Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats

Autores
Pietranera, Luciana; Brocca, María Elvira; Cymeryng, Cora Betriz; Gomez Sanchez, E.; Gomez Sanchez, C. E.; Roig, Paulina; Lima, Analia Ethel; de Nicola, Alejandro Federico
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mineralocorticoid receptor (MR) has been considered both neuroprotective as well as damaging for central nervous system function. MR may be also involved in central regulation of blood pressure. In the present study we compared the expression of MR and the glucocorticoid receptor (GR) in the hippocampus and hypothalamus of 16 weeks old spontaneously hypertensive rats (SHR) and the normotensive control WKY rats. In hippocampus, MR expression was studied by in situ hybridization (ISH), qPCR, and immunohistochemistry, whereas GR expression was analyzed by the last two procedures. Hypertensive animals showed increased expression of MR mRNA in whole hippocampus according to qPCR data and also in CA3 by ISH. Immunocytochemical staining for MR of dorsal hippocampus, however, did not show differences between SHR and WKY. SHR showed elevated hypothalamic MR mRNA by qPCR, and increased number of MR immunopositive cells in the magnocellular paraventricular region, compared to WKY. In contrast, expression levels of GR mRNA or protein in hippocampus and hypothalamus of SHR were similar to those of WKY rats. Furthermore, we further investigated the role of MR in the hypertensive rats by intracerebroventricular injection of the MR antagonist RU-2831 This compound produced a significant drop in blood pressure of SHR. In conclusion, MR expression is increased in the hippocampus and hypothalamus of SHR. We suggest that pathological MR overdrive may take responsibility for up regulation of blood pressure and the encephalopathy of hypertension.
Fil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Brocca, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cymeryng, Cora Betriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Gomez Sanchez, E.. Mississippi State University; Estados Unidos
Fil: Gomez Sanchez, C. E.. Mississippi State University; Estados Unidos
Fil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
MINERALOCORTICOID RECEPTOR
HIPPOCAMPUS
HYPOTHALAMUS
HYPERTENSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272146
Acceder
id CONICETDig_c9831cf8a0753959eae5ebe78030f8d2
oai_identifier_str oai:ri.conicet.gov.ar:11336/272146
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive RatsPietranera, LucianaBrocca, María ElviraCymeryng, Cora BetrizGomez Sanchez, E.Gomez Sanchez, C. E.Roig, PaulinaLima, Analia Ethelde Nicola, Alejandro FedericoMINERALOCORTICOID RECEPTORHIPPOCAMPUSHYPOTHALAMUSHYPERTENSIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The mineralocorticoid receptor (MR) has been considered both neuroprotective as well as damaging for central nervous system function. MR may be also involved in central regulation of blood pressure. In the present study we compared the expression of MR and the glucocorticoid receptor (GR) in the hippocampus and hypothalamus of 16 weeks old spontaneously hypertensive rats (SHR) and the normotensive control WKY rats. In hippocampus, MR expression was studied by in situ hybridization (ISH), qPCR, and immunohistochemistry, whereas GR expression was analyzed by the last two procedures. Hypertensive animals showed increased expression of MR mRNA in whole hippocampus according to qPCR data and also in CA3 by ISH. Immunocytochemical staining for MR of dorsal hippocampus, however, did not show differences between SHR and WKY. SHR showed elevated hypothalamic MR mRNA by qPCR, and increased number of MR immunopositive cells in the magnocellular paraventricular region, compared to WKY. In contrast, expression levels of GR mRNA or protein in hippocampus and hypothalamus of SHR were similar to those of WKY rats. Furthermore, we further investigated the role of MR in the hypertensive rats by intracerebroventricular injection of the MR antagonist RU-2831 This compound produced a significant drop in blood pressure of SHR. In conclusion, MR expression is increased in the hippocampus and hypothalamus of SHR. We suggest that pathological MR overdrive may take responsibility for up regulation of blood pressure and the encephalopathy of hypertension.Fil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Brocca, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cymeryng, Cora Betriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Gomez Sanchez, E.. Mississippi State University; Estados UnidosFil: Gomez Sanchez, C. E.. Mississippi State University; Estados UnidosFil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaWiley Blackwell Publishing, Inc2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272146Pietranera, Luciana; Brocca, María Elvira; Cymeryng, Cora Betriz; Gomez Sanchez, E.; Gomez Sanchez, C. E.; et al.; Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats; Wiley Blackwell Publishing, Inc; Journal of Neuroendocrinology; 24; 9; 8-2012; 1249-12580953-8194CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2826.2012.02332.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2826.2012.02332.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:47Zoai:ri.conicet.gov.ar:11336/272146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:47.484CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
title Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
spellingShingle Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
Pietranera, Luciana
MINERALOCORTICOID RECEPTOR
HIPPOCAMPUS
HYPOTHALAMUS
HYPERTENSION
title_short Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
title_full Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
title_fullStr Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
title_full_unstemmed Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
title_sort Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats
dc.creator.none.fl_str_mv Pietranera, Luciana
Brocca, María Elvira
Cymeryng, Cora Betriz
Gomez Sanchez, E.
Gomez Sanchez, C. E.
Roig, Paulina
Lima, Analia Ethel
de Nicola, Alejandro Federico
author Pietranera, Luciana
author_facet Pietranera, Luciana
Brocca, María Elvira
Cymeryng, Cora Betriz
Gomez Sanchez, E.
Gomez Sanchez, C. E.
Roig, Paulina
Lima, Analia Ethel
de Nicola, Alejandro Federico
author_role author
author2 Brocca, María Elvira
Cymeryng, Cora Betriz
Gomez Sanchez, E.
Gomez Sanchez, C. E.
Roig, Paulina
Lima, Analia Ethel
de Nicola, Alejandro Federico
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MINERALOCORTICOID RECEPTOR
HIPPOCAMPUS
HYPOTHALAMUS
HYPERTENSION
topic MINERALOCORTICOID RECEPTOR
HIPPOCAMPUS
HYPOTHALAMUS
HYPERTENSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The mineralocorticoid receptor (MR) has been considered both neuroprotective as well as damaging for central nervous system function. MR may be also involved in central regulation of blood pressure. In the present study we compared the expression of MR and the glucocorticoid receptor (GR) in the hippocampus and hypothalamus of 16 weeks old spontaneously hypertensive rats (SHR) and the normotensive control WKY rats. In hippocampus, MR expression was studied by in situ hybridization (ISH), qPCR, and immunohistochemistry, whereas GR expression was analyzed by the last two procedures. Hypertensive animals showed increased expression of MR mRNA in whole hippocampus according to qPCR data and also in CA3 by ISH. Immunocytochemical staining for MR of dorsal hippocampus, however, did not show differences between SHR and WKY. SHR showed elevated hypothalamic MR mRNA by qPCR, and increased number of MR immunopositive cells in the magnocellular paraventricular region, compared to WKY. In contrast, expression levels of GR mRNA or protein in hippocampus and hypothalamus of SHR were similar to those of WKY rats. Furthermore, we further investigated the role of MR in the hypertensive rats by intracerebroventricular injection of the MR antagonist RU-2831 This compound produced a significant drop in blood pressure of SHR. In conclusion, MR expression is increased in the hippocampus and hypothalamus of SHR. We suggest that pathological MR overdrive may take responsibility for up regulation of blood pressure and the encephalopathy of hypertension.
Fil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Brocca, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cymeryng, Cora Betriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Gomez Sanchez, E.. Mississippi State University; Estados Unidos
Fil: Gomez Sanchez, C. E.. Mississippi State University; Estados Unidos
Fil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description The mineralocorticoid receptor (MR) has been considered both neuroprotective as well as damaging for central nervous system function. MR may be also involved in central regulation of blood pressure. In the present study we compared the expression of MR and the glucocorticoid receptor (GR) in the hippocampus and hypothalamus of 16 weeks old spontaneously hypertensive rats (SHR) and the normotensive control WKY rats. In hippocampus, MR expression was studied by in situ hybridization (ISH), qPCR, and immunohistochemistry, whereas GR expression was analyzed by the last two procedures. Hypertensive animals showed increased expression of MR mRNA in whole hippocampus according to qPCR data and also in CA3 by ISH. Immunocytochemical staining for MR of dorsal hippocampus, however, did not show differences between SHR and WKY. SHR showed elevated hypothalamic MR mRNA by qPCR, and increased number of MR immunopositive cells in the magnocellular paraventricular region, compared to WKY. In contrast, expression levels of GR mRNA or protein in hippocampus and hypothalamus of SHR were similar to those of WKY rats. Furthermore, we further investigated the role of MR in the hypertensive rats by intracerebroventricular injection of the MR antagonist RU-2831 This compound produced a significant drop in blood pressure of SHR. In conclusion, MR expression is increased in the hippocampus and hypothalamus of SHR. We suggest that pathological MR overdrive may take responsibility for up regulation of blood pressure and the encephalopathy of hypertension.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272146
Pietranera, Luciana; Brocca, María Elvira; Cymeryng, Cora Betriz; Gomez Sanchez, E.; Gomez Sanchez, C. E.; et al.; Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats; Wiley Blackwell Publishing, Inc; Journal of Neuroendocrinology; 24; 9; 8-2012; 1249-1258
0953-8194
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272146
identifier_str_mv Pietranera, Luciana; Brocca, María Elvira; Cymeryng, Cora Betriz; Gomez Sanchez, E.; Gomez Sanchez, C. E.; et al.; Increased Expression of the Mineralocorticoid Receptor in the Brain of Spontaneously Hypertensive Rats; Wiley Blackwell Publishing, Inc; Journal of Neuroendocrinology; 24; 9; 8-2012; 1249-1258
0953-8194
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2826.2012.02332.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2826.2012.02332.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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