Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation
- Autores
- Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; Buma, Anita G. J.
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta.
Fil: Janknegt, Paul J.. University of Groningen; Países Bajos
Fil: De Graaff, C. Marco. Centre for Sustainable Water Technology; Países Bajos
Fil: Van De Poll, Willem H.. University of Groningen; Países Bajos
Fil: Visser, Ronald J. W.. University of Groningen; Países Bajos
Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina
Fil: Buma, Anita G. J.. University of Groningen; Países Bajos - Materia
- MICROALGAE
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/94740
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Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV RadiationJanknegt, Paul J.De Graaff, C. MarcoVan De Poll, Willem H.Visser, Ronald J. W.Helbling, Eduardo WalterBuma, Anita G. J.MICROALGAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta.Fil: Janknegt, Paul J.. University of Groningen; Países BajosFil: De Graaff, C. Marco. Centre for Sustainable Water Technology; Países BajosFil: Van De Poll, Willem H.. University of Groningen; Países BajosFil: Visser, Ronald J. W.. University of Groningen; Países BajosFil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; ArgentinaFil: Buma, Anita G. J.. University of Groningen; Países BajosWiley Blackwell Publishing, Inc2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94740Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; et al.; Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 85; 6; 11-2009; 1336-13450031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-1097.2009.00603.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2009.00603.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:53Zoai:ri.conicet.gov.ar:11336/94740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:54.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
title |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
spellingShingle |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation Janknegt, Paul J. MICROALGAE |
title_short |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
title_full |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
title_fullStr |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
title_full_unstemmed |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
title_sort |
Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation |
dc.creator.none.fl_str_mv |
Janknegt, Paul J. De Graaff, C. Marco Van De Poll, Willem H. Visser, Ronald J. W. Helbling, Eduardo Walter Buma, Anita G. J. |
author |
Janknegt, Paul J. |
author_facet |
Janknegt, Paul J. De Graaff, C. Marco Van De Poll, Willem H. Visser, Ronald J. W. Helbling, Eduardo Walter Buma, Anita G. J. |
author_role |
author |
author2 |
De Graaff, C. Marco Van De Poll, Willem H. Visser, Ronald J. W. Helbling, Eduardo Walter Buma, Anita G. J. |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
MICROALGAE |
topic |
MICROALGAE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta. Fil: Janknegt, Paul J.. University of Groningen; Países Bajos Fil: De Graaff, C. Marco. Centre for Sustainable Water Technology; Países Bajos Fil: Van De Poll, Willem H.. University of Groningen; Países Bajos Fil: Visser, Ronald J. W.. University of Groningen; Países Bajos Fil: Helbling, Eduardo Walter. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Playa Unión. Estación de Fotobiología Playa Unión; Argentina Fil: Buma, Anita G. J.. University of Groningen; Países Bajos |
description |
Photoacclimation properties were investigated in two marine microalgae exposed to four ambient irradiance conditions: static photosynthetically active radiation (PAR: 400–700 nm), static PAR + UVR (280–700 nm), dynamic PAR and dynamic PAR + UVR. High light acclimated cultures of Thalassiosira weissflogii and Dunaliella tertiolecta were exposed outdoors for a maximum of 7 days. Dynamic irradiance was established by computer controlled vertical movement of 2 L bottles in a water filled basin. Immediate (<24 h), short‐term (1–3 days) and long‐term (4–7 days) photoacclimation was followed for antioxidants (superoxide dismutase, ascorbate peroxidase and glutathione cycling), growth and pigment pools. Changes in UVR sensitivity during photoacclimation were monitored by measuring UVR‐induced inhibition of carbon assimilation under standardized UV conditions using an indoor solar simulator. Both species showed immediate antioxidant responses due to their transfer to the outdoor conditions. Furthermore, upon outdoor exposure, carbon assimilation and growth rates were reduced in both species compared with initial conditions; however, these effects were most pronounced in D. tertiolecta. Outdoor UV exposure did not alter antioxidant levels when compared with PAR‐only controls in both species. In contrast, growth was significantly affected in the static UVR cultures, concurrent with significantly enhanced UVR resistance. We conclude that antioxidants play a minor role in the reinforcement of natural UVR resistance in T. weissflogii and D. tertiolecta. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/94740 Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; et al.; Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 85; 6; 11-2009; 1336-1345 0031-8655 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/94740 |
identifier_str_mv |
Janknegt, Paul J.; De Graaff, C. Marco; Van De Poll, Willem H.; Visser, Ronald J. W.; Helbling, Eduardo Walter; et al.; Antioxidative Responses of Two Marine Microalgae During Acclimation to Static and Fluctuating Natural UV Radiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 85; 6; 11-2009; 1336-1345 0031-8655 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-1097.2009.00603.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2009.00603.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270022588694528 |
score |
13.13397 |