Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain
- Autores
- Gruget, Clémence; Bello, Oscar Daniel; Coleman, Jeff; Krishnakumar, Shyam S.; Perez, Eric; Rothman, James E.; Pincet, Frederic; Donaldson, Stephen H.
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Synaptotagmin interaction with anionic lipid (phosphatidylserine/phosphatidylinositol) containing membranes, both in the absence and presence of calcium ions (Ca2+), is critical to its central role in orchestrating neurotransmitter release. The molecular surfaces involved, namely the conserved polylysine motif in the C2B domain and Ca2+-binding aliphatic loops on both C2A and C2B domains, are known. Here we use surface force apparatus combined with systematic mutational analysis of the functional surfaces to directly measure Syt1-membrane interaction and fully map the site-binding energetics of Syt1 both in the absence and presence of Ca2+. By correlating energetics data with the molecular rearrangements measured during confinement, we find that both C2 domains cooperate in membrane binding, with the C2B domain functioning as the main energetic driver, and the C2A domain acting as a facilitator.
Fil: Gruget, Clémence. Ecole Normale Supérieure; Francia
Fil: Bello, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Coleman, Jeff. University of Yale. School of Medicine; Estados Unidos
Fil: Krishnakumar, Shyam S.. University of Yale. School of Medicine; Estados Unidos
Fil: Perez, Eric. Ecole Normale Supérieure; Francia
Fil: Rothman, James E.. University of Yale. School of Medicine; Estados Unidos
Fil: Pincet, Frederic. Ecole Normale Supérieure; Francia. University of Yale. School of Medicine; Estados Unidos
Fil: Donaldson, Stephen H.. Ecole Normale Supérieure; Francia - Materia
-
EXOCYTOSIS
MOLECULAR
CONFORMATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/141422
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Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domainGruget, ClémenceBello, Oscar DanielColeman, JeffKrishnakumar, Shyam S.Perez, EricRothman, James E.Pincet, FredericDonaldson, Stephen H.EXOCYTOSISMOLECULARCONFORMATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Synaptotagmin interaction with anionic lipid (phosphatidylserine/phosphatidylinositol) containing membranes, both in the absence and presence of calcium ions (Ca2+), is critical to its central role in orchestrating neurotransmitter release. The molecular surfaces involved, namely the conserved polylysine motif in the C2B domain and Ca2+-binding aliphatic loops on both C2A and C2B domains, are known. Here we use surface force apparatus combined with systematic mutational analysis of the functional surfaces to directly measure Syt1-membrane interaction and fully map the site-binding energetics of Syt1 both in the absence and presence of Ca2+. By correlating energetics data with the molecular rearrangements measured during confinement, we find that both C2 domains cooperate in membrane binding, with the C2B domain functioning as the main energetic driver, and the C2A domain acting as a facilitator.Fil: Gruget, Clémence. Ecole Normale Supérieure; FranciaFil: Bello, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Coleman, Jeff. University of Yale. School of Medicine; Estados UnidosFil: Krishnakumar, Shyam S.. University of Yale. School of Medicine; Estados UnidosFil: Perez, Eric. Ecole Normale Supérieure; FranciaFil: Rothman, James E.. University of Yale. School of Medicine; Estados UnidosFil: Pincet, Frederic. Ecole Normale Supérieure; Francia. University of Yale. School of Medicine; Estados UnidosFil: Donaldson, Stephen H.. Ecole Normale Supérieure; FranciaNature2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141422Gruget, Clémence; Bello, Oscar Daniel; Coleman, Jeff; Krishnakumar, Shyam S.; Perez, Eric; et al.; Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain; Nature; Scientific Reports; 10; 1; 10-2020; 1-102045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-74923-yinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-74923-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:26Zoai:ri.conicet.gov.ar:11336/141422instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:26.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
title |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
spellingShingle |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain Gruget, Clémence EXOCYTOSIS MOLECULAR CONFORMATION |
title_short |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
title_full |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
title_fullStr |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
title_full_unstemmed |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
title_sort |
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain |
dc.creator.none.fl_str_mv |
Gruget, Clémence Bello, Oscar Daniel Coleman, Jeff Krishnakumar, Shyam S. Perez, Eric Rothman, James E. Pincet, Frederic Donaldson, Stephen H. |
author |
Gruget, Clémence |
author_facet |
Gruget, Clémence Bello, Oscar Daniel Coleman, Jeff Krishnakumar, Shyam S. Perez, Eric Rothman, James E. Pincet, Frederic Donaldson, Stephen H. |
author_role |
author |
author2 |
Bello, Oscar Daniel Coleman, Jeff Krishnakumar, Shyam S. Perez, Eric Rothman, James E. Pincet, Frederic Donaldson, Stephen H. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
EXOCYTOSIS MOLECULAR CONFORMATION |
topic |
EXOCYTOSIS MOLECULAR CONFORMATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Synaptotagmin interaction with anionic lipid (phosphatidylserine/phosphatidylinositol) containing membranes, both in the absence and presence of calcium ions (Ca2+), is critical to its central role in orchestrating neurotransmitter release. The molecular surfaces involved, namely the conserved polylysine motif in the C2B domain and Ca2+-binding aliphatic loops on both C2A and C2B domains, are known. Here we use surface force apparatus combined with systematic mutational analysis of the functional surfaces to directly measure Syt1-membrane interaction and fully map the site-binding energetics of Syt1 both in the absence and presence of Ca2+. By correlating energetics data with the molecular rearrangements measured during confinement, we find that both C2 domains cooperate in membrane binding, with the C2B domain functioning as the main energetic driver, and the C2A domain acting as a facilitator. Fil: Gruget, Clémence. Ecole Normale Supérieure; Francia Fil: Bello, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Coleman, Jeff. University of Yale. School of Medicine; Estados Unidos Fil: Krishnakumar, Shyam S.. University of Yale. School of Medicine; Estados Unidos Fil: Perez, Eric. Ecole Normale Supérieure; Francia Fil: Rothman, James E.. University of Yale. School of Medicine; Estados Unidos Fil: Pincet, Frederic. Ecole Normale Supérieure; Francia. University of Yale. School of Medicine; Estados Unidos Fil: Donaldson, Stephen H.. Ecole Normale Supérieure; Francia |
description |
Synaptotagmin interaction with anionic lipid (phosphatidylserine/phosphatidylinositol) containing membranes, both in the absence and presence of calcium ions (Ca2+), is critical to its central role in orchestrating neurotransmitter release. The molecular surfaces involved, namely the conserved polylysine motif in the C2B domain and Ca2+-binding aliphatic loops on both C2A and C2B domains, are known. Here we use surface force apparatus combined with systematic mutational analysis of the functional surfaces to directly measure Syt1-membrane interaction and fully map the site-binding energetics of Syt1 both in the absence and presence of Ca2+. By correlating energetics data with the molecular rearrangements measured during confinement, we find that both C2 domains cooperate in membrane binding, with the C2B domain functioning as the main energetic driver, and the C2A domain acting as a facilitator. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/141422 Gruget, Clémence; Bello, Oscar Daniel; Coleman, Jeff; Krishnakumar, Shyam S.; Perez, Eric; et al.; Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain; Nature; Scientific Reports; 10; 1; 10-2020; 1-10 2045-2322 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/141422 |
identifier_str_mv |
Gruget, Clémence; Bello, Oscar Daniel; Coleman, Jeff; Krishnakumar, Shyam S.; Perez, Eric; et al.; Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain; Nature; Scientific Reports; 10; 1; 10-2020; 1-10 2045-2322 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-74923-y info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-74923-y |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nature |
publisher.none.fl_str_mv |
Nature |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614090577674240 |
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13.070432 |