Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae

Autores
Sequeiros, Cynthia; Garcés, Marisa Elisabeth; Vallejo, Marisol; Marguet, Emilio R.; Olivera, Nelda Lila
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacteriocin-producing Lactococcus lactis TW34 was isolated from marine fsh. TW34 bacteriocin inhibited the growth of the fsh pathogen Lactococcus garvieae at 5 AU/ml (minimum inhibitory concentration), whereas the minimum bactericidal concentration was 10 AU/ml. Addition of TW34 bacteriocin to L. garvieae cultures resulted in a decrease of six orders of magnitude of viable cells counts demonstrating a bactericidal mode of action. The direct detection of the bacteriocin activity by Tricine-SDS-PAGE showed an active peptide with a molec- ular mass ca. 4.5 kDa. The analysis by MALDI-TOF-MS detected a strong signal at m/z 2,351.2 that corresponded to the nisin leader peptide mass without the initiating methio- nine, whose sequence STKDFNLDLVSVSKKDSGASPR was confrmed by MS/MS. Sequence analysis of nisin structural gene confrmed that L. lactis TW34 was a nisin Z producer. This nisin Z-producing strain with probiotic properties might be considered as an alternative in the pre- vention of lactococcosis, a global disease in aquaculture systems.
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Vallejo, Marisol. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; Argentina
Fil: Marguet, Emilio R.. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Materia
Probiotic
Lactic Acid Bacteria
Bacteriocin
Lactococcus Lactis
Lactococcus Garvieae
Fish Pathogen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10356
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10356
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieaeSequeiros, CynthiaGarcés, Marisa ElisabethVallejo, MarisolMarguet, Emilio R.Olivera, Nelda LilaProbioticLactic Acid BacteriaBacteriocinLactococcus LactisLactococcus GarvieaeFish Pathogenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacteriocin-producing Lactococcus lactis TW34 was isolated from marine fsh. TW34 bacteriocin inhibited the growth of the fsh pathogen Lactococcus garvieae at 5 AU/ml (minimum inhibitory concentration), whereas the minimum bactericidal concentration was 10 AU/ml. Addition of TW34 bacteriocin to L. garvieae cultures resulted in a decrease of six orders of magnitude of viable cells counts demonstrating a bactericidal mode of action. The direct detection of the bacteriocin activity by Tricine-SDS-PAGE showed an active peptide with a molec- ular mass ca. 4.5 kDa. The analysis by MALDI-TOF-MS detected a strong signal at m/z 2,351.2 that corresponded to the nisin leader peptide mass without the initiating methio- nine, whose sequence STKDFNLDLVSVSKKDSGASPR was confrmed by MS/MS. Sequence analysis of nisin structural gene confrmed that L. lactis TW34 was a nisin Z producer. This nisin Z-producing strain with probiotic properties might be considered as an alternative in the pre- vention of lactococcosis, a global disease in aquaculture systems.Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Vallejo, Marisol. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; ArgentinaFil: Marguet, Emilio R.. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; ArgentinaFil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaSpringer Verlag Berlín2014-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10356Sequeiros, Cynthia; Garcés, Marisa Elisabeth; Vallejo, Marisol; Marguet, Emilio R.; Olivera, Nelda Lila; Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae; Springer Verlag Berlín; Archives Of Microbiology; 197; 3; 31-12-2014; 449–4580302-89331432-072Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-014-1076-xinfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00203-014-1076-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:10Zoai:ri.conicet.gov.ar:11336/10356instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:10.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
title Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
spellingShingle Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
Sequeiros, Cynthia
Probiotic
Lactic Acid Bacteria
Bacteriocin
Lactococcus Lactis
Lactococcus Garvieae
Fish Pathogen
title_short Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
title_full Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
title_fullStr Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
title_full_unstemmed Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
title_sort Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae
dc.creator.none.fl_str_mv Sequeiros, Cynthia
Garcés, Marisa Elisabeth
Vallejo, Marisol
Marguet, Emilio R.
Olivera, Nelda Lila
author Sequeiros, Cynthia
author_facet Sequeiros, Cynthia
Garcés, Marisa Elisabeth
Vallejo, Marisol
Marguet, Emilio R.
Olivera, Nelda Lila
author_role author
author2 Garcés, Marisa Elisabeth
Vallejo, Marisol
Marguet, Emilio R.
Olivera, Nelda Lila
author2_role author
author
author
author
dc.subject.none.fl_str_mv Probiotic
Lactic Acid Bacteria
Bacteriocin
Lactococcus Lactis
Lactococcus Garvieae
Fish Pathogen
topic Probiotic
Lactic Acid Bacteria
Bacteriocin
Lactococcus Lactis
Lactococcus Garvieae
Fish Pathogen
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacteriocin-producing Lactococcus lactis TW34 was isolated from marine fsh. TW34 bacteriocin inhibited the growth of the fsh pathogen Lactococcus garvieae at 5 AU/ml (minimum inhibitory concentration), whereas the minimum bactericidal concentration was 10 AU/ml. Addition of TW34 bacteriocin to L. garvieae cultures resulted in a decrease of six orders of magnitude of viable cells counts demonstrating a bactericidal mode of action. The direct detection of the bacteriocin activity by Tricine-SDS-PAGE showed an active peptide with a molec- ular mass ca. 4.5 kDa. The analysis by MALDI-TOF-MS detected a strong signal at m/z 2,351.2 that corresponded to the nisin leader peptide mass without the initiating methio- nine, whose sequence STKDFNLDLVSVSKKDSGASPR was confrmed by MS/MS. Sequence analysis of nisin structural gene confrmed that L. lactis TW34 was a nisin Z producer. This nisin Z-producing strain with probiotic properties might be considered as an alternative in the pre- vention of lactococcosis, a global disease in aquaculture systems.
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Vallejo, Marisol. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; Argentina
Fil: Marguet, Emilio R.. Universidad Nacional de la Patagonia San Juan Bosco. Sede Trelew; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
description Bacteriocin-producing Lactococcus lactis TW34 was isolated from marine fsh. TW34 bacteriocin inhibited the growth of the fsh pathogen Lactococcus garvieae at 5 AU/ml (minimum inhibitory concentration), whereas the minimum bactericidal concentration was 10 AU/ml. Addition of TW34 bacteriocin to L. garvieae cultures resulted in a decrease of six orders of magnitude of viable cells counts demonstrating a bactericidal mode of action. The direct detection of the bacteriocin activity by Tricine-SDS-PAGE showed an active peptide with a molec- ular mass ca. 4.5 kDa. The analysis by MALDI-TOF-MS detected a strong signal at m/z 2,351.2 that corresponded to the nisin leader peptide mass without the initiating methio- nine, whose sequence STKDFNLDLVSVSKKDSGASPR was confrmed by MS/MS. Sequence analysis of nisin structural gene confrmed that L. lactis TW34 was a nisin Z producer. This nisin Z-producing strain with probiotic properties might be considered as an alternative in the pre- vention of lactococcosis, a global disease in aquaculture systems.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10356
Sequeiros, Cynthia; Garcés, Marisa Elisabeth; Vallejo, Marisol; Marguet, Emilio R.; Olivera, Nelda Lila; Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae; Springer Verlag Berlín; Archives Of Microbiology; 197; 3; 31-12-2014; 449–458
0302-8933
1432-072X
url http://hdl.handle.net/11336/10356
identifier_str_mv Sequeiros, Cynthia; Garcés, Marisa Elisabeth; Vallejo, Marisol; Marguet, Emilio R.; Olivera, Nelda Lila; Potential aquaculture probiont Lactococcus lactis TW34 produces nisin Z and inhibits the fish pathogen Lactococcus garvieae; Springer Verlag Berlín; Archives Of Microbiology; 197; 3; 31-12-2014; 449–458
0302-8933
1432-072X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-014-1076-x
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00203-014-1076-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag Berlín
publisher.none.fl_str_mv Springer Verlag Berlín
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980509357965312
score 13.004268

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