p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy
- Autores
- Barcelona, Pablo Federico; Sitaras, Nicholas; Galan, Alba; Esquiva, Gema; Jmaeff, Sean; Jian, Yiman; Sarunic, Marinko V.; Cuenca, Nicolas; Sapieha, Przemyslaw; Saragovi, H. Uri
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In many diseases, expression and ligand-dependent activity of the p75NTR receptor can promote pericyte and vascular dysfunction, inflammation, glial activation, and neurodegeneration. Diabetic retinopathy (DR) is characterized by all of these pathological events. However, the mechanisms by which p75NTR may be implicated at each stage of DR pathology remain poorly understood. Here, in a streptozotocin mouse model of diabetic retinopathy, we report that p75NTR is up-regulated very early in glia and in pericytes to mediate ligand-dependent induction of inflammatory cytokines, disruption of the neuro-glia-vascular unit, promotion of blood-retina-barrier breakdown, edema, and neuronal death. In a mouse model of oxygen-induced retinopathy, mimicking proliferative DR, p75NTR?dependent inflammation leads to ischemia and pathological angiogenesis through Semaphorin 3A. The acute use of antagonists of p75NTR or antagonists of the ligand proNGF suppresses each distinct phase of pathology, ameliorate disease, and prevent disease progression. Thus, our study documents novel disease mechanisms, and validates druggable targets for diabetic retinopathy.
Fil: Barcelona, Pablo Federico. Mc Gill University. Lady Davis Research Intitute; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sitaras, Nicholas. University of Montreal; Canadá
Fil: Galan, Alba. Mc Gill University. Lady Davis Research Intitute; Canadá
Fil: Esquiva, Gema. Universidad de Alicante; España
Fil: Jmaeff, Sean. Mc Gill University. Lady Davis Research Intitute; Canadá
Fil: Jian, Yiman. University Fraser Simon; Canadá
Fil: Sarunic, Marinko V.. University Fraser Simon; Canadá
Fil: Cuenca, Nicolas. Universidad de Alicante. Facultad de Ciencias; España
Fil: Sapieha, Przemyslaw. University of Montreal; Canadá
Fil: Saragovi, H. Uri. Mc Gill University. Lady Davis Research Intitute; Canadá - Materia
-
P75 NTR
ALFA2M
DIABETES
PRONGF - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/48048
Ver los metadatos del registro completo
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network_name_str |
CONICET Digital (CONICET) |
spelling |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathyBarcelona, Pablo FedericoSitaras, NicholasGalan, AlbaEsquiva, GemaJmaeff, SeanJian, YimanSarunic, Marinko V.Cuenca, NicolasSapieha, PrzemyslawSaragovi, H. UriP75 NTRALFA2MDIABETESPRONGFhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In many diseases, expression and ligand-dependent activity of the p75NTR receptor can promote pericyte and vascular dysfunction, inflammation, glial activation, and neurodegeneration. Diabetic retinopathy (DR) is characterized by all of these pathological events. However, the mechanisms by which p75NTR may be implicated at each stage of DR pathology remain poorly understood. Here, in a streptozotocin mouse model of diabetic retinopathy, we report that p75NTR is up-regulated very early in glia and in pericytes to mediate ligand-dependent induction of inflammatory cytokines, disruption of the neuro-glia-vascular unit, promotion of blood-retina-barrier breakdown, edema, and neuronal death. In a mouse model of oxygen-induced retinopathy, mimicking proliferative DR, p75NTR?dependent inflammation leads to ischemia and pathological angiogenesis through Semaphorin 3A. The acute use of antagonists of p75NTR or antagonists of the ligand proNGF suppresses each distinct phase of pathology, ameliorate disease, and prevent disease progression. Thus, our study documents novel disease mechanisms, and validates druggable targets for diabetic retinopathy.Fil: Barcelona, Pablo Federico. Mc Gill University. Lady Davis Research Intitute; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Sitaras, Nicholas. University of Montreal; CanadáFil: Galan, Alba. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Esquiva, Gema. Universidad de Alicante; EspañaFil: Jmaeff, Sean. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Jian, Yiman. University Fraser Simon; CanadáFil: Sarunic, Marinko V.. University Fraser Simon; CanadáFil: Cuenca, Nicolas. Universidad de Alicante. Facultad de Ciencias; EspañaFil: Sapieha, Przemyslaw. University of Montreal; CanadáFil: Saragovi, H. Uri. Mc Gill University. Lady Davis Research Intitute; CanadáSociety for Neuroscience2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48048Barcelona, Pablo Federico; Sitaras, Nicholas; Galan, Alba; Esquiva, Gema; Jmaeff, Sean; et al.; p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy; Society for Neuroscience; Journal of Neuroscience; 36; 34; 8-2016; 8826-88410270-64741529-2401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/36/34/8826info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4278-15.2016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:04Zoai:ri.conicet.gov.ar:11336/48048instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:04.334CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
title |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
spellingShingle |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy Barcelona, Pablo Federico P75 NTR ALFA2M DIABETES PRONGF |
title_short |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
title_full |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
title_fullStr |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
title_full_unstemmed |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
title_sort |
p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy |
dc.creator.none.fl_str_mv |
Barcelona, Pablo Federico Sitaras, Nicholas Galan, Alba Esquiva, Gema Jmaeff, Sean Jian, Yiman Sarunic, Marinko V. Cuenca, Nicolas Sapieha, Przemyslaw Saragovi, H. Uri |
author |
Barcelona, Pablo Federico |
author_facet |
Barcelona, Pablo Federico Sitaras, Nicholas Galan, Alba Esquiva, Gema Jmaeff, Sean Jian, Yiman Sarunic, Marinko V. Cuenca, Nicolas Sapieha, Przemyslaw Saragovi, H. Uri |
author_role |
author |
author2 |
Sitaras, Nicholas Galan, Alba Esquiva, Gema Jmaeff, Sean Jian, Yiman Sarunic, Marinko V. Cuenca, Nicolas Sapieha, Przemyslaw Saragovi, H. Uri |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
P75 NTR ALFA2M DIABETES PRONGF |
topic |
P75 NTR ALFA2M DIABETES PRONGF |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In many diseases, expression and ligand-dependent activity of the p75NTR receptor can promote pericyte and vascular dysfunction, inflammation, glial activation, and neurodegeneration. Diabetic retinopathy (DR) is characterized by all of these pathological events. However, the mechanisms by which p75NTR may be implicated at each stage of DR pathology remain poorly understood. Here, in a streptozotocin mouse model of diabetic retinopathy, we report that p75NTR is up-regulated very early in glia and in pericytes to mediate ligand-dependent induction of inflammatory cytokines, disruption of the neuro-glia-vascular unit, promotion of blood-retina-barrier breakdown, edema, and neuronal death. In a mouse model of oxygen-induced retinopathy, mimicking proliferative DR, p75NTR?dependent inflammation leads to ischemia and pathological angiogenesis through Semaphorin 3A. The acute use of antagonists of p75NTR or antagonists of the ligand proNGF suppresses each distinct phase of pathology, ameliorate disease, and prevent disease progression. Thus, our study documents novel disease mechanisms, and validates druggable targets for diabetic retinopathy. Fil: Barcelona, Pablo Federico. Mc Gill University. Lady Davis Research Intitute; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Sitaras, Nicholas. University of Montreal; Canadá Fil: Galan, Alba. Mc Gill University. Lady Davis Research Intitute; Canadá Fil: Esquiva, Gema. Universidad de Alicante; España Fil: Jmaeff, Sean. Mc Gill University. Lady Davis Research Intitute; Canadá Fil: Jian, Yiman. University Fraser Simon; Canadá Fil: Sarunic, Marinko V.. University Fraser Simon; Canadá Fil: Cuenca, Nicolas. Universidad de Alicante. Facultad de Ciencias; España Fil: Sapieha, Przemyslaw. University of Montreal; Canadá Fil: Saragovi, H. Uri. Mc Gill University. Lady Davis Research Intitute; Canadá |
description |
In many diseases, expression and ligand-dependent activity of the p75NTR receptor can promote pericyte and vascular dysfunction, inflammation, glial activation, and neurodegeneration. Diabetic retinopathy (DR) is characterized by all of these pathological events. However, the mechanisms by which p75NTR may be implicated at each stage of DR pathology remain poorly understood. Here, in a streptozotocin mouse model of diabetic retinopathy, we report that p75NTR is up-regulated very early in glia and in pericytes to mediate ligand-dependent induction of inflammatory cytokines, disruption of the neuro-glia-vascular unit, promotion of blood-retina-barrier breakdown, edema, and neuronal death. In a mouse model of oxygen-induced retinopathy, mimicking proliferative DR, p75NTR?dependent inflammation leads to ischemia and pathological angiogenesis through Semaphorin 3A. The acute use of antagonists of p75NTR or antagonists of the ligand proNGF suppresses each distinct phase of pathology, ameliorate disease, and prevent disease progression. Thus, our study documents novel disease mechanisms, and validates druggable targets for diabetic retinopathy. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/48048 Barcelona, Pablo Federico; Sitaras, Nicholas; Galan, Alba; Esquiva, Gema; Jmaeff, Sean; et al.; p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy; Society for Neuroscience; Journal of Neuroscience; 36; 34; 8-2016; 8826-8841 0270-6474 1529-2401 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/48048 |
identifier_str_mv |
Barcelona, Pablo Federico; Sitaras, Nicholas; Galan, Alba; Esquiva, Gema; Jmaeff, Sean; et al.; p75NTR and its ligand proNGF activate paracrine mechanisms etiological to the vascular, inflammatory, and neurodegenerative pathologies of diabetic retinopathy; Society for Neuroscience; Journal of Neuroscience; 36; 34; 8-2016; 8826-8841 0270-6474 1529-2401 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/36/34/8826 info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4278-15.2016 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for Neuroscience |
publisher.none.fl_str_mv |
Society for Neuroscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613201991303168 |
score |
13.070432 |