The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos

Autores
Cardozo Gizzi, Andres Mauricio; Prucca, Cesar German; Gaveglio, Virginia Lucía; Renner, Marianne L.; Pasquaré, Susana J.; Caputto, Beatriz Leonor
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphatidic acid (PA) is a central precursor for membrane phospholipid biosynthesis. Lipin family is a Mg-dependent type I PA phosphatase,involved in de novo synthesis of neutral lipids and of phospholipids. The regulation of Lipin activity may govern the pathways by which these lipids aresynthesized and control the cellular levels ofimportant signaling lipids. On the other hand, the proto-oncoprotein c-Fos has an emerging role in glycerolipid synthesis regulation: by interacting with key synthetizing enzymes it is able toincrease overall phopho- and glyco- lipid synthesis.We studied the Lipin 1β enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it in the presence/absence of c-Fos. We found that Lipin 1β kcat increases around 40% in the presence of c- Fos, with no change in the Lipin 1β affinity for the PA/Triton X-100 mixed micelles. We also probed a physical interaction between both proteins. While the c-Fos domain involved in Lipin activation is its basic domain (BD), the interaction domain is mapped to the c-Fos N-terminal. In conclusion, we provide evidence for a novel positive regulator of Lipin 1β PA phosphatase activity that is not achieved via altering its subcellular localization or affinity for membranesbut rather through directly increasing its catalytic efficiency.
Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Fil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Materia
LIPIN
C-FOS
PHOSPHATIDIC ACID
PHOSPHATIDIC ACID PHOSPHATASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7601
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7601
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-FosCardozo Gizzi, Andres MauricioPrucca, Cesar GermanGaveglio, Virginia LucíaRenner, Marianne L.Pasquaré, Susana J.Caputto, Beatriz LeonorLIPINC-FOSPHOSPHATIDIC ACIDPHOSPHATIDIC ACID PHOSPHATASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phosphatidic acid (PA) is a central precursor for membrane phospholipid biosynthesis. Lipin family is a Mg-dependent type I PA phosphatase,involved in de novo synthesis of neutral lipids and of phospholipids. The regulation of Lipin activity may govern the pathways by which these lipids aresynthesized and control the cellular levels ofimportant signaling lipids. On the other hand, the proto-oncoprotein c-Fos has an emerging role in glycerolipid synthesis regulation: by interacting with key synthetizing enzymes it is able toincrease overall phopho- and glyco- lipid synthesis.We studied the Lipin 1β enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it in the presence/absence of c-Fos. We found that Lipin 1β kcat increases around 40% in the presence of c- Fos, with no change in the Lipin 1β affinity for the PA/Triton X-100 mixed micelles. We also probed a physical interaction between both proteins. While the c-Fos domain involved in Lipin activation is its basic domain (BD), the interaction domain is mapped to the c-Fos N-terminal. In conclusion, we provide evidence for a novel positive regulator of Lipin 1β PA phosphatase activity that is not achieved via altering its subcellular localization or affinity for membranesbut rather through directly increasing its catalytic efficiency.Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); ArgentinaFil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaAmerican Society For Biochemistry And Molecular Biology2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7601Cardozo Gizzi, Andres Mauricio; Prucca, Cesar German; Gaveglio, Virginia Lucía; Renner, Marianne L.; Pasquaré, Susana J.; et al.; The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 290; 10-2015; 29578-295920021-9258enginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/290/49/29578info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M115.678821info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:18Zoai:ri.conicet.gov.ar:11336/7601instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:18.532CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
title The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
spellingShingle The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
Cardozo Gizzi, Andres Mauricio
LIPIN
C-FOS
PHOSPHATIDIC ACID
PHOSPHATIDIC ACID PHOSPHATASE
title_short The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
title_full The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
title_fullStr The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
title_full_unstemmed The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
title_sort The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
dc.creator.none.fl_str_mv Cardozo Gizzi, Andres Mauricio
Prucca, Cesar German
Gaveglio, Virginia Lucía
Renner, Marianne L.
Pasquaré, Susana J.
Caputto, Beatriz Leonor
author Cardozo Gizzi, Andres Mauricio
author_facet Cardozo Gizzi, Andres Mauricio
Prucca, Cesar German
Gaveglio, Virginia Lucía
Renner, Marianne L.
Pasquaré, Susana J.
Caputto, Beatriz Leonor
author_role author
author2 Prucca, Cesar German
Gaveglio, Virginia Lucía
Renner, Marianne L.
Pasquaré, Susana J.
Caputto, Beatriz Leonor
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LIPIN
C-FOS
PHOSPHATIDIC ACID
PHOSPHATIDIC ACID PHOSPHATASE
topic LIPIN
C-FOS
PHOSPHATIDIC ACID
PHOSPHATIDIC ACID PHOSPHATASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phosphatidic acid (PA) is a central precursor for membrane phospholipid biosynthesis. Lipin family is a Mg-dependent type I PA phosphatase,involved in de novo synthesis of neutral lipids and of phospholipids. The regulation of Lipin activity may govern the pathways by which these lipids aresynthesized and control the cellular levels ofimportant signaling lipids. On the other hand, the proto-oncoprotein c-Fos has an emerging role in glycerolipid synthesis regulation: by interacting with key synthetizing enzymes it is able toincrease overall phopho- and glyco- lipid synthesis.We studied the Lipin 1β enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it in the presence/absence of c-Fos. We found that Lipin 1β kcat increases around 40% in the presence of c- Fos, with no change in the Lipin 1β affinity for the PA/Triton X-100 mixed micelles. We also probed a physical interaction between both proteins. While the c-Fos domain involved in Lipin activation is its basic domain (BD), the interaction domain is mapped to the c-Fos N-terminal. In conclusion, we provide evidence for a novel positive regulator of Lipin 1β PA phosphatase activity that is not achieved via altering its subcellular localization or affinity for membranesbut rather through directly increasing its catalytic efficiency.
Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
Fil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
description Phosphatidic acid (PA) is a central precursor for membrane phospholipid biosynthesis. Lipin family is a Mg-dependent type I PA phosphatase,involved in de novo synthesis of neutral lipids and of phospholipids. The regulation of Lipin activity may govern the pathways by which these lipids aresynthesized and control the cellular levels ofimportant signaling lipids. On the other hand, the proto-oncoprotein c-Fos has an emerging role in glycerolipid synthesis regulation: by interacting with key synthetizing enzymes it is able toincrease overall phopho- and glyco- lipid synthesis.We studied the Lipin 1β enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it in the presence/absence of c-Fos. We found that Lipin 1β kcat increases around 40% in the presence of c- Fos, with no change in the Lipin 1β affinity for the PA/Triton X-100 mixed micelles. We also probed a physical interaction between both proteins. While the c-Fos domain involved in Lipin activation is its basic domain (BD), the interaction domain is mapped to the c-Fos N-terminal. In conclusion, we provide evidence for a novel positive regulator of Lipin 1β PA phosphatase activity that is not achieved via altering its subcellular localization or affinity for membranesbut rather through directly increasing its catalytic efficiency.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7601
Cardozo Gizzi, Andres Mauricio; Prucca, Cesar German; Gaveglio, Virginia Lucía; Renner, Marianne L.; Pasquaré, Susana J.; et al.; The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 290; 10-2015; 29578-29592
0021-9258
url http://hdl.handle.net/11336/7601
identifier_str_mv Cardozo Gizzi, Andres Mauricio; Prucca, Cesar German; Gaveglio, Virginia Lucía; Renner, Marianne L.; Pasquaré, Susana J.; et al.; The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 290; 10-2015; 29578-29592
0021-9258
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/290/49/29578
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M115.678821
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Biochemistry And Molecular Biology
publisher.none.fl_str_mv American Society For Biochemistry And Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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