Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery

Autores
Pasteris, Sergio Enrique; Roig Babot, German; Otero, María Claudia; Bühler, Marta Ines; Nader, Maria Elena Fatima
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work addresses the selection of potentially probiotic lactic acid bacteria (LAB) to be used in raniculture. Thus, strains belonging to the genera Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactococcus lactis and Enterococcus faecium isolated from a Rana catesbeiana hatchery were evaluated for their inhibitory properties against RLS‐associated pathogens (Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus epidermidis) and food‐borne bacteria. Cell‐free supernatants of LAB strains inhibited the growth of at least one of the pathogens by organic acids, but L. lactis CRL 1584 also produced a bacteriocin‐like metabolite. The ability of LAB strains to produce H2O2 in MRS+TMB medium was also studied. Seventy‐eight to ninety six per cent of the strains showed some level of H2O2 production. Moreover, different organic solvents were used to determine the hydrophobicity and Lewis acid/base characteristic of LAB strain surfaces. Most of the strains presented hydrophilic properties, but no acidic or basic surface characters. However, some strains isolated from the skin showed a high degree of hydrophobicity and basic components in the cell surface due to their adhesion to chloroform. These properties were not observed in LAB from balanced feed and freshwater. Taking into account general guidelines and the beneficial properties studied, five strains were selected as potential candidates to be included in a probiotic for raniculture.
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Roig Babot, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bühler, Marta Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Aquaculture
Rana Catesbeiana
Red Leg Syndrome
Lactic Acid Bacteria
Probiotics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47748
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47748
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatcheryPasteris, Sergio EnriqueRoig Babot, GermanOtero, María ClaudiaBühler, Marta InesNader, Maria Elena FatimaAquacultureRana CatesbeianaRed Leg SyndromeLactic Acid BacteriaProbioticshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This work addresses the selection of potentially probiotic lactic acid bacteria (LAB) to be used in raniculture. Thus, strains belonging to the genera Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactococcus lactis and Enterococcus faecium isolated from a Rana catesbeiana hatchery were evaluated for their inhibitory properties against RLS‐associated pathogens (Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus epidermidis) and food‐borne bacteria. Cell‐free supernatants of LAB strains inhibited the growth of at least one of the pathogens by organic acids, but L. lactis CRL 1584 also produced a bacteriocin‐like metabolite. The ability of LAB strains to produce H2O2 in MRS+TMB medium was also studied. Seventy‐eight to ninety six per cent of the strains showed some level of H2O2 production. Moreover, different organic solvents were used to determine the hydrophobicity and Lewis acid/base characteristic of LAB strain surfaces. Most of the strains presented hydrophilic properties, but no acidic or basic surface characters. However, some strains isolated from the skin showed a high degree of hydrophobicity and basic components in the cell surface due to their adhesion to chloroform. These properties were not observed in LAB from balanced feed and freshwater. Taking into account general guidelines and the beneficial properties studied, five strains were selected as potential candidates to be included in a probiotic for raniculture.Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Roig Babot, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Bühler, Marta Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaWiley Blackwell Publishing, Inc2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47748Pasteris, Sergio Enrique; Roig Babot, German; Otero, María Claudia; Bühler, Marta Ines; Nader, Maria Elena Fatima; Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery; Wiley Blackwell Publishing, Inc; Aquaculture Research; 40; 14; 12-2009; 1605-16151355-557X1365-2109CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2109.2009.02261.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2109.2009.02261.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:27:33Zoai:ri.conicet.gov.ar:11336/47748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:27:33.803CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
title Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
spellingShingle Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
Pasteris, Sergio Enrique
Aquaculture
Rana Catesbeiana
Red Leg Syndrome
Lactic Acid Bacteria
Probiotics
title_short Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
title_full Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
title_fullStr Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
title_full_unstemmed Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
title_sort Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery
dc.creator.none.fl_str_mv Pasteris, Sergio Enrique
Roig Babot, German
Otero, María Claudia
Bühler, Marta Ines
Nader, Maria Elena Fatima
author Pasteris, Sergio Enrique
author_facet Pasteris, Sergio Enrique
Roig Babot, German
Otero, María Claudia
Bühler, Marta Ines
Nader, Maria Elena Fatima
author_role author
author2 Roig Babot, German
Otero, María Claudia
Bühler, Marta Ines
Nader, Maria Elena Fatima
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aquaculture
Rana Catesbeiana
Red Leg Syndrome
Lactic Acid Bacteria
Probiotics
topic Aquaculture
Rana Catesbeiana
Red Leg Syndrome
Lactic Acid Bacteria
Probiotics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work addresses the selection of potentially probiotic lactic acid bacteria (LAB) to be used in raniculture. Thus, strains belonging to the genera Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactococcus lactis and Enterococcus faecium isolated from a Rana catesbeiana hatchery were evaluated for their inhibitory properties against RLS‐associated pathogens (Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus epidermidis) and food‐borne bacteria. Cell‐free supernatants of LAB strains inhibited the growth of at least one of the pathogens by organic acids, but L. lactis CRL 1584 also produced a bacteriocin‐like metabolite. The ability of LAB strains to produce H2O2 in MRS+TMB medium was also studied. Seventy‐eight to ninety six per cent of the strains showed some level of H2O2 production. Moreover, different organic solvents were used to determine the hydrophobicity and Lewis acid/base characteristic of LAB strain surfaces. Most of the strains presented hydrophilic properties, but no acidic or basic surface characters. However, some strains isolated from the skin showed a high degree of hydrophobicity and basic components in the cell surface due to their adhesion to chloroform. These properties were not observed in LAB from balanced feed and freshwater. Taking into account general guidelines and the beneficial properties studied, five strains were selected as potential candidates to be included in a probiotic for raniculture.
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Roig Babot, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bühler, Marta Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description This work addresses the selection of potentially probiotic lactic acid bacteria (LAB) to be used in raniculture. Thus, strains belonging to the genera Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactococcus lactis and Enterococcus faecium isolated from a Rana catesbeiana hatchery were evaluated for their inhibitory properties against RLS‐associated pathogens (Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus epidermidis) and food‐borne bacteria. Cell‐free supernatants of LAB strains inhibited the growth of at least one of the pathogens by organic acids, but L. lactis CRL 1584 also produced a bacteriocin‐like metabolite. The ability of LAB strains to produce H2O2 in MRS+TMB medium was also studied. Seventy‐eight to ninety six per cent of the strains showed some level of H2O2 production. Moreover, different organic solvents were used to determine the hydrophobicity and Lewis acid/base characteristic of LAB strain surfaces. Most of the strains presented hydrophilic properties, but no acidic or basic surface characters. However, some strains isolated from the skin showed a high degree of hydrophobicity and basic components in the cell surface due to their adhesion to chloroform. These properties were not observed in LAB from balanced feed and freshwater. Taking into account general guidelines and the beneficial properties studied, five strains were selected as potential candidates to be included in a probiotic for raniculture.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47748
Pasteris, Sergio Enrique; Roig Babot, German; Otero, María Claudia; Bühler, Marta Ines; Nader, Maria Elena Fatima; Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery; Wiley Blackwell Publishing, Inc; Aquaculture Research; 40; 14; 12-2009; 1605-1615
1355-557X
1365-2109
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47748
identifier_str_mv Pasteris, Sergio Enrique; Roig Babot, German; Otero, María Claudia; Bühler, Marta Ines; Nader, Maria Elena Fatima; Beneficial properties of lactic acid bacteria isolated from a Rana catesbeiana hatchery; Wiley Blackwell Publishing, Inc; Aquaculture Research; 40; 14; 12-2009; 1605-1615
1355-557X
1365-2109
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2109.2009.02261.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2109.2009.02261.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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