In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides

Autores
Machuca, Laura Marcela; Acuña Ojeda, Maria Florencia; Alfaro, Esteban; Spagnolo, Lorena Cecilia; Obregon, Veronica Gabriela; Ibañez, Julia Magali; Murguia, Marcelo Cesar
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tomato cultivation is an important agricultural activity in northeast of Argentina, and disease control is indispensable for its production. The purpose of the current study was to identify two fungi isolated from tomato plants cultivated in greenhouses, with symptoms of leaf mould disease and a strain of the genus Cladosporium from a culture collection, and evaluate their in vitro susceptibility to four commercial fungicides. Macro and microscopic examination, molecular characterisation and sequence analysis were applied for identification. Broth dilution and spread plate methods were used to determine the minimal inhibitory concentration (MIC) and minimal fungicide concentration (MFC). The active ingredients of the products were azoxystrobin+difenoconazole, trifloxystrobin+tebuconazole, chlorothalonil and metalaxyl-M+mancozeb. The results were processed using the Kruskal-Wallis method. The isolates were identified as Cladosporium cladosporioidesa-c; consequently, lesions found on tomato plants did not correspond to Cladosporium fulvum. There was a significant statistical difference between the obtained values. Qualitatively, the three strains had a similar behaviour for chlorothalonil (MIC values: 0.25 - 0.5 μg/ml, MFC values: 4 μg/ml). In all cases, tests with metalaxyl-M+mancozeb yielded higher values than those achieved for chlorothalonil (MIC values: 8 μg/ml, MFC values: 8- 32 μg/ml). trobilurin-formulated fungicides were less effective against C. cladosporioidesa-b (MIC values: 16-256 μg/ml, MFC values: >64 μg/ml). C. cladosporioidesc was the most sensitive isolate. The information about the presence of a non-frequent fungus and its fungicide susceptibility, would be useful for establishing control strategies and enhance production.
Fil: Machuca, Laura Marcela. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acuña Ojeda, Maria Florencia. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfaro, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina
Fil: Spagnolo, Lorena Cecilia. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Corrientes. Estacion Experimental Agropecuaria Bella Vista. Agencia de Extension Rural Goya.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obregon, Veronica Gabriela. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina
Fil: Ibañez, Julia Magali. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Corrientes. Estacion Experimental Agropecuaria Bella Vista. Agencia de Extension Rural Goya.; Argentina
Fil: Murguia, Marcelo Cesar. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Química Aplicada.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Antifungals
characterisation
Cladosporium cladosporioides
greenhouse
Solanum lycopersicum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232040
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232040
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicidesMachuca, Laura MarcelaAcuña Ojeda, Maria FlorenciaAlfaro, EstebanSpagnolo, Lorena CeciliaObregon, Veronica GabrielaIbañez, Julia MagaliMurguia, Marcelo CesarAntifungalscharacterisationCladosporium cladosporioidesgreenhouseSolanum lycopersicumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tomato cultivation is an important agricultural activity in northeast of Argentina, and disease control is indispensable for its production. The purpose of the current study was to identify two fungi isolated from tomato plants cultivated in greenhouses, with symptoms of leaf mould disease and a strain of the genus Cladosporium from a culture collection, and evaluate their in vitro susceptibility to four commercial fungicides. Macro and microscopic examination, molecular characterisation and sequence analysis were applied for identification. Broth dilution and spread plate methods were used to determine the minimal inhibitory concentration (MIC) and minimal fungicide concentration (MFC). The active ingredients of the products were azoxystrobin+difenoconazole, trifloxystrobin+tebuconazole, chlorothalonil and metalaxyl-M+mancozeb. The results were processed using the Kruskal-Wallis method. The isolates were identified as Cladosporium cladosporioidesa-c; consequently, lesions found on tomato plants did not correspond to Cladosporium fulvum. There was a significant statistical difference between the obtained values. Qualitatively, the three strains had a similar behaviour for chlorothalonil (MIC values: 0.25 - 0.5 μg/ml, MFC values: 4 μg/ml). In all cases, tests with metalaxyl-M+mancozeb yielded higher values than those achieved for chlorothalonil (MIC values: 8 μg/ml, MFC values: 8- 32 μg/ml). trobilurin-formulated fungicides were less effective against C. cladosporioidesa-b (MIC values: 16-256 μg/ml, MFC values: >64 μg/ml). C. cladosporioidesc was the most sensitive isolate. The information about the presence of a non-frequent fungus and its fungicide susceptibility, would be useful for establishing control strategies and enhance production.Fil: Machuca, Laura Marcela. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Acuña Ojeda, Maria Florencia. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alfaro, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); ArgentinaFil: Spagnolo, Lorena Cecilia. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Corrientes. Estacion Experimental Agropecuaria Bella Vista. Agencia de Extension Rural Goya.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Obregon, Veronica Gabriela. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); ArgentinaFil: Ibañez, Julia Magali. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Corrientes. Estacion Experimental Agropecuaria Bella Vista. Agencia de Extension Rural Goya.; ArgentinaFil: Murguia, Marcelo Cesar. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Química Aplicada.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaSociety for Promotion of Horticulture2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232040Machuca, Laura Marcela; Acuña Ojeda, Maria Florencia; Alfaro, Esteban; Spagnolo, Lorena Cecilia; Obregon, Veronica Gabriela; et al.; In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides; Society for Promotion of Horticulture; Journal of Horticultural Sciences; 18; 2; 11-2023; 432-4380973-354XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://jhs.iihr.res.in/index.php/jhs/article/view/1642info:eu-repo/semantics/altIdentifier/doi/10.24154/jhs.v18i2.1642info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:03Zoai:ri.conicet.gov.ar:11336/232040instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:04.208CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
title In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
spellingShingle In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
Machuca, Laura Marcela
Antifungals
characterisation
Cladosporium cladosporioides
greenhouse
Solanum lycopersicum
title_short In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
title_full In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
title_fullStr In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
title_full_unstemmed In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
title_sort In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides
dc.creator.none.fl_str_mv Machuca, Laura Marcela
Acuña Ojeda, Maria Florencia
Alfaro, Esteban
Spagnolo, Lorena Cecilia
Obregon, Veronica Gabriela
Ibañez, Julia Magali
Murguia, Marcelo Cesar
author Machuca, Laura Marcela
author_facet Machuca, Laura Marcela
Acuña Ojeda, Maria Florencia
Alfaro, Esteban
Spagnolo, Lorena Cecilia
Obregon, Veronica Gabriela
Ibañez, Julia Magali
Murguia, Marcelo Cesar
author_role author
author2 Acuña Ojeda, Maria Florencia
Alfaro, Esteban
Spagnolo, Lorena Cecilia
Obregon, Veronica Gabriela
Ibañez, Julia Magali
Murguia, Marcelo Cesar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antifungals
characterisation
Cladosporium cladosporioides
greenhouse
Solanum lycopersicum
topic Antifungals
characterisation
Cladosporium cladosporioides
greenhouse
Solanum lycopersicum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tomato cultivation is an important agricultural activity in northeast of Argentina, and disease control is indispensable for its production. The purpose of the current study was to identify two fungi isolated from tomato plants cultivated in greenhouses, with symptoms of leaf mould disease and a strain of the genus Cladosporium from a culture collection, and evaluate their in vitro susceptibility to four commercial fungicides. Macro and microscopic examination, molecular characterisation and sequence analysis were applied for identification. Broth dilution and spread plate methods were used to determine the minimal inhibitory concentration (MIC) and minimal fungicide concentration (MFC). The active ingredients of the products were azoxystrobin+difenoconazole, trifloxystrobin+tebuconazole, chlorothalonil and metalaxyl-M+mancozeb. The results were processed using the Kruskal-Wallis method. The isolates were identified as Cladosporium cladosporioidesa-c; consequently, lesions found on tomato plants did not correspond to Cladosporium fulvum. There was a significant statistical difference between the obtained values. Qualitatively, the three strains had a similar behaviour for chlorothalonil (MIC values: 0.25 - 0.5 μg/ml, MFC values: 4 μg/ml). In all cases, tests with metalaxyl-M+mancozeb yielded higher values than those achieved for chlorothalonil (MIC values: 8 μg/ml, MFC values: 8- 32 μg/ml). trobilurin-formulated fungicides were less effective against C. cladosporioidesa-b (MIC values: 16-256 μg/ml, MFC values: >64 μg/ml). C. cladosporioidesc was the most sensitive isolate. The information about the presence of a non-frequent fungus and its fungicide susceptibility, would be useful for establishing control strategies and enhance production.
Fil: Machuca, Laura Marcela. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acuña Ojeda, Maria Florencia. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfaro, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina
Fil: Spagnolo, Lorena Cecilia. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Corrientes. Estacion Experimental Agropecuaria Bella Vista. Agencia de Extension Rural Goya.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obregon, Veronica Gabriela. Universidad de la Cuenca del Plata. Secretaria de Politicas del Conocimiento. Instituto de Investigaciones Científicas (sede Goya); Argentina
Fil: Ibañez, Julia Magali. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Corrientes. Estacion Experimental Agropecuaria Bella Vista. Agencia de Extension Rural Goya.; Argentina
Fil: Murguia, Marcelo Cesar. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Química Aplicada.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description Tomato cultivation is an important agricultural activity in northeast of Argentina, and disease control is indispensable for its production. The purpose of the current study was to identify two fungi isolated from tomato plants cultivated in greenhouses, with symptoms of leaf mould disease and a strain of the genus Cladosporium from a culture collection, and evaluate their in vitro susceptibility to four commercial fungicides. Macro and microscopic examination, molecular characterisation and sequence analysis were applied for identification. Broth dilution and spread plate methods were used to determine the minimal inhibitory concentration (MIC) and minimal fungicide concentration (MFC). The active ingredients of the products were azoxystrobin+difenoconazole, trifloxystrobin+tebuconazole, chlorothalonil and metalaxyl-M+mancozeb. The results were processed using the Kruskal-Wallis method. The isolates were identified as Cladosporium cladosporioidesa-c; consequently, lesions found on tomato plants did not correspond to Cladosporium fulvum. There was a significant statistical difference between the obtained values. Qualitatively, the three strains had a similar behaviour for chlorothalonil (MIC values: 0.25 - 0.5 μg/ml, MFC values: 4 μg/ml). In all cases, tests with metalaxyl-M+mancozeb yielded higher values than those achieved for chlorothalonil (MIC values: 8 μg/ml, MFC values: 8- 32 μg/ml). trobilurin-formulated fungicides were less effective against C. cladosporioidesa-b (MIC values: 16-256 μg/ml, MFC values: >64 μg/ml). C. cladosporioidesc was the most sensitive isolate. The information about the presence of a non-frequent fungus and its fungicide susceptibility, would be useful for establishing control strategies and enhance production.
publishDate 2023
dc.date.none.fl_str_mv 2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232040
Machuca, Laura Marcela; Acuña Ojeda, Maria Florencia; Alfaro, Esteban; Spagnolo, Lorena Cecilia; Obregon, Veronica Gabriela; et al.; In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides; Society for Promotion of Horticulture; Journal of Horticultural Sciences; 18; 2; 11-2023; 432-438
0973-354X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232040
identifier_str_mv Machuca, Laura Marcela; Acuña Ojeda, Maria Florencia; Alfaro, Esteban; Spagnolo, Lorena Cecilia; Obregon, Veronica Gabriela; et al.; In vitro susceptibility test of Cladosporium cladosporioides isolates from Argentinian tomato crops against commercial fungicides; Society for Promotion of Horticulture; Journal of Horticultural Sciences; 18; 2; 11-2023; 432-438
0973-354X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://jhs.iihr.res.in/index.php/jhs/article/view/1642
info:eu-repo/semantics/altIdentifier/doi/10.24154/jhs.v18i2.1642
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Promotion of Horticulture
publisher.none.fl_str_mv Society for Promotion of Horticulture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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