Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes

Autores
Contreras, Ely; Bhoi, Jacob D.; Sonoda, Takuma; Birnbaumer, Lutz; Schmidt, Tiffany M.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Melanopsin signaling within intrinsically photosensitive retinal ganglion cell (ipRGC) subtypes impacts a broad range of behaviors from circadian photoentrainment to conscious visual perception. Yet, how melanopsin phototransduction within M1-M6 ipRGC subtypes impacts cellular signaling to drive diverse behaviors is still largely unresolved. The identity of the phototransduction channels in each subtype is key to understanding this central question but has remained controversial. In this study, we resolve two opposing models of M4 phototransduction, demonstrating that hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are dispensable for this process and providing support for a pathway involving melanopsin-dependent potassium channel closure and canonical transient receptor potential (TRPC) channel opening. Surprisingly, we find that HCN channels are likewise dispensable for M2 phototransduction, contradicting the current model. We instead show that M2 phototransduction requires TRPC channels in conjunction with T-type voltage-gated calcium channels, identifying a novel melanopsin phototransduction target. Collectively, this work resolves key discrepancies in our understanding of ipRGC phototransduction pathways in multiple subtypes and adds to mounting evidence that ipRGC subtypes employ diverse phototransduction cascades to fine-tune cellular responses for downstream behaviors.
Fil: Contreras, Ely. Northwestern University; Estados Unidos
Fil: Bhoi, Jacob D.. Northwestern University; Estados Unidos
Fil: Sonoda, Takuma. Northwestern University; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Schmidt, Tiffany M.. Northwestern University; Estados Unidos
Materia
ipRGC
MELANOPSIN
TRP CHANNEL
GANGLION CELL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/256337
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/256337
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypesContreras, ElyBhoi, Jacob D.Sonoda, TakumaBirnbaumer, LutzSchmidt, Tiffany M.ipRGCMELANOPSINTRP CHANNELGANGLION CELLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Melanopsin signaling within intrinsically photosensitive retinal ganglion cell (ipRGC) subtypes impacts a broad range of behaviors from circadian photoentrainment to conscious visual perception. Yet, how melanopsin phototransduction within M1-M6 ipRGC subtypes impacts cellular signaling to drive diverse behaviors is still largely unresolved. The identity of the phototransduction channels in each subtype is key to understanding this central question but has remained controversial. In this study, we resolve two opposing models of M4 phototransduction, demonstrating that hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are dispensable for this process and providing support for a pathway involving melanopsin-dependent potassium channel closure and canonical transient receptor potential (TRPC) channel opening. Surprisingly, we find that HCN channels are likewise dispensable for M2 phototransduction, contradicting the current model. We instead show that M2 phototransduction requires TRPC channels in conjunction with T-type voltage-gated calcium channels, identifying a novel melanopsin phototransduction target. Collectively, this work resolves key discrepancies in our understanding of ipRGC phototransduction pathways in multiple subtypes and adds to mounting evidence that ipRGC subtypes employ diverse phototransduction cascades to fine-tune cellular responses for downstream behaviors.Fil: Contreras, Ely. Northwestern University; Estados UnidosFil: Bhoi, Jacob D.. Northwestern University; Estados UnidosFil: Sonoda, Takuma. Northwestern University; Estados UnidosFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Schmidt, Tiffany M.. Northwestern University; Estados UnidoseLife Sciences Publications2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256337Contreras, Ely; Bhoi, Jacob D.; Sonoda, Takuma; Birnbaumer, Lutz; Schmidt, Tiffany M.; Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes; eLife Sciences Publications; eLife; 12; 11-2023; 1-302050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/80749info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.80749info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:13:34Zoai:ri.conicet.gov.ar:11336/256337instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:13:34.693CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
title Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
spellingShingle Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
Contreras, Ely
ipRGC
MELANOPSIN
TRP CHANNEL
GANGLION CELL
title_short Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
title_full Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
title_fullStr Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
title_full_unstemmed Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
title_sort Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes
dc.creator.none.fl_str_mv Contreras, Ely
Bhoi, Jacob D.
Sonoda, Takuma
Birnbaumer, Lutz
Schmidt, Tiffany M.
author Contreras, Ely
author_facet Contreras, Ely
Bhoi, Jacob D.
Sonoda, Takuma
Birnbaumer, Lutz
Schmidt, Tiffany M.
author_role author
author2 Bhoi, Jacob D.
Sonoda, Takuma
Birnbaumer, Lutz
Schmidt, Tiffany M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv ipRGC
MELANOPSIN
TRP CHANNEL
GANGLION CELL
topic ipRGC
MELANOPSIN
TRP CHANNEL
GANGLION CELL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Melanopsin signaling within intrinsically photosensitive retinal ganglion cell (ipRGC) subtypes impacts a broad range of behaviors from circadian photoentrainment to conscious visual perception. Yet, how melanopsin phototransduction within M1-M6 ipRGC subtypes impacts cellular signaling to drive diverse behaviors is still largely unresolved. The identity of the phototransduction channels in each subtype is key to understanding this central question but has remained controversial. In this study, we resolve two opposing models of M4 phototransduction, demonstrating that hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are dispensable for this process and providing support for a pathway involving melanopsin-dependent potassium channel closure and canonical transient receptor potential (TRPC) channel opening. Surprisingly, we find that HCN channels are likewise dispensable for M2 phototransduction, contradicting the current model. We instead show that M2 phototransduction requires TRPC channels in conjunction with T-type voltage-gated calcium channels, identifying a novel melanopsin phototransduction target. Collectively, this work resolves key discrepancies in our understanding of ipRGC phototransduction pathways in multiple subtypes and adds to mounting evidence that ipRGC subtypes employ diverse phototransduction cascades to fine-tune cellular responses for downstream behaviors.
Fil: Contreras, Ely. Northwestern University; Estados Unidos
Fil: Bhoi, Jacob D.. Northwestern University; Estados Unidos
Fil: Sonoda, Takuma. Northwestern University; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Schmidt, Tiffany M.. Northwestern University; Estados Unidos
description Melanopsin signaling within intrinsically photosensitive retinal ganglion cell (ipRGC) subtypes impacts a broad range of behaviors from circadian photoentrainment to conscious visual perception. Yet, how melanopsin phototransduction within M1-M6 ipRGC subtypes impacts cellular signaling to drive diverse behaviors is still largely unresolved. The identity of the phototransduction channels in each subtype is key to understanding this central question but has remained controversial. In this study, we resolve two opposing models of M4 phototransduction, demonstrating that hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are dispensable for this process and providing support for a pathway involving melanopsin-dependent potassium channel closure and canonical transient receptor potential (TRPC) channel opening. Surprisingly, we find that HCN channels are likewise dispensable for M2 phototransduction, contradicting the current model. We instead show that M2 phototransduction requires TRPC channels in conjunction with T-type voltage-gated calcium channels, identifying a novel melanopsin phototransduction target. Collectively, this work resolves key discrepancies in our understanding of ipRGC phototransduction pathways in multiple subtypes and adds to mounting evidence that ipRGC subtypes employ diverse phototransduction cascades to fine-tune cellular responses for downstream behaviors.
publishDate 2023
dc.date.none.fl_str_mv 2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/256337
Contreras, Ely; Bhoi, Jacob D.; Sonoda, Takuma; Birnbaumer, Lutz; Schmidt, Tiffany M.; Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes; eLife Sciences Publications; eLife; 12; 11-2023; 1-30
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/256337
identifier_str_mv Contreras, Ely; Bhoi, Jacob D.; Sonoda, Takuma; Birnbaumer, Lutz; Schmidt, Tiffany M.; Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes; eLife Sciences Publications; eLife; 12; 11-2023; 1-30
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/80749
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.80749
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847425990470402048
score 13.10058

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