Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
- Autores
- La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.
Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos
Fil: Feng, Bin. University of Pittsburgh; Estados Unidos
Fil: Schwartz, Erica S.. University of Pittsburgh; Estados Unidos
Fil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gebhart, G. F.. University of Pittsburgh; Estados Unidos - Materia
-
Hypertonicity
Colorectum
visceral
hypersensitivity - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/197745
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Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivityLa, Jun HoFeng, BinSchwartz, Erica S.Brumovsky, Pablo RodolfoGebhart, G. F.HypertonicityColorectumvisceralhypersensitivityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.Fil: La, Jun Ho. University of Pittsburgh; Estados UnidosFil: Feng, Bin. University of Pittsburgh; Estados UnidosFil: Schwartz, Erica S.. University of Pittsburgh; Estados UnidosFil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gebhart, G. F.. University of Pittsburgh; Estados UnidosAmerican Physiological Society2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197745La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.; Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 303; 7; 10-2012; 802-8090193-1857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpgi.00259.2012info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpgi.00259.2012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:07Zoai:ri.conicet.gov.ar:11336/197745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:08.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
title |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
spellingShingle |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity La, Jun Ho Hypertonicity Colorectum visceral hypersensitivity |
title_short |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
title_full |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
title_fullStr |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
title_full_unstemmed |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
title_sort |
Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity |
dc.creator.none.fl_str_mv |
La, Jun Ho Feng, Bin Schwartz, Erica S. Brumovsky, Pablo Rodolfo Gebhart, G. F. |
author |
La, Jun Ho |
author_facet |
La, Jun Ho Feng, Bin Schwartz, Erica S. Brumovsky, Pablo Rodolfo Gebhart, G. F. |
author_role |
author |
author2 |
Feng, Bin Schwartz, Erica S. Brumovsky, Pablo Rodolfo Gebhart, G. F. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Hypertonicity Colorectum visceral hypersensitivity |
topic |
Hypertonicity Colorectum visceral hypersensitivity |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain. Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos Fil: Feng, Bin. University of Pittsburgh; Estados Unidos Fil: Schwartz, Erica S.. University of Pittsburgh; Estados Unidos Fil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gebhart, G. F.. University of Pittsburgh; Estados Unidos |
description |
Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/197745 La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.; Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 303; 7; 10-2012; 802-809 0193-1857 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/197745 |
identifier_str_mv |
La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.; Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 303; 7; 10-2012; 802-809 0193-1857 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpgi.00259.2012 info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpgi.00259.2012 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Physiological Society |
publisher.none.fl_str_mv |
American Physiological Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613496976703488 |
score |
13.070432 |