Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression

Autores
Pezzoni, Magdalena; De Troch, Marleen; Pizarro, Ramón Augusto; Costa, Cristina Susana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In bacteria, exposure to changes in environmental conditions can alter membrane fluidity, thereby affecting its essential functions in cell physiology. To adapt to these changes, bacteria maintain appropriate fluidity by varying the composition of the fatty acids of membrane phospholipids, a phenomenon known as homeophasic adaptation. In Pseudomonas aeruginosa, this response is achieved mainly by two mechanisms of fatty acid desaturation: the FabA–FabB and DesA–DesB systems. This study analyzed the effect of ultraviolet-A (UVA) radiation—the major fraction of solar UV radiation reaching the Earth's surface—on the homeophasic process. The prototypical strain PAO1 was grown under sublethal UVA doses or in the dark, and the profiles of membrane fatty acids were compared at early logarithmic, logarithmic and stationary growth phases. In the logarithmic growth phase, it was observed that growth under sublethal UVA doses induced the expression of the desaturase-encoding genes desA and desB and increased the proportion of unsaturated fatty acids; in addition, membrane fluidity could also increase, as suggested by the indices used as indicators of this parameter. The opposite effect was observed in the stationary growth phase. These results demonstrate the relevant role of UVA on the homeophasic response at transcriptional level.
Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Troch, Marleen. University of Ghent; Bélgica
Fil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica; Argentina
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica; Argentina
Materia
RADIACIÓN ULTRAVIOLETA A
HOMEOPHASIC ADAPTATION
PSEUDOMONAS AERUGINOSA
FATTY ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196864
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB ExpressionPezzoni, MagdalenaDe Troch, MarleenPizarro, Ramón AugustoCosta, Cristina SusanaRADIACIÓN ULTRAVIOLETA AHOMEOPHASIC ADAPTATIONPSEUDOMONAS AERUGINOSAFATTY ACIDhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1In bacteria, exposure to changes in environmental conditions can alter membrane fluidity, thereby affecting its essential functions in cell physiology. To adapt to these changes, bacteria maintain appropriate fluidity by varying the composition of the fatty acids of membrane phospholipids, a phenomenon known as homeophasic adaptation. In Pseudomonas aeruginosa, this response is achieved mainly by two mechanisms of fatty acid desaturation: the FabA–FabB and DesA–DesB systems. This study analyzed the effect of ultraviolet-A (UVA) radiation—the major fraction of solar UV radiation reaching the Earth's surface—on the homeophasic process. The prototypical strain PAO1 was grown under sublethal UVA doses or in the dark, and the profiles of membrane fatty acids were compared at early logarithmic, logarithmic and stationary growth phases. In the logarithmic growth phase, it was observed that growth under sublethal UVA doses induced the expression of the desaturase-encoding genes desA and desB and increased the proportion of unsaturated fatty acids; in addition, membrane fluidity could also increase, as suggested by the indices used as indicators of this parameter. The opposite effect was observed in the stationary growth phase. These results demonstrate the relevant role of UVA on the homeophasic response at transcriptional level.Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Troch, Marleen. University of Ghent; BélgicaFil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica; ArgentinaFil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica; ArgentinaWiley Blackwell Publishing, Inc2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196864Pezzoni, Magdalena; De Troch, Marleen; Pizarro, Ramón Augusto; Costa, Cristina Susana; Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 98; 4; 7-2022; 886-8930031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/php.13548info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:32Zoai:ri.conicet.gov.ar:11336/196864instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:33.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
title Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
spellingShingle Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
Pezzoni, Magdalena
RADIACIÓN ULTRAVIOLETA A
HOMEOPHASIC ADAPTATION
PSEUDOMONAS AERUGINOSA
FATTY ACID
title_short Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
title_full Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
title_fullStr Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
title_full_unstemmed Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
title_sort Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression
dc.creator.none.fl_str_mv Pezzoni, Magdalena
De Troch, Marleen
Pizarro, Ramón Augusto
Costa, Cristina Susana
author Pezzoni, Magdalena
author_facet Pezzoni, Magdalena
De Troch, Marleen
Pizarro, Ramón Augusto
Costa, Cristina Susana
author_role author
author2 De Troch, Marleen
Pizarro, Ramón Augusto
Costa, Cristina Susana
author2_role author
author
author
dc.subject.none.fl_str_mv RADIACIÓN ULTRAVIOLETA A
HOMEOPHASIC ADAPTATION
PSEUDOMONAS AERUGINOSA
FATTY ACID
topic RADIACIÓN ULTRAVIOLETA A
HOMEOPHASIC ADAPTATION
PSEUDOMONAS AERUGINOSA
FATTY ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In bacteria, exposure to changes in environmental conditions can alter membrane fluidity, thereby affecting its essential functions in cell physiology. To adapt to these changes, bacteria maintain appropriate fluidity by varying the composition of the fatty acids of membrane phospholipids, a phenomenon known as homeophasic adaptation. In Pseudomonas aeruginosa, this response is achieved mainly by two mechanisms of fatty acid desaturation: the FabA–FabB and DesA–DesB systems. This study analyzed the effect of ultraviolet-A (UVA) radiation—the major fraction of solar UV radiation reaching the Earth's surface—on the homeophasic process. The prototypical strain PAO1 was grown under sublethal UVA doses or in the dark, and the profiles of membrane fatty acids were compared at early logarithmic, logarithmic and stationary growth phases. In the logarithmic growth phase, it was observed that growth under sublethal UVA doses induced the expression of the desaturase-encoding genes desA and desB and increased the proportion of unsaturated fatty acids; in addition, membrane fluidity could also increase, as suggested by the indices used as indicators of this parameter. The opposite effect was observed in the stationary growth phase. These results demonstrate the relevant role of UVA on the homeophasic response at transcriptional level.
Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Troch, Marleen. University of Ghent; Bélgica
Fil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica; Argentina
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica; Argentina
description In bacteria, exposure to changes in environmental conditions can alter membrane fluidity, thereby affecting its essential functions in cell physiology. To adapt to these changes, bacteria maintain appropriate fluidity by varying the composition of the fatty acids of membrane phospholipids, a phenomenon known as homeophasic adaptation. In Pseudomonas aeruginosa, this response is achieved mainly by two mechanisms of fatty acid desaturation: the FabA–FabB and DesA–DesB systems. This study analyzed the effect of ultraviolet-A (UVA) radiation—the major fraction of solar UV radiation reaching the Earth's surface—on the homeophasic process. The prototypical strain PAO1 was grown under sublethal UVA doses or in the dark, and the profiles of membrane fatty acids were compared at early logarithmic, logarithmic and stationary growth phases. In the logarithmic growth phase, it was observed that growth under sublethal UVA doses induced the expression of the desaturase-encoding genes desA and desB and increased the proportion of unsaturated fatty acids; in addition, membrane fluidity could also increase, as suggested by the indices used as indicators of this parameter. The opposite effect was observed in the stationary growth phase. These results demonstrate the relevant role of UVA on the homeophasic response at transcriptional level.
publishDate 2022
dc.date.none.fl_str_mv 2022-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196864
Pezzoni, Magdalena; De Troch, Marleen; Pizarro, Ramón Augusto; Costa, Cristina Susana; Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 98; 4; 7-2022; 886-893
0031-8655
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196864
identifier_str_mv Pezzoni, Magdalena; De Troch, Marleen; Pizarro, Ramón Augusto; Costa, Cristina Susana; Homeophasic Adaptation in Response to UVA Radiation in Pseudomonas aeruginosa: Changes of Membrane Fatty Acid Composition and Induction of desA and desB Expression; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 98; 4; 7-2022; 886-893
0031-8655
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/php.13548
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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