Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease

Autores
Gatto, Emilia Mabel; Riobó, Natalia A.; Carreras, Maria Cecilia; Cherñavsky, Alejandra Claudia; Rubio, Andrea; Satz, M. Leonardo; Poderoso, Juan José
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Much evidence supports a role of nitric oxide (·NO) and peroxynitrite (ONOO-) in experimental and idiopathic Parkinson's disease (PD); moreover, an overexpression of neuronal nitric oxide synthase (nNOS) was recently reported in the basal ganglia of PD patients. In accord, we previously found a 50% increased ·NO production rate during the respiratory burst of circulating neutrophils (PMN) from PD patients. As PMN express the nNOS isoform, the objective of the present study was to ascertain whether this increased ·NO production is representative of nNOS gene upregulation. PMN were isolated from blood samples obtained from seven PD patients and seven age- and sex-matched healthy donors; nNOS mRNA was amplified by reverse transcriptase-polymerase chain reaction and the products were hybridized with a probe for nNOS. Nitrotyrosine-containing proteins and nNOS were detected by Western blot and NO production rate was measured spectrophotometrically by the conversion of oxymyoglobin to metmyoglobin. The results showed that both ·NO production and protein tyrosine nitration were significantly increased in PMN isolated from PD patients (PD 0.09 ± 0.01 vs 0.06 ± 0.008 nmol min-1 106 cells-1; P < 0.05). In addition, five of the seven PD patients showed about 10-fold nNOS mRNA overexpression; while two of the seven PD patients showed an expression level similar to that of the controls; detection of nNOS protein was more evident in the former group. In summary, it is likely that overexpression of nNOS and formation of ONOO- in PMN cells from PD patients emphasizes a potential causal role of ·NO in the physiopathology of the illness. (C) 2000 Academic Press.
Fil: Gatto, Emilia Mabel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina
Fil: Riobó, Natalia A.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina
Fil: Carreras, Maria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cherñavsky, Alejandra Claudia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rubio, Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina
Fil: Satz, M. Leonardo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina
Fil: Poderoso, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina
Materia
Neurodegeneration
Neuronal Nos
Neutrophils
Nitric Oxide
Parkinson'S Disease
Peroxynitrite
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39155
Acceder
id CONICETDig_ccbf69ebbc8e4bfcf136073246147b70
oai_identifier_str oai:ri.conicet.gov.ar:11336/39155
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's diseaseGatto, Emilia MabelRiobó, Natalia A.Carreras, Maria CeciliaCherñavsky, Alejandra ClaudiaRubio, AndreaSatz, M. LeonardoPoderoso, Juan JoséNeurodegenerationNeuronal NosNeutrophilsNitric OxideParkinson'S DiseasePeroxynitritehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Much evidence supports a role of nitric oxide (·NO) and peroxynitrite (ONOO-) in experimental and idiopathic Parkinson's disease (PD); moreover, an overexpression of neuronal nitric oxide synthase (nNOS) was recently reported in the basal ganglia of PD patients. In accord, we previously found a 50% increased ·NO production rate during the respiratory burst of circulating neutrophils (PMN) from PD patients. As PMN express the nNOS isoform, the objective of the present study was to ascertain whether this increased ·NO production is representative of nNOS gene upregulation. PMN were isolated from blood samples obtained from seven PD patients and seven age- and sex-matched healthy donors; nNOS mRNA was amplified by reverse transcriptase-polymerase chain reaction and the products were hybridized with a probe for nNOS. Nitrotyrosine-containing proteins and nNOS were detected by Western blot and NO production rate was measured spectrophotometrically by the conversion of oxymyoglobin to metmyoglobin. The results showed that both ·NO production and protein tyrosine nitration were significantly increased in PMN isolated from PD patients (PD 0.09 ± 0.01 vs 0.06 ± 0.008 nmol min-1 106 cells-1; P < 0.05). In addition, five of the seven PD patients showed about 10-fold nNOS mRNA overexpression; while two of the seven PD patients showed an expression level similar to that of the controls; detection of nNOS protein was more evident in the former group. In summary, it is likely that overexpression of nNOS and formation of ONOO- in PMN cells from PD patients emphasizes a potential causal role of ·NO in the physiopathology of the illness. (C) 2000 Academic Press.Fil: Gatto, Emilia Mabel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; ArgentinaFil: Riobó, Natalia A.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; ArgentinaFil: Carreras, Maria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cherñavsky, Alejandra Claudia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Rubio, Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; ArgentinaFil: Satz, M. Leonardo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; ArgentinaFil: Poderoso, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; ArgentinaAcademic Press Inc Elsevier Science2000-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/39155Gatto, Emilia Mabel; Riobó, Natalia A.; Carreras, Maria Cecilia; Cherñavsky, Alejandra Claudia; Rubio, Andrea; et al.; Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease; Academic Press Inc Elsevier Science; Nitric Oxide-Biology and Chemistry; 4; 5; 6-2000; 534-5391089-8603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1006/niox.2000.0288info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S108986030090288Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-04T11:12:58Zoai:ri.conicet.gov.ar:11336/39155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-04 11:12:58.641CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
title Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
spellingShingle Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
Gatto, Emilia Mabel
Neurodegeneration
Neuronal Nos
Neutrophils
Nitric Oxide
Parkinson'S Disease
Peroxynitrite
title_short Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
title_full Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
title_fullStr Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
title_full_unstemmed Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
title_sort Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease
dc.creator.none.fl_str_mv Gatto, Emilia Mabel
Riobó, Natalia A.
Carreras, Maria Cecilia
Cherñavsky, Alejandra Claudia
Rubio, Andrea
Satz, M. Leonardo
Poderoso, Juan José
author Gatto, Emilia Mabel
author_facet Gatto, Emilia Mabel
Riobó, Natalia A.
Carreras, Maria Cecilia
Cherñavsky, Alejandra Claudia
Rubio, Andrea
Satz, M. Leonardo
Poderoso, Juan José
author_role author
author2 Riobó, Natalia A.
Carreras, Maria Cecilia
Cherñavsky, Alejandra Claudia
Rubio, Andrea
Satz, M. Leonardo
Poderoso, Juan José
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurodegeneration
Neuronal Nos
Neutrophils
Nitric Oxide
Parkinson'S Disease
Peroxynitrite
topic Neurodegeneration
Neuronal Nos
Neutrophils
Nitric Oxide
Parkinson'S Disease
Peroxynitrite
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Much evidence supports a role of nitric oxide (·NO) and peroxynitrite (ONOO-) in experimental and idiopathic Parkinson's disease (PD); moreover, an overexpression of neuronal nitric oxide synthase (nNOS) was recently reported in the basal ganglia of PD patients. In accord, we previously found a 50% increased ·NO production rate during the respiratory burst of circulating neutrophils (PMN) from PD patients. As PMN express the nNOS isoform, the objective of the present study was to ascertain whether this increased ·NO production is representative of nNOS gene upregulation. PMN were isolated from blood samples obtained from seven PD patients and seven age- and sex-matched healthy donors; nNOS mRNA was amplified by reverse transcriptase-polymerase chain reaction and the products were hybridized with a probe for nNOS. Nitrotyrosine-containing proteins and nNOS were detected by Western blot and NO production rate was measured spectrophotometrically by the conversion of oxymyoglobin to metmyoglobin. The results showed that both ·NO production and protein tyrosine nitration were significantly increased in PMN isolated from PD patients (PD 0.09 ± 0.01 vs 0.06 ± 0.008 nmol min-1 106 cells-1; P < 0.05). In addition, five of the seven PD patients showed about 10-fold nNOS mRNA overexpression; while two of the seven PD patients showed an expression level similar to that of the controls; detection of nNOS protein was more evident in the former group. In summary, it is likely that overexpression of nNOS and formation of ONOO- in PMN cells from PD patients emphasizes a potential causal role of ·NO in the physiopathology of the illness. (C) 2000 Academic Press.
Fil: Gatto, Emilia Mabel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina
Fil: Riobó, Natalia A.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina
Fil: Carreras, Maria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cherñavsky, Alejandra Claudia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rubio, Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina
Fil: Satz, M. Leonardo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Inmunogenética; Argentina
Fil: Poderoso, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín. Laboratorio de Metabolismo del Oxígeno; Argentina
description Much evidence supports a role of nitric oxide (·NO) and peroxynitrite (ONOO-) in experimental and idiopathic Parkinson's disease (PD); moreover, an overexpression of neuronal nitric oxide synthase (nNOS) was recently reported in the basal ganglia of PD patients. In accord, we previously found a 50% increased ·NO production rate during the respiratory burst of circulating neutrophils (PMN) from PD patients. As PMN express the nNOS isoform, the objective of the present study was to ascertain whether this increased ·NO production is representative of nNOS gene upregulation. PMN were isolated from blood samples obtained from seven PD patients and seven age- and sex-matched healthy donors; nNOS mRNA was amplified by reverse transcriptase-polymerase chain reaction and the products were hybridized with a probe for nNOS. Nitrotyrosine-containing proteins and nNOS were detected by Western blot and NO production rate was measured spectrophotometrically by the conversion of oxymyoglobin to metmyoglobin. The results showed that both ·NO production and protein tyrosine nitration were significantly increased in PMN isolated from PD patients (PD 0.09 ± 0.01 vs 0.06 ± 0.008 nmol min-1 106 cells-1; P < 0.05). In addition, five of the seven PD patients showed about 10-fold nNOS mRNA overexpression; while two of the seven PD patients showed an expression level similar to that of the controls; detection of nNOS protein was more evident in the former group. In summary, it is likely that overexpression of nNOS and formation of ONOO- in PMN cells from PD patients emphasizes a potential causal role of ·NO in the physiopathology of the illness. (C) 2000 Academic Press.
publishDate 2000
dc.date.none.fl_str_mv 2000-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39155
Gatto, Emilia Mabel; Riobó, Natalia A.; Carreras, Maria Cecilia; Cherñavsky, Alejandra Claudia; Rubio, Andrea; et al.; Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease; Academic Press Inc Elsevier Science; Nitric Oxide-Biology and Chemistry; 4; 5; 6-2000; 534-539
1089-8603
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39155
identifier_str_mv Gatto, Emilia Mabel; Riobó, Natalia A.; Carreras, Maria Cecilia; Cherñavsky, Alejandra Claudia; Rubio, Andrea; et al.; Overexpression of neutrophil neuronal nitric oxide synthase in Parkinson's disease; Academic Press Inc Elsevier Science; Nitric Oxide-Biology and Chemistry; 4; 5; 6-2000; 534-539
1089-8603
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1006/niox.2000.0288
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S108986030090288X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.832306

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