Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia

Autores
Arroyo, Daniela Soledad; Rodríguez, Cecilia Inés; Bussi, Claudio; Manzone Rodriguez, Clarisa; Sastre, Darío; Heller, Viviana; Stanganelli, Carmen Graciela; Slavutsky, Irma Rosa; Iribarren, Pablo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the western hemisphere. It is characterized by a clonal proliferation of a population of CD5+ B lymphocytes that accumulate in the secondary lymphoid tissues, bone marrow, and blood. Some CLL patients remain free of symptoms for decades, whereas others rapidly become symptomatic or develop high-risk disease. Studying autophagy, which may modulate key protein expression and cell survival, may be important to the search for novel prognostic factors and molecules. Here, we applied flow cytometry technology to simultaneously detect autophagy protein LC3B with classical phenotypical markers used for the identification of tumoral CLL B cell clones. We found that two patients with progressing CLL showed increased expression of the autophagy protein LC3B, in addition to positive expression of CD38 and ZAP70 and unmutated status of IGHV. Our data suggest that activation of autophagy flux may correlate with CLL progression even before Ibrutinib treatment.
Fil: Arroyo, Daniela Soledad. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodríguez, Cecilia Inés. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Bussi, Claudio. The Francis Crick Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manzone Rodriguez, Clarisa. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sastre, Darío. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Heller, Viviana. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Stanganelli, Carmen Graciela. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Iribarren, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
CHRONIC LYMPHOCYTIC LEUKEMIA
AUTOPHAGY
PROTEIN LC3
IGHV
CANCER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146356
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/146356
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemiaArroyo, Daniela SoledadRodríguez, Cecilia InésBussi, ClaudioManzone Rodriguez, ClarisaSastre, DaríoHeller, VivianaStanganelli, Carmen GracielaSlavutsky, Irma RosaIribarren, PabloCHRONIC LYMPHOCYTIC LEUKEMIAAUTOPHAGYPROTEIN LC3IGHVCANCERhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the western hemisphere. It is characterized by a clonal proliferation of a population of CD5+ B lymphocytes that accumulate in the secondary lymphoid tissues, bone marrow, and blood. Some CLL patients remain free of symptoms for decades, whereas others rapidly become symptomatic or develop high-risk disease. Studying autophagy, which may modulate key protein expression and cell survival, may be important to the search for novel prognostic factors and molecules. Here, we applied flow cytometry technology to simultaneously detect autophagy protein LC3B with classical phenotypical markers used for the identification of tumoral CLL B cell clones. We found that two patients with progressing CLL showed increased expression of the autophagy protein LC3B, in addition to positive expression of CD38 and ZAP70 and unmutated status of IGHV. Our data suggest that activation of autophagy flux may correlate with CLL progression even before Ibrutinib treatment.Fil: Arroyo, Daniela Soledad. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodríguez, Cecilia Inés. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaFil: Bussi, Claudio. The Francis Crick Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manzone Rodriguez, Clarisa. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Sastre, Darío. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Heller, Viviana. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Stanganelli, Carmen Graciela. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Iribarren, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFrontiers Media2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146356Arroyo, Daniela Soledad; Rodríguez, Cecilia Inés; Bussi, Claudio; Manzone Rodriguez, Clarisa; Sastre, Darío; et al.; Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia; Frontiers Media; Frontiers in Endocrinology; 11; 321; 7-2020; 1-61664-2392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2020.00321info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:27Zoai:ri.conicet.gov.ar:11336/146356instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:27.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
title Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
spellingShingle Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
Arroyo, Daniela Soledad
CHRONIC LYMPHOCYTIC LEUKEMIA
AUTOPHAGY
PROTEIN LC3
IGHV
CANCER
title_short Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
title_full Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
title_fullStr Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
title_full_unstemmed Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
title_sort Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia
dc.creator.none.fl_str_mv Arroyo, Daniela Soledad
Rodríguez, Cecilia Inés
Bussi, Claudio
Manzone Rodriguez, Clarisa
Sastre, Darío
Heller, Viviana
Stanganelli, Carmen Graciela
Slavutsky, Irma Rosa
Iribarren, Pablo
author Arroyo, Daniela Soledad
author_facet Arroyo, Daniela Soledad
Rodríguez, Cecilia Inés
Bussi, Claudio
Manzone Rodriguez, Clarisa
Sastre, Darío
Heller, Viviana
Stanganelli, Carmen Graciela
Slavutsky, Irma Rosa
Iribarren, Pablo
author_role author
author2 Rodríguez, Cecilia Inés
Bussi, Claudio
Manzone Rodriguez, Clarisa
Sastre, Darío
Heller, Viviana
Stanganelli, Carmen Graciela
Slavutsky, Irma Rosa
Iribarren, Pablo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHRONIC LYMPHOCYTIC LEUKEMIA
AUTOPHAGY
PROTEIN LC3
IGHV
CANCER
topic CHRONIC LYMPHOCYTIC LEUKEMIA
AUTOPHAGY
PROTEIN LC3
IGHV
CANCER
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the western hemisphere. It is characterized by a clonal proliferation of a population of CD5+ B lymphocytes that accumulate in the secondary lymphoid tissues, bone marrow, and blood. Some CLL patients remain free of symptoms for decades, whereas others rapidly become symptomatic or develop high-risk disease. Studying autophagy, which may modulate key protein expression and cell survival, may be important to the search for novel prognostic factors and molecules. Here, we applied flow cytometry technology to simultaneously detect autophagy protein LC3B with classical phenotypical markers used for the identification of tumoral CLL B cell clones. We found that two patients with progressing CLL showed increased expression of the autophagy protein LC3B, in addition to positive expression of CD38 and ZAP70 and unmutated status of IGHV. Our data suggest that activation of autophagy flux may correlate with CLL progression even before Ibrutinib treatment.
Fil: Arroyo, Daniela Soledad. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodríguez, Cecilia Inés. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Bussi, Claudio. The Francis Crick Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manzone Rodriguez, Clarisa. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sastre, Darío. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Heller, Viviana. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Stanganelli, Carmen Graciela. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Iribarren, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the western hemisphere. It is characterized by a clonal proliferation of a population of CD5+ B lymphocytes that accumulate in the secondary lymphoid tissues, bone marrow, and blood. Some CLL patients remain free of symptoms for decades, whereas others rapidly become symptomatic or develop high-risk disease. Studying autophagy, which may modulate key protein expression and cell survival, may be important to the search for novel prognostic factors and molecules. Here, we applied flow cytometry technology to simultaneously detect autophagy protein LC3B with classical phenotypical markers used for the identification of tumoral CLL B cell clones. We found that two patients with progressing CLL showed increased expression of the autophagy protein LC3B, in addition to positive expression of CD38 and ZAP70 and unmutated status of IGHV. Our data suggest that activation of autophagy flux may correlate with CLL progression even before Ibrutinib treatment.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146356
Arroyo, Daniela Soledad; Rodríguez, Cecilia Inés; Bussi, Claudio; Manzone Rodriguez, Clarisa; Sastre, Darío; et al.; Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia; Frontiers Media; Frontiers in Endocrinology; 11; 321; 7-2020; 1-6
1664-2392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146356
identifier_str_mv Arroyo, Daniela Soledad; Rodríguez, Cecilia Inés; Bussi, Claudio; Manzone Rodriguez, Clarisa; Sastre, Darío; et al.; Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia; Frontiers Media; Frontiers in Endocrinology; 11; 321; 7-2020; 1-6
1664-2392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2020.00321
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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