Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction

Autores
Sanchez, M.; Alvarenga, Adriana Elizabet; Abate, Carlos Mauricio
Año de publicación
2008
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Marine microorganisms present biotechnological and industrial interest becuase of its capability to develop cold-active biocatalizers, among others. Cold-active enzymes offer economic profits due to incremented reaction rates, high stereospecificity, meager undesirable chemical reactions, lack of heating costs and easy inactivation by its thermal lability. Shewanella is a marine bacterial genus cold-active enzyme producer that presents a big biotechnological potential and grows using a variety of substrates. The aim of present work was to isolate and characterize by restriction profiles, the plasmids present in Shewanella C Q strain, S 2 isolated in our laboratory from Beagle Channel samples, and to build a bridge vector capable of replication in E. coli. For that, plasmidic DNA extractions and conventional and pulsed field gel electrophoresis analyzes were performed. Restriction profile and cloning of selected plasmid were carried out in the pBluescript vector. Results and conclusions: two plasmids of ~3,000 and ~6,000 respectively and a megaplasmid were isolated. Restriction profile of P1 plasmid (3,000 pb) was carried out and obtained fragments were cloned into pBluescript vector, subsequently used in E.coli XL1 blue transformation reactions. The shuttle vector obtained is a valuable tool that would allow to study and express genes of interest of Shewanella’ strains into E. Coli.
Fil: Sanchez, M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Alvarenga, Adriana Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
XLIV Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular
Cordoba
Argentina
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Materia
PLASMID
SHEWANELLA
VECTOR SHUTTLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195552
Acceder
id CONICETDig_cd91b32d484ef6ce335361d52f1233cf
oai_identifier_str oai:ri.conicet.gov.ar:11336/195552
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector constructionSanchez, M.Alvarenga, Adriana ElizabetAbate, Carlos MauricioPLASMIDSHEWANELLAVECTOR SHUTTLEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Marine microorganisms present biotechnological and industrial interest becuase of its capability to develop cold-active biocatalizers, among others. Cold-active enzymes offer economic profits due to incremented reaction rates, high stereospecificity, meager undesirable chemical reactions, lack of heating costs and easy inactivation by its thermal lability. Shewanella is a marine bacterial genus cold-active enzyme producer that presents a big biotechnological potential and grows using a variety of substrates. The aim of present work was to isolate and characterize by restriction profiles, the plasmids present in Shewanella C Q strain, S 2 isolated in our laboratory from Beagle Channel samples, and to build a bridge vector capable of replication in E. coli. For that, plasmidic DNA extractions and conventional and pulsed field gel electrophoresis analyzes were performed. Restriction profile and cloning of selected plasmid were carried out in the pBluescript vector. Results and conclusions: two plasmids of ~3,000 and ~6,000 respectively and a megaplasmid were isolated. Restriction profile of P1 plasmid (3,000 pb) was carried out and obtained fragments were cloned into pBluescript vector, subsequently used in E.coli XL1 blue transformation reactions. The shuttle vector obtained is a valuable tool that would allow to study and express genes of interest of Shewanella’ strains into E. Coli.Fil: Sanchez, M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Alvarenga, Adriana Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaXLIV Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia MolecularCordobaArgentinaSociedad Argentina De Investigación Bioquímica Y Biología MolecularTech Science Press2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195552Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction; XLIV Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular; Cordoba; Argentina; 2008; 57-570327-9545CONICET DigitalCONICETengNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:18Zoai:ri.conicet.gov.ar:11336/195552instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:18.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
title Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
spellingShingle Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
Sanchez, M.
PLASMID
SHEWANELLA
VECTOR SHUTTLE
title_short Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
title_full Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
title_fullStr Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
title_full_unstemmed Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
title_sort Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction
dc.creator.none.fl_str_mv Sanchez, M.
Alvarenga, Adriana Elizabet
Abate, Carlos Mauricio
author Sanchez, M.
author_facet Sanchez, M.
Alvarenga, Adriana Elizabet
Abate, Carlos Mauricio
author_role author
author2 Alvarenga, Adriana Elizabet
Abate, Carlos Mauricio
author2_role author
author
dc.subject.none.fl_str_mv PLASMID
SHEWANELLA
VECTOR SHUTTLE
topic PLASMID
SHEWANELLA
VECTOR SHUTTLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Marine microorganisms present biotechnological and industrial interest becuase of its capability to develop cold-active biocatalizers, among others. Cold-active enzymes offer economic profits due to incremented reaction rates, high stereospecificity, meager undesirable chemical reactions, lack of heating costs and easy inactivation by its thermal lability. Shewanella is a marine bacterial genus cold-active enzyme producer that presents a big biotechnological potential and grows using a variety of substrates. The aim of present work was to isolate and characterize by restriction profiles, the plasmids present in Shewanella C Q strain, S 2 isolated in our laboratory from Beagle Channel samples, and to build a bridge vector capable of replication in E. coli. For that, plasmidic DNA extractions and conventional and pulsed field gel electrophoresis analyzes were performed. Restriction profile and cloning of selected plasmid were carried out in the pBluescript vector. Results and conclusions: two plasmids of ~3,000 and ~6,000 respectively and a megaplasmid were isolated. Restriction profile of P1 plasmid (3,000 pb) was carried out and obtained fragments were cloned into pBluescript vector, subsequently used in E.coli XL1 blue transformation reactions. The shuttle vector obtained is a valuable tool that would allow to study and express genes of interest of Shewanella’ strains into E. Coli.
Fil: Sanchez, M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Alvarenga, Adriana Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
XLIV Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular
Cordoba
Argentina
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
description Marine microorganisms present biotechnological and industrial interest becuase of its capability to develop cold-active biocatalizers, among others. Cold-active enzymes offer economic profits due to incremented reaction rates, high stereospecificity, meager undesirable chemical reactions, lack of heating costs and easy inactivation by its thermal lability. Shewanella is a marine bacterial genus cold-active enzyme producer that presents a big biotechnological potential and grows using a variety of substrates. The aim of present work was to isolate and characterize by restriction profiles, the plasmids present in Shewanella C Q strain, S 2 isolated in our laboratory from Beagle Channel samples, and to build a bridge vector capable of replication in E. coli. For that, plasmidic DNA extractions and conventional and pulsed field gel electrophoresis analyzes were performed. Restriction profile and cloning of selected plasmid were carried out in the pBluescript vector. Results and conclusions: two plasmids of ~3,000 and ~6,000 respectively and a megaplasmid were isolated. Restriction profile of P1 plasmid (3,000 pb) was carried out and obtained fragments were cloned into pBluescript vector, subsequently used in E.coli XL1 blue transformation reactions. The shuttle vector obtained is a valuable tool that would allow to study and express genes of interest of Shewanella’ strains into E. Coli.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195552
Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction; XLIV Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular; Cordoba; Argentina; 2008; 57-57
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195552
identifier_str_mv Isolation and characterization of plasmids from Shewanella CSQ2. A shuttle vector construction; XLIV Reunion Anual de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular; Cordoba; Argentina; 2008; 57-57
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268966126354432
score 13.13397

Publicaciones similares