Cross-talk between rapid and long term effects of progesterone on vascular tissue

Autores
Cutini, Pablo Hernan; Selles Tasa, Juana; Massheimer, Virginia Laura
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We tested the hypothesis whether; the non-genomic action of progesterone (Pg) on vascular tissue would be associated with hormonal long term effect on the modulation of cell growth. Using rat aortic strips, we showed that the stimulatory effect of Pg on nitric oxide synthesis involved both kinase and phosphatase pathways. The increase in the vasoactive production was prevented by the MAPK inhibitor (PD98059). In addition, preincubation with a phosphatase antagonist potentiated the hormonal effect. Pg increased PKC activity, but the inhibition of PKC did not alter the stimulatory action of the hormone on nitric oxide generation. In endothelial cell cultures (EC), 24 h treatment with Pg significantly diminished cell proliferation. This antiproliferative effect was suppressed by the PKC inhibitor chelerythrine (chel) and l-NAME (nitric oxide synthase inhibitor). We also observed that Pg stimulates EC migration. In summary, the present findings provide evidence of an integration of genomic and non-genomic effects in the mechanism of action displayed by Pg in vascular tissue. The fast effects elicited by the hormone implies signal transduction activation required for the regulation of vasoactive production, but also necessary for the modulation of endothelial cells growth.
Fil: Cutini, Pablo Hernan. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Selles Tasa, Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina
Materia
Cell Migration
Cell Proliferation
Endothelial Cell
Nitric Oxide
Progesterone
Protein Kinase C
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/73616
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/73616
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cross-talk between rapid and long term effects of progesterone on vascular tissueCutini, Pablo HernanSelles Tasa, JuanaMassheimer, Virginia LauraCell MigrationCell ProliferationEndothelial CellNitric OxideProgesteroneProtein Kinase Chttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We tested the hypothesis whether; the non-genomic action of progesterone (Pg) on vascular tissue would be associated with hormonal long term effect on the modulation of cell growth. Using rat aortic strips, we showed that the stimulatory effect of Pg on nitric oxide synthesis involved both kinase and phosphatase pathways. The increase in the vasoactive production was prevented by the MAPK inhibitor (PD98059). In addition, preincubation with a phosphatase antagonist potentiated the hormonal effect. Pg increased PKC activity, but the inhibition of PKC did not alter the stimulatory action of the hormone on nitric oxide generation. In endothelial cell cultures (EC), 24 h treatment with Pg significantly diminished cell proliferation. This antiproliferative effect was suppressed by the PKC inhibitor chelerythrine (chel) and l-NAME (nitric oxide synthase inhibitor). We also observed that Pg stimulates EC migration. In summary, the present findings provide evidence of an integration of genomic and non-genomic effects in the mechanism of action displayed by Pg in vascular tissue. The fast effects elicited by the hormone implies signal transduction activation required for the regulation of vasoactive production, but also necessary for the modulation of endothelial cells growth.Fil: Cutini, Pablo Hernan. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Selles Tasa, Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; ArgentinaPergamon-Elsevier Science Ltd2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/73616Cutini, Pablo Hernan; Selles Tasa, Juana; Massheimer, Virginia Laura; Cross-talk between rapid and long term effects of progesterone on vascular tissue; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 115; 1-2; 5-2009; 36-430960-0760CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2009.02.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960076009000648info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:14Zoai:ri.conicet.gov.ar:11336/73616instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:14.405CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cross-talk between rapid and long term effects of progesterone on vascular tissue
title Cross-talk between rapid and long term effects of progesterone on vascular tissue
spellingShingle Cross-talk between rapid and long term effects of progesterone on vascular tissue
Cutini, Pablo Hernan
Cell Migration
Cell Proliferation
Endothelial Cell
Nitric Oxide
Progesterone
Protein Kinase C
title_short Cross-talk between rapid and long term effects of progesterone on vascular tissue
title_full Cross-talk between rapid and long term effects of progesterone on vascular tissue
title_fullStr Cross-talk between rapid and long term effects of progesterone on vascular tissue
title_full_unstemmed Cross-talk between rapid and long term effects of progesterone on vascular tissue
title_sort Cross-talk between rapid and long term effects of progesterone on vascular tissue
dc.creator.none.fl_str_mv Cutini, Pablo Hernan
Selles Tasa, Juana
Massheimer, Virginia Laura
author Cutini, Pablo Hernan
author_facet Cutini, Pablo Hernan
Selles Tasa, Juana
Massheimer, Virginia Laura
author_role author
author2 Selles Tasa, Juana
Massheimer, Virginia Laura
author2_role author
author
dc.subject.none.fl_str_mv Cell Migration
Cell Proliferation
Endothelial Cell
Nitric Oxide
Progesterone
Protein Kinase C
topic Cell Migration
Cell Proliferation
Endothelial Cell
Nitric Oxide
Progesterone
Protein Kinase C
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We tested the hypothesis whether; the non-genomic action of progesterone (Pg) on vascular tissue would be associated with hormonal long term effect on the modulation of cell growth. Using rat aortic strips, we showed that the stimulatory effect of Pg on nitric oxide synthesis involved both kinase and phosphatase pathways. The increase in the vasoactive production was prevented by the MAPK inhibitor (PD98059). In addition, preincubation with a phosphatase antagonist potentiated the hormonal effect. Pg increased PKC activity, but the inhibition of PKC did not alter the stimulatory action of the hormone on nitric oxide generation. In endothelial cell cultures (EC), 24 h treatment with Pg significantly diminished cell proliferation. This antiproliferative effect was suppressed by the PKC inhibitor chelerythrine (chel) and l-NAME (nitric oxide synthase inhibitor). We also observed that Pg stimulates EC migration. In summary, the present findings provide evidence of an integration of genomic and non-genomic effects in the mechanism of action displayed by Pg in vascular tissue. The fast effects elicited by the hormone implies signal transduction activation required for the regulation of vasoactive production, but also necessary for the modulation of endothelial cells growth.
Fil: Cutini, Pablo Hernan. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Selles Tasa, Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Bioquímica Clinica Ii; Argentina
description We tested the hypothesis whether; the non-genomic action of progesterone (Pg) on vascular tissue would be associated with hormonal long term effect on the modulation of cell growth. Using rat aortic strips, we showed that the stimulatory effect of Pg on nitric oxide synthesis involved both kinase and phosphatase pathways. The increase in the vasoactive production was prevented by the MAPK inhibitor (PD98059). In addition, preincubation with a phosphatase antagonist potentiated the hormonal effect. Pg increased PKC activity, but the inhibition of PKC did not alter the stimulatory action of the hormone on nitric oxide generation. In endothelial cell cultures (EC), 24 h treatment with Pg significantly diminished cell proliferation. This antiproliferative effect was suppressed by the PKC inhibitor chelerythrine (chel) and l-NAME (nitric oxide synthase inhibitor). We also observed that Pg stimulates EC migration. In summary, the present findings provide evidence of an integration of genomic and non-genomic effects in the mechanism of action displayed by Pg in vascular tissue. The fast effects elicited by the hormone implies signal transduction activation required for the regulation of vasoactive production, but also necessary for the modulation of endothelial cells growth.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/73616
Cutini, Pablo Hernan; Selles Tasa, Juana; Massheimer, Virginia Laura; Cross-talk between rapid and long term effects of progesterone on vascular tissue; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 115; 1-2; 5-2009; 36-43
0960-0760
CONICET Digital
CONICET
url http://hdl.handle.net/11336/73616
identifier_str_mv Cutini, Pablo Hernan; Selles Tasa, Juana; Massheimer, Virginia Laura; Cross-talk between rapid and long term effects of progesterone on vascular tissue; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 115; 1-2; 5-2009; 36-43
0960-0760
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2009.02.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960076009000648
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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