Cell wall modification of the green algae Scenedesmus sp.

Autores
Velázquez, María Belén; Gomez Casati, Diego Fabian; Barchiesi, Julieta; Busi, María Victoria
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Scenedesmus is one of the most common freshwater algae genera. It can exist as unicellular organism; however, it is also found in coenobia organization which consists of four or eight cells inside a parental mother wall. The structure of the cell wall is important because it not only shapes the cell, but it is also the structure that is in contact with the external environment. In particular, Scenedesmus has a three-layered cell wall with cellulose, algaenan and pectin. In this work we generated transgenic algae overexpressing carbohydrate binding modules in the cell wall. The transgenic lines obtained are larger than wild type, their surface-to-volume ratio is affected, and the large mass promotes sinking. Cell wall components are altered, specifically the pectin layer, which increases the union between cells forming a conglomerate. We observed a greater laxity of the cell wall and an increase in the amount of pectin, fact that agree with the phenotype of transgenic plants that overexpressed starch binding domains of Arabidopsis thaliana starch synthase III. Apart from lack of an efficient harvesting technology, one of the major difficulties in using algae is their recalcitrant cell wall, which, on the other hand, is considered as an excellent food supplement. The presence of sugars in the cell wall following hydrolysis facilitates bioethanol production. Our results suggest that transgenic Scenedesmus have an advantage for the production of biofuels in terms of better access to the inner cellulose layer and facilitate its degradation and saccharification.
Fil: Velázquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Barchiesi, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
CELL WALL
SCENEDESMUS
ALGAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/204440
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/204440
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell wall modification of the green algae Scenedesmus sp.Velázquez, María BelénGomez Casati, Diego FabianBarchiesi, JulietaBusi, María VictoriaCELL WALLSCENEDESMUSALGAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Scenedesmus is one of the most common freshwater algae genera. It can exist as unicellular organism; however, it is also found in coenobia organization which consists of four or eight cells inside a parental mother wall. The structure of the cell wall is important because it not only shapes the cell, but it is also the structure that is in contact with the external environment. In particular, Scenedesmus has a three-layered cell wall with cellulose, algaenan and pectin. In this work we generated transgenic algae overexpressing carbohydrate binding modules in the cell wall. The transgenic lines obtained are larger than wild type, their surface-to-volume ratio is affected, and the large mass promotes sinking. Cell wall components are altered, specifically the pectin layer, which increases the union between cells forming a conglomerate. We observed a greater laxity of the cell wall and an increase in the amount of pectin, fact that agree with the phenotype of transgenic plants that overexpressed starch binding domains of Arabidopsis thaliana starch synthase III. Apart from lack of an efficient harvesting technology, one of the major difficulties in using algae is their recalcitrant cell wall, which, on the other hand, is considered as an excellent food supplement. The presence of sugars in the cell wall following hydrolysis facilitates bioethanol production. Our results suggest that transgenic Scenedesmus have an advantage for the production of biofuels in terms of better access to the inner cellulose layer and facilitate its degradation and saccharification.Fil: Velázquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Barchiesi, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General MicrobiologyCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/204440Cell wall modification of the green algae Scenedesmus sp.; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 149-1490327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2020-version-final.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:38Zoai:ri.conicet.gov.ar:11336/204440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:38.61CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell wall modification of the green algae Scenedesmus sp.
title Cell wall modification of the green algae Scenedesmus sp.
spellingShingle Cell wall modification of the green algae Scenedesmus sp.
Velázquez, María Belén
CELL WALL
SCENEDESMUS
ALGAE
title_short Cell wall modification of the green algae Scenedesmus sp.
title_full Cell wall modification of the green algae Scenedesmus sp.
title_fullStr Cell wall modification of the green algae Scenedesmus sp.
title_full_unstemmed Cell wall modification of the green algae Scenedesmus sp.
title_sort Cell wall modification of the green algae Scenedesmus sp.
dc.creator.none.fl_str_mv Velázquez, María Belén
Gomez Casati, Diego Fabian
Barchiesi, Julieta
Busi, María Victoria
author Velázquez, María Belén
author_facet Velázquez, María Belén
Gomez Casati, Diego Fabian
Barchiesi, Julieta
Busi, María Victoria
author_role author
author2 Gomez Casati, Diego Fabian
Barchiesi, Julieta
Busi, María Victoria
author2_role author
author
author
dc.subject.none.fl_str_mv CELL WALL
SCENEDESMUS
ALGAE
topic CELL WALL
SCENEDESMUS
ALGAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Scenedesmus is one of the most common freshwater algae genera. It can exist as unicellular organism; however, it is also found in coenobia organization which consists of four or eight cells inside a parental mother wall. The structure of the cell wall is important because it not only shapes the cell, but it is also the structure that is in contact with the external environment. In particular, Scenedesmus has a three-layered cell wall with cellulose, algaenan and pectin. In this work we generated transgenic algae overexpressing carbohydrate binding modules in the cell wall. The transgenic lines obtained are larger than wild type, their surface-to-volume ratio is affected, and the large mass promotes sinking. Cell wall components are altered, specifically the pectin layer, which increases the union between cells forming a conglomerate. We observed a greater laxity of the cell wall and an increase in the amount of pectin, fact that agree with the phenotype of transgenic plants that overexpressed starch binding domains of Arabidopsis thaliana starch synthase III. Apart from lack of an efficient harvesting technology, one of the major difficulties in using algae is their recalcitrant cell wall, which, on the other hand, is considered as an excellent food supplement. The presence of sugars in the cell wall following hydrolysis facilitates bioethanol production. Our results suggest that transgenic Scenedesmus have an advantage for the production of biofuels in terms of better access to the inner cellulose layer and facilitate its degradation and saccharification.
Fil: Velázquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Barchiesi, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description Scenedesmus is one of the most common freshwater algae genera. It can exist as unicellular organism; however, it is also found in coenobia organization which consists of four or eight cells inside a parental mother wall. The structure of the cell wall is important because it not only shapes the cell, but it is also the structure that is in contact with the external environment. In particular, Scenedesmus has a three-layered cell wall with cellulose, algaenan and pectin. In this work we generated transgenic algae overexpressing carbohydrate binding modules in the cell wall. The transgenic lines obtained are larger than wild type, their surface-to-volume ratio is affected, and the large mass promotes sinking. Cell wall components are altered, specifically the pectin layer, which increases the union between cells forming a conglomerate. We observed a greater laxity of the cell wall and an increase in the amount of pectin, fact that agree with the phenotype of transgenic plants that overexpressed starch binding domains of Arabidopsis thaliana starch synthase III. Apart from lack of an efficient harvesting technology, one of the major difficulties in using algae is their recalcitrant cell wall, which, on the other hand, is considered as an excellent food supplement. The presence of sugars in the cell wall following hydrolysis facilitates bioethanol production. Our results suggest that transgenic Scenedesmus have an advantage for the production of biofuels in terms of better access to the inner cellulose layer and facilitate its degradation and saccharification.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/204440
Cell wall modification of the green algae Scenedesmus sp.; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 149-149
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/204440
identifier_str_mv Cell wall modification of the green algae Scenedesmus sp.; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 149-149
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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