GABA receptors in Caenrohabditis elegans embryonic muscle cells

Autores
Hernando, Guillermina Silvana; Bouzat, Cecilia Beatriz
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
GABA receptors mediate fast inhibitory neurotransmission in both vertebrates and invertebrates. They are targets of anxiolytic and antiepileptic drugs as well as of commonly used insecticides and antihelminthics. Here we investigated GABA receptors in the free-living nematode Caenorhabditis elegans, which is a model for the study of the nervous system as well as a model of parasitic nematodes. Nematode muscle contains, in addition to two types of nicotinic receptors, a GABA receptor. We used a primary culture to explore the functional properties of GABA receptors from L1 muscle cells at the macroscopic and single-channel levels. Rapid application of GABA to L1 muscle cells elicits inward currents that decay fast due to desensitization. In cell-attached patches, 1-10mM GABA activates channels of ~ 2.6 pA (membrane potential: +100mV) and a mean duration of about 0.22 ms. The frequency of channel openings increases as a function of GABA concentration. The analysis of single-channel properties shows a homogenous channel population, indicating the presence of a single GABA receptor subtype. Our study contributes to the understanding of muscle GABA receptor properties and, in turn, provides new avenues for exploration of anthelmintic therapies
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Argentina
Sociedad Argentina de Investigación en Neurociencia
Materia
GABA RECEPTORS
EMBRYONIC MUSCLE CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242426
Acceder
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network_name_str CONICET Digital (CONICET)
spelling GABA receptors in Caenrohabditis elegans embryonic muscle cellsHernando, Guillermina SilvanaBouzat, Cecilia BeatrizGABA RECEPTORSEMBRYONIC MUSCLE CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1GABA receptors mediate fast inhibitory neurotransmission in both vertebrates and invertebrates. They are targets of anxiolytic and antiepileptic drugs as well as of commonly used insecticides and antihelminthics. Here we investigated GABA receptors in the free-living nematode Caenorhabditis elegans, which is a model for the study of the nervous system as well as a model of parasitic nematodes. Nematode muscle contains, in addition to two types of nicotinic receptors, a GABA receptor. We used a primary culture to explore the functional properties of GABA receptors from L1 muscle cells at the macroscopic and single-channel levels. Rapid application of GABA to L1 muscle cells elicits inward currents that decay fast due to desensitization. In cell-attached patches, 1-10mM GABA activates channels of ~ 2.6 pA (membrane potential: +100mV) and a mean duration of about 0.22 ms. The frequency of channel openings increases as a function of GABA concentration. The analysis of single-channel properties shows a homogenous channel population, indicating the presence of a single GABA receptor subtype. Our study contributes to the understanding of muscle GABA receptor properties and, in turn, provides new avenues for exploration of anthelmintic therapiesFil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXXVI Congreso Anual de la Sociedad Argentina de Investigación en NeurocienciaArgentinaSociedad Argentina de Investigación en NeurocienciaSociedad Argentina de Investigación en Neurociencia2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242426GABA receptors in Caenrohabditis elegans embryonic muscle cells; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Argentina; 2011; 231-232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:12Zoai:ri.conicet.gov.ar:11336/242426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:12.688CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GABA receptors in Caenrohabditis elegans embryonic muscle cells
title GABA receptors in Caenrohabditis elegans embryonic muscle cells
spellingShingle GABA receptors in Caenrohabditis elegans embryonic muscle cells
Hernando, Guillermina Silvana
GABA RECEPTORS
EMBRYONIC MUSCLE CELLS
title_short GABA receptors in Caenrohabditis elegans embryonic muscle cells
title_full GABA receptors in Caenrohabditis elegans embryonic muscle cells
title_fullStr GABA receptors in Caenrohabditis elegans embryonic muscle cells
title_full_unstemmed GABA receptors in Caenrohabditis elegans embryonic muscle cells
title_sort GABA receptors in Caenrohabditis elegans embryonic muscle cells
dc.creator.none.fl_str_mv Hernando, Guillermina Silvana
Bouzat, Cecilia Beatriz
author Hernando, Guillermina Silvana
author_facet Hernando, Guillermina Silvana
Bouzat, Cecilia Beatriz
author_role author
author2 Bouzat, Cecilia Beatriz
author2_role author
dc.subject.none.fl_str_mv GABA RECEPTORS
EMBRYONIC MUSCLE CELLS
topic GABA RECEPTORS
EMBRYONIC MUSCLE CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv GABA receptors mediate fast inhibitory neurotransmission in both vertebrates and invertebrates. They are targets of anxiolytic and antiepileptic drugs as well as of commonly used insecticides and antihelminthics. Here we investigated GABA receptors in the free-living nematode Caenorhabditis elegans, which is a model for the study of the nervous system as well as a model of parasitic nematodes. Nematode muscle contains, in addition to two types of nicotinic receptors, a GABA receptor. We used a primary culture to explore the functional properties of GABA receptors from L1 muscle cells at the macroscopic and single-channel levels. Rapid application of GABA to L1 muscle cells elicits inward currents that decay fast due to desensitization. In cell-attached patches, 1-10mM GABA activates channels of ~ 2.6 pA (membrane potential: +100mV) and a mean duration of about 0.22 ms. The frequency of channel openings increases as a function of GABA concentration. The analysis of single-channel properties shows a homogenous channel population, indicating the presence of a single GABA receptor subtype. Our study contributes to the understanding of muscle GABA receptor properties and, in turn, provides new avenues for exploration of anthelmintic therapies
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Argentina
Sociedad Argentina de Investigación en Neurociencia
description GABA receptors mediate fast inhibitory neurotransmission in both vertebrates and invertebrates. They are targets of anxiolytic and antiepileptic drugs as well as of commonly used insecticides and antihelminthics. Here we investigated GABA receptors in the free-living nematode Caenorhabditis elegans, which is a model for the study of the nervous system as well as a model of parasitic nematodes. Nematode muscle contains, in addition to two types of nicotinic receptors, a GABA receptor. We used a primary culture to explore the functional properties of GABA receptors from L1 muscle cells at the macroscopic and single-channel levels. Rapid application of GABA to L1 muscle cells elicits inward currents that decay fast due to desensitization. In cell-attached patches, 1-10mM GABA activates channels of ~ 2.6 pA (membrane potential: +100mV) and a mean duration of about 0.22 ms. The frequency of channel openings increases as a function of GABA concentration. The analysis of single-channel properties shows a homogenous channel population, indicating the presence of a single GABA receptor subtype. Our study contributes to the understanding of muscle GABA receptor properties and, in turn, provides new avenues for exploration of anthelmintic therapies
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242426
GABA receptors in Caenrohabditis elegans embryonic muscle cells; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Argentina; 2011; 231-232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242426
identifier_str_mv GABA receptors in Caenrohabditis elegans embryonic muscle cells; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Argentina; 2011; 231-232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencia
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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