Comparison between warm blood and crystalloid cardioplegia during open heart surgery

Autores
Ferreira, Ricardo; Fraga, César Guillermo; Carrasquedo, Fernando; Hourquebie, Hector; Grana, D.R.; Milei, Jose
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: This study was designed to compare the degree of myocardial protection afforded by warm blood and cold crystalloid cardioplegia in a group of patients undergoing elective coronary artery bypass surgery. Methods: Seventeen patients, were randomly assigned to Group A (n=9), who received crystalloid cardioplegic solution, and Group B who received warm blood cardioplegic solution (n=8). Before the aorta was clamped, and 10 min after reperfusion, blood samples from the coronary sinus were obtained to assay α-tocopherol, β-carotene, ubiquinol, and thiobarbituric acid reactive substances (TBARS). At the same intervals, biopsies from the left ventricle were obtained to determine ultrastructural alterations. Results: No significant changes were observed between preischemia and reperfusion values for both blood and crystalloid groups concerning α-tocopherol, β-carotene, and ubiquinol, and no differences between groups were detected. Values for TBARS in group A were 3.49±0.3 and 5.27±0.45 μM for presichemia and reperfusion samples, respectively (P<0.01). In group B values were 2.6±0.3 and 3.54±0.3 μM, respectively (P=NS). For electron microscopy studies, semiquantitative analysis showed a significant mitochondrial damage in reperfusion biopsies from group A (grades 0, 3 and 4). In group B, no significant changes were observed in mitochondrial damage between preischemia and repefusion biopsies (except for grade 0). Conclusion: These results indicate that blood cardioplegia affords better protection to the myocyte than crystalloid cardioplegia.
Fil: Ferreira, Ricardo. Ministerio de Defensa. Ejército Argentino. Hospital Militar Central Cirujano Mayor "Dr. Cosme Argerich"; Argentina
Fil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Carrasquedo, Fernando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Hourquebie, Hector. Ministerio de Defensa. Ejército Argentino. Hospital Militar Central Cirujano Mayor "Dr. Cosme Argerich"; Argentina
Fil: Grana, D.R.. Universidad del Salvador; Argentina
Fil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador; Argentina
Materia
CARDIOPLEGIA
CRYSTALLOID
HEART SURGERY
OXIDATIVE STRESS
ULTRASTRUCTURAL
WARM BLOOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125822
Acceder
id CONICETDig_d07a371cd38e61fab6a97737e9028e7c
oai_identifier_str oai:ri.conicet.gov.ar:11336/125822
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison between warm blood and crystalloid cardioplegia during open heart surgeryFerreira, RicardoFraga, César GuillermoCarrasquedo, FernandoHourquebie, HectorGrana, D.R.Milei, JoseCARDIOPLEGIACRYSTALLOIDHEART SURGERYOXIDATIVE STRESSULTRASTRUCTURALWARM BLOODhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Objective: This study was designed to compare the degree of myocardial protection afforded by warm blood and cold crystalloid cardioplegia in a group of patients undergoing elective coronary artery bypass surgery. Methods: Seventeen patients, were randomly assigned to Group A (n=9), who received crystalloid cardioplegic solution, and Group B who received warm blood cardioplegic solution (n=8). Before the aorta was clamped, and 10 min after reperfusion, blood samples from the coronary sinus were obtained to assay α-tocopherol, β-carotene, ubiquinol, and thiobarbituric acid reactive substances (TBARS). At the same intervals, biopsies from the left ventricle were obtained to determine ultrastructural alterations. Results: No significant changes were observed between preischemia and reperfusion values for both blood and crystalloid groups concerning α-tocopherol, β-carotene, and ubiquinol, and no differences between groups were detected. Values for TBARS in group A were 3.49±0.3 and 5.27±0.45 μM for presichemia and reperfusion samples, respectively (P<0.01). In group B values were 2.6±0.3 and 3.54±0.3 μM, respectively (P=NS). For electron microscopy studies, semiquantitative analysis showed a significant mitochondrial damage in reperfusion biopsies from group A (grades 0, 3 and 4). In group B, no significant changes were observed in mitochondrial damage between preischemia and repefusion biopsies (except for grade 0). Conclusion: These results indicate that blood cardioplegia affords better protection to the myocyte than crystalloid cardioplegia.Fil: Ferreira, Ricardo. Ministerio de Defensa. Ejército Argentino. Hospital Militar Central Cirujano Mayor "Dr. Cosme Argerich"; ArgentinaFil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Carrasquedo, Fernando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Hourquebie, Hector. Ministerio de Defensa. Ejército Argentino. Hospital Militar Central Cirujano Mayor "Dr. Cosme Argerich"; ArgentinaFil: Grana, D.R.. Universidad del Salvador; ArgentinaFil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador; ArgentinaElsevier Ireland2003-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125822Ferreira, Ricardo; Fraga, César Guillermo; Carrasquedo, Fernando; Hourquebie, Hector; Grana, D.R.; et al.; Comparison between warm blood and crystalloid cardioplegia during open heart surgery; Elsevier Ireland; International Journal of Cardiology; 90; 2-3; 9-2003; 253-2600167-5273CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/s0167-5273(02)00559-4info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167527302005594info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:13Zoai:ri.conicet.gov.ar:11336/125822instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:14.001CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison between warm blood and crystalloid cardioplegia during open heart surgery
title Comparison between warm blood and crystalloid cardioplegia during open heart surgery
spellingShingle Comparison between warm blood and crystalloid cardioplegia during open heart surgery
Ferreira, Ricardo
CARDIOPLEGIA
CRYSTALLOID
HEART SURGERY
OXIDATIVE STRESS
ULTRASTRUCTURAL
WARM BLOOD
title_short Comparison between warm blood and crystalloid cardioplegia during open heart surgery
title_full Comparison between warm blood and crystalloid cardioplegia during open heart surgery
title_fullStr Comparison between warm blood and crystalloid cardioplegia during open heart surgery
title_full_unstemmed Comparison between warm blood and crystalloid cardioplegia during open heart surgery
title_sort Comparison between warm blood and crystalloid cardioplegia during open heart surgery
dc.creator.none.fl_str_mv Ferreira, Ricardo
Fraga, César Guillermo
Carrasquedo, Fernando
Hourquebie, Hector
Grana, D.R.
Milei, Jose
author Ferreira, Ricardo
author_facet Ferreira, Ricardo
Fraga, César Guillermo
Carrasquedo, Fernando
Hourquebie, Hector
Grana, D.R.
Milei, Jose
author_role author
author2 Fraga, César Guillermo
Carrasquedo, Fernando
Hourquebie, Hector
Grana, D.R.
Milei, Jose
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CARDIOPLEGIA
CRYSTALLOID
HEART SURGERY
OXIDATIVE STRESS
ULTRASTRUCTURAL
WARM BLOOD
topic CARDIOPLEGIA
CRYSTALLOID
HEART SURGERY
OXIDATIVE STRESS
ULTRASTRUCTURAL
WARM BLOOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: This study was designed to compare the degree of myocardial protection afforded by warm blood and cold crystalloid cardioplegia in a group of patients undergoing elective coronary artery bypass surgery. Methods: Seventeen patients, were randomly assigned to Group A (n=9), who received crystalloid cardioplegic solution, and Group B who received warm blood cardioplegic solution (n=8). Before the aorta was clamped, and 10 min after reperfusion, blood samples from the coronary sinus were obtained to assay α-tocopherol, β-carotene, ubiquinol, and thiobarbituric acid reactive substances (TBARS). At the same intervals, biopsies from the left ventricle were obtained to determine ultrastructural alterations. Results: No significant changes were observed between preischemia and reperfusion values for both blood and crystalloid groups concerning α-tocopherol, β-carotene, and ubiquinol, and no differences between groups were detected. Values for TBARS in group A were 3.49±0.3 and 5.27±0.45 μM for presichemia and reperfusion samples, respectively (P<0.01). In group B values were 2.6±0.3 and 3.54±0.3 μM, respectively (P=NS). For electron microscopy studies, semiquantitative analysis showed a significant mitochondrial damage in reperfusion biopsies from group A (grades 0, 3 and 4). In group B, no significant changes were observed in mitochondrial damage between preischemia and repefusion biopsies (except for grade 0). Conclusion: These results indicate that blood cardioplegia affords better protection to the myocyte than crystalloid cardioplegia.
Fil: Ferreira, Ricardo. Ministerio de Defensa. Ejército Argentino. Hospital Militar Central Cirujano Mayor "Dr. Cosme Argerich"; Argentina
Fil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Carrasquedo, Fernando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Hourquebie, Hector. Ministerio de Defensa. Ejército Argentino. Hospital Militar Central Cirujano Mayor "Dr. Cosme Argerich"; Argentina
Fil: Grana, D.R.. Universidad del Salvador; Argentina
Fil: Milei, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad del Salvador; Argentina
description Objective: This study was designed to compare the degree of myocardial protection afforded by warm blood and cold crystalloid cardioplegia in a group of patients undergoing elective coronary artery bypass surgery. Methods: Seventeen patients, were randomly assigned to Group A (n=9), who received crystalloid cardioplegic solution, and Group B who received warm blood cardioplegic solution (n=8). Before the aorta was clamped, and 10 min after reperfusion, blood samples from the coronary sinus were obtained to assay α-tocopherol, β-carotene, ubiquinol, and thiobarbituric acid reactive substances (TBARS). At the same intervals, biopsies from the left ventricle were obtained to determine ultrastructural alterations. Results: No significant changes were observed between preischemia and reperfusion values for both blood and crystalloid groups concerning α-tocopherol, β-carotene, and ubiquinol, and no differences between groups were detected. Values for TBARS in group A were 3.49±0.3 and 5.27±0.45 μM for presichemia and reperfusion samples, respectively (P<0.01). In group B values were 2.6±0.3 and 3.54±0.3 μM, respectively (P=NS). For electron microscopy studies, semiquantitative analysis showed a significant mitochondrial damage in reperfusion biopsies from group A (grades 0, 3 and 4). In group B, no significant changes were observed in mitochondrial damage between preischemia and repefusion biopsies (except for grade 0). Conclusion: These results indicate that blood cardioplegia affords better protection to the myocyte than crystalloid cardioplegia.
publishDate 2003
dc.date.none.fl_str_mv 2003-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125822
Ferreira, Ricardo; Fraga, César Guillermo; Carrasquedo, Fernando; Hourquebie, Hector; Grana, D.R.; et al.; Comparison between warm blood and crystalloid cardioplegia during open heart surgery; Elsevier Ireland; International Journal of Cardiology; 90; 2-3; 9-2003; 253-260
0167-5273
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125822
identifier_str_mv Ferreira, Ricardo; Fraga, César Guillermo; Carrasquedo, Fernando; Hourquebie, Hector; Grana, D.R.; et al.; Comparison between warm blood and crystalloid cardioplegia during open heart surgery; Elsevier Ireland; International Journal of Cardiology; 90; 2-3; 9-2003; 253-260
0167-5273
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/s0167-5273(02)00559-4
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167527302005594
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269144659001344
score 13.13397

Publicaciones similares