Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity

Autores
La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.
Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos
Fil: Feng, Bin. University of Pittsburgh; Estados Unidos
Fil: Schwartz, Erica S.. University of Pittsburgh; Estados Unidos
Fil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gebhart, G. F.. University of Pittsburgh; Estados Unidos
Materia
Hypertonicity
Colorectum
visceral
hypersensitivity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197745

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivityLa, Jun HoFeng, BinSchwartz, Erica S.Brumovsky, Pablo RodolfoGebhart, G. F.HypertonicityColorectumvisceralhypersensitivityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.Fil: La, Jun Ho. University of Pittsburgh; Estados UnidosFil: Feng, Bin. University of Pittsburgh; Estados UnidosFil: Schwartz, Erica S.. University of Pittsburgh; Estados UnidosFil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gebhart, G. F.. University of Pittsburgh; Estados UnidosAmerican Physiological Society2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197745La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.; Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 303; 7; 10-2012; 802-8090193-1857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpgi.00259.2012info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpgi.00259.2012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:07Zoai:ri.conicet.gov.ar:11336/197745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:08.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
title Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
spellingShingle Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
La, Jun Ho
Hypertonicity
Colorectum
visceral
hypersensitivity
title_short Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
title_full Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
title_fullStr Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
title_full_unstemmed Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
title_sort Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity
dc.creator.none.fl_str_mv La, Jun Ho
Feng, Bin
Schwartz, Erica S.
Brumovsky, Pablo Rodolfo
Gebhart, G. F.
author La, Jun Ho
author_facet La, Jun Ho
Feng, Bin
Schwartz, Erica S.
Brumovsky, Pablo Rodolfo
Gebhart, G. F.
author_role author
author2 Feng, Bin
Schwartz, Erica S.
Brumovsky, Pablo Rodolfo
Gebhart, G. F.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hypertonicity
Colorectum
visceral
hypersensitivity
topic Hypertonicity
Colorectum
visceral
hypersensitivity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.
Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos
Fil: Feng, Bin. University of Pittsburgh; Estados Unidos
Fil: Schwartz, Erica S.. University of Pittsburgh; Estados Unidos
Fil: Brumovsky, Pablo Rodolfo. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gebhart, G. F.. University of Pittsburgh; Estados Unidos
description Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197745
La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.; Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 303; 7; 10-2012; 802-809
0193-1857
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197745
identifier_str_mv La, Jun Ho; Feng, Bin; Schwartz, Erica S.; Brumovsky, Pablo Rodolfo; Gebhart, G. F.; Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 303; 7; 10-2012; 802-809
0193-1857
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpgi.00259.2012
info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpgi.00259.2012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432