Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970

Autores
Herbert, Nicole A. M.; Kristmundsson, Árni; Vázquez, Nuria Natalia; Hoag, Kelsey; Freeman, Mark A.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Apicomplexa are obligate unicellular parasites found in terrestrial and aquatic environments. Nematopsis are found infect-ing marine invertebrates in a life cycle involving mollusks and crustaceans. In the present study, mantle and muscle tissues fromnerite gastropods were microscopically examined for Nematopsis using wet mounts and histology. Oocysts contained one singlesporozoite surrounded by an ellipsoidal wall and were surrounded by a parasitophorous vacuole within a host phagocyte. Lengthand width of fresh oocysts were measured and compared between host species. DNA was extracted from infected tissue, andregions of the rRNA gene were amplified using novel primers. Nematopsis-infected tissues from scallops from Argentina andScotland were used as controls. The sizes of oocysts observed in nerite hosts from Saint Kitts were not significantly different.DNA sequences of Nematopsis isolated from nerite hosts in this study were identical and phylogenetically related to sequencesobtained from scallops. Bayesian and maximum likelihood phylogenetic analyses robustly place the Nematopsis DNA sequencesfrom this study with Nematopsis from scallops in Florida and members of the family Porosporidae. We conclude that marineNematopsis will group within this clade or within the Porosporidae. We have provided specific oligonucleotide primers to assistwith the molecular study of the Porosporidae.
Fil: Herbert, Nicole A. M.. Ross University; Estados Unidos
Fil: Kristmundsson, Árni. University Of Iceland; Islandia
Fil: Vázquez, Nuria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Hoag, Kelsey. Verandah Pet Hospital,; Estados Unidos
Fil: Freeman, Mark A.. Ross University; Estados Unidos
Materia
Cephaloidophoroidea
gastropod
gregarine
Nematopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277086
Acceder
id CONICETDig_cca9d31453eb1f959ed5c9cffea11500
oai_identifier_str oai:ri.conicet.gov.ar:11336/277086
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970Herbert, Nicole A. M.Kristmundsson, ÁrniVázquez, Nuria NataliaHoag, KelseyFreeman, Mark A.CephaloidophoroideagastropodgregarineNematopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Apicomplexa are obligate unicellular parasites found in terrestrial and aquatic environments. Nematopsis are found infect-ing marine invertebrates in a life cycle involving mollusks and crustaceans. In the present study, mantle and muscle tissues fromnerite gastropods were microscopically examined for Nematopsis using wet mounts and histology. Oocysts contained one singlesporozoite surrounded by an ellipsoidal wall and were surrounded by a parasitophorous vacuole within a host phagocyte. Lengthand width of fresh oocysts were measured and compared between host species. DNA was extracted from infected tissue, andregions of the rRNA gene were amplified using novel primers. Nematopsis-infected tissues from scallops from Argentina andScotland were used as controls. The sizes of oocysts observed in nerite hosts from Saint Kitts were not significantly different.DNA sequences of Nematopsis isolated from nerite hosts in this study were identical and phylogenetically related to sequencesobtained from scallops. Bayesian and maximum likelihood phylogenetic analyses robustly place the Nematopsis DNA sequencesfrom this study with Nematopsis from scallops in Florida and members of the family Porosporidae. We conclude that marineNematopsis will group within this clade or within the Porosporidae. We have provided specific oligonucleotide primers to assistwith the molecular study of the Porosporidae.Fil: Herbert, Nicole A. M.. Ross University; Estados UnidosFil: Kristmundsson, Árni. University Of Iceland; IslandiaFil: Vázquez, Nuria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: Hoag, Kelsey. Verandah Pet Hospital,; Estados UnidosFil: Freeman, Mark A.. Ross University; Estados UnidosWiley Blackwell Publishing, Inc2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277086Herbert, Nicole A. M.; Kristmundsson, Árni; Vázquez, Nuria Natalia; Hoag, Kelsey; Freeman, Mark A.; Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 72; 4; 6-2025; 1-81066-5234CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jeu.70023info:eu-repo/semantics/altIdentifier/doi/10.1111/jeu.70023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-15T10:44:18Zoai:ri.conicet.gov.ar:11336/277086instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-15 10:44:18.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
title Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
spellingShingle Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
Herbert, Nicole A. M.
Cephaloidophoroidea
gastropod
gregarine
Nematopsis
title_short Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
title_full Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
title_fullStr Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
title_full_unstemmed Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
title_sort Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970
dc.creator.none.fl_str_mv Herbert, Nicole A. M.
Kristmundsson, Árni
Vázquez, Nuria Natalia
Hoag, Kelsey
Freeman, Mark A.
author Herbert, Nicole A. M.
author_facet Herbert, Nicole A. M.
Kristmundsson, Árni
Vázquez, Nuria Natalia
Hoag, Kelsey
Freeman, Mark A.
author_role author
author2 Kristmundsson, Árni
Vázquez, Nuria Natalia
Hoag, Kelsey
Freeman, Mark A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cephaloidophoroidea
gastropod
gregarine
Nematopsis
topic Cephaloidophoroidea
gastropod
gregarine
Nematopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Apicomplexa are obligate unicellular parasites found in terrestrial and aquatic environments. Nematopsis are found infect-ing marine invertebrates in a life cycle involving mollusks and crustaceans. In the present study, mantle and muscle tissues fromnerite gastropods were microscopically examined for Nematopsis using wet mounts and histology. Oocysts contained one singlesporozoite surrounded by an ellipsoidal wall and were surrounded by a parasitophorous vacuole within a host phagocyte. Lengthand width of fresh oocysts were measured and compared between host species. DNA was extracted from infected tissue, andregions of the rRNA gene were amplified using novel primers. Nematopsis-infected tissues from scallops from Argentina andScotland were used as controls. The sizes of oocysts observed in nerite hosts from Saint Kitts were not significantly different.DNA sequences of Nematopsis isolated from nerite hosts in this study were identical and phylogenetically related to sequencesobtained from scallops. Bayesian and maximum likelihood phylogenetic analyses robustly place the Nematopsis DNA sequencesfrom this study with Nematopsis from scallops in Florida and members of the family Porosporidae. We conclude that marineNematopsis will group within this clade or within the Porosporidae. We have provided specific oligonucleotide primers to assistwith the molecular study of the Porosporidae.
Fil: Herbert, Nicole A. M.. Ross University; Estados Unidos
Fil: Kristmundsson, Árni. University Of Iceland; Islandia
Fil: Vázquez, Nuria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Hoag, Kelsey. Verandah Pet Hospital,; Estados Unidos
Fil: Freeman, Mark A.. Ross University; Estados Unidos
description The Apicomplexa are obligate unicellular parasites found in terrestrial and aquatic environments. Nematopsis are found infect-ing marine invertebrates in a life cycle involving mollusks and crustaceans. In the present study, mantle and muscle tissues fromnerite gastropods were microscopically examined for Nematopsis using wet mounts and histology. Oocysts contained one singlesporozoite surrounded by an ellipsoidal wall and were surrounded by a parasitophorous vacuole within a host phagocyte. Lengthand width of fresh oocysts were measured and compared between host species. DNA was extracted from infected tissue, andregions of the rRNA gene were amplified using novel primers. Nematopsis-infected tissues from scallops from Argentina andScotland were used as controls. The sizes of oocysts observed in nerite hosts from Saint Kitts were not significantly different.DNA sequences of Nematopsis isolated from nerite hosts in this study were identical and phylogenetically related to sequencesobtained from scallops. Bayesian and maximum likelihood phylogenetic analyses robustly place the Nematopsis DNA sequencesfrom this study with Nematopsis from scallops in Florida and members of the family Porosporidae. We conclude that marineNematopsis will group within this clade or within the Porosporidae. We have provided specific oligonucleotide primers to assistwith the molecular study of the Porosporidae.
publishDate 2025
dc.date.none.fl_str_mv 2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277086
Herbert, Nicole A. M.; Kristmundsson, Árni; Vázquez, Nuria Natalia; Hoag, Kelsey; Freeman, Mark A.; Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 72; 4; 6-2025; 1-8
1066-5234
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277086
identifier_str_mv Herbert, Nicole A. M.; Kristmundsson, Árni; Vázquez, Nuria Natalia; Hoag, Kelsey; Freeman, Mark A.; Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 72; 4; 6-2025; 1-8
1066-5234
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jeu.70023
info:eu-repo/semantics/altIdentifier/doi/10.1111/jeu.70023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1862633508542873600
score 13.203462

Publicaciones similares