Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other...

Autores
Chirino, Monica Gabriela; Fourastié, María Florencia; Centeno, Néstor Daniel; Bressa, Maria Jose
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heterochromatin may vary qualitatively, quantitatively, and in its location both in and between species. There were differences recorded in sex chromosomes, satellite sizes and location of C-bands in specimens of Lucilia sericata (Meigen, 1826) from three localities in the Buenos Aires region, Argentina (Bernal, Castelar, and Buenos Aires City). Mitotic analyses revealed the same diploid chromosome number of 2n = 10 + XY / XX (male / female) and the same size, morphology, and C-banding patterns on their autosomes. However, there are different morphotypes of X and Y chromosomes with a distinctive heterochromatin content in both arms. Four morphotypes of X (X1, X2, X3, and X4) and three of Y (Y1, Y2, and Y3) were recorded. The X metacentric and Y subtelocentric chromosomes were identified based on their very large and small sizes, respectively, and both had large heterochromatic blocks. These intraspecific differences are mainly due to quantitative variations in non-coding repetitive sequences located in the arms of both X and Y sex chromosomes. The specimens were very polymorphic both between and within the three localities sampled. Cytogenetic studies on specimens of L. sericata from the Nearctic, Palaearctic and Ethiopian regions also report differences in the morphology of the sex chromosomes and their C-banding patterns, some of which may have evolved independently. Our cytogenetic observations on L. sericata indicate that the accumulation of heterochromatin in the genome could be involved in chromosomal divergence and karyotype evolution of this species, as demonstrated in other Diptera. The polymorphic sex chromosomes are therefore important for understanding the evolution within species as well as speciation.
Fil: Chirino, Monica Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Entomología Aplicada y Forense; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fourastié, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Centeno, Néstor Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Entomología Aplicada y Forense; Argentina
Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
DIPTERA
CALLIPHORIDAE
LUCILIA SERICATA
INTRASPECIFIC VARIATION
CHROMOSOME POLYMORPHISM
CONSTITUTIVE HETEROCHROMATIN
ARGENTINA
CHROMOSOME EVOLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/177563
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspectsChirino, Monica GabrielaFourastié, María FlorenciaCenteno, Néstor DanielBressa, Maria JoseDIPTERACALLIPHORIDAELUCILIA SERICATAINTRASPECIFIC VARIATIONCHROMOSOME POLYMORPHISMCONSTITUTIVE HETEROCHROMATINARGENTINACHROMOSOME EVOLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heterochromatin may vary qualitatively, quantitatively, and in its location both in and between species. There were differences recorded in sex chromosomes, satellite sizes and location of C-bands in specimens of Lucilia sericata (Meigen, 1826) from three localities in the Buenos Aires region, Argentina (Bernal, Castelar, and Buenos Aires City). Mitotic analyses revealed the same diploid chromosome number of 2n = 10 + XY / XX (male / female) and the same size, morphology, and C-banding patterns on their autosomes. However, there are different morphotypes of X and Y chromosomes with a distinctive heterochromatin content in both arms. Four morphotypes of X (X1, X2, X3, and X4) and three of Y (Y1, Y2, and Y3) were recorded. The X metacentric and Y subtelocentric chromosomes were identified based on their very large and small sizes, respectively, and both had large heterochromatic blocks. These intraspecific differences are mainly due to quantitative variations in non-coding repetitive sequences located in the arms of both X and Y sex chromosomes. The specimens were very polymorphic both between and within the three localities sampled. Cytogenetic studies on specimens of L. sericata from the Nearctic, Palaearctic and Ethiopian regions also report differences in the morphology of the sex chromosomes and their C-banding patterns, some of which may have evolved independently. Our cytogenetic observations on L. sericata indicate that the accumulation of heterochromatin in the genome could be involved in chromosomal divergence and karyotype evolution of this species, as demonstrated in other Diptera. The polymorphic sex chromosomes are therefore important for understanding the evolution within species as well as speciation.Fil: Chirino, Monica Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Entomología Aplicada y Forense; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fourastié, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Centeno, Néstor Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Entomología Aplicada y Forense; ArgentinaFil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaCzech Academy of Sciences2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/177563Chirino, Monica Gabriela; Fourastié, María Florencia; Centeno, Néstor Daniel; Bressa, Maria Jose; Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects; Czech Academy of Sciences; European Journal of Entomology (Ceske Budejovice); 117; 7-2020; 295-3011210-5759CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.14411/eje.2020.034info:eu-repo/semantics/altIdentifier/url/https://www.eje.cz/artkey/eje-202001-0034_unusual_chromosome_polymorphism_and_heterochromatin_variation_in_the_argentinean_population_of_the_necrophagous.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:11Zoai:ri.conicet.gov.ar:11336/177563instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:12.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
title Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
spellingShingle Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
Chirino, Monica Gabriela
DIPTERA
CALLIPHORIDAE
LUCILIA SERICATA
INTRASPECIFIC VARIATION
CHROMOSOME POLYMORPHISM
CONSTITUTIVE HETEROCHROMATIN
ARGENTINA
CHROMOSOME EVOLUTION
title_short Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
title_full Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
title_fullStr Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
title_full_unstemmed Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
title_sort Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
dc.creator.none.fl_str_mv Chirino, Monica Gabriela
Fourastié, María Florencia
Centeno, Néstor Daniel
Bressa, Maria Jose
author Chirino, Monica Gabriela
author_facet Chirino, Monica Gabriela
Fourastié, María Florencia
Centeno, Néstor Daniel
Bressa, Maria Jose
author_role author
author2 Fourastié, María Florencia
Centeno, Néstor Daniel
Bressa, Maria Jose
author2_role author
author
author
dc.subject.none.fl_str_mv DIPTERA
CALLIPHORIDAE
LUCILIA SERICATA
INTRASPECIFIC VARIATION
CHROMOSOME POLYMORPHISM
CONSTITUTIVE HETEROCHROMATIN
ARGENTINA
CHROMOSOME EVOLUTION
topic DIPTERA
CALLIPHORIDAE
LUCILIA SERICATA
INTRASPECIFIC VARIATION
CHROMOSOME POLYMORPHISM
CONSTITUTIVE HETEROCHROMATIN
ARGENTINA
CHROMOSOME EVOLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heterochromatin may vary qualitatively, quantitatively, and in its location both in and between species. There were differences recorded in sex chromosomes, satellite sizes and location of C-bands in specimens of Lucilia sericata (Meigen, 1826) from three localities in the Buenos Aires region, Argentina (Bernal, Castelar, and Buenos Aires City). Mitotic analyses revealed the same diploid chromosome number of 2n = 10 + XY / XX (male / female) and the same size, morphology, and C-banding patterns on their autosomes. However, there are different morphotypes of X and Y chromosomes with a distinctive heterochromatin content in both arms. Four morphotypes of X (X1, X2, X3, and X4) and three of Y (Y1, Y2, and Y3) were recorded. The X metacentric and Y subtelocentric chromosomes were identified based on their very large and small sizes, respectively, and both had large heterochromatic blocks. These intraspecific differences are mainly due to quantitative variations in non-coding repetitive sequences located in the arms of both X and Y sex chromosomes. The specimens were very polymorphic both between and within the three localities sampled. Cytogenetic studies on specimens of L. sericata from the Nearctic, Palaearctic and Ethiopian regions also report differences in the morphology of the sex chromosomes and their C-banding patterns, some of which may have evolved independently. Our cytogenetic observations on L. sericata indicate that the accumulation of heterochromatin in the genome could be involved in chromosomal divergence and karyotype evolution of this species, as demonstrated in other Diptera. The polymorphic sex chromosomes are therefore important for understanding the evolution within species as well as speciation.
Fil: Chirino, Monica Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Entomología Aplicada y Forense; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fourastié, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Centeno, Néstor Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Entomología Aplicada y Forense; Argentina
Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description Heterochromatin may vary qualitatively, quantitatively, and in its location both in and between species. There were differences recorded in sex chromosomes, satellite sizes and location of C-bands in specimens of Lucilia sericata (Meigen, 1826) from three localities in the Buenos Aires region, Argentina (Bernal, Castelar, and Buenos Aires City). Mitotic analyses revealed the same diploid chromosome number of 2n = 10 + XY / XX (male / female) and the same size, morphology, and C-banding patterns on their autosomes. However, there are different morphotypes of X and Y chromosomes with a distinctive heterochromatin content in both arms. Four morphotypes of X (X1, X2, X3, and X4) and three of Y (Y1, Y2, and Y3) were recorded. The X metacentric and Y subtelocentric chromosomes were identified based on their very large and small sizes, respectively, and both had large heterochromatic blocks. These intraspecific differences are mainly due to quantitative variations in non-coding repetitive sequences located in the arms of both X and Y sex chromosomes. The specimens were very polymorphic both between and within the three localities sampled. Cytogenetic studies on specimens of L. sericata from the Nearctic, Palaearctic and Ethiopian regions also report differences in the morphology of the sex chromosomes and their C-banding patterns, some of which may have evolved independently. Our cytogenetic observations on L. sericata indicate that the accumulation of heterochromatin in the genome could be involved in chromosomal divergence and karyotype evolution of this species, as demonstrated in other Diptera. The polymorphic sex chromosomes are therefore important for understanding the evolution within species as well as speciation.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/177563
Chirino, Monica Gabriela; Fourastié, María Florencia; Centeno, Néstor Daniel; Bressa, Maria Jose; Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects; Czech Academy of Sciences; European Journal of Entomology (Ceske Budejovice); 117; 7-2020; 295-301
1210-5759
CONICET Digital
CONICET
url http://hdl.handle.net/11336/177563
identifier_str_mv Chirino, Monica Gabriela; Fourastié, María Florencia; Centeno, Néstor Daniel; Bressa, Maria Jose; Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects; Czech Academy of Sciences; European Journal of Entomology (Ceske Budejovice); 117; 7-2020; 295-301
1210-5759
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.14411/eje.2020.034
info:eu-repo/semantics/altIdentifier/url/https://www.eje.cz/artkey/eje-202001-0034_unusual_chromosome_polymorphism_and_heterochromatin_variation_in_the_argentinean_population_of_the_necrophagous.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Czech Academy of Sciences
publisher.none.fl_str_mv Czech Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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