Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthon...

Autores: Aschemacher, Nicolás Ariel; Teglia, Carla Mariela; Siano, Alvaro Sebastían; Gutierrez, Fabiana Andrea; Goicoechea, Hector Casimiro
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The present work describes for the first time the use of a hydrophobic natural deep eutectic solvent (H-NADES) as a dispersant for carboxylated nanotubes for the design and construction of an electrochemical sensor for the assay of α-glucosidase and its inhibitors. In this work, we used as the electrochemical probe the product of the enzymatic reaction, which consists of two redox groups and generates the analytical signal. The combination of de carboxylic multi-walled carbon nanotubes (MWCNTc) and the H-NADES of thymol and lactic acid (TLa) increases the electroactive surface area and promotes electron transfer of the electrode modified with carbon nanotubes. The electrochemical sensor enabled the detection of α-glucosidase in a range of 0.004–0.1 U mL−1 with a detection limit of 0.0013 U mL−1, which is lower than most existing methods. In addition, two α-glucosidase inhibitors, acarbose and quercetin, and two plant extracts, Schinus molle and Eugenia uniflora, were evaluated to assess the feasibility of screening potential antidiabetic drugs, and the IC50 values were 5.37 μg mL−1 and 5.28 μg mL−1. Thus, this sensing strategy represents the beginning of the incorporation of NADES in the development and design of novel sensors and their application in electrochemistry and medical analysis.
Fil: Aschemacher, Nicolás Ariel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina
Fil: Teglia, Carla Mariela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Materia: CARBON NANOTUBES
ELECTROCHEMICAL SENSORS
H-NADES
MEDICINAL PLANTS
RESPONSE SURFACE METHODOLOGY
Α-GLUCOSIDASE
Nivel de accesibilidad: acceso embargado
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/231165

Acceder

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plantsAschemacher, Nicolás ArielTeglia, Carla MarielaSiano, Alvaro SebastíanGutierrez, Fabiana AndreaGoicoechea, Hector CasimiroCARBON NANOTUBESELECTROCHEMICAL SENSORSH-NADESMEDICINAL PLANTSRESPONSE SURFACE METHODOLOGYΑ-GLUCOSIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The present work describes for the first time the use of a hydrophobic natural deep eutectic solvent (H-NADES) as a dispersant for carboxylated nanotubes for the design and construction of an electrochemical sensor for the assay of α-glucosidase and its inhibitors. In this work, we used as the electrochemical probe the product of the enzymatic reaction, which consists of two redox groups and generates the analytical signal. The combination of de carboxylic multi-walled carbon nanotubes (MWCNTc) and the H-NADES of thymol and lactic acid (TLa) increases the electroactive surface area and promotes electron transfer of the electrode modified with carbon nanotubes. The electrochemical sensor enabled the detection of α-glucosidase in a range of 0.004–0.1 U mL−1 with a detection limit of 0.0013 U mL−1, which is lower than most existing methods. In addition, two α-glucosidase inhibitors, acarbose and quercetin, and two plant extracts, Schinus molle and Eugenia uniflora, were evaluated to assess the feasibility of screening potential antidiabetic drugs, and the IC50 values were 5.37 μg mL−1 and 5.28 μg mL−1. Thus, this sensing strategy represents the beginning of the incorporation of NADES in the development and design of novel sensors and their application in electrochemistry and medical analysis.Fil: Aschemacher, Nicolás Ariel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; ArgentinaFil: Teglia, Carla Mariela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaElsevier Science2024-02info:eu-repo/date/embargoEnd/2024-08-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231165Aschemacher, Nicolás Ariel; Teglia, Carla Mariela; Siano, Alvaro Sebastían; Gutierrez, Fabiana Andrea; Goicoechea, Hector Casimiro; Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants; Elsevier Science; Talanta; 268; 2-2024; 1-380039-9140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0039914023010640info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2023.125313info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:36:54Zoai:ri.conicet.gov.ar:11336/231165instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:36:54.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
title	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
spellingShingle	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants Aschemacher, Nicolás Ariel CARBON NANOTUBES ELECTROCHEMICAL SENSORS H-NADES MEDICINAL PLANTS RESPONSE SURFACE METHODOLOGY Α-GLUCOSIDASE
title_short	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
title_full	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
title_fullStr	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
title_full_unstemmed	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
title_sort	Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants
dc.creator.none.fl_str_mv	Aschemacher, Nicolás Ariel Teglia, Carla Mariela Siano, Alvaro Sebastían Gutierrez, Fabiana Andrea Goicoechea, Hector Casimiro
author	Aschemacher, Nicolás Ariel
author_facet	Aschemacher, Nicolás Ariel Teglia, Carla Mariela Siano, Alvaro Sebastían Gutierrez, Fabiana Andrea Goicoechea, Hector Casimiro
author_role	author
author2	Teglia, Carla Mariela Siano, Alvaro Sebastían Gutierrez, Fabiana Andrea Goicoechea, Hector Casimiro
author2_role	author author author author
dc.subject.none.fl_str_mv	CARBON NANOTUBES ELECTROCHEMICAL SENSORS H-NADES MEDICINAL PLANTS RESPONSE SURFACE METHODOLOGY Α-GLUCOSIDASE
topic	CARBON NANOTUBES ELECTROCHEMICAL SENSORS H-NADES MEDICINAL PLANTS RESPONSE SURFACE METHODOLOGY Α-GLUCOSIDASE
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	The present work describes for the first time the use of a hydrophobic natural deep eutectic solvent (H-NADES) as a dispersant for carboxylated nanotubes for the design and construction of an electrochemical sensor for the assay of α-glucosidase and its inhibitors. In this work, we used as the electrochemical probe the product of the enzymatic reaction, which consists of two redox groups and generates the analytical signal. The combination of de carboxylic multi-walled carbon nanotubes (MWCNTc) and the H-NADES of thymol and lactic acid (TLa) increases the electroactive surface area and promotes electron transfer of the electrode modified with carbon nanotubes. The electrochemical sensor enabled the detection of α-glucosidase in a range of 0.004–0.1 U mL−1 with a detection limit of 0.0013 U mL−1, which is lower than most existing methods. In addition, two α-glucosidase inhibitors, acarbose and quercetin, and two plant extracts, Schinus molle and Eugenia uniflora, were evaluated to assess the feasibility of screening potential antidiabetic drugs, and the IC50 values were 5.37 μg mL−1 and 5.28 μg mL−1. Thus, this sensing strategy represents the beginning of the incorporation of NADES in the development and design of novel sensors and their application in electrochemistry and medical analysis. Fil: Aschemacher, Nicolás Ariel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina Fil: Teglia, Carla Mariela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Siano, Alvaro Sebastían. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química Organica. Laboratorio de Peptidos Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
description	The present work describes for the first time the use of a hydrophobic natural deep eutectic solvent (H-NADES) as a dispersant for carboxylated nanotubes for the design and construction of an electrochemical sensor for the assay of α-glucosidase and its inhibitors. In this work, we used as the electrochemical probe the product of the enzymatic reaction, which consists of two redox groups and generates the analytical signal. The combination of de carboxylic multi-walled carbon nanotubes (MWCNTc) and the H-NADES of thymol and lactic acid (TLa) increases the electroactive surface area and promotes electron transfer of the electrode modified with carbon nanotubes. The electrochemical sensor enabled the detection of α-glucosidase in a range of 0.004–0.1 U mL−1 with a detection limit of 0.0013 U mL−1, which is lower than most existing methods. In addition, two α-glucosidase inhibitors, acarbose and quercetin, and two plant extracts, Schinus molle and Eugenia uniflora, were evaluated to assess the feasibility of screening potential antidiabetic drugs, and the IC50 values were 5.37 μg mL−1 and 5.28 μg mL−1. Thus, this sensing strategy represents the beginning of the incorporation of NADES in the development and design of novel sensors and their application in electrochemistry and medical analysis.
publishDate	2024
dc.date.none.fl_str_mv	2024-02 info:eu-repo/date/embargoEnd/2024-08-21
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/231165 Aschemacher, Nicolás Ariel; Teglia, Carla Mariela; Siano, Alvaro Sebastían; Gutierrez, Fabiana Andrea; Goicoechea, Hector Casimiro; Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants; Elsevier Science; Talanta; 268; 2-2024; 1-38 0039-9140 CONICET Digital CONICET
url	http://hdl.handle.net/11336/231165
identifier_str_mv	Aschemacher, Nicolás Ariel; Teglia, Carla Mariela; Siano, Alvaro Sebastían; Gutierrez, Fabiana Andrea; Goicoechea, Hector Casimiro; Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthonous medicinal plants; Elsevier Science; Talanta; 268; 2-2024; 1-38 0039-9140 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0039914023010640 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2023.125313
dc.rights.none.fl_str_mv	info:eu-repo/semantics/embargoedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	embargoedAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier Science
publisher.none.fl_str_mv	Elsevier Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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Development of an electrochemical sensor using carbon nanotubes and hydrophobic natural deep eutectic solvents for the detection of α-glucosidase activity in extracts of autochthon...

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