The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes

Autores
Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio; Hildebrandt, Peter; Bauer, Christian; Pereira, Manuela M.; Kletzin, Arnulf; Teixeira, Miguel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes. These genes are part of the pentacistronic cbsAB-soxLN-odsN locus. The spectroscopic characterization revealed the presence of three low-spin hemes, two of the b and one of the as-type with reduction potentials of + 200, + 400 and + 160 mV, respectively. The SoxN protein is proposed to harbor the heme b of lower reduction potential and the heme as, and CbsA the other heme b. The soxL gene encodes a Rieske protein, which was expressed in E. coli; its reduction potential was determined to be + 320 mV. Topology predictions showed that SoxN, CbsB and CbsA should contain 12, 9 and one transmembrane α-helices, respectively, with SoxN having a predicted fold very similar to those of the cytochromes b in bc1 complexes. The presence of two quinol binding motifs was also predicted in SoxN. Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc1 complexes of bacteria and mitochondria, however with distinct subunits and heme types.
Fil: Bandeiras, Tiago M.. Universidade Nova de Lisboa; Portugal
Fil: Refojo, Patricia N.. Universidade Nova de Lisboa; Portugal
Fil: Todorovic, Smilja. Universidade Nova de Lisboa; Portugal
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Technishe Universitat Berlin; Alemania
Fil: Hildebrandt, Peter. Technishe Universitat Berlin; Alemania
Fil: Bauer, Christian. Darmstadt University of Technology; Alemania
Fil: Pereira, Manuela M.. Universidade Nova de Lisboa; Portugal
Fil: Kletzin, Arnulf. Darmstadt University of Technology; Alemania
Fil: Teixeira, Miguel. Universidade Nova de Lisboa; Portugal
Materia
Archaea
B6f Complex
Bc1 Complex
Cytochrome B
Rieske
Sulfolobales
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/83606
Acceder
id CONICETDig_c9eb55aee356f363f027626f7b75c69d
oai_identifier_str oai:ri.conicet.gov.ar:11336/83606
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexesBandeiras, Tiago M.Refojo, Patricia N.Todorovic, SmiljaMurgida, Daniel HoracioHildebrandt, PeterBauer, ChristianPereira, Manuela M.Kletzin, ArnulfTeixeira, MiguelArchaeaB6f ComplexBc1 ComplexCytochrome BRieskeSulfolobaleshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes. These genes are part of the pentacistronic cbsAB-soxLN-odsN locus. The spectroscopic characterization revealed the presence of three low-spin hemes, two of the b and one of the as-type with reduction potentials of + 200, + 400 and + 160 mV, respectively. The SoxN protein is proposed to harbor the heme b of lower reduction potential and the heme as, and CbsA the other heme b. The soxL gene encodes a Rieske protein, which was expressed in E. coli; its reduction potential was determined to be + 320 mV. Topology predictions showed that SoxN, CbsB and CbsA should contain 12, 9 and one transmembrane α-helices, respectively, with SoxN having a predicted fold very similar to those of the cytochromes b in bc1 complexes. The presence of two quinol binding motifs was also predicted in SoxN. Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc1 complexes of bacteria and mitochondria, however with distinct subunits and heme types.Fil: Bandeiras, Tiago M.. Universidade Nova de Lisboa; PortugalFil: Refojo, Patricia N.. Universidade Nova de Lisboa; PortugalFil: Todorovic, Smilja. Universidade Nova de Lisboa; PortugalFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Technishe Universitat Berlin; AlemaniaFil: Hildebrandt, Peter. Technishe Universitat Berlin; AlemaniaFil: Bauer, Christian. Darmstadt University of Technology; AlemaniaFil: Pereira, Manuela M.. Universidade Nova de Lisboa; PortugalFil: Kletzin, Arnulf. Darmstadt University of Technology; AlemaniaFil: Teixeira, Miguel. Universidade Nova de Lisboa; PortugalElsevier Science2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/83606Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio; Hildebrandt, Peter; et al.; The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1787; 1; 1-2009; 37-450005-2728CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272808006865info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbabio.2008.09.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:08Zoai:ri.conicet.gov.ar:11336/83606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:08.826CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
title The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
spellingShingle The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
Bandeiras, Tiago M.
Archaea
B6f Complex
Bc1 Complex
Cytochrome B
Rieske
Sulfolobales
title_short The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
title_full The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
title_fullStr The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
title_full_unstemmed The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
title_sort The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
dc.creator.none.fl_str_mv Bandeiras, Tiago M.
Refojo, Patricia N.
Todorovic, Smilja
Murgida, Daniel Horacio
Hildebrandt, Peter
Bauer, Christian
Pereira, Manuela M.
Kletzin, Arnulf
Teixeira, Miguel
author Bandeiras, Tiago M.
author_facet Bandeiras, Tiago M.
Refojo, Patricia N.
Todorovic, Smilja
Murgida, Daniel Horacio
Hildebrandt, Peter
Bauer, Christian
Pereira, Manuela M.
Kletzin, Arnulf
Teixeira, Miguel
author_role author
author2 Refojo, Patricia N.
Todorovic, Smilja
Murgida, Daniel Horacio
Hildebrandt, Peter
Bauer, Christian
Pereira, Manuela M.
Kletzin, Arnulf
Teixeira, Miguel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Archaea
B6f Complex
Bc1 Complex
Cytochrome B
Rieske
Sulfolobales
topic Archaea
B6f Complex
Bc1 Complex
Cytochrome B
Rieske
Sulfolobales
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes. These genes are part of the pentacistronic cbsAB-soxLN-odsN locus. The spectroscopic characterization revealed the presence of three low-spin hemes, two of the b and one of the as-type with reduction potentials of + 200, + 400 and + 160 mV, respectively. The SoxN protein is proposed to harbor the heme b of lower reduction potential and the heme as, and CbsA the other heme b. The soxL gene encodes a Rieske protein, which was expressed in E. coli; its reduction potential was determined to be + 320 mV. Topology predictions showed that SoxN, CbsB and CbsA should contain 12, 9 and one transmembrane α-helices, respectively, with SoxN having a predicted fold very similar to those of the cytochromes b in bc1 complexes. The presence of two quinol binding motifs was also predicted in SoxN. Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc1 complexes of bacteria and mitochondria, however with distinct subunits and heme types.
Fil: Bandeiras, Tiago M.. Universidade Nova de Lisboa; Portugal
Fil: Refojo, Patricia N.. Universidade Nova de Lisboa; Portugal
Fil: Todorovic, Smilja. Universidade Nova de Lisboa; Portugal
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Technishe Universitat Berlin; Alemania
Fil: Hildebrandt, Peter. Technishe Universitat Berlin; Alemania
Fil: Bauer, Christian. Darmstadt University of Technology; Alemania
Fil: Pereira, Manuela M.. Universidade Nova de Lisboa; Portugal
Fil: Kletzin, Arnulf. Darmstadt University of Technology; Alemania
Fil: Teixeira, Miguel. Universidade Nova de Lisboa; Portugal
description A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes. These genes are part of the pentacistronic cbsAB-soxLN-odsN locus. The spectroscopic characterization revealed the presence of three low-spin hemes, two of the b and one of the as-type with reduction potentials of + 200, + 400 and + 160 mV, respectively. The SoxN protein is proposed to harbor the heme b of lower reduction potential and the heme as, and CbsA the other heme b. The soxL gene encodes a Rieske protein, which was expressed in E. coli; its reduction potential was determined to be + 320 mV. Topology predictions showed that SoxN, CbsB and CbsA should contain 12, 9 and one transmembrane α-helices, respectively, with SoxN having a predicted fold very similar to those of the cytochromes b in bc1 complexes. The presence of two quinol binding motifs was also predicted in SoxN. Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc1 complexes of bacteria and mitochondria, however with distinct subunits and heme types.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/83606
Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio; Hildebrandt, Peter; et al.; The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1787; 1; 1-2009; 37-45
0005-2728
CONICET Digital
CONICET
url http://hdl.handle.net/11336/83606
identifier_str_mv Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio; Hildebrandt, Peter; et al.; The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1787; 1; 1-2009; 37-45
0005-2728
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272808006865
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbabio.2008.09.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614478355759104
score 13.070432

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