Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression

Autores
Abba, Martín Carlos; Canzoneri, Romina; Gurruchaga, Agustina; Lee, Jaeho; Tatineni, Pradeep; Kil, Hyunsuk; Lacunza, Ezequiel; Aldaz, Claudio Marcelo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer models. We determined that LINC00885 induces premalignant phenotypic changes by increasing cell proliferation, motility, migration and altering 3D growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by LINC00885, which include bioprocesses related to TP53 signaling pathway and proliferative signatures such as activation of EREG, EGFR and FOXM1 pathways. LINC00885 silencing in breast cancer lines overexpressing this lncRNA leads to downregulation of proliferation related transcripts such as EREG, CMYC, CCND1 and to significant decrease in cell migration and motility. TCGA-BRCA data analyses show an association between high LINC00885 expression and worse overall survival in patients with primary invasive breast carcinomas (p = 0.024), suggesting that the pro-tumorigenic effects of LINC00885 overexpression persist post-invasion. We conclude that LINC00885 behaves as a positive regulator of cell growth both in normal and DCIS breast cells possibly operating as a ceRNA and representing a novel oncogenic lncRNA associated with early stage breast cancer progression.
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Canzoneri, Romina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gurruchaga, Agustina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Lee, Jaeho. University of Texas; Estados Unidos
Fil: Tatineni, Pradeep. University of Texas; Estados Unidos
Fil: Kil, Hyunsuk. University of Texas; Estados Unidos
Fil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Aldaz, Claudio Marcelo. University of Texas; Estados Unidos
Materia
BREAST CANCER
DCIS
INVASION
LINC00885
LNCRNA
PROLIFERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144762
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/144762
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progressionAbba, Martín CarlosCanzoneri, RominaGurruchaga, AgustinaLee, JaehoTatineni, PradeepKil, HyunsukLacunza, EzequielAldaz, Claudio MarceloBREAST CANCERDCISINVASIONLINC00885LNCRNAPROLIFERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer models. We determined that LINC00885 induces premalignant phenotypic changes by increasing cell proliferation, motility, migration and altering 3D growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by LINC00885, which include bioprocesses related to TP53 signaling pathway and proliferative signatures such as activation of EREG, EGFR and FOXM1 pathways. LINC00885 silencing in breast cancer lines overexpressing this lncRNA leads to downregulation of proliferation related transcripts such as EREG, CMYC, CCND1 and to significant decrease in cell migration and motility. TCGA-BRCA data analyses show an association between high LINC00885 expression and worse overall survival in patients with primary invasive breast carcinomas (p = 0.024), suggesting that the pro-tumorigenic effects of LINC00885 overexpression persist post-invasion. We conclude that LINC00885 behaves as a positive regulator of cell growth both in normal and DCIS breast cells possibly operating as a ceRNA and representing a novel oncogenic lncRNA associated with early stage breast cancer progression.Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Canzoneri, Romina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gurruchaga, Agustina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Lee, Jaeho. University of Texas; Estados UnidosFil: Tatineni, Pradeep. University of Texas; Estados UnidosFil: Kil, Hyunsuk. University of Texas; Estados UnidosFil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Aldaz, Claudio Marcelo. University of Texas; Estados UnidosMDPI AG2020-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144762Abba, Martín Carlos; Canzoneri, Romina; Gurruchaga, Agustina; Lee, Jaeho; Tatineni, Pradeep; et al.; Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression; MDPI AG; International Journal of Molecular Sciences; 21; 19; 08-10-2020; 1-151422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/21/19/7407info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms21197407info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:04Zoai:ri.conicet.gov.ar:11336/144762instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:05.21CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
title Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
spellingShingle Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
Abba, Martín Carlos
BREAST CANCER
DCIS
INVASION
LINC00885
LNCRNA
PROLIFERATION
title_short Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
title_full Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
title_fullStr Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
title_full_unstemmed Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
title_sort Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression
dc.creator.none.fl_str_mv Abba, Martín Carlos
Canzoneri, Romina
Gurruchaga, Agustina
Lee, Jaeho
Tatineni, Pradeep
Kil, Hyunsuk
Lacunza, Ezequiel
Aldaz, Claudio Marcelo
author Abba, Martín Carlos
author_facet Abba, Martín Carlos
Canzoneri, Romina
Gurruchaga, Agustina
Lee, Jaeho
Tatineni, Pradeep
Kil, Hyunsuk
Lacunza, Ezequiel
Aldaz, Claudio Marcelo
author_role author
author2 Canzoneri, Romina
Gurruchaga, Agustina
Lee, Jaeho
Tatineni, Pradeep
Kil, Hyunsuk
Lacunza, Ezequiel
Aldaz, Claudio Marcelo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
DCIS
INVASION
LINC00885
LNCRNA
PROLIFERATION
topic BREAST CANCER
DCIS
INVASION
LINC00885
LNCRNA
PROLIFERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer models. We determined that LINC00885 induces premalignant phenotypic changes by increasing cell proliferation, motility, migration and altering 3D growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by LINC00885, which include bioprocesses related to TP53 signaling pathway and proliferative signatures such as activation of EREG, EGFR and FOXM1 pathways. LINC00885 silencing in breast cancer lines overexpressing this lncRNA leads to downregulation of proliferation related transcripts such as EREG, CMYC, CCND1 and to significant decrease in cell migration and motility. TCGA-BRCA data analyses show an association between high LINC00885 expression and worse overall survival in patients with primary invasive breast carcinomas (p = 0.024), suggesting that the pro-tumorigenic effects of LINC00885 overexpression persist post-invasion. We conclude that LINC00885 behaves as a positive regulator of cell growth both in normal and DCIS breast cells possibly operating as a ceRNA and representing a novel oncogenic lncRNA associated with early stage breast cancer progression.
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Canzoneri, Romina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gurruchaga, Agustina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Lee, Jaeho. University of Texas; Estados Unidos
Fil: Tatineni, Pradeep. University of Texas; Estados Unidos
Fil: Kil, Hyunsuk. University of Texas; Estados Unidos
Fil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Aldaz, Claudio Marcelo. University of Texas; Estados Unidos
description Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer models. We determined that LINC00885 induces premalignant phenotypic changes by increasing cell proliferation, motility, migration and altering 3D growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by LINC00885, which include bioprocesses related to TP53 signaling pathway and proliferative signatures such as activation of EREG, EGFR and FOXM1 pathways. LINC00885 silencing in breast cancer lines overexpressing this lncRNA leads to downregulation of proliferation related transcripts such as EREG, CMYC, CCND1 and to significant decrease in cell migration and motility. TCGA-BRCA data analyses show an association between high LINC00885 expression and worse overall survival in patients with primary invasive breast carcinomas (p = 0.024), suggesting that the pro-tumorigenic effects of LINC00885 overexpression persist post-invasion. We conclude that LINC00885 behaves as a positive regulator of cell growth both in normal and DCIS breast cells possibly operating as a ceRNA and representing a novel oncogenic lncRNA associated with early stage breast cancer progression.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144762
Abba, Martín Carlos; Canzoneri, Romina; Gurruchaga, Agustina; Lee, Jaeho; Tatineni, Pradeep; et al.; Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression; MDPI AG; International Journal of Molecular Sciences; 21; 19; 08-10-2020; 1-15
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144762
identifier_str_mv Abba, Martín Carlos; Canzoneri, Romina; Gurruchaga, Agustina; Lee, Jaeho; Tatineni, Pradeep; et al.; Linc00885 a novel oncogenic long non-coding rna associated with early stage breast cancer progression; MDPI AG; International Journal of Molecular Sciences; 21; 19; 08-10-2020; 1-15
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/21/19/7407
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms21197407
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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