Are there histological changes in the intestine of birds in a stress situation?

Autores
Padrones, Marcelo Nicolás; Filippa, Veronica Palmira; Cid, Fabricio Damian; Chediack, Juan Gabriel
Año de publicación
2020
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Any alteration of homeostatic stability is a stressful situation for living beings. This alteration triggers physiological changes inresponse to a stress situation. In birds this response is related to the release of glucocorticoids. Glucocorticoids influence the functionof several tissues. Although there are a large number of studies that have investigated histological alterations of glucocorticoids inmammals, the knowledge of this area in birds is limited. The main glucocorticoid in birds is corticosterone (CORT). In previousexperiments our research group found a marked relationship between treatment with CORT and an increase in the heterophil tolymphocyte index (H/L), an indicator of stress in birds. We found a decrease in body mass when the birds were treated with CORT;however, the intestine mass was not affected by CORT treatments. The objective of this work was to determine histological alterationin the intestine of Passer domesticus in response to different doses of exogenous CORT. To achieve our goal, 16 sparrows wereacclimated to a laboratory condition with water and food ad libitum, them separating randomly in four groups and each group wereexposed to a different concentration of CORT in drinking water ad libitum (control, 20, 40 and 80 mg/l) for 72 hours emulating a longterm stress situation. After the exposition the intestine was removed and a portion of proximal section was fixed in Bouin solution.Then the intestinal pieces were dehydrated and embedded in paraffin. Histological studies were performed on 5µm sections, stainedby hematoxylin-eosin coloration, and examined by a light microscope. A morphometric analysis was performed using the softwareImage Pro Plus 5.0. The measured parameters were: intestinal perimeter, muscle layer, mucosa thickness, crypt width, villus width andvillus height. The data were statistically analyzed by ANOVA with Tukey post-hoc test (p<0.05). Our results showed a proportionalincrease in the H/L index in concordance with CORT treatments. The crypt width significantly decreased in birds exposed to 80 mg/lof CORT. However, the other parameters did not show significant variation. In conclusion the effect of CORT in the H/L index checksthe effectiveness of the treatment. The decrease in the width of the crypts could be related to an effect at the level of enterocyte renewal.This fact may indicate the first event of a histological alteration in the intestine of Passer domesticus. Supported by CyT-UNSLPROICO 2-0516 and FONCYT PICT-201?0595.
Fil: Padrones, Marcelo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Filippa, Veronica Palmira. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
Intestine
Sress
Birds
Histology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199637
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199637
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Are there histological changes in the intestine of birds in a stress situation?Padrones, Marcelo NicolásFilippa, Veronica PalmiraCid, Fabricio DamianChediack, Juan GabrielIntestineSressBirdsHistologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Any alteration of homeostatic stability is a stressful situation for living beings. This alteration triggers physiological changes inresponse to a stress situation. In birds this response is related to the release of glucocorticoids. Glucocorticoids influence the functionof several tissues. Although there are a large number of studies that have investigated histological alterations of glucocorticoids inmammals, the knowledge of this area in birds is limited. The main glucocorticoid in birds is corticosterone (CORT). In previousexperiments our research group found a marked relationship between treatment with CORT and an increase in the heterophil tolymphocyte index (H/L), an indicator of stress in birds. We found a decrease in body mass when the birds were treated with CORT;however, the intestine mass was not affected by CORT treatments. The objective of this work was to determine histological alterationin the intestine of Passer domesticus in response to different doses of exogenous CORT. To achieve our goal, 16 sparrows wereacclimated to a laboratory condition with water and food ad libitum, them separating randomly in four groups and each group wereexposed to a different concentration of CORT in drinking water ad libitum (control, 20, 40 and 80 mg/l) for 72 hours emulating a longterm stress situation. After the exposition the intestine was removed and a portion of proximal section was fixed in Bouin solution.Then the intestinal pieces were dehydrated and embedded in paraffin. Histological studies were performed on 5µm sections, stainedby hematoxylin-eosin coloration, and examined by a light microscope. A morphometric analysis was performed using the softwareImage Pro Plus 5.0. The measured parameters were: intestinal perimeter, muscle layer, mucosa thickness, crypt width, villus width andvillus height. The data were statistically analyzed by ANOVA with Tukey post-hoc test (p<0.05). Our results showed a proportionalincrease in the H/L index in concordance with CORT treatments. The crypt width significantly decreased in birds exposed to 80 mg/lof CORT. However, the other parameters did not show significant variation. In conclusion the effect of CORT in the H/L index checksthe effectiveness of the treatment. The decrease in the width of the crypts could be related to an effect at the level of enterocyte renewal.This fact may indicate the first event of a histological alteration in the intestine of Passer domesticus. Supported by CyT-UNSLPROICO 2-0516 and FONCYT PICT-201?0595.Fil: Padrones, Marcelo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Filippa, Veronica Palmira. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199637Are there histological changes in the intestine of birds in a stress situation?; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 61-62CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:39:46Zoai:ri.conicet.gov.ar:11336/199637instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:39:47.262CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Are there histological changes in the intestine of birds in a stress situation?
title Are there histological changes in the intestine of birds in a stress situation?
spellingShingle Are there histological changes in the intestine of birds in a stress situation?
Padrones, Marcelo Nicolás
Intestine
Sress
Birds
Histology
title_short Are there histological changes in the intestine of birds in a stress situation?
title_full Are there histological changes in the intestine of birds in a stress situation?
title_fullStr Are there histological changes in the intestine of birds in a stress situation?
title_full_unstemmed Are there histological changes in the intestine of birds in a stress situation?
title_sort Are there histological changes in the intestine of birds in a stress situation?
dc.creator.none.fl_str_mv Padrones, Marcelo Nicolás
Filippa, Veronica Palmira
Cid, Fabricio Damian
Chediack, Juan Gabriel
author Padrones, Marcelo Nicolás
author_facet Padrones, Marcelo Nicolás
Filippa, Veronica Palmira
Cid, Fabricio Damian
Chediack, Juan Gabriel
author_role author
author2 Filippa, Veronica Palmira
Cid, Fabricio Damian
Chediack, Juan Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv Intestine
Sress
Birds
Histology
topic Intestine
Sress
Birds
Histology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Any alteration of homeostatic stability is a stressful situation for living beings. This alteration triggers physiological changes inresponse to a stress situation. In birds this response is related to the release of glucocorticoids. Glucocorticoids influence the functionof several tissues. Although there are a large number of studies that have investigated histological alterations of glucocorticoids inmammals, the knowledge of this area in birds is limited. The main glucocorticoid in birds is corticosterone (CORT). In previousexperiments our research group found a marked relationship between treatment with CORT and an increase in the heterophil tolymphocyte index (H/L), an indicator of stress in birds. We found a decrease in body mass when the birds were treated with CORT;however, the intestine mass was not affected by CORT treatments. The objective of this work was to determine histological alterationin the intestine of Passer domesticus in response to different doses of exogenous CORT. To achieve our goal, 16 sparrows wereacclimated to a laboratory condition with water and food ad libitum, them separating randomly in four groups and each group wereexposed to a different concentration of CORT in drinking water ad libitum (control, 20, 40 and 80 mg/l) for 72 hours emulating a longterm stress situation. After the exposition the intestine was removed and a portion of proximal section was fixed in Bouin solution.Then the intestinal pieces were dehydrated and embedded in paraffin. Histological studies were performed on 5µm sections, stainedby hematoxylin-eosin coloration, and examined by a light microscope. A morphometric analysis was performed using the softwareImage Pro Plus 5.0. The measured parameters were: intestinal perimeter, muscle layer, mucosa thickness, crypt width, villus width andvillus height. The data were statistically analyzed by ANOVA with Tukey post-hoc test (p<0.05). Our results showed a proportionalincrease in the H/L index in concordance with CORT treatments. The crypt width significantly decreased in birds exposed to 80 mg/lof CORT. However, the other parameters did not show significant variation. In conclusion the effect of CORT in the H/L index checksthe effectiveness of the treatment. The decrease in the width of the crypts could be related to an effect at the level of enterocyte renewal.This fact may indicate the first event of a histological alteration in the intestine of Passer domesticus. Supported by CyT-UNSLPROICO 2-0516 and FONCYT PICT-201?0595.
Fil: Padrones, Marcelo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Filippa, Veronica Palmira. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cid, Fabricio Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Chediack, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Any alteration of homeostatic stability is a stressful situation for living beings. This alteration triggers physiological changes inresponse to a stress situation. In birds this response is related to the release of glucocorticoids. Glucocorticoids influence the functionof several tissues. Although there are a large number of studies that have investigated histological alterations of glucocorticoids inmammals, the knowledge of this area in birds is limited. The main glucocorticoid in birds is corticosterone (CORT). In previousexperiments our research group found a marked relationship between treatment with CORT and an increase in the heterophil tolymphocyte index (H/L), an indicator of stress in birds. We found a decrease in body mass when the birds were treated with CORT;however, the intestine mass was not affected by CORT treatments. The objective of this work was to determine histological alterationin the intestine of Passer domesticus in response to different doses of exogenous CORT. To achieve our goal, 16 sparrows wereacclimated to a laboratory condition with water and food ad libitum, them separating randomly in four groups and each group wereexposed to a different concentration of CORT in drinking water ad libitum (control, 20, 40 and 80 mg/l) for 72 hours emulating a longterm stress situation. After the exposition the intestine was removed and a portion of proximal section was fixed in Bouin solution.Then the intestinal pieces were dehydrated and embedded in paraffin. Histological studies were performed on 5µm sections, stainedby hematoxylin-eosin coloration, and examined by a light microscope. A morphometric analysis was performed using the softwareImage Pro Plus 5.0. The measured parameters were: intestinal perimeter, muscle layer, mucosa thickness, crypt width, villus width andvillus height. The data were statistically analyzed by ANOVA with Tukey post-hoc test (p<0.05). Our results showed a proportionalincrease in the H/L index in concordance with CORT treatments. The crypt width significantly decreased in birds exposed to 80 mg/lof CORT. However, the other parameters did not show significant variation. In conclusion the effect of CORT in the H/L index checksthe effectiveness of the treatment. The decrease in the width of the crypts could be related to an effect at the level of enterocyte renewal.This fact may indicate the first event of a histological alteration in the intestine of Passer domesticus. Supported by CyT-UNSLPROICO 2-0516 and FONCYT PICT-201?0595.
publishDate 2020
dc.date.none.fl_str_mv 2020
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info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199637
Are there histological changes in the intestine of birds in a stress situation?; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 61-62
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199637
identifier_str_mv Are there histological changes in the intestine of birds in a stress situation?; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 61-62
CONICET Digital
CONICET
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dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
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