Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5
- Autores
- van Drogen, Frank; Mishra, Ranjan; Rudolf, Fabian; Walczak, Michal J.; Lee, Sung Sik; Reiter, Wolfgang; Hegemann, Björn; Pelet, Serge; Dohnal, Ilse; Binolfi, Andrés; Yudina, Zinaida; Selenko, Philipp; Wider, Gerhard; Ammerer, Gustav; Peter, Matthias
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mechanisms remain poorly understood. Here, we show that mechanical stress activates Pkc1, which prevents lysis of pheromone-treated cells by inhibiting polarized growth. In vitro Pkc1 phosphorylates conserved residues within the RING-H2 domains of the scaffold proteins Far1 and Ste5, which are also phosphorylated in vivo. Interestingly, Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3. Indeed, RING phosphorylation interferes with Ste5 membrane association by preventing binding to the receptor-linked Gβγ protein. Cells expressing nonphosphorylatable Ste5 undergo increased lysis upon mechanical stress and exhibit defects in cell-cell fusion during mating, which is exacerbated by simultaneous expression of nonphosphorylatable Far1. These results uncover a mechanical stress-triggered crosstalk mechanism modulating pheromone signaling, polarized growth, and cell-cell fusion during mating.
Fil: van Drogen, Frank. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Mishra, Ranjan. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Rudolf, Fabian. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Walczak, Michal J.. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Lee, Sung Sik. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Reiter, Wolfgang. Universidad de Viena; Austria
Fil: Hegemann, Björn. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Pelet, Serge. Universite de Lausanne; Suiza
Fil: Dohnal, Ilse. Universidad de Viena; Austria
Fil: Binolfi, Andrés. Leibniz-institut Für Molekulare Pharmakologie; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Yudina, Zinaida. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Selenko, Philipp. Leibniz-institut Für Molekulare Pharmakologie; Alemania
Fil: Wider, Gerhard. Eidgenössische Technische Hochschule Zürich; Suiza
Fil: Ammerer, Gustav. Universidad de Viena; Austria
Fil: Peter, Matthias. Eidgenössische Technische Hochschule Zürich; Suiza - Materia
-
PKC
STE5
NMR
MECHANICAL STRESS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/160854
Ver los metadatos del registro completo
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Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5van Drogen, FrankMishra, RanjanRudolf, FabianWalczak, Michal J.Lee, Sung SikReiter, WolfgangHegemann, BjörnPelet, SergeDohnal, IlseBinolfi, AndrésYudina, ZinaidaSelenko, PhilippWider, GerhardAmmerer, GustavPeter, MatthiasPKCSTE5NMRMECHANICAL STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mechanisms remain poorly understood. Here, we show that mechanical stress activates Pkc1, which prevents lysis of pheromone-treated cells by inhibiting polarized growth. In vitro Pkc1 phosphorylates conserved residues within the RING-H2 domains of the scaffold proteins Far1 and Ste5, which are also phosphorylated in vivo. Interestingly, Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3. Indeed, RING phosphorylation interferes with Ste5 membrane association by preventing binding to the receptor-linked Gβγ protein. Cells expressing nonphosphorylatable Ste5 undergo increased lysis upon mechanical stress and exhibit defects in cell-cell fusion during mating, which is exacerbated by simultaneous expression of nonphosphorylatable Far1. These results uncover a mechanical stress-triggered crosstalk mechanism modulating pheromone signaling, polarized growth, and cell-cell fusion during mating.Fil: van Drogen, Frank. Eidgenössische Technische Hochschule Zürich; SuizaFil: Mishra, Ranjan. Eidgenössische Technische Hochschule Zürich; SuizaFil: Rudolf, Fabian. Eidgenössische Technische Hochschule Zürich; SuizaFil: Walczak, Michal J.. Eidgenössische Technische Hochschule Zürich; SuizaFil: Lee, Sung Sik. Eidgenössische Technische Hochschule Zürich; SuizaFil: Reiter, Wolfgang. Universidad de Viena; AustriaFil: Hegemann, Björn. Eidgenössische Technische Hochschule Zürich; SuizaFil: Pelet, Serge. Universite de Lausanne; SuizaFil: Dohnal, Ilse. Universidad de Viena; AustriaFil: Binolfi, Andrés. Leibniz-institut Für Molekulare Pharmakologie; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Yudina, Zinaida. Eidgenössische Technische Hochschule Zürich; SuizaFil: Selenko, Philipp. Leibniz-institut Für Molekulare Pharmakologie; AlemaniaFil: Wider, Gerhard. Eidgenössische Technische Hochschule Zürich; SuizaFil: Ammerer, Gustav. Universidad de Viena; AustriaFil: Peter, Matthias. Eidgenössische Technische Hochschule Zürich; SuizaRockefeller University Press2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160854van Drogen, Frank; Mishra, Ranjan; Rudolf, Fabian; Walczak, Michal J.; Lee, Sung Sik; et al.; Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5; Rockefeller University Press; The Journal of cell biology; 218; 9; 9-2019; 3117-31331540-8140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://rupress.org/jcb/article-abstract/218/9/3117/120966/Mechanical-stress-impairs-pheromone-signaling-via?redirectedFrom=fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201808161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:15Zoai:ri.conicet.gov.ar:11336/160854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:15.68CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| title |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| spellingShingle |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 van Drogen, Frank PKC STE5 NMR MECHANICAL STRESS |
| title_short |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| title_full |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| title_fullStr |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| title_full_unstemmed |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| title_sort |
Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 |
| dc.creator.none.fl_str_mv |
van Drogen, Frank Mishra, Ranjan Rudolf, Fabian Walczak, Michal J. Lee, Sung Sik Reiter, Wolfgang Hegemann, Björn Pelet, Serge Dohnal, Ilse Binolfi, Andrés Yudina, Zinaida Selenko, Philipp Wider, Gerhard Ammerer, Gustav Peter, Matthias |
| author |
van Drogen, Frank |
| author_facet |
van Drogen, Frank Mishra, Ranjan Rudolf, Fabian Walczak, Michal J. Lee, Sung Sik Reiter, Wolfgang Hegemann, Björn Pelet, Serge Dohnal, Ilse Binolfi, Andrés Yudina, Zinaida Selenko, Philipp Wider, Gerhard Ammerer, Gustav Peter, Matthias |
| author_role |
author |
| author2 |
Mishra, Ranjan Rudolf, Fabian Walczak, Michal J. Lee, Sung Sik Reiter, Wolfgang Hegemann, Björn Pelet, Serge Dohnal, Ilse Binolfi, Andrés Yudina, Zinaida Selenko, Philipp Wider, Gerhard Ammerer, Gustav Peter, Matthias |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
PKC STE5 NMR MECHANICAL STRESS |
| topic |
PKC STE5 NMR MECHANICAL STRESS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mechanisms remain poorly understood. Here, we show that mechanical stress activates Pkc1, which prevents lysis of pheromone-treated cells by inhibiting polarized growth. In vitro Pkc1 phosphorylates conserved residues within the RING-H2 domains of the scaffold proteins Far1 and Ste5, which are also phosphorylated in vivo. Interestingly, Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3. Indeed, RING phosphorylation interferes with Ste5 membrane association by preventing binding to the receptor-linked Gβγ protein. Cells expressing nonphosphorylatable Ste5 undergo increased lysis upon mechanical stress and exhibit defects in cell-cell fusion during mating, which is exacerbated by simultaneous expression of nonphosphorylatable Far1. These results uncover a mechanical stress-triggered crosstalk mechanism modulating pheromone signaling, polarized growth, and cell-cell fusion during mating. Fil: van Drogen, Frank. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Mishra, Ranjan. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Rudolf, Fabian. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Walczak, Michal J.. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Lee, Sung Sik. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Reiter, Wolfgang. Universidad de Viena; Austria Fil: Hegemann, Björn. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Pelet, Serge. Universite de Lausanne; Suiza Fil: Dohnal, Ilse. Universidad de Viena; Austria Fil: Binolfi, Andrés. Leibniz-institut Für Molekulare Pharmakologie; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Yudina, Zinaida. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Selenko, Philipp. Leibniz-institut Für Molekulare Pharmakologie; Alemania Fil: Wider, Gerhard. Eidgenössische Technische Hochschule Zürich; Suiza Fil: Ammerer, Gustav. Universidad de Viena; Austria Fil: Peter, Matthias. Eidgenössische Technische Hochschule Zürich; Suiza |
| description |
Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mechanisms remain poorly understood. Here, we show that mechanical stress activates Pkc1, which prevents lysis of pheromone-treated cells by inhibiting polarized growth. In vitro Pkc1 phosphorylates conserved residues within the RING-H2 domains of the scaffold proteins Far1 and Ste5, which are also phosphorylated in vivo. Interestingly, Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3. Indeed, RING phosphorylation interferes with Ste5 membrane association by preventing binding to the receptor-linked Gβγ protein. Cells expressing nonphosphorylatable Ste5 undergo increased lysis upon mechanical stress and exhibit defects in cell-cell fusion during mating, which is exacerbated by simultaneous expression of nonphosphorylatable Far1. These results uncover a mechanical stress-triggered crosstalk mechanism modulating pheromone signaling, polarized growth, and cell-cell fusion during mating. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-09 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/160854 van Drogen, Frank; Mishra, Ranjan; Rudolf, Fabian; Walczak, Michal J.; Lee, Sung Sik; et al.; Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5; Rockefeller University Press; The Journal of cell biology; 218; 9; 9-2019; 3117-3133 1540-8140 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/160854 |
| identifier_str_mv |
van Drogen, Frank; Mishra, Ranjan; Rudolf, Fabian; Walczak, Michal J.; Lee, Sung Sik; et al.; Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5; Rockefeller University Press; The Journal of cell biology; 218; 9; 9-2019; 3117-3133 1540-8140 CONICET Digital CONICET |
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eng |
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eng |
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Rockefeller University Press |
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Rockefeller University Press |
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