Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica

Autores
Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; Barry, Kerrie; Grigoriev, Igor V.; Libkind Frati, Diego; Connell, Laurie B.; Moliné, Martín
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies.
Fil: Nizovoy, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Haridas, Sajeet. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Sun, Hui. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Daum, Chris. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Barry, Kerrie. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Grigoriev, Igor V.. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Connell, Laurie B.. University of Maine; Estados Unidos
Fil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Materia
ANTARCTICA
COLD ADAPTATION
EXTREMOPHILE
GENOMICS
NAGANISHIA
YEAST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131977
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131977
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from AntarcticaNizovoy, PaulaBellora, NicolásHaridas, SajeetSun, HuiDaum, ChrisBarry, KerrieGrigoriev, Igor V.Libkind Frati, DiegoConnell, Laurie B.Moliné, MartínANTARCTICACOLD ADAPTATIONEXTREMOPHILEGENOMICSNAGANISHIAYEASThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies.Fil: Nizovoy, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Haridas, Sajeet. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Sun, Hui. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Daum, Chris. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Barry, Kerrie. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Grigoriev, Igor V.. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Connell, Laurie B.. University of Maine; Estados UnidosFil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaWiley Blackwell Publishing, Inc2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131977Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; et al.; Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica; Wiley Blackwell Publishing, Inc; FEMS yeast research; 21; 1; 1-2021; 1-421567-1364CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/advance-article/doi/10.1093/femsyr/foaa056/6000217info:eu-repo/semantics/altIdentifier/doi/10.1093/femsyr/foaa056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:12Zoai:ri.conicet.gov.ar:11336/131977instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:13.11CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
title Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
spellingShingle Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
Nizovoy, Paula
ANTARCTICA
COLD ADAPTATION
EXTREMOPHILE
GENOMICS
NAGANISHIA
YEAST
title_short Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
title_full Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
title_fullStr Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
title_full_unstemmed Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
title_sort Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
dc.creator.none.fl_str_mv Nizovoy, Paula
Bellora, Nicolás
Haridas, Sajeet
Sun, Hui
Daum, Chris
Barry, Kerrie
Grigoriev, Igor V.
Libkind Frati, Diego
Connell, Laurie B.
Moliné, Martín
author Nizovoy, Paula
author_facet Nizovoy, Paula
Bellora, Nicolás
Haridas, Sajeet
Sun, Hui
Daum, Chris
Barry, Kerrie
Grigoriev, Igor V.
Libkind Frati, Diego
Connell, Laurie B.
Moliné, Martín
author_role author
author2 Bellora, Nicolás
Haridas, Sajeet
Sun, Hui
Daum, Chris
Barry, Kerrie
Grigoriev, Igor V.
Libkind Frati, Diego
Connell, Laurie B.
Moliné, Martín
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTARCTICA
COLD ADAPTATION
EXTREMOPHILE
GENOMICS
NAGANISHIA
YEAST
topic ANTARCTICA
COLD ADAPTATION
EXTREMOPHILE
GENOMICS
NAGANISHIA
YEAST
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies.
Fil: Nizovoy, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Haridas, Sajeet. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Sun, Hui. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Daum, Chris. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Barry, Kerrie. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Grigoriev, Igor V.. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Connell, Laurie B.. University of Maine; Estados Unidos
Fil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
description Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131977
Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; et al.; Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica; Wiley Blackwell Publishing, Inc; FEMS yeast research; 21; 1; 1-2021; 1-42
1567-1364
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131977
identifier_str_mv Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; et al.; Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica; Wiley Blackwell Publishing, Inc; FEMS yeast research; 21; 1; 1-2021; 1-42
1567-1364
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/advance-article/doi/10.1093/femsyr/foaa056/6000217
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsyr/foaa056
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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