Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water

Autores
Ossana, Natalia Alejandra; Eissa, Bettina Lorena; Baudou, Federico Gastón; Castañé, Patricia Mónica; Soloneski, Sonia Maria Elsa; Ferrari, Lucrecia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this paper is to assess the water quality to chemical pollution at Roggero Dam, the headwater of the Reconquista river, and to perform a Cadmium (Cd) contamination pulse simulation through a wide battery of biomarkers which included: genotoxicity and enzymatic biomarker parameters on a neotropical teleost fish namely Cnesterodon decemmaculatus. Water samples were taken in order to determine the river's physicochemical profile. An integrative approach was applied using a biomarker index. The bioassay involved the use of laboratory culture adult animals, acclimatized in moderately hard water (MHW) and fed ad libitum. A semi-static 96 h bioassay was conducted and the experimental groups were as follows: [1] river water (Rg); [2] river water + 2 mg/L Cd (RgCd); [3] MHW + 2 mg/L Cadmium (Cd), positive metal control; [4] MHW + 5 mg/L Cyclophosphamide (positive genotoxicity control -CP); [5] MWH, negative control (NC). At the end of the exposure time fishes were sectioned and the following biomarkers were determined: 1) condition factor rate (CF); 2) for the anterior section (A) (head): glutathione (GSH) and protein (Pr) content; 3) for the body midsection (M) (viscera): Pr, GSH, Glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD). Blood samples were also taken from the fish specimens to estimate the frequency of micronuclei (MN) as well as other nuclear abnormalities (NA). The physicochemical profile of the river water sample indicated high Copper concentrations. CAT and SOD activity and total Pr content did not show any significant changes. GST activity decreased in fish exposed to Rg, while GSH content decreased significantly for all treatments compared to controls in MHW. These results would seem to point to a reduction in cell defense capability as a result of the depletion antioxidants such as GSH. The NA frequency increased significantly in all treated groups while MN frequency was increased only in Cd and CP groups. Using some the biomarkers measured, a biomarker index was estimated which revealed that fish exposed to Rg were 90% affected or highly affected, while those exposed to RgCd were 80% and Cd 68% affected or highly affected. The obtained results indicate the usefulness of the use of a battery of variables by means of the biomarker index to analyze water quality.
Fil: Ossana, Natalia Alejandra. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
Fil: Eissa, Bettina Lorena. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
Fil: Baudou, Federico Gastón. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina
Fil: Castañé, Patricia Mónica. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ferrari, Lucrecia. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
BIOMARKER INDEX
CADMIUM
CNESTERODON DECEMMACULATUS
GENOTOXICITY
OXIDATIVE STRESS
RECONQUISTA RIVER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114765

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network_name_str CONICET Digital (CONICET)
spelling Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river waterOssana, Natalia AlejandraEissa, Bettina LorenaBaudou, Federico GastónCastañé, Patricia MónicaSoloneski, Sonia Maria ElsaFerrari, LucreciaBIOMARKER INDEXCADMIUMCNESTERODON DECEMMACULATUSGENOTOXICITYOXIDATIVE STRESSRECONQUISTA RIVERhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The aim of this paper is to assess the water quality to chemical pollution at Roggero Dam, the headwater of the Reconquista river, and to perform a Cadmium (Cd) contamination pulse simulation through a wide battery of biomarkers which included: genotoxicity and enzymatic biomarker parameters on a neotropical teleost fish namely Cnesterodon decemmaculatus. Water samples were taken in order to determine the river's physicochemical profile. An integrative approach was applied using a biomarker index. The bioassay involved the use of laboratory culture adult animals, acclimatized in moderately hard water (MHW) and fed ad libitum. A semi-static 96 h bioassay was conducted and the experimental groups were as follows: [1] river water (Rg); [2] river water + 2 mg/L Cd (RgCd); [3] MHW + 2 mg/L Cadmium (Cd), positive metal control; [4] MHW + 5 mg/L Cyclophosphamide (positive genotoxicity control -CP); [5] MWH, negative control (NC). At the end of the exposure time fishes were sectioned and the following biomarkers were determined: 1) condition factor rate (CF); 2) for the anterior section (A) (head): glutathione (GSH) and protein (Pr) content; 3) for the body midsection (M) (viscera): Pr, GSH, Glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD). Blood samples were also taken from the fish specimens to estimate the frequency of micronuclei (MN) as well as other nuclear abnormalities (NA). The physicochemical profile of the river water sample indicated high Copper concentrations. CAT and SOD activity and total Pr content did not show any significant changes. GST activity decreased in fish exposed to Rg, while GSH content decreased significantly for all treatments compared to controls in MHW. These results would seem to point to a reduction in cell defense capability as a result of the depletion antioxidants such as GSH. The NA frequency increased significantly in all treated groups while MN frequency was increased only in Cd and CP groups. Using some the biomarkers measured, a biomarker index was estimated which revealed that fish exposed to Rg were 90% affected or highly affected, while those exposed to RgCd were 80% and Cd 68% affected or highly affected. The obtained results indicate the usefulness of the use of a battery of variables by means of the biomarker index to analyze water quality.Fil: Ossana, Natalia Alejandra. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; ArgentinaFil: Eissa, Bettina Lorena. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; ArgentinaFil: Baudou, Federico Gastón. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; ArgentinaFil: Castañé, Patricia Mónica. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; ArgentinaFil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Ferrari, Lucrecia. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaAcademic Press Inc Elsevier Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114765Ossana, Natalia Alejandra; Eissa, Bettina Lorena; Baudou, Federico Gastón; Castañé, Patricia Mónica; Soloneski, Sonia Maria Elsa; et al.; Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 133; 11-2016; 73-810147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0147651316302524info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2016.06.046info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:36Zoai:ri.conicet.gov.ar:11336/114765instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:36.31CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
title Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
spellingShingle Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
Ossana, Natalia Alejandra
BIOMARKER INDEX
CADMIUM
CNESTERODON DECEMMACULATUS
GENOTOXICITY
OXIDATIVE STRESS
RECONQUISTA RIVER
title_short Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
title_full Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
title_fullStr Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
title_full_unstemmed Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
title_sort Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water
dc.creator.none.fl_str_mv Ossana, Natalia Alejandra
Eissa, Bettina Lorena
Baudou, Federico Gastón
Castañé, Patricia Mónica
Soloneski, Sonia Maria Elsa
Ferrari, Lucrecia
author Ossana, Natalia Alejandra
author_facet Ossana, Natalia Alejandra
Eissa, Bettina Lorena
Baudou, Federico Gastón
Castañé, Patricia Mónica
Soloneski, Sonia Maria Elsa
Ferrari, Lucrecia
author_role author
author2 Eissa, Bettina Lorena
Baudou, Federico Gastón
Castañé, Patricia Mónica
Soloneski, Sonia Maria Elsa
Ferrari, Lucrecia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOMARKER INDEX
CADMIUM
CNESTERODON DECEMMACULATUS
GENOTOXICITY
OXIDATIVE STRESS
RECONQUISTA RIVER
topic BIOMARKER INDEX
CADMIUM
CNESTERODON DECEMMACULATUS
GENOTOXICITY
OXIDATIVE STRESS
RECONQUISTA RIVER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this paper is to assess the water quality to chemical pollution at Roggero Dam, the headwater of the Reconquista river, and to perform a Cadmium (Cd) contamination pulse simulation through a wide battery of biomarkers which included: genotoxicity and enzymatic biomarker parameters on a neotropical teleost fish namely Cnesterodon decemmaculatus. Water samples were taken in order to determine the river's physicochemical profile. An integrative approach was applied using a biomarker index. The bioassay involved the use of laboratory culture adult animals, acclimatized in moderately hard water (MHW) and fed ad libitum. A semi-static 96 h bioassay was conducted and the experimental groups were as follows: [1] river water (Rg); [2] river water + 2 mg/L Cd (RgCd); [3] MHW + 2 mg/L Cadmium (Cd), positive metal control; [4] MHW + 5 mg/L Cyclophosphamide (positive genotoxicity control -CP); [5] MWH, negative control (NC). At the end of the exposure time fishes were sectioned and the following biomarkers were determined: 1) condition factor rate (CF); 2) for the anterior section (A) (head): glutathione (GSH) and protein (Pr) content; 3) for the body midsection (M) (viscera): Pr, GSH, Glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD). Blood samples were also taken from the fish specimens to estimate the frequency of micronuclei (MN) as well as other nuclear abnormalities (NA). The physicochemical profile of the river water sample indicated high Copper concentrations. CAT and SOD activity and total Pr content did not show any significant changes. GST activity decreased in fish exposed to Rg, while GSH content decreased significantly for all treatments compared to controls in MHW. These results would seem to point to a reduction in cell defense capability as a result of the depletion antioxidants such as GSH. The NA frequency increased significantly in all treated groups while MN frequency was increased only in Cd and CP groups. Using some the biomarkers measured, a biomarker index was estimated which revealed that fish exposed to Rg were 90% affected or highly affected, while those exposed to RgCd were 80% and Cd 68% affected or highly affected. The obtained results indicate the usefulness of the use of a battery of variables by means of the biomarker index to analyze water quality.
Fil: Ossana, Natalia Alejandra. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
Fil: Eissa, Bettina Lorena. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
Fil: Baudou, Federico Gastón. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina
Fil: Castañé, Patricia Mónica. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ferrari, Lucrecia. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description The aim of this paper is to assess the water quality to chemical pollution at Roggero Dam, the headwater of the Reconquista river, and to perform a Cadmium (Cd) contamination pulse simulation through a wide battery of biomarkers which included: genotoxicity and enzymatic biomarker parameters on a neotropical teleost fish namely Cnesterodon decemmaculatus. Water samples were taken in order to determine the river's physicochemical profile. An integrative approach was applied using a biomarker index. The bioassay involved the use of laboratory culture adult animals, acclimatized in moderately hard water (MHW) and fed ad libitum. A semi-static 96 h bioassay was conducted and the experimental groups were as follows: [1] river water (Rg); [2] river water + 2 mg/L Cd (RgCd); [3] MHW + 2 mg/L Cadmium (Cd), positive metal control; [4] MHW + 5 mg/L Cyclophosphamide (positive genotoxicity control -CP); [5] MWH, negative control (NC). At the end of the exposure time fishes were sectioned and the following biomarkers were determined: 1) condition factor rate (CF); 2) for the anterior section (A) (head): glutathione (GSH) and protein (Pr) content; 3) for the body midsection (M) (viscera): Pr, GSH, Glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD). Blood samples were also taken from the fish specimens to estimate the frequency of micronuclei (MN) as well as other nuclear abnormalities (NA). The physicochemical profile of the river water sample indicated high Copper concentrations. CAT and SOD activity and total Pr content did not show any significant changes. GST activity decreased in fish exposed to Rg, while GSH content decreased significantly for all treatments compared to controls in MHW. These results would seem to point to a reduction in cell defense capability as a result of the depletion antioxidants such as GSH. The NA frequency increased significantly in all treated groups while MN frequency was increased only in Cd and CP groups. Using some the biomarkers measured, a biomarker index was estimated which revealed that fish exposed to Rg were 90% affected or highly affected, while those exposed to RgCd were 80% and Cd 68% affected or highly affected. The obtained results indicate the usefulness of the use of a battery of variables by means of the biomarker index to analyze water quality.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114765
Ossana, Natalia Alejandra; Eissa, Bettina Lorena; Baudou, Federico Gastón; Castañé, Patricia Mónica; Soloneski, Sonia Maria Elsa; et al.; Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 133; 11-2016; 73-81
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114765
identifier_str_mv Ossana, Natalia Alejandra; Eissa, Bettina Lorena; Baudou, Federico Gastón; Castañé, Patricia Mónica; Soloneski, Sonia Maria Elsa; et al.; Multibiomarker response in ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842) exposed to Reconquista river water; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 133; 11-2016; 73-81
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2016.06.046
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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publisher.none.fl_str_mv Academic Press Inc Elsevier Science
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