Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
- Autores
- Campelo, Adrián Esteban; Massheimer, Virginia Laura
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.
Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
Buenos Aires
Argentina
International Society of Endocrinology - Materia
-
ANDROGENS
OBESITY
OXIDATIVE STRESS
ANGIOGENESIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/184400
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Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesityCampelo, Adrián EstebanMassheimer, Virginia LauraANDROGENSOBESITYOXIDATIVE STRESSANGIOGENESIShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”Buenos AiresArgentinaInternational Society of EndocrinologyInternational Society of Endocrinology2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184400Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:37Zoai:ri.conicet.gov.ar:11336/184400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:37.793CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
title |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
spellingShingle |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity Campelo, Adrián Esteban ANDROGENS OBESITY OXIDATIVE STRESS ANGIOGENESIS |
title_short |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
title_full |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
title_fullStr |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
title_full_unstemmed |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
title_sort |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity |
dc.creator.none.fl_str_mv |
Campelo, Adrián Esteban Massheimer, Virginia Laura |
author |
Campelo, Adrián Esteban |
author_facet |
Campelo, Adrián Esteban Massheimer, Virginia Laura |
author_role |
author |
author2 |
Massheimer, Virginia Laura |
author2_role |
author |
dc.subject.none.fl_str_mv |
ANDROGENS OBESITY OXIDATIVE STRESS ANGIOGENESIS |
topic |
ANDROGENS OBESITY OXIDATIVE STRESS ANGIOGENESIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization. Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology” Buenos Aires Argentina International Society of Endocrinology |
description |
The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/184400 Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/184400 |
identifier_str_mv |
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/ |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
International Society of Endocrinology |
publisher.none.fl_str_mv |
International Society of Endocrinology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |