First description of seronegative HTLV-1 carriers in Argentina

Autores
Gallego, Sandra Veronica; Frutos, Maria Celia; Blanco, Sebastian; Castro, Gonzalo; Balangero, Marcos Cesar; Panigo, David Elías; Mangeaud, Arnaldo; Remondegui, Carlos; Rocha, Anderson Santos; Franco, Gabriela Melo; Martins, Marina Lobato; Barbosa Stancioli, Edel Figueiredo; Nates, Silvia Viviana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In some areas of Argentina endemic for human T-lymphotropic virus type 1 (HTLV-1), tropical spastic paraparesis is frequent in subjects who lack antibodies against the virus; however, the relevance of this seronegative status in the country has not been investigated. In neighboring countries, HTLV-1 seronegative status has been described in patients with different diseases; however, data regarding features of seronegative HTLV-1 carriers are scarce. We investigated the seronegative status in 124 relatives of 28 HTLV-1 infected subjects from an endemic area in Northwest Argentina. Blood samples and clinical/epidemiological data were collected. Human T-lymphotropic virus type 1 infection was diagnosed by serology and long terminal repeat (LTR) sequence, env and tax gene detection. IgG anti-Tax HTLV-1 antibody, tax gene sequence, and DNA proviral load were also evaluated. Seventy-five percent of the 124 relatives were negative for HTLV-1/2 antibodies; 35.5% were also negative by molecular assays and 64.5% were negative for HTLV-1 LTR and env sequences, but positive for two sequences of HTLV-1 tax gene. Also, 35.7% of these subjects had IgG anti-Tax antibodies. The seronegative HTLV-1 status was significantly associated with male gender, youth, and sensory symptoms/autonomic nervous system dysfunction. High rates of seronegative symptomatic and asymptomatic HTLV-1 carriers in Argentina are described. The evidence highlights that HTLV-1 prevalence may be underestimated worldwide. Larger cohort studies are required to assess disease outcome in these seronegative subjects. Also, the findings emphasize the limitations of ongoing screening assays for diagnosis and blood safety. Therefore, algorithms for HTLV-1 diagnosis should include not only serological but also molecular assays.
Fil: Gallego, Sandra Veronica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Frutos, Maria Celia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Blanco, Sebastian. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Castro, Gonzalo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Balangero, Marcos Cesar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Panigo, David Elías. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina
Fil: Mangeaud, Arnaldo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina
Fil: Remondegui, Carlos. Hospital San Roque. Servicio de Infectología y Enfermedades Tropicales; Argentina
Fil: Rocha, Anderson Santos. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; Brasil
Fil: Franco, Gabriela Melo. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; Brasil
Fil: Martins, Marina Lobato. Interdisciplinary HTLV Research Group; Brasil
Fil: Barbosa Stancioli, Edel Figueiredo. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; Brasil
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Materia
Seronegative HTLV-1 carriers
Tax gene
HTLV-1 endemic zone
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132024
Acceder
id CONICETDig_d124e924d93c6c646f97a6565be61bfe
oai_identifier_str oai:ri.conicet.gov.ar:11336/132024
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First description of seronegative HTLV-1 carriers in ArgentinaGallego, Sandra VeronicaFrutos, Maria CeliaBlanco, SebastianCastro, GonzaloBalangero, Marcos CesarPanigo, David ElíasMangeaud, ArnaldoRemondegui, CarlosRocha, Anderson SantosFranco, Gabriela MeloMartins, Marina LobatoBarbosa Stancioli, Edel FigueiredoNates, Silvia VivianaSeronegative HTLV-1 carriersTax geneHTLV-1 endemic zoneArgentinahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In some areas of Argentina endemic for human T-lymphotropic virus type 1 (HTLV-1), tropical spastic paraparesis is frequent in subjects who lack antibodies against the virus; however, the relevance of this seronegative status in the country has not been investigated. In neighboring countries, HTLV-1 seronegative status has been described in patients with different diseases; however, data regarding features of seronegative HTLV-1 carriers are scarce. We investigated the seronegative status in 124 relatives of 28 HTLV-1 infected subjects from an endemic area in Northwest Argentina. Blood samples and clinical/epidemiological data were collected. Human T-lymphotropic virus type 1 infection was diagnosed by serology and long terminal repeat (LTR) sequence, env and tax gene detection. IgG anti-Tax HTLV-1 antibody, tax gene sequence, and DNA proviral load were also evaluated. Seventy-five percent of the 124 relatives were negative for HTLV-1/2 antibodies; 35.5% were also negative by molecular assays and 64.5% were negative for HTLV-1 LTR and env sequences, but positive for two sequences of HTLV-1 tax gene. Also, 35.7% of these subjects had IgG anti-Tax antibodies. The seronegative HTLV-1 status was significantly associated with male gender, youth, and sensory symptoms/autonomic nervous system dysfunction. High rates of seronegative symptomatic and asymptomatic HTLV-1 carriers in Argentina are described. The evidence highlights that HTLV-1 prevalence may be underestimated worldwide. Larger cohort studies are required to assess disease outcome in these seronegative subjects. Also, the findings emphasize the limitations of ongoing screening assays for diagnosis and blood safety. Therefore, algorithms for HTLV-1 diagnosis should include not only serological but also molecular assays.Fil: Gallego, Sandra Veronica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Frutos, Maria Celia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Blanco, Sebastian. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Castro, Gonzalo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Balangero, Marcos Cesar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Panigo, David Elías. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; ArgentinaFil: Mangeaud, Arnaldo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; ArgentinaFil: Remondegui, Carlos. Hospital San Roque. Servicio de Infectología y Enfermedades Tropicales; ArgentinaFil: Rocha, Anderson Santos. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; BrasilFil: Franco, Gabriela Melo. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; BrasilFil: Martins, Marina Lobato. Interdisciplinary HTLV Research Group; BrasilFil: Barbosa Stancioli, Edel Figueiredo. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; BrasilFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaAmerican Society of Tropical Medicine and Hygiene2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132024Gallego, Sandra Veronica; Frutos, Maria Celia; Blanco, Sebastian; Castro, Gonzalo; Balangero, Marcos Cesar; et al.; First description of seronegative HTLV-1 carriers in Argentina; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 102; 4; 2-2020; 889-8950002-96371476-1645CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.19-0647info:eu-repo/semantics/altIdentifier/url/https://www.ajtmh.org/view/journals/tpmd/102/4/article-p889.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:37Zoai:ri.conicet.gov.ar:11336/132024instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:37.379CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First description of seronegative HTLV-1 carriers in Argentina
title First description of seronegative HTLV-1 carriers in Argentina
spellingShingle First description of seronegative HTLV-1 carriers in Argentina
Gallego, Sandra Veronica
Seronegative HTLV-1 carriers
Tax gene
HTLV-1 endemic zone
Argentina
title_short First description of seronegative HTLV-1 carriers in Argentina
title_full First description of seronegative HTLV-1 carriers in Argentina
title_fullStr First description of seronegative HTLV-1 carriers in Argentina
title_full_unstemmed First description of seronegative HTLV-1 carriers in Argentina
title_sort First description of seronegative HTLV-1 carriers in Argentina
dc.creator.none.fl_str_mv Gallego, Sandra Veronica
Frutos, Maria Celia
Blanco, Sebastian
Castro, Gonzalo
Balangero, Marcos Cesar
Panigo, David Elías
Mangeaud, Arnaldo
Remondegui, Carlos
Rocha, Anderson Santos
Franco, Gabriela Melo
Martins, Marina Lobato
Barbosa Stancioli, Edel Figueiredo
Nates, Silvia Viviana
author Gallego, Sandra Veronica
author_facet Gallego, Sandra Veronica
Frutos, Maria Celia
Blanco, Sebastian
Castro, Gonzalo
Balangero, Marcos Cesar
Panigo, David Elías
Mangeaud, Arnaldo
Remondegui, Carlos
Rocha, Anderson Santos
Franco, Gabriela Melo
Martins, Marina Lobato
Barbosa Stancioli, Edel Figueiredo
Nates, Silvia Viviana
author_role author
author2 Frutos, Maria Celia
Blanco, Sebastian
Castro, Gonzalo
Balangero, Marcos Cesar
Panigo, David Elías
Mangeaud, Arnaldo
Remondegui, Carlos
Rocha, Anderson Santos
Franco, Gabriela Melo
Martins, Marina Lobato
Barbosa Stancioli, Edel Figueiredo
Nates, Silvia Viviana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Seronegative HTLV-1 carriers
Tax gene
HTLV-1 endemic zone
Argentina
topic Seronegative HTLV-1 carriers
Tax gene
HTLV-1 endemic zone
Argentina
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In some areas of Argentina endemic for human T-lymphotropic virus type 1 (HTLV-1), tropical spastic paraparesis is frequent in subjects who lack antibodies against the virus; however, the relevance of this seronegative status in the country has not been investigated. In neighboring countries, HTLV-1 seronegative status has been described in patients with different diseases; however, data regarding features of seronegative HTLV-1 carriers are scarce. We investigated the seronegative status in 124 relatives of 28 HTLV-1 infected subjects from an endemic area in Northwest Argentina. Blood samples and clinical/epidemiological data were collected. Human T-lymphotropic virus type 1 infection was diagnosed by serology and long terminal repeat (LTR) sequence, env and tax gene detection. IgG anti-Tax HTLV-1 antibody, tax gene sequence, and DNA proviral load were also evaluated. Seventy-five percent of the 124 relatives were negative for HTLV-1/2 antibodies; 35.5% were also negative by molecular assays and 64.5% were negative for HTLV-1 LTR and env sequences, but positive for two sequences of HTLV-1 tax gene. Also, 35.7% of these subjects had IgG anti-Tax antibodies. The seronegative HTLV-1 status was significantly associated with male gender, youth, and sensory symptoms/autonomic nervous system dysfunction. High rates of seronegative symptomatic and asymptomatic HTLV-1 carriers in Argentina are described. The evidence highlights that HTLV-1 prevalence may be underestimated worldwide. Larger cohort studies are required to assess disease outcome in these seronegative subjects. Also, the findings emphasize the limitations of ongoing screening assays for diagnosis and blood safety. Therefore, algorithms for HTLV-1 diagnosis should include not only serological but also molecular assays.
Fil: Gallego, Sandra Veronica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Frutos, Maria Celia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Blanco, Sebastian. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Castro, Gonzalo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Balangero, Marcos Cesar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Panigo, David Elías. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina
Fil: Mangeaud, Arnaldo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina
Fil: Remondegui, Carlos. Hospital San Roque. Servicio de Infectología y Enfermedades Tropicales; Argentina
Fil: Rocha, Anderson Santos. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; Brasil
Fil: Franco, Gabriela Melo. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; Brasil
Fil: Martins, Marina Lobato. Interdisciplinary HTLV Research Group; Brasil
Fil: Barbosa Stancioli, Edel Figueiredo. Universidad Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Microbiología. Laboratorio de Virología Básica y Aplicada; Brasil. Interdisciplinary HTLV Research Group; Brasil
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
description In some areas of Argentina endemic for human T-lymphotropic virus type 1 (HTLV-1), tropical spastic paraparesis is frequent in subjects who lack antibodies against the virus; however, the relevance of this seronegative status in the country has not been investigated. In neighboring countries, HTLV-1 seronegative status has been described in patients with different diseases; however, data regarding features of seronegative HTLV-1 carriers are scarce. We investigated the seronegative status in 124 relatives of 28 HTLV-1 infected subjects from an endemic area in Northwest Argentina. Blood samples and clinical/epidemiological data were collected. Human T-lymphotropic virus type 1 infection was diagnosed by serology and long terminal repeat (LTR) sequence, env and tax gene detection. IgG anti-Tax HTLV-1 antibody, tax gene sequence, and DNA proviral load were also evaluated. Seventy-five percent of the 124 relatives were negative for HTLV-1/2 antibodies; 35.5% were also negative by molecular assays and 64.5% were negative for HTLV-1 LTR and env sequences, but positive for two sequences of HTLV-1 tax gene. Also, 35.7% of these subjects had IgG anti-Tax antibodies. The seronegative HTLV-1 status was significantly associated with male gender, youth, and sensory symptoms/autonomic nervous system dysfunction. High rates of seronegative symptomatic and asymptomatic HTLV-1 carriers in Argentina are described. The evidence highlights that HTLV-1 prevalence may be underestimated worldwide. Larger cohort studies are required to assess disease outcome in these seronegative subjects. Also, the findings emphasize the limitations of ongoing screening assays for diagnosis and blood safety. Therefore, algorithms for HTLV-1 diagnosis should include not only serological but also molecular assays.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132024
Gallego, Sandra Veronica; Frutos, Maria Celia; Blanco, Sebastian; Castro, Gonzalo; Balangero, Marcos Cesar; et al.; First description of seronegative HTLV-1 carriers in Argentina; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 102; 4; 2-2020; 889-895
0002-9637
1476-1645
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132024
identifier_str_mv Gallego, Sandra Veronica; Frutos, Maria Celia; Blanco, Sebastian; Castro, Gonzalo; Balangero, Marcos Cesar; et al.; First description of seronegative HTLV-1 carriers in Argentina; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 102; 4; 2-2020; 889-895
0002-9637
1476-1645
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.19-0647
info:eu-repo/semantics/altIdentifier/url/https://www.ajtmh.org/view/journals/tpmd/102/4/article-p889.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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