NLRP3 inflammasome activation is required for fibrosis development in NAFLD
- Autores
- Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; Inzaugarat, Maria Eugenia; Messer, Karen; Canbay, Ali; Hoffman, Hal M.; Feldstein, Ariel E.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease.
Fil: Wree, Alexander. University of California at San Diego; Estados Unidos. University Hospital Essen; Alemania
Fil: McGeough, Matthew D.. University of California at San Diego; Estados Unidos
Fil: Peña, Carla A.. University of California at San Diego; Estados Unidos
Fil: Schlattjan, Martin. University Hospital Essen; Alemania
Fil: Li, Hongying. University of California at San Diego; Estados Unidos
Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Messer, Karen. University of California at San Diego; Estados Unidos
Fil: Canbay, Ali. University Hospital Essen; Alemania
Fil: Hoffman, Hal M.. University of California at San Diego; Estados Unidos. Ludwig Institute of Cancer Research; Estados Unidos
Fil: Feldstein, Ariel E.. University of California at San Diego; Estados Unidos - Materia
-
NLRP3
Inflammation
Liver fibrosis
NASH
Steatoheptatisis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/18640
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NLRP3 inflammasome activation is required for fibrosis development in NAFLDWree, AlexanderMcGeough, Matthew D.Peña, Carla A.Schlattjan, MartinLi, HongyingInzaugarat, Maria EugeniaMesser, KarenCanbay, AliHoffman, Hal M.Feldstein, Ariel E.NLRP3InflammationLiver fibrosisNASHSteatoheptatisishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease.Fil: Wree, Alexander. University of California at San Diego; Estados Unidos. University Hospital Essen; AlemaniaFil: McGeough, Matthew D.. University of California at San Diego; Estados UnidosFil: Peña, Carla A.. University of California at San Diego; Estados UnidosFil: Schlattjan, Martin. University Hospital Essen; AlemaniaFil: Li, Hongying. University of California at San Diego; Estados UnidosFil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Messer, Karen. University of California at San Diego; Estados UnidosFil: Canbay, Ali. University Hospital Essen; AlemaniaFil: Hoffman, Hal M.. University of California at San Diego; Estados Unidos. Ludwig Institute of Cancer Research; Estados UnidosFil: Feldstein, Ariel E.. University of California at San Diego; Estados UnidosSpringer Verlag Berlín2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18640Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; et al.; NLRP3 inflammasome activation is required for fibrosis development in NAFLD; Springer Verlag Berlín; Journal of Molecular Medicine (Berlin, Germany); 92; 10; 10-2014; 1069-10820946-27161432-1440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00109-014-1170-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00109-014-1170-1info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349416/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:26Zoai:ri.conicet.gov.ar:11336/18640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:26.287CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
title |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
spellingShingle |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD Wree, Alexander NLRP3 Inflammation Liver fibrosis NASH Steatoheptatisis |
title_short |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
title_full |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
title_fullStr |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
title_full_unstemmed |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
title_sort |
NLRP3 inflammasome activation is required for fibrosis development in NAFLD |
dc.creator.none.fl_str_mv |
Wree, Alexander McGeough, Matthew D. Peña, Carla A. Schlattjan, Martin Li, Hongying Inzaugarat, Maria Eugenia Messer, Karen Canbay, Ali Hoffman, Hal M. Feldstein, Ariel E. |
author |
Wree, Alexander |
author_facet |
Wree, Alexander McGeough, Matthew D. Peña, Carla A. Schlattjan, Martin Li, Hongying Inzaugarat, Maria Eugenia Messer, Karen Canbay, Ali Hoffman, Hal M. Feldstein, Ariel E. |
author_role |
author |
author2 |
McGeough, Matthew D. Peña, Carla A. Schlattjan, Martin Li, Hongying Inzaugarat, Maria Eugenia Messer, Karen Canbay, Ali Hoffman, Hal M. Feldstein, Ariel E. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
NLRP3 Inflammation Liver fibrosis NASH Steatoheptatisis |
topic |
NLRP3 Inflammation Liver fibrosis NASH Steatoheptatisis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease. Fil: Wree, Alexander. University of California at San Diego; Estados Unidos. University Hospital Essen; Alemania Fil: McGeough, Matthew D.. University of California at San Diego; Estados Unidos Fil: Peña, Carla A.. University of California at San Diego; Estados Unidos Fil: Schlattjan, Martin. University Hospital Essen; Alemania Fil: Li, Hongying. University of California at San Diego; Estados Unidos Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Messer, Karen. University of California at San Diego; Estados Unidos Fil: Canbay, Ali. University Hospital Essen; Alemania Fil: Hoffman, Hal M.. University of California at San Diego; Estados Unidos. Ludwig Institute of Cancer Research; Estados Unidos Fil: Feldstein, Ariel E.. University of California at San Diego; Estados Unidos |
description |
NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/18640 Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; et al.; NLRP3 inflammasome activation is required for fibrosis development in NAFLD; Springer Verlag Berlín; Journal of Molecular Medicine (Berlin, Germany); 92; 10; 10-2014; 1069-1082 0946-2716 1432-1440 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/18640 |
identifier_str_mv |
Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; et al.; NLRP3 inflammasome activation is required for fibrosis development in NAFLD; Springer Verlag Berlín; Journal of Molecular Medicine (Berlin, Germany); 92; 10; 10-2014; 1069-1082 0946-2716 1432-1440 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00109-014-1170-1 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00109-014-1170-1 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349416/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag Berlín |
publisher.none.fl_str_mv |
Springer Verlag Berlín |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |