Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation

Autores
Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; Buma, Anita G. J.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta.
Fil: Janknegt, Paul J.. University of Groningen; Países Bajos
Fil: De Graaff, C. Marco. Centre for Sustainable Water Technology; Países Bajos
Fil: Van De Poll, Willem H.. University of Groningen; Países Bajos
Fil: Visser, Ronald J. W.. University of Groningen; Países Bajos
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Buma, Anita G. J.. University of Groningen; Países Bajos
Materia
MICROALGAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94740

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spelling Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV RadiationJanknegt, Paul J.De Graaff, C. MarcoVan De Poll, Willem H.Visser, Ronald J. W.Helbling, Eduardo WalterBuma, Anita G. J.MICROALGAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta.Fil: Janknegt, Paul J.. University of Groningen; Países BajosFil: De Graaff, C. Marco. Centre for Sustainable Water Technology; Países BajosFil: Van De Poll, Willem H.. University of Groningen; Países BajosFil: Visser, Ronald J. W.. University of Groningen; Países BajosFil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; ArgentinaFil: Buma, Anita G. J.. University of Groningen; Países BajosWiley Blackwell Publishing, Inc2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94740Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; et al.; Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 85; 6; 11-2009; 1336-13450031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-1097.2009.00603.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2009.00603.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:53Zoai:ri.conicet.gov.ar:11336/94740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:54.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
title Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
spellingShingle Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
Janknegt, Paul J.
MICROALGAE
title_short Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
title_full Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
title_fullStr Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
title_full_unstemmed Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
title_sort Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
dc.creator.none.fl_str_mv Janknegt, Paul J.
De Graaff, C. Marco
Van De Poll, Willem H.
Visser, Ronald J. W.
Helbling, Eduardo Walter
Buma, Anita G. J.
author Janknegt, Paul J.
author_facet Janknegt, Paul J.
De Graaff, C. Marco
Van De Poll, Willem H.
Visser, Ronald J. W.
Helbling, Eduardo Walter
Buma, Anita G. J.
author_role author
author2 De Graaff, C. Marco
Van De Poll, Willem H.
Visser, Ronald J. W.
Helbling, Eduardo Walter
Buma, Anita G. J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MICROALGAE
topic MICROALGAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta.
Fil: Janknegt, Paul J.. University of Groningen; Países Bajos
Fil: De Graaff, C. Marco. Centre for Sustainable Water Technology; Países Bajos
Fil: Van De Poll, Willem H.. University of Groningen; Países Bajos
Fil: Visser, Ronald J. W.. University of Groningen; Países Bajos
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Buma, Anita G. J.. University of Groningen; Países Bajos
description Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94740
Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; et al.; Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 85; 6; 11-2009; 1336-1345
0031-8655
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94740
identifier_str_mv Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; et al.; Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 85; 6; 11-2009; 1336-1345
0031-8655
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-1097.2009.00603.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2009.00603.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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