chromosomes as well as changes in the structure of the chromosomes. Aberrations in the structure of chromosomes, such as gaps and breaks in chromatids and chromosomes, acentric fragments, telomeric associations, deletions, early chromatid-separation and large scale effects, such as pulverized metaphases, and sticky metaphases. In this study, the effect of Mitomycin C, a recognized clastogen on human lymphocytes, was assayed in cultures of canine lymphocytes, an animal species that is flourishing in comparative medicine. Blood cultures were performed on samples from a male and a female dog. Cytotoxicity using the mitotic index and genotoxicity testing were performed with 0.25 µg/ml Mitomycin C. The total chromosomal aberrations were significantly higher due to the effect of Mitomycin C (P=0.0247). The number of chromatid breaks nearly quintupled, while pulverized metaphases were found to be six times more frequent, and endoduplicated cells were three times higher than in negative control cultures. The quantitatively most relevant chromosomal aberration was the presence of sticky metaphases, related to adverse effects in chromatin proteins. The potential use of canine lymphocytes for chromosomal aberration assay is emphasized in the evaluation or re-evaluation of the genotoxic in vitro effect of xenobiotics, to evidence chromosomal damage.
publishDate 2023
dc.date.none.fl_str_mv 2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223034
Caliri Barchiesi, Martina Noel; Ferré, Daniela Marisol; Ledda, Valeria; Gorla, Nora Bibiana Maria; Chromosomal aberrations induced by Mitomycin C in canine lymphocytes ; Univ Zagreb Vet Faculty; Veterinarski Arhiv; 93; 3; 8-2023; 341-350
0372-5480
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223034
identifier_str_mv Caliri Barchiesi, Martina Noel; Ferré, Daniela Marisol; Ledda, Valeria; Gorla, Nora Bibiana Maria; Chromosomal aberrations induced by Mitomycin C in canine lymphocytes ; Univ Zagreb Vet Faculty; Veterinarski Arhiv; 93; 3; 8-2023; 341-350
0372-5480
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://hrcak.srce.hr/en/307410
info:eu-repo/semantics/altIdentifier/doi/10.24099/vet.arhiv.1698
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Zagreb Vet Faculty
publisher.none.fl_str_mv Univ Zagreb Vet Faculty
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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