Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes

Autores
Cacace, Verónica Inés; Finkelsteyn, Andrés Gabriel; Tasso, Laura Mónica; Kusnier, Carlos Federico; Gomez, Karina Andrea; Fischbarg, Jorge
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 µM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 µM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na cotransporter and Cl bicarbonate exchanger in RVI, respectively.
Fil: Cacace, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Finkelsteyn, Andrés Gabriel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo; Argentina
Fil: Tasso, Laura Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Fischbarg, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Materia
CELLULAR VOLUMEN
HL-1 CARDIAC CELL
HYPOTONIC CONDITIONS
HYPERTONIC CONDITIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4008
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4008
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytesCacace, Verónica InésFinkelsteyn, Andrés GabrielTasso, Laura MónicaKusnier, Carlos FedericoGomez, Karina AndreaFischbarg, JorgeCELLULAR VOLUMENHL-1 CARDIAC CELLHYPOTONIC CONDITIONSHYPERTONIC CONDITIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 µM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 µM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na cotransporter and Cl bicarbonate exchanger in RVI, respectively.Fil: Cacace, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); ArgentinaFil: Finkelsteyn, Andrés Gabriel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo; ArgentinaFil: Tasso, Laura Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); ArgentinaFil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Fischbarg, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); ArgentinaKarger2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4008Cacace, Verónica Inés; Finkelsteyn, Andrés Gabriel; Tasso, Laura Mónica; Kusnier, Carlos Federico; Gomez, Karina Andrea; et al.; Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes; Karger; Cellular Physiology and Biochemistry; 33; 6; 7-2014; 1745-17571015-8987enginfo:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Article/FullText/362955info:eu-repo/semantics/altIdentifier/doi/DOI:10.1159/000362955info:eu-repo/semantics/altIdentifier/issn/1015-8987info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:56Zoai:ri.conicet.gov.ar:11336/4008instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:56.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
title Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
spellingShingle Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
Cacace, Verónica Inés
CELLULAR VOLUMEN
HL-1 CARDIAC CELL
HYPOTONIC CONDITIONS
HYPERTONIC CONDITIONS
title_short Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
title_full Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
title_fullStr Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
title_full_unstemmed Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
title_sort Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes
dc.creator.none.fl_str_mv Cacace, Verónica Inés
Finkelsteyn, Andrés Gabriel
Tasso, Laura Mónica
Kusnier, Carlos Federico
Gomez, Karina Andrea
Fischbarg, Jorge
author Cacace, Verónica Inés
author_facet Cacace, Verónica Inés
Finkelsteyn, Andrés Gabriel
Tasso, Laura Mónica
Kusnier, Carlos Federico
Gomez, Karina Andrea
Fischbarg, Jorge
author_role author
author2 Finkelsteyn, Andrés Gabriel
Tasso, Laura Mónica
Kusnier, Carlos Federico
Gomez, Karina Andrea
Fischbarg, Jorge
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CELLULAR VOLUMEN
HL-1 CARDIAC CELL
HYPOTONIC CONDITIONS
HYPERTONIC CONDITIONS
topic CELLULAR VOLUMEN
HL-1 CARDIAC CELL
HYPOTONIC CONDITIONS
HYPERTONIC CONDITIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 µM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 µM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na cotransporter and Cl bicarbonate exchanger in RVI, respectively.
Fil: Cacace, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Finkelsteyn, Andrés Gabriel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo; Argentina
Fil: Tasso, Laura Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Fischbarg, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
description Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 µM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 µM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na cotransporter and Cl bicarbonate exchanger in RVI, respectively.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4008
Cacace, Verónica Inés; Finkelsteyn, Andrés Gabriel; Tasso, Laura Mónica; Kusnier, Carlos Federico; Gomez, Karina Andrea; et al.; Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes; Karger; Cellular Physiology and Biochemistry; 33; 6; 7-2014; 1745-1757
1015-8987
url http://hdl.handle.net/11336/4008
identifier_str_mv Cacace, Verónica Inés; Finkelsteyn, Andrés Gabriel; Tasso, Laura Mónica; Kusnier, Carlos Federico; Gomez, Karina Andrea; et al.; Regulatory volume increase (RVI) and regulatory volume decrease (RVD) responses in HL-1 cardiac myocytes; Karger; Cellular Physiology and Biochemistry; 33; 6; 7-2014; 1745-1757
1015-8987
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.karger.com/Article/FullText/362955
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1159/000362955
info:eu-repo/semantics/altIdentifier/issn/1015-8987
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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