The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells

Autores
Matzkin, Maria Eugenia; Yamashita, Soichi; Ascoli, Mario
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adult mice with a Leydig cell specific deletion of MAPK kinase (MEK) 1 and 2 (Mek1(f)(/)(f);Mek2(-/-);Cre(+)) mice display Leydig cell hypoplasia and hypergonadotropic hypogonadism. We used radioimmunoassays and quantitative PCR to evaluate the function and expression of the Leydig cell genes involved in the conversion of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic genes. We show that Star, Hsd3b6, Cyp17a1 and Hsd17b3 are downregulated in Ledyig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice whereas Srda1 and Dhrs9 are upregulated and Creb1, Nr5a1, Nr4a1 and Nr0b1 are unchanged or upregulated. Functionally, all the downregulated genes but none of the upregulated genes contribute to the decrease in testosterone synthesis in Leydig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice because they produce low testosterone and dihydrotestosterone when stimulated with hCG or when incubated with testosterone precursors such as progesterone or androstenedione.
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Yamashita, Soichi. University of Iowa; Estados Unidos
Fil: Ascoli, Mario. University of Iowa; Estados Unidos
Materia
ANDROGENS
LEYDIG CELLS
LUTEINIZING HORMONE
MAP KINASE (ERK)
MAPK KINASE (MEK)
STEROIDOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2629
Acceder
id CONICETDig_cdae096bbe492abb5e5fbac36d3b73d0
oai_identifier_str oai:ri.conicet.gov.ar:11336/2629
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cellsMatzkin, Maria EugeniaYamashita, SoichiAscoli, MarioANDROGENSLEYDIG CELLSLUTEINIZING HORMONEMAP KINASE (ERK)MAPK KINASE (MEK)STEROIDOGENESIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Adult mice with a Leydig cell specific deletion of MAPK kinase (MEK) 1 and 2 (Mek1(f)(/)(f);Mek2(-/-);Cre(+)) mice display Leydig cell hypoplasia and hypergonadotropic hypogonadism. We used radioimmunoassays and quantitative PCR to evaluate the function and expression of the Leydig cell genes involved in the conversion of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic genes. We show that Star, Hsd3b6, Cyp17a1 and Hsd17b3 are downregulated in Ledyig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice whereas Srda1 and Dhrs9 are upregulated and Creb1, Nr5a1, Nr4a1 and Nr0b1 are unchanged or upregulated. Functionally, all the downregulated genes but none of the upregulated genes contribute to the decrease in testosterone synthesis in Leydig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice because they produce low testosterone and dihydrotestosterone when stimulated with hCG or when incubated with testosterone precursors such as progesterone or androstenedione.Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Yamashita, Soichi. University of Iowa; Estados UnidosFil: Ascoli, Mario. University of Iowa; Estados UnidosElsevier Ireland2013-03-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/2629Matzkin, Maria Eugenia; Yamashita, Soichi; Ascoli, Mario; The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells; Elsevier Ireland; Molecular And Cellular Endocrinology; 370; 1-2; 7-3-2013; 130-1370303-72071872-8057enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2013.02.017info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713000750info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:45Zoai:ri.conicet.gov.ar:11336/2629instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:45.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
title The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
spellingShingle The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
Matzkin, Maria Eugenia
ANDROGENS
LEYDIG CELLS
LUTEINIZING HORMONE
MAP KINASE (ERK)
MAPK KINASE (MEK)
STEROIDOGENESIS
title_short The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
title_full The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
title_fullStr The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
title_full_unstemmed The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
title_sort The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells
dc.creator.none.fl_str_mv Matzkin, Maria Eugenia
Yamashita, Soichi
Ascoli, Mario
author Matzkin, Maria Eugenia
author_facet Matzkin, Maria Eugenia
Yamashita, Soichi
Ascoli, Mario
author_role author
author2 Yamashita, Soichi
Ascoli, Mario
author2_role author
author
dc.subject.none.fl_str_mv ANDROGENS
LEYDIG CELLS
LUTEINIZING HORMONE
MAP KINASE (ERK)
MAPK KINASE (MEK)
STEROIDOGENESIS
topic ANDROGENS
LEYDIG CELLS
LUTEINIZING HORMONE
MAP KINASE (ERK)
MAPK KINASE (MEK)
STEROIDOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adult mice with a Leydig cell specific deletion of MAPK kinase (MEK) 1 and 2 (Mek1(f)(/)(f);Mek2(-/-);Cre(+)) mice display Leydig cell hypoplasia and hypergonadotropic hypogonadism. We used radioimmunoassays and quantitative PCR to evaluate the function and expression of the Leydig cell genes involved in the conversion of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic genes. We show that Star, Hsd3b6, Cyp17a1 and Hsd17b3 are downregulated in Ledyig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice whereas Srda1 and Dhrs9 are upregulated and Creb1, Nr5a1, Nr4a1 and Nr0b1 are unchanged or upregulated. Functionally, all the downregulated genes but none of the upregulated genes contribute to the decrease in testosterone synthesis in Leydig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice because they produce low testosterone and dihydrotestosterone when stimulated with hCG or when incubated with testosterone precursors such as progesterone or androstenedione.
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Yamashita, Soichi. University of Iowa; Estados Unidos
Fil: Ascoli, Mario. University of Iowa; Estados Unidos
description Adult mice with a Leydig cell specific deletion of MAPK kinase (MEK) 1 and 2 (Mek1(f)(/)(f);Mek2(-/-);Cre(+)) mice display Leydig cell hypoplasia and hypergonadotropic hypogonadism. We used radioimmunoassays and quantitative PCR to evaluate the function and expression of the Leydig cell genes involved in the conversion of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic genes. We show that Star, Hsd3b6, Cyp17a1 and Hsd17b3 are downregulated in Ledyig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice whereas Srda1 and Dhrs9 are upregulated and Creb1, Nr5a1, Nr4a1 and Nr0b1 are unchanged or upregulated. Functionally, all the downregulated genes but none of the upregulated genes contribute to the decrease in testosterone synthesis in Leydig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice because they produce low testosterone and dihydrotestosterone when stimulated with hCG or when incubated with testosterone precursors such as progesterone or androstenedione.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2629
Matzkin, Maria Eugenia; Yamashita, Soichi; Ascoli, Mario; The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells; Elsevier Ireland; Molecular And Cellular Endocrinology; 370; 1-2; 7-3-2013; 130-137
0303-7207
1872-8057
url http://hdl.handle.net/11336/2629
identifier_str_mv Matzkin, Maria Eugenia; Yamashita, Soichi; Ascoli, Mario; The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic enzymes in Leydig cells; Elsevier Ireland; Molecular And Cellular Endocrinology; 370; 1-2; 7-3-2013; 130-137
0303-7207
1872-8057
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2013.02.017
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713000750
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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