Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity

Autores
Campelo, Adrián Esteban; Massheimer, Virginia Laura
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.
Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
Buenos Aires
Argentina
International Society of Endocrinology
Materia
ANDROGENS
OBESITY
OXIDATIVE STRESS
ANGIOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184400

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spelling Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesityCampelo, Adrián EstebanMassheimer, Virginia LauraANDROGENSOBESITYOXIDATIVE STRESSANGIOGENESIShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”Buenos AiresArgentinaInternational Society of EndocrinologyInternational Society of Endocrinology2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184400Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:37Zoai:ri.conicet.gov.ar:11336/184400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:37.793CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
title Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
spellingShingle Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
Campelo, Adrián Esteban
ANDROGENS
OBESITY
OXIDATIVE STRESS
ANGIOGENESIS
title_short Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
title_full Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
title_fullStr Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
title_full_unstemmed Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
title_sort Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
dc.creator.none.fl_str_mv Campelo, Adrián Esteban
Massheimer, Virginia Laura
author Campelo, Adrián Esteban
author_facet Campelo, Adrián Esteban
Massheimer, Virginia Laura
author_role author
author2 Massheimer, Virginia Laura
author2_role author
dc.subject.none.fl_str_mv ANDROGENS
OBESITY
OXIDATIVE STRESS
ANGIOGENESIS
topic ANDROGENS
OBESITY
OXIDATIVE STRESS
ANGIOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.
Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
Buenos Aires
Argentina
International Society of Endocrinology
description The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.
publishDate 2021
dc.date.none.fl_str_mv 2021
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status_str publishedVersion
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Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184400
identifier_str_mv Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1
CONICET Digital
CONICET
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