Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)

Autores
Moyetta, Natalia Rita; Ramos, Fabian; Leyria, Jimena; Canavoso, Lilian Etelvina; Fruttero, Leonardo Luis
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among special-ists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh prepara-tions, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.
Fil: Moyetta, Natalia Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Ramos, Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Canavoso, Lilian Etelvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
CLASSIFICATION
HEMOCYTE
MORPHOLOGY
TRIATOMINAE
ULTRASTRUCTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184606
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184606
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network_name_str CONICET Digital (CONICET)
spelling Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)Moyetta, Natalia RitaRamos, FabianLeyria, JimenaCanavoso, Lilian EtelvinaFruttero, Leonardo LuisCLASSIFICATIONHEMOCYTEMORPHOLOGYTRIATOMINAEULTRASTRUCTUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among special-ists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh prepara-tions, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.Fil: Moyetta, Natalia Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Ramos, Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Canavoso, Lilian Etelvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaMDPI AG2021-07-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184606Moyetta, Natalia Rita; Ramos, Fabian; Leyria, Jimena; Canavoso, Lilian Etelvina; Fruttero, Leonardo Luis; Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae); MDPI AG; Insects; 12; 7; 14-7-2021; 1-132075-4450CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4450/12/7/640info:eu-repo/semantics/altIdentifier/doi/10.3390/insects12070640info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:44:54Zoai:ri.conicet.gov.ar:11336/184606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:44:54.341CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
title Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
spellingShingle Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
Moyetta, Natalia Rita
CLASSIFICATION
HEMOCYTE
MORPHOLOGY
TRIATOMINAE
ULTRASTRUCTURE
title_short Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
title_full Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
title_fullStr Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
title_full_unstemmed Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
title_sort Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae)
dc.creator.none.fl_str_mv Moyetta, Natalia Rita
Ramos, Fabian
Leyria, Jimena
Canavoso, Lilian Etelvina
Fruttero, Leonardo Luis
author Moyetta, Natalia Rita
author_facet Moyetta, Natalia Rita
Ramos, Fabian
Leyria, Jimena
Canavoso, Lilian Etelvina
Fruttero, Leonardo Luis
author_role author
author2 Ramos, Fabian
Leyria, Jimena
Canavoso, Lilian Etelvina
Fruttero, Leonardo Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv CLASSIFICATION
HEMOCYTE
MORPHOLOGY
TRIATOMINAE
ULTRASTRUCTURE
topic CLASSIFICATION
HEMOCYTE
MORPHOLOGY
TRIATOMINAE
ULTRASTRUCTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among special-ists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh prepara-tions, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.
Fil: Moyetta, Natalia Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Ramos, Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Canavoso, Lilian Etelvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among special-ists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh prepara-tions, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184606
Moyetta, Natalia Rita; Ramos, Fabian; Leyria, Jimena; Canavoso, Lilian Etelvina; Fruttero, Leonardo Luis; Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae); MDPI AG; Insects; 12; 7; 14-7-2021; 1-13
2075-4450
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184606
identifier_str_mv Moyetta, Natalia Rita; Ramos, Fabian; Leyria, Jimena; Canavoso, Lilian Etelvina; Fruttero, Leonardo Luis; Morphological and ultrastructural characterization of hemocytes in an insect model, the hematophagous dipetalogaster maxima (Hemiptera: Reduviidae); MDPI AG; Insects; 12; 7; 14-7-2021; 1-13
2075-4450
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4450/12/7/640
info:eu-repo/semantics/altIdentifier/doi/10.3390/insects12070640
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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