Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index

Autores
Matturri, Luigi; Milei, Jose; Grana, Daniel Rodolfo; Lavezzi, Anna Maria
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: At present little is known about the biological basis of cellular alterations in myocardial hypertrophy. The present study aims to analyze proliferating cell nuclear antigen (PCNA) expression, DNA content and apoptosis, in several types of myocardial hypertrophy in order to define the biological characteristics of this process. Methods: The biological parameters were investigated in normal hearts (n=4) and in 21 cases of left ventricular myocardial hypertrophy related to pressure overload (n=7), post-infarction remodeling (n=8) and hypertrophic cardiomyopathy (HCM) (n=8). Results: The analyzed biomarkers were similar in hypertension and in remodeling, with a very high apoptotic index (mean values: 8.1 and 8.5%, respectively), a low PCNA positivity (mean values: 1.8 and 1.6%) and a prevalent diploid DNA content (DNA index: 1.2). Conversely, HCM showed a high mean PCNA index (21.2%) associated with a prevalence of hyperdiploid myocytes (DNA index: 1.8) and a low number of apoptotic cells (mean value: 1.7%). Conclusions: There are significant biological differences between hypertrophy in HCM and that related to arterial hypertension and post-infarction remodeling. Therefore, the combined evaluation of DNA content, PCNA and apoptotic indices could provide a powerful diagnostic tool in doubtful cases of myocardial primary or secondary hypertrophy and open new avenues in the clinical treatment of these entities.
Fil: Matturri, Luigi. Università degli Studi di Milano; Italia
Fil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador; Argentina
Fil: Lavezzi, Anna Maria. Università degli Studi di Milano; Italia
Materia
APOPTOSIS
DNA
MYOCARDIAL HYPERTROPHY
PROLIFERATING CELL NUCLEAR ANTIGEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181923
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic indexMatturri, LuigiMilei, JoseGrana, Daniel RodolfoLavezzi, Anna MariaAPOPTOSISDNAMYOCARDIAL HYPERTROPHYPROLIFERATING CELL NUCLEAR ANTIGENhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background: At present little is known about the biological basis of cellular alterations in myocardial hypertrophy. The present study aims to analyze proliferating cell nuclear antigen (PCNA) expression, DNA content and apoptosis, in several types of myocardial hypertrophy in order to define the biological characteristics of this process. Methods: The biological parameters were investigated in normal hearts (n=4) and in 21 cases of left ventricular myocardial hypertrophy related to pressure overload (n=7), post-infarction remodeling (n=8) and hypertrophic cardiomyopathy (HCM) (n=8). Results: The analyzed biomarkers were similar in hypertension and in remodeling, with a very high apoptotic index (mean values: 8.1 and 8.5%, respectively), a low PCNA positivity (mean values: 1.8 and 1.6%) and a prevalent diploid DNA content (DNA index: 1.2). Conversely, HCM showed a high mean PCNA index (21.2%) associated with a prevalence of hyperdiploid myocytes (DNA index: 1.8) and a low number of apoptotic cells (mean value: 1.7%). Conclusions: There are significant biological differences between hypertrophy in HCM and that related to arterial hypertension and post-infarction remodeling. Therefore, the combined evaluation of DNA content, PCNA and apoptotic indices could provide a powerful diagnostic tool in doubtful cases of myocardial primary or secondary hypertrophy and open new avenues in the clinical treatment of these entities.Fil: Matturri, Luigi. Università degli Studi di Milano; ItaliaFil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador; ArgentinaFil: Lavezzi, Anna Maria. Università degli Studi di Milano; ItaliaElsevier Ireland2002-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/181923Matturri, Luigi; Milei, Jose; Grana, Daniel Rodolfo; Lavezzi, Anna Maria; Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index; Elsevier Ireland; International Journal of Cardiology; 82; 1; 1-2002; 33-390167-5273CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167527301005782info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-5273(01)00578-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:31Zoai:ri.conicet.gov.ar:11336/181923instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:31.532CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
title Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
spellingShingle Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
Matturri, Luigi
APOPTOSIS
DNA
MYOCARDIAL HYPERTROPHY
PROLIFERATING CELL NUCLEAR ANTIGEN
title_short Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
title_full Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
title_fullStr Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
title_full_unstemmed Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
title_sort Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index
dc.creator.none.fl_str_mv Matturri, Luigi
Milei, Jose
Grana, Daniel Rodolfo
Lavezzi, Anna Maria
author Matturri, Luigi
author_facet Matturri, Luigi
Milei, Jose
Grana, Daniel Rodolfo
Lavezzi, Anna Maria
author_role author
author2 Milei, Jose
Grana, Daniel Rodolfo
Lavezzi, Anna Maria
author2_role author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
DNA
MYOCARDIAL HYPERTROPHY
PROLIFERATING CELL NUCLEAR ANTIGEN
topic APOPTOSIS
DNA
MYOCARDIAL HYPERTROPHY
PROLIFERATING CELL NUCLEAR ANTIGEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: At present little is known about the biological basis of cellular alterations in myocardial hypertrophy. The present study aims to analyze proliferating cell nuclear antigen (PCNA) expression, DNA content and apoptosis, in several types of myocardial hypertrophy in order to define the biological characteristics of this process. Methods: The biological parameters were investigated in normal hearts (n=4) and in 21 cases of left ventricular myocardial hypertrophy related to pressure overload (n=7), post-infarction remodeling (n=8) and hypertrophic cardiomyopathy (HCM) (n=8). Results: The analyzed biomarkers were similar in hypertension and in remodeling, with a very high apoptotic index (mean values: 8.1 and 8.5%, respectively), a low PCNA positivity (mean values: 1.8 and 1.6%) and a prevalent diploid DNA content (DNA index: 1.2). Conversely, HCM showed a high mean PCNA index (21.2%) associated with a prevalence of hyperdiploid myocytes (DNA index: 1.8) and a low number of apoptotic cells (mean value: 1.7%). Conclusions: There are significant biological differences between hypertrophy in HCM and that related to arterial hypertension and post-infarction remodeling. Therefore, the combined evaluation of DNA content, PCNA and apoptotic indices could provide a powerful diagnostic tool in doubtful cases of myocardial primary or secondary hypertrophy and open new avenues in the clinical treatment of these entities.
Fil: Matturri, Luigi. Università degli Studi di Milano; Italia
Fil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Grana, Daniel Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador; Argentina
Fil: Lavezzi, Anna Maria. Università degli Studi di Milano; Italia
description Background: At present little is known about the biological basis of cellular alterations in myocardial hypertrophy. The present study aims to analyze proliferating cell nuclear antigen (PCNA) expression, DNA content and apoptosis, in several types of myocardial hypertrophy in order to define the biological characteristics of this process. Methods: The biological parameters were investigated in normal hearts (n=4) and in 21 cases of left ventricular myocardial hypertrophy related to pressure overload (n=7), post-infarction remodeling (n=8) and hypertrophic cardiomyopathy (HCM) (n=8). Results: The analyzed biomarkers were similar in hypertension and in remodeling, with a very high apoptotic index (mean values: 8.1 and 8.5%, respectively), a low PCNA positivity (mean values: 1.8 and 1.6%) and a prevalent diploid DNA content (DNA index: 1.2). Conversely, HCM showed a high mean PCNA index (21.2%) associated with a prevalence of hyperdiploid myocytes (DNA index: 1.8) and a low number of apoptotic cells (mean value: 1.7%). Conclusions: There are significant biological differences between hypertrophy in HCM and that related to arterial hypertension and post-infarction remodeling. Therefore, the combined evaluation of DNA content, PCNA and apoptotic indices could provide a powerful diagnostic tool in doubtful cases of myocardial primary or secondary hypertrophy and open new avenues in the clinical treatment of these entities.
publishDate 2002
dc.date.none.fl_str_mv 2002-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181923
Matturri, Luigi; Milei, Jose; Grana, Daniel Rodolfo; Lavezzi, Anna Maria; Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index; Elsevier Ireland; International Journal of Cardiology; 82; 1; 1-2002; 33-39
0167-5273
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181923
identifier_str_mv Matturri, Luigi; Milei, Jose; Grana, Daniel Rodolfo; Lavezzi, Anna Maria; Characterization of myocardial hypertrophy by DNA content, PCNA expression and apoptotic index; Elsevier Ireland; International Journal of Cardiology; 82; 1; 1-2002; 33-39
0167-5273
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0167527301005782
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-5273(01)00578-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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