NLRP3 inflammasome activation is required for fibrosis development in NAFLD

Autores
Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; Inzaugarat, Maria Eugenia; Messer, Karen; Canbay, Ali; Hoffman, Hal M.; Feldstein, Ariel E.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease.
Fil: Wree, Alexander. University of California at San Diego; Estados Unidos. University Hospital Essen; Alemania
Fil: McGeough, Matthew D.. University of California at San Diego; Estados Unidos
Fil: Peña, Carla A.. University of California at San Diego; Estados Unidos
Fil: Schlattjan, Martin. University Hospital Essen; Alemania
Fil: Li, Hongying. University of California at San Diego; Estados Unidos
Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Messer, Karen. University of California at San Diego; Estados Unidos
Fil: Canbay, Ali. University Hospital Essen; Alemania
Fil: Hoffman, Hal M.. University of California at San Diego; Estados Unidos. Ludwig Institute of Cancer Research; Estados Unidos
Fil: Feldstein, Ariel E.. University of California at San Diego; Estados Unidos
Materia
NLRP3
Inflammation
Liver fibrosis
NASH
Steatoheptatisis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18640

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network_name_str CONICET Digital (CONICET)
spelling NLRP3 inflammasome activation is required for fibrosis development in NAFLDWree, AlexanderMcGeough, Matthew D.Peña, Carla A.Schlattjan, MartinLi, HongyingInzaugarat, Maria EugeniaMesser, KarenCanbay, AliHoffman, Hal M.Feldstein, Ariel E.NLRP3InflammationLiver fibrosisNASHSteatoheptatisishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease.Fil: Wree, Alexander. University of California at San Diego; Estados Unidos. University Hospital Essen; AlemaniaFil: McGeough, Matthew D.. University of California at San Diego; Estados UnidosFil: Peña, Carla A.. University of California at San Diego; Estados UnidosFil: Schlattjan, Martin. University Hospital Essen; AlemaniaFil: Li, Hongying. University of California at San Diego; Estados UnidosFil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Messer, Karen. University of California at San Diego; Estados UnidosFil: Canbay, Ali. University Hospital Essen; AlemaniaFil: Hoffman, Hal M.. University of California at San Diego; Estados Unidos. Ludwig Institute of Cancer Research; Estados UnidosFil: Feldstein, Ariel E.. University of California at San Diego; Estados UnidosSpringer Verlag Berlín2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18640Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; et al.; NLRP3 inflammasome activation is required for fibrosis development in NAFLD; Springer Verlag Berlín; Journal of Molecular Medicine (Berlin, Germany); 92; 10; 10-2014; 1069-10820946-27161432-1440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00109-014-1170-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00109-014-1170-1info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349416/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:26Zoai:ri.conicet.gov.ar:11336/18640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:26.287CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NLRP3 inflammasome activation is required for fibrosis development in NAFLD
title NLRP3 inflammasome activation is required for fibrosis development in NAFLD
spellingShingle NLRP3 inflammasome activation is required for fibrosis development in NAFLD
Wree, Alexander
NLRP3
Inflammation
Liver fibrosis
NASH
Steatoheptatisis
title_short NLRP3 inflammasome activation is required for fibrosis development in NAFLD
title_full NLRP3 inflammasome activation is required for fibrosis development in NAFLD
title_fullStr NLRP3 inflammasome activation is required for fibrosis development in NAFLD
title_full_unstemmed NLRP3 inflammasome activation is required for fibrosis development in NAFLD
title_sort NLRP3 inflammasome activation is required for fibrosis development in NAFLD
dc.creator.none.fl_str_mv Wree, Alexander
McGeough, Matthew D.
Peña, Carla A.
Schlattjan, Martin
Li, Hongying
Inzaugarat, Maria Eugenia
Messer, Karen
Canbay, Ali
Hoffman, Hal M.
Feldstein, Ariel E.
author Wree, Alexander
author_facet Wree, Alexander
McGeough, Matthew D.
Peña, Carla A.
Schlattjan, Martin
Li, Hongying
Inzaugarat, Maria Eugenia
Messer, Karen
Canbay, Ali
Hoffman, Hal M.
Feldstein, Ariel E.
author_role author
author2 McGeough, Matthew D.
Peña, Carla A.
Schlattjan, Martin
Li, Hongying
Inzaugarat, Maria Eugenia
Messer, Karen
Canbay, Ali
Hoffman, Hal M.
Feldstein, Ariel E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NLRP3
Inflammation
Liver fibrosis
NASH
Steatoheptatisis
topic NLRP3
Inflammation
Liver fibrosis
NASH
Steatoheptatisis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease.
Fil: Wree, Alexander. University of California at San Diego; Estados Unidos. University Hospital Essen; Alemania
Fil: McGeough, Matthew D.. University of California at San Diego; Estados Unidos
Fil: Peña, Carla A.. University of California at San Diego; Estados Unidos
Fil: Schlattjan, Martin. University Hospital Essen; Alemania
Fil: Li, Hongying. University of California at San Diego; Estados Unidos
Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Messer, Karen. University of California at San Diego; Estados Unidos
Fil: Canbay, Ali. University Hospital Essen; Alemania
Fil: Hoffman, Hal M.. University of California at San Diego; Estados Unidos. Ludwig Institute of Cancer Research; Estados Unidos
Fil: Feldstein, Ariel E.. University of California at San Diego; Estados Unidos
description NLR inflammasomes, caspase 1 activation platforms critical for processing key pro-inflammatory cytokines, have been implicated in the development of nonalcoholic fatty liver disease (NAFLD). As the direct role of the NLRP3 inflammasome remains unclear, we tested effects of persistent NLRP3 activation as a contributor to NAFLD development and, in particular, as a modulator of progression from benign hepatic steatosis to steatohepatitis during diet-induced NAFLD. Gain of function tamoxifen-inducible Nlrp3 knock-in mice allowing for in vivo temporal control of NLRP3 activation and loss of function Nlrp3 knockout mice were placed on short-term choline-deficient amino acid-defined (CDAA) diet, to induce isolated hepatic steatosis or long-term CDAA exposure, to induce severe steatohepatitis and fibrosis, respectively. Expression of NLRP3 associated proteins was assessed in liver biopsies of a well-characterized group of patients with the full spectrum of NAFLD. Nlrp3−/− mice were protected from long-term feeding CDAA-induced hepatomegaly, liver injury, and infiltration of activated macrophages. More importantly, Nlrp3−/− mice showed marked protection from CDAA-induced liver fibrosis. After 4 weeks on CDAA diet, wild-type (WT) animals showed isolated hepatic steatosis while Nlrp3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages and early signs of liver fibrosis. In the liver samples of patients with NAFLD, inflammasome components were significantly increased in those patients with nonalcoholic steatohepatitis (NASH) when compared to those with non-NASH NAFLD with mRNA levels of pro-IL1 beta correlated to levels of COL1A1. Our study uncovers a crucial role for the NLRP3 inflammasome in the development of NAFLD. These findings may lead to novel therapeutic strategies aimed at halting the progression of hepatic steatosis to the more severe forms of this disease.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18640
Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; et al.; NLRP3 inflammasome activation is required for fibrosis development in NAFLD; Springer Verlag Berlín; Journal of Molecular Medicine (Berlin, Germany); 92; 10; 10-2014; 1069-1082
0946-2716
1432-1440
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18640
identifier_str_mv Wree, Alexander; McGeough, Matthew D.; Peña, Carla A.; Schlattjan, Martin; Li, Hongying; et al.; NLRP3 inflammasome activation is required for fibrosis development in NAFLD; Springer Verlag Berlín; Journal of Molecular Medicine (Berlin, Germany); 92; 10; 10-2014; 1069-1082
0946-2716
1432-1440
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00109-014-1170-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00109-014-1170-1
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349416/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag Berlín
publisher.none.fl_str_mv Springer Verlag Berlín
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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