The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence

Autores
Ruíz-Ranwez, Verónica; Posadas, Diana Maria; Estein, Silvia Marcela; Abdian, Patricia Lorena; Martin, Fernando Ariel; Zorreguieta, Angeles
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract
The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and BtaE adhesins, which belong to the monomeric and trimeric autotransporter (TA) families, respectively. It was previously shown that these adhesins are involved in bacterial adhesion to
host cells and components of the extracellular matrix (ECM). In this work we describe the role of a new member of the TA family of B. suis (named BtaF) in the adhesive properties of the bacterial surface. BtaF conferred the bacteria that carried it a promiscuous adhesiveness to various ECM components and the ability to attach to an abiotic surface. Furthermore, BtaF was found to participate in bacterial adhesion to epithelial cells and was required for full virulence in mice. Similar to BmaC and BtaE, the BtaF adhesin was expressed in a small subpopulation of bacteria, and in all cases, it was detected at the new  pole generated after cell division. Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle.
Fil: Ruíz Ranwez V. FUND.INSTITUTO LELOIR.
Fil: Posadas Dm,. FUND.INSTITUTO LELOIR.
Fil: Estein S.m.. UNIV.NAC.DEL CENTRO DE LA PCIA.DE BS.AS.. FAC.DE CS.VETERINARIAS. DTO.DE SANIDAD ANIMAL Y MEDICINA PREVENTIVA.
Fil: Abdian P.l.. FUND.INSTITUTO LELOIR.
Fil: Martín F.a. FUND.INSTITUTO LELOIR.
Fil: Zorreguieta A.. FUND.INSTITUTO LELOIR.
Materia
BTAF
AUTOTRANSPORTER
BRUCELLA SUIS
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/501
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/501
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and VirulenceRuíz-Ranwez, VerónicaPosadas, Diana MariaEstein, Silvia MarcelaAbdian, Patricia LorenaMartin, Fernando ArielZorreguieta, AngelesBTAFAUTOTRANSPORTERBRUCELLA SUISVIRULENCEhttps://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.3Abstract<br />The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and BtaE adhesins, which belong to the monomeric and trimeric autotransporter (TA) families, respectively. It was previously shown that these adhesins are involved in bacterial adhesion to<br />host cells and components of the extracellular matrix (ECM). In this work we describe the role of a new member of the TA family of B. suis (named BtaF) in the adhesive properties of the bacterial surface. BtaF conferred the bacteria that carried it a promiscuous adhesiveness to various ECM components and the ability to attach to an abiotic surface. Furthermore, BtaF was found to participate in bacterial adhesion to epithelial cells and was required for full virulence in mice. Similar to BmaC and BtaE, the BtaF adhesin was expressed in a small subpopulation of bacteria, and in all cases, it was detected at the new  pole generated after cell division. Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle.Fil: Ruíz Ranwez V. FUND.INSTITUTO LELOIR.Fil: Posadas Dm,. FUND.INSTITUTO LELOIR.Fil: Estein S.m.. UNIV.NAC.DEL CENTRO DE LA PCIA.DE BS.AS.. FAC.DE CS.VETERINARIAS. DTO.DE SANIDAD ANIMAL Y MEDICINA PREVENTIVA.Fil: Abdian P.l.. FUND.INSTITUTO LELOIR.Fil: Martín F.a. FUND.INSTITUTO LELOIR.Fil: Zorreguieta A.. FUND.INSTITUTO LELOIR.Public Library Science2013-11-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/501Ruíz-Ranwez, Verónica; Posadas, Diana Maria; Estein, Silvia Marcela; Abdian, Patricia Lorena; Martin, Fernando Ariel; Zorreguieta, Angeles; The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence ; Public Library Science; Plos One; 8; 11; 13-11-2013; 1-13;1932-620310.1371/journal.pone.0079770enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0079770info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:39Zoai:ri.conicet.gov.ar:11336/501instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:39.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
title The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
spellingShingle The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
Ruíz-Ranwez, Verónica
BTAF
AUTOTRANSPORTER
BRUCELLA SUIS
VIRULENCE
title_short The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
title_full The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
title_fullStr The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
title_full_unstemmed The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
title_sort The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence
dc.creator.none.fl_str_mv Ruíz-Ranwez, Verónica
Posadas, Diana Maria
Estein, Silvia Marcela
Abdian, Patricia Lorena
Martin, Fernando Ariel
Zorreguieta, Angeles
author Ruíz-Ranwez, Verónica
author_facet Ruíz-Ranwez, Verónica
Posadas, Diana Maria
Estein, Silvia Marcela
Abdian, Patricia Lorena
Martin, Fernando Ariel
Zorreguieta, Angeles
author_role author
author2 Posadas, Diana Maria
Estein, Silvia Marcela
Abdian, Patricia Lorena
Martin, Fernando Ariel
Zorreguieta, Angeles
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BTAF
AUTOTRANSPORTER
BRUCELLA SUIS
VIRULENCE
topic BTAF
AUTOTRANSPORTER
BRUCELLA SUIS
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.3
dc.description.none.fl_txt_mv Abstract<br />The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and BtaE adhesins, which belong to the monomeric and trimeric autotransporter (TA) families, respectively. It was previously shown that these adhesins are involved in bacterial adhesion to<br />host cells and components of the extracellular matrix (ECM). In this work we describe the role of a new member of the TA family of B. suis (named BtaF) in the adhesive properties of the bacterial surface. BtaF conferred the bacteria that carried it a promiscuous adhesiveness to various ECM components and the ability to attach to an abiotic surface. Furthermore, BtaF was found to participate in bacterial adhesion to epithelial cells and was required for full virulence in mice. Similar to BmaC and BtaE, the BtaF adhesin was expressed in a small subpopulation of bacteria, and in all cases, it was detected at the new  pole generated after cell division. Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle.
Fil: Ruíz Ranwez V. FUND.INSTITUTO LELOIR.
Fil: Posadas Dm,. FUND.INSTITUTO LELOIR.
Fil: Estein S.m.. UNIV.NAC.DEL CENTRO DE LA PCIA.DE BS.AS.. FAC.DE CS.VETERINARIAS. DTO.DE SANIDAD ANIMAL Y MEDICINA PREVENTIVA.
Fil: Abdian P.l.. FUND.INSTITUTO LELOIR.
Fil: Martín F.a. FUND.INSTITUTO LELOIR.
Fil: Zorreguieta A.. FUND.INSTITUTO LELOIR.
description Abstract<br />The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and BtaE adhesins, which belong to the monomeric and trimeric autotransporter (TA) families, respectively. It was previously shown that these adhesins are involved in bacterial adhesion to<br />host cells and components of the extracellular matrix (ECM). In this work we describe the role of a new member of the TA family of B. suis (named BtaF) in the adhesive properties of the bacterial surface. BtaF conferred the bacteria that carried it a promiscuous adhesiveness to various ECM components and the ability to attach to an abiotic surface. Furthermore, BtaF was found to participate in bacterial adhesion to epithelial cells and was required for full virulence in mice. Similar to BmaC and BtaE, the BtaF adhesin was expressed in a small subpopulation of bacteria, and in all cases, it was detected at the new  pole generated after cell division. Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-13
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
status_str publishedVersion
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/501
Ruíz-Ranwez, Verónica; Posadas, Diana Maria; Estein, Silvia Marcela; Abdian, Patricia Lorena; Martin, Fernando Ariel; Zorreguieta, Angeles; The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence ; Public Library Science; Plos One; 8; 11; 13-11-2013; 1-13;
1932-6203
10.1371/journal.pone.0079770
url http://hdl.handle.net/11336/501
identifier_str_mv Ruíz-Ranwez, Verónica; Posadas, Diana Maria; Estein, Silvia Marcela; Abdian, Patricia Lorena; Martin, Fernando Ariel; Zorreguieta, Angeles; The BtaF Trimeric Autotransporter of Brucella suis Is Involved in Attachment to Various Surfaces, Resistance to Serum and Virulence ; Public Library Science; Plos One; 8; 11; 13-11-2013; 1-13;
1932-6203
10.1371/journal.pone.0079770
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0079770
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082866738364416
score 13.22299

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