Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506

Autores
Suzuki, Masahiko; Baillo, Ayelen Antonella; Albarracín, Leonardo Miguel; Elean, Mariano Daniel; Serda, Rodrigo Exequiel; Suda, Yoshihito; Namai, Fu; Nishiyama, Keita; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506 can increase resistance of mice to the infections induced by Gram negative pathogens. In this work, we aimed to advance in the characterization of the immunomodulatory properties of the CRL1505 and CRL1506 strains by evaluating their effect on the Toll-like receptor 4 (TLR4)-triggered immune response in macrophages. We performed experiments using the murine macrophage cell line RAW 264.7 stimulated with lipopolysaccharide (LPS) to evaluate the transcriptomic changes induced by lactobacilli. These in vitro experiments were complemented with in vivo studies in mice. The CRL1505 and CRL1506 strains were administered to animals, Peyer´s patches and peritoneal macrophages were isolated, and ex vivo stimulated with LPS. Microarray transcriptomic studies and qPCR confirmation showed that CRL1505- and CRL1506-treated RAW macrophages responded to LPS stimulation with higher il6, il15, ifnβ, ifnγ, csf3, and selp expression and lower il1β, il12, ccl2, ccl8, and icam1 expression compared to control macrophages. In addition, Peyer´s patches and peritoneal macrophages from mice treated with lactobacilli produced higher levels of TNF-α, IFN-γ, IL-6, and CSF3 and lower IL-1β, IL-12, CSF2, CCL2 and CCL8 after LPS stimulation. Of note, lactobacilli also modulate regulatory factors in macrophages. L. plantarum CRL1506 increased il10 and socs2 while L. rhamnosus CRL1505 upregulated il27, socs1 and socs3 in RAW cells. Both lactobacilli equally modulated socs1 and socs2 expressions and IL-10 and IL-27 production in Peyer´s patches macrophages and socs3 and IL-10 in peritoneal cells. The results show that L. rhamnosus CRL1505 and L. plantarum CRL1506 can modulate different inflammatory mediators, adhesion molecules and regulatory factors in macrophages after TLR4 activation. This effect would allow an improved protection against pathogens together with minimal inflammatory-mediated tissue damage as shown in our previous in vivo studies with Gram negative bacterial infections.
Fil: Suzuki, Masahiko. Tohoku University; Japón
Fil: Baillo, Ayelen Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Serda, Rodrigo Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
PROBIOTICS
MACROPHAGES
TLR4
L. RHAMNOSUS CRL1505
L. PLANTARUM CRL1506
TRANSCRIPTOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281147
Acceder
id CONICETDig_ceee4766e23d34be662bbf21c05d6971
oai_identifier_str oai:ri.conicet.gov.ar:11336/281147
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506Suzuki, MasahikoBaillo, Ayelen AntonellaAlbarracín, Leonardo MiguelElean, Mariano DanielSerda, Rodrigo ExequielSuda, YoshihitoNamai, FuNishiyama, KeitaKitazawa, HarukiVillena, Julio CesarPROBIOTICSMACROPHAGESTLR4L. RHAMNOSUS CRL1505L. PLANTARUM CRL1506TRANSCRIPTOMICShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506 can increase resistance of mice to the infections induced by Gram negative pathogens. In this work, we aimed to advance in the characterization of the immunomodulatory properties of the CRL1505 and CRL1506 strains by evaluating their effect on the Toll-like receptor 4 (TLR4)-triggered immune response in macrophages. We performed experiments using the murine macrophage cell line RAW 264.7 stimulated with lipopolysaccharide (LPS) to evaluate the transcriptomic changes induced by lactobacilli. These in vitro experiments were complemented with in vivo studies in mice. The CRL1505 and CRL1506 strains were administered to animals, Peyer´s patches and peritoneal macrophages were isolated, and ex vivo stimulated with LPS. Microarray transcriptomic studies and qPCR confirmation showed that CRL1505- and CRL1506-treated RAW macrophages responded to LPS stimulation with higher il6, il15, ifnβ, ifnγ, csf3, and selp expression and lower il1β, il12, ccl2, ccl8, and icam1 expression compared to control macrophages. In addition, Peyer´s patches and peritoneal macrophages from mice treated with lactobacilli produced higher levels of TNF-α, IFN-γ, IL-6, and CSF3 and lower IL-1β, IL-12, CSF2, CCL2 and CCL8 after LPS stimulation. Of note, lactobacilli also modulate regulatory factors in macrophages. L. plantarum CRL1506 increased il10 and socs2 while L. rhamnosus CRL1505 upregulated il27, socs1 and socs3 in RAW cells. Both lactobacilli equally modulated socs1 and socs2 expressions and IL-10 and IL-27 production in Peyer´s patches macrophages and socs3 and IL-10 in peritoneal cells. The results show that L. rhamnosus CRL1505 and L. plantarum CRL1506 can modulate different inflammatory mediators, adhesion molecules and regulatory factors in macrophages after TLR4 activation. This effect would allow an improved protection against pathogens together with minimal inflammatory-mediated tissue damage as shown in our previous in vivo studies with Gram negative bacterial infections.Fil: Suzuki, Masahiko. Tohoku University; JapónFil: Baillo, Ayelen Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Serda, Rodrigo Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Suda, Yoshihito. Miyagi University; JapónFil: Namai, Fu. Tohoku University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMultidisciplinary Digital Publishing Institute2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/281147Suzuki, Masahiko; Baillo, Ayelen Antonella; Albarracín, Leonardo Miguel; Elean, Mariano Daniel; Serda, Rodrigo Exequiel; et al.; Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 26; 6; 2-2025; 1-261422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/6/2688info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26062688info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:22:48Zoai:ri.conicet.gov.ar:11336/281147instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:22:48.483CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
title Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
spellingShingle Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
Suzuki, Masahiko
PROBIOTICS
MACROPHAGES
TLR4
L. RHAMNOSUS CRL1505
L. PLANTARUM CRL1506
TRANSCRIPTOMICS
title_short Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
title_full Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
title_fullStr Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
title_full_unstemmed Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
title_sort Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506
dc.creator.none.fl_str_mv Suzuki, Masahiko
Baillo, Ayelen Antonella
Albarracín, Leonardo Miguel
Elean, Mariano Daniel
Serda, Rodrigo Exequiel
Suda, Yoshihito
Namai, Fu
Nishiyama, Keita
Kitazawa, Haruki
Villena, Julio Cesar
author Suzuki, Masahiko
author_facet Suzuki, Masahiko
Baillo, Ayelen Antonella
Albarracín, Leonardo Miguel
Elean, Mariano Daniel
Serda, Rodrigo Exequiel
Suda, Yoshihito
Namai, Fu
Nishiyama, Keita
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Baillo, Ayelen Antonella
Albarracín, Leonardo Miguel
Elean, Mariano Daniel
Serda, Rodrigo Exequiel
Suda, Yoshihito
Namai, Fu
Nishiyama, Keita
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROBIOTICS
MACROPHAGES
TLR4
L. RHAMNOSUS CRL1505
L. PLANTARUM CRL1506
TRANSCRIPTOMICS
topic PROBIOTICS
MACROPHAGES
TLR4
L. RHAMNOSUS CRL1505
L. PLANTARUM CRL1506
TRANSCRIPTOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506 can increase resistance of mice to the infections induced by Gram negative pathogens. In this work, we aimed to advance in the characterization of the immunomodulatory properties of the CRL1505 and CRL1506 strains by evaluating their effect on the Toll-like receptor 4 (TLR4)-triggered immune response in macrophages. We performed experiments using the murine macrophage cell line RAW 264.7 stimulated with lipopolysaccharide (LPS) to evaluate the transcriptomic changes induced by lactobacilli. These in vitro experiments were complemented with in vivo studies in mice. The CRL1505 and CRL1506 strains were administered to animals, Peyer´s patches and peritoneal macrophages were isolated, and ex vivo stimulated with LPS. Microarray transcriptomic studies and qPCR confirmation showed that CRL1505- and CRL1506-treated RAW macrophages responded to LPS stimulation with higher il6, il15, ifnβ, ifnγ, csf3, and selp expression and lower il1β, il12, ccl2, ccl8, and icam1 expression compared to control macrophages. In addition, Peyer´s patches and peritoneal macrophages from mice treated with lactobacilli produced higher levels of TNF-α, IFN-γ, IL-6, and CSF3 and lower IL-1β, IL-12, CSF2, CCL2 and CCL8 after LPS stimulation. Of note, lactobacilli also modulate regulatory factors in macrophages. L. plantarum CRL1506 increased il10 and socs2 while L. rhamnosus CRL1505 upregulated il27, socs1 and socs3 in RAW cells. Both lactobacilli equally modulated socs1 and socs2 expressions and IL-10 and IL-27 production in Peyer´s patches macrophages and socs3 and IL-10 in peritoneal cells. The results show that L. rhamnosus CRL1505 and L. plantarum CRL1506 can modulate different inflammatory mediators, adhesion molecules and regulatory factors in macrophages after TLR4 activation. This effect would allow an improved protection against pathogens together with minimal inflammatory-mediated tissue damage as shown in our previous in vivo studies with Gram negative bacterial infections.
Fil: Suzuki, Masahiko. Tohoku University; Japón
Fil: Baillo, Ayelen Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Serda, Rodrigo Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506 can increase resistance of mice to the infections induced by Gram negative pathogens. In this work, we aimed to advance in the characterization of the immunomodulatory properties of the CRL1505 and CRL1506 strains by evaluating their effect on the Toll-like receptor 4 (TLR4)-triggered immune response in macrophages. We performed experiments using the murine macrophage cell line RAW 264.7 stimulated with lipopolysaccharide (LPS) to evaluate the transcriptomic changes induced by lactobacilli. These in vitro experiments were complemented with in vivo studies in mice. The CRL1505 and CRL1506 strains were administered to animals, Peyer´s patches and peritoneal macrophages were isolated, and ex vivo stimulated with LPS. Microarray transcriptomic studies and qPCR confirmation showed that CRL1505- and CRL1506-treated RAW macrophages responded to LPS stimulation with higher il6, il15, ifnβ, ifnγ, csf3, and selp expression and lower il1β, il12, ccl2, ccl8, and icam1 expression compared to control macrophages. In addition, Peyer´s patches and peritoneal macrophages from mice treated with lactobacilli produced higher levels of TNF-α, IFN-γ, IL-6, and CSF3 and lower IL-1β, IL-12, CSF2, CCL2 and CCL8 after LPS stimulation. Of note, lactobacilli also modulate regulatory factors in macrophages. L. plantarum CRL1506 increased il10 and socs2 while L. rhamnosus CRL1505 upregulated il27, socs1 and socs3 in RAW cells. Both lactobacilli equally modulated socs1 and socs2 expressions and IL-10 and IL-27 production in Peyer´s patches macrophages and socs3 and IL-10 in peritoneal cells. The results show that L. rhamnosus CRL1505 and L. plantarum CRL1506 can modulate different inflammatory mediators, adhesion molecules and regulatory factors in macrophages after TLR4 activation. This effect would allow an improved protection against pathogens together with minimal inflammatory-mediated tissue damage as shown in our previous in vivo studies with Gram negative bacterial infections.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281147
Suzuki, Masahiko; Baillo, Ayelen Antonella; Albarracín, Leonardo Miguel; Elean, Mariano Daniel; Serda, Rodrigo Exequiel; et al.; Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 26; 6; 2-2025; 1-26
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281147
identifier_str_mv Suzuki, Masahiko; Baillo, Ayelen Antonella; Albarracín, Leonardo Miguel; Elean, Mariano Daniel; Serda, Rodrigo Exequiel; et al.; Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 26; 6; 2-2025; 1-26
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/6/2688
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26062688
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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