Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases

Autores
Bouchet, Ana María; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
l-Arginine (Arg) is a positively charged amino acid constituent of peptides and proteins, participating in diverse mechanisms of protein-membrane interaction. The effect of Arg on phosphatidylcholine (PC) membranes has been previously related to water structure changes and to the presence of water defects in the hydrocarbon region. However, no information is available with regard to phosphatidylethanolamine (PE), another important component of lipid membranes. For this reason, the aim of this study is to determine the effect of Arg on DMPE membranes and partially methylated PEs in comparison to DMPC. The adsorption of the amino acid onto the lipid membranes was followed by determining the changes in the surface potential as a function of the bulk amino acid concentrations. The effects of Arg on the surface properties were also measured by changes in the surface pressure and the dipole potential. The onset of the transition temperature was measured with a fluorophore anchored at the membrane interphase. The results provide a new insight on amino acid-PE interactions, which can be ascribed to specific perturbations in the head group region induced by the guanidinium residue.
Fil: Bouchet, Ana María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lairion, Fabiana Norma. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
DIPOLE POTENTIAL
DMPC
DMPE
GUANIDINIUM GROUP
INTERFACIAL ANISOTROPY
L-ARGININE
LIPID MEMBRANE
SURFACE POTENTIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53547
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53547
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphasesBouchet, Ana MaríaLairion, Fabiana NormaDisalvo, Edgardo AnibalDIPOLE POTENTIALDMPCDMPEGUANIDINIUM GROUPINTERFACIAL ANISOTROPYL-ARGININELIPID MEMBRANESURFACE POTENTIALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1l-Arginine (Arg) is a positively charged amino acid constituent of peptides and proteins, participating in diverse mechanisms of protein-membrane interaction. The effect of Arg on phosphatidylcholine (PC) membranes has been previously related to water structure changes and to the presence of water defects in the hydrocarbon region. However, no information is available with regard to phosphatidylethanolamine (PE), another important component of lipid membranes. For this reason, the aim of this study is to determine the effect of Arg on DMPE membranes and partially methylated PEs in comparison to DMPC. The adsorption of the amino acid onto the lipid membranes was followed by determining the changes in the surface potential as a function of the bulk amino acid concentrations. The effects of Arg on the surface properties were also measured by changes in the surface pressure and the dipole potential. The onset of the transition temperature was measured with a fluorophore anchored at the membrane interphase. The results provide a new insight on amino acid-PE interactions, which can be ascribed to specific perturbations in the head group region induced by the guanidinium residue.Fil: Bouchet, Ana María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lairion, Fabiana Norma. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53547Bouchet, Ana María; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1798; 3; 3-2010; 616-6230005-2736CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273609003721info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2009.10.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:35Zoai:ri.conicet.gov.ar:11336/53547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:36.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
title Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
spellingShingle Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
Bouchet, Ana María
DIPOLE POTENTIAL
DMPC
DMPE
GUANIDINIUM GROUP
INTERFACIAL ANISOTROPY
L-ARGININE
LIPID MEMBRANE
SURFACE POTENTIAL
title_short Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
title_full Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
title_fullStr Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
title_full_unstemmed Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
title_sort Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases
dc.creator.none.fl_str_mv Bouchet, Ana María
Lairion, Fabiana Norma
Disalvo, Edgardo Anibal
author Bouchet, Ana María
author_facet Bouchet, Ana María
Lairion, Fabiana Norma
Disalvo, Edgardo Anibal
author_role author
author2 Lairion, Fabiana Norma
Disalvo, Edgardo Anibal
author2_role author
author
dc.subject.none.fl_str_mv DIPOLE POTENTIAL
DMPC
DMPE
GUANIDINIUM GROUP
INTERFACIAL ANISOTROPY
L-ARGININE
LIPID MEMBRANE
SURFACE POTENTIAL
topic DIPOLE POTENTIAL
DMPC
DMPE
GUANIDINIUM GROUP
INTERFACIAL ANISOTROPY
L-ARGININE
LIPID MEMBRANE
SURFACE POTENTIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv l-Arginine (Arg) is a positively charged amino acid constituent of peptides and proteins, participating in diverse mechanisms of protein-membrane interaction. The effect of Arg on phosphatidylcholine (PC) membranes has been previously related to water structure changes and to the presence of water defects in the hydrocarbon region. However, no information is available with regard to phosphatidylethanolamine (PE), another important component of lipid membranes. For this reason, the aim of this study is to determine the effect of Arg on DMPE membranes and partially methylated PEs in comparison to DMPC. The adsorption of the amino acid onto the lipid membranes was followed by determining the changes in the surface potential as a function of the bulk amino acid concentrations. The effects of Arg on the surface properties were also measured by changes in the surface pressure and the dipole potential. The onset of the transition temperature was measured with a fluorophore anchored at the membrane interphase. The results provide a new insight on amino acid-PE interactions, which can be ascribed to specific perturbations in the head group region induced by the guanidinium residue.
Fil: Bouchet, Ana María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lairion, Fabiana Norma. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description l-Arginine (Arg) is a positively charged amino acid constituent of peptides and proteins, participating in diverse mechanisms of protein-membrane interaction. The effect of Arg on phosphatidylcholine (PC) membranes has been previously related to water structure changes and to the presence of water defects in the hydrocarbon region. However, no information is available with regard to phosphatidylethanolamine (PE), another important component of lipid membranes. For this reason, the aim of this study is to determine the effect of Arg on DMPE membranes and partially methylated PEs in comparison to DMPC. The adsorption of the amino acid onto the lipid membranes was followed by determining the changes in the surface potential as a function of the bulk amino acid concentrations. The effects of Arg on the surface properties were also measured by changes in the surface pressure and the dipole potential. The onset of the transition temperature was measured with a fluorophore anchored at the membrane interphase. The results provide a new insight on amino acid-PE interactions, which can be ascribed to specific perturbations in the head group region induced by the guanidinium residue.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53547
Bouchet, Ana María; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1798; 3; 3-2010; 616-623
0005-2736
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53547
identifier_str_mv Bouchet, Ana María; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Role of guanidinium group in the insertion of l-arginine in DMPE and DMPC lipid interphases; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1798; 3; 3-2010; 616-623
0005-2736
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273609003721
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2009.10.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613976334270464
score 13.070432

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