In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies

Autores
Bongiovani, Natalia Soledad; Popovich, Cecilia Angelines; Martinez, Ana Maria; Freije, Hugo; Constenla, Diana Teresita; Leonardi, Patricia Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of efficient, rapid and species-specific techniques is indispensable for assessing growth and neutral lipid yield in microalga cultures for biodiesel oil production. Nannochloropsis oculata is a small microalgae with a thick cell wall. In vivo techniques to estimate cell density, chlorophyll a and neutral lipids are reported. A calibration curve of cell density versus optical density was obtained and validated at 540 nm, under different growth phases. Intracellular neutral lipid storage was evaluated with fluorometry and epifluorescent microscopy employing fluorochrome Nile Red. The addition of 5% dimethyl sulfoxide enhanced 12.5 times the fluorescence signal efficiency. In situ fluorescence measurements allowed estimating the neutral lipid content (NR-FI). Besides, no significant differences were found in the lipid neutral content between gravimetric and triolein methods. The relationship between NR-FI and chlorophyll fluorescence signals was used as a neutral lipid accumulation index, which is useful in order to establish the optimum harvesting time. Thus, these procedures may be applied for a better monitoring mode of growth and neutral lipid accumulation in N. oculata's cultures at commercial scale.
Fil: Bongiovani, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
Fil: Martinez, Ana Maria. Universidad Nacional del Sur. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freije, Hugo. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
Materia
Chlorophyll A
Nannochloropsis Oculata
Neutral Lipids
Nile Red
Optical Density
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13503
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13503
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studiesBongiovani, Natalia SoledadPopovich, Cecilia AngelinesMartinez, Ana MariaFreije, HugoConstenla, Diana TeresitaLeonardi, Patricia InesChlorophyll ANannochloropsis OculataNeutral LipidsNile RedOptical Densityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The development of efficient, rapid and species-specific techniques is indispensable for assessing growth and neutral lipid yield in microalga cultures for biodiesel oil production. Nannochloropsis oculata is a small microalgae with a thick cell wall. In vivo techniques to estimate cell density, chlorophyll a and neutral lipids are reported. A calibration curve of cell density versus optical density was obtained and validated at 540 nm, under different growth phases. Intracellular neutral lipid storage was evaluated with fluorometry and epifluorescent microscopy employing fluorochrome Nile Red. The addition of 5% dimethyl sulfoxide enhanced 12.5 times the fluorescence signal efficiency. In situ fluorescence measurements allowed estimating the neutral lipid content (NR-FI). Besides, no significant differences were found in the lipid neutral content between gravimetric and triolein methods. The relationship between NR-FI and chlorophyll fluorescence signals was used as a neutral lipid accumulation index, which is useful in order to establish the optimum harvesting time. Thus, these procedures may be applied for a better monitoring mode of growth and neutral lipid accumulation in N. oculata's cultures at commercial scale.Fil: Bongiovani, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; ArgentinaFil: Martinez, Ana Maria. Universidad Nacional del Sur. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freije, Hugo. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; ArgentinaSchweizerbart Science Publishers2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.rarapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/13503Bongiovani, Natalia Soledad; Popovich, Cecilia Angelines; Martinez, Ana Maria; Freije, Hugo; Constenla, Diana Teresita; et al.; In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies; Schweizerbart Science Publishers; Algological Sudies; 142; 1; 5-2013; 3-161864-13182363-7080enginfo:eu-repo/semantics/altIdentifier/url/http://www.schweizerbart.de/papers/algol_stud/detail/142/80820/In_vivo_measurements_to_estimate_culture_status_and_neutral_lipid_accumulation_in_Nannochloropsis_oculata_CCALA_978_implications_for_biodiesel_oil_studiesinfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/schweiz/algo/2013/00000142/00000001/art00001info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1127/1864-1318/2013/0104info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:11Zoai:ri.conicet.gov.ar:11336/13503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:11.594CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
title In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
spellingShingle In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
Bongiovani, Natalia Soledad
Chlorophyll A
Nannochloropsis Oculata
Neutral Lipids
Nile Red
Optical Density
title_short In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
title_full In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
title_fullStr In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
title_full_unstemmed In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
title_sort In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies
dc.creator.none.fl_str_mv Bongiovani, Natalia Soledad
Popovich, Cecilia Angelines
Martinez, Ana Maria
Freije, Hugo
Constenla, Diana Teresita
Leonardi, Patricia Ines
author Bongiovani, Natalia Soledad
author_facet Bongiovani, Natalia Soledad
Popovich, Cecilia Angelines
Martinez, Ana Maria
Freije, Hugo
Constenla, Diana Teresita
Leonardi, Patricia Ines
author_role author
author2 Popovich, Cecilia Angelines
Martinez, Ana Maria
Freije, Hugo
Constenla, Diana Teresita
Leonardi, Patricia Ines
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chlorophyll A
Nannochloropsis Oculata
Neutral Lipids
Nile Red
Optical Density
topic Chlorophyll A
Nannochloropsis Oculata
Neutral Lipids
Nile Red
Optical Density
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of efficient, rapid and species-specific techniques is indispensable for assessing growth and neutral lipid yield in microalga cultures for biodiesel oil production. Nannochloropsis oculata is a small microalgae with a thick cell wall. In vivo techniques to estimate cell density, chlorophyll a and neutral lipids are reported. A calibration curve of cell density versus optical density was obtained and validated at 540 nm, under different growth phases. Intracellular neutral lipid storage was evaluated with fluorometry and epifluorescent microscopy employing fluorochrome Nile Red. The addition of 5% dimethyl sulfoxide enhanced 12.5 times the fluorescence signal efficiency. In situ fluorescence measurements allowed estimating the neutral lipid content (NR-FI). Besides, no significant differences were found in the lipid neutral content between gravimetric and triolein methods. The relationship between NR-FI and chlorophyll fluorescence signals was used as a neutral lipid accumulation index, which is useful in order to establish the optimum harvesting time. Thus, these procedures may be applied for a better monitoring mode of growth and neutral lipid accumulation in N. oculata's cultures at commercial scale.
Fil: Bongiovani, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
Fil: Martinez, Ana Maria. Universidad Nacional del Sur. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freije, Hugo. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
description The development of efficient, rapid and species-specific techniques is indispensable for assessing growth and neutral lipid yield in microalga cultures for biodiesel oil production. Nannochloropsis oculata is a small microalgae with a thick cell wall. In vivo techniques to estimate cell density, chlorophyll a and neutral lipids are reported. A calibration curve of cell density versus optical density was obtained and validated at 540 nm, under different growth phases. Intracellular neutral lipid storage was evaluated with fluorometry and epifluorescent microscopy employing fluorochrome Nile Red. The addition of 5% dimethyl sulfoxide enhanced 12.5 times the fluorescence signal efficiency. In situ fluorescence measurements allowed estimating the neutral lipid content (NR-FI). Besides, no significant differences were found in the lipid neutral content between gravimetric and triolein methods. The relationship between NR-FI and chlorophyll fluorescence signals was used as a neutral lipid accumulation index, which is useful in order to establish the optimum harvesting time. Thus, these procedures may be applied for a better monitoring mode of growth and neutral lipid accumulation in N. oculata's cultures at commercial scale.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13503
Bongiovani, Natalia Soledad; Popovich, Cecilia Angelines; Martinez, Ana Maria; Freije, Hugo; Constenla, Diana Teresita; et al.; In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies; Schweizerbart Science Publishers; Algological Sudies; 142; 1; 5-2013; 3-16
1864-1318
2363-7080
url http://hdl.handle.net/11336/13503
identifier_str_mv Bongiovani, Natalia Soledad; Popovich, Cecilia Angelines; Martinez, Ana Maria; Freije, Hugo; Constenla, Diana Teresita; et al.; In vivo measurements to estimate culture status and neutral lipid accumulation in Nannochloropsis oculata CCALA 978: implications for biodiesel oil studies; Schweizerbart Science Publishers; Algological Sudies; 142; 1; 5-2013; 3-16
1864-1318
2363-7080
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.schweizerbart.de/papers/algol_stud/detail/142/80820/In_vivo_measurements_to_estimate_culture_status_and_neutral_lipid_accumulation_in_Nannochloropsis_oculata_CCALA_978_implications_for_biodiesel_oil_studies
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/schweiz/algo/2013/00000142/00000001/art00001
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1127/1864-1318/2013/0104
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.rar
application/msword
application/pdf
dc.publisher.none.fl_str_mv Schweizerbart Science Publishers
publisher.none.fl_str_mv Schweizerbart Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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