Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica
- Autores
- Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; Barry, Kerrie; Grigoriev, Igor V.; Libkind Frati, Diego; Connell, Laurie B.; Moliné, Martín
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies.
Fil: Nizovoy, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Haridas, Sajeet. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Sun, Hui. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Daum, Chris. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Barry, Kerrie. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Grigoriev, Igor V.. United States Department of Energy. Joint Genome Institute; Estados Unidos
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Connell, Laurie B.. University of Maine; Estados Unidos
Fil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina - Materia
-
ANTARCTICA
COLD ADAPTATION
EXTREMOPHILE
GENOMICS
NAGANISHIA
YEAST - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/131977
Ver los metadatos del registro completo
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Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from AntarcticaNizovoy, PaulaBellora, NicolásHaridas, SajeetSun, HuiDaum, ChrisBarry, KerrieGrigoriev, Igor V.Libkind Frati, DiegoConnell, Laurie B.Moliné, MartínANTARCTICACOLD ADAPTATIONEXTREMOPHILEGENOMICSNAGANISHIAYEASThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies.Fil: Nizovoy, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Haridas, Sajeet. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Sun, Hui. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Daum, Chris. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Barry, Kerrie. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Grigoriev, Igor V.. United States Department of Energy. Joint Genome Institute; Estados UnidosFil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Connell, Laurie B.. University of Maine; Estados UnidosFil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaWiley Blackwell Publishing, Inc2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131977Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; et al.; Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica; Wiley Blackwell Publishing, Inc; FEMS yeast research; 21; 1; 1-2021; 1-421567-1364CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/advance-article/doi/10.1093/femsyr/foaa056/6000217info:eu-repo/semantics/altIdentifier/doi/10.1093/femsyr/foaa056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:54Zoai:ri.conicet.gov.ar:11336/131977instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:54.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
title |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
spellingShingle |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica Nizovoy, Paula ANTARCTICA COLD ADAPTATION EXTREMOPHILE GENOMICS NAGANISHIA YEAST |
title_short |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
title_full |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
title_fullStr |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
title_full_unstemmed |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
title_sort |
Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica |
dc.creator.none.fl_str_mv |
Nizovoy, Paula Bellora, Nicolás Haridas, Sajeet Sun, Hui Daum, Chris Barry, Kerrie Grigoriev, Igor V. Libkind Frati, Diego Connell, Laurie B. Moliné, Martín |
author |
Nizovoy, Paula |
author_facet |
Nizovoy, Paula Bellora, Nicolás Haridas, Sajeet Sun, Hui Daum, Chris Barry, Kerrie Grigoriev, Igor V. Libkind Frati, Diego Connell, Laurie B. Moliné, Martín |
author_role |
author |
author2 |
Bellora, Nicolás Haridas, Sajeet Sun, Hui Daum, Chris Barry, Kerrie Grigoriev, Igor V. Libkind Frati, Diego Connell, Laurie B. Moliné, Martín |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
ANTARCTICA COLD ADAPTATION EXTREMOPHILE GENOMICS NAGANISHIA YEAST |
topic |
ANTARCTICA COLD ADAPTATION EXTREMOPHILE GENOMICS NAGANISHIA YEAST |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies. Fil: Nizovoy, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina Fil: Haridas, Sajeet. United States Department of Energy. Joint Genome Institute; Estados Unidos Fil: Sun, Hui. United States Department of Energy. Joint Genome Institute; Estados Unidos Fil: Daum, Chris. United States Department of Energy. Joint Genome Institute; Estados Unidos Fil: Barry, Kerrie. United States Department of Energy. Joint Genome Institute; Estados Unidos Fil: Grigoriev, Igor V.. United States Department of Energy. Joint Genome Institute; Estados Unidos Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina Fil: Connell, Laurie B.. University of Maine; Estados Unidos Fil: Moliné, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina |
description |
Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of N. vishniacii. Using comparative genomics, we unraveled unique characteristics of cold associated adaptations. From a total of 6183 predicted coding sequences, we identified 336 genes absent in sister species, encoding solute transfers and chaperones, among others. Among genes shared by N. vishniacii and its closest related species we found orthologs encompassing possible evidence of positive selection (dN/dS > 1). Genes associated with photoprotection were found in agreement with high solar irradiation exposure. Also genes coding for desaturases and genomic features associated with cold tolerance (i.e. trehalose synthesis and lipid metabolism) were explored. Finally, biases in amino acid usage (namely enrichment of glutamine and a trend reduction of proline) were observed, possibly conferring increased protein flexibility. To the best of our knowledge, such a combination of mechanisms for cold tolerance has not been previously reported in fungi, making N. vishniacii a unique model for the study of the genetic basis and evolution of cold adaptation strategies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/131977 Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; et al.; Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica; Wiley Blackwell Publishing, Inc; FEMS yeast research; 21; 1; 1-2021; 1-42 1567-1364 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/131977 |
identifier_str_mv |
Nizovoy, Paula; Bellora, Nicolás; Haridas, Sajeet; Sun, Hui; Daum, Chris; et al.; Unique genomic traits for cold adaptation in Naganishia vishniacii, a polyextremophile yeast isolated from Antarctica; Wiley Blackwell Publishing, Inc; FEMS yeast research; 21; 1; 1-2021; 1-42 1567-1364 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/advance-article/doi/10.1093/femsyr/foaa056/6000217 info:eu-repo/semantics/altIdentifier/doi/10.1093/femsyr/foaa056 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613230008205312 |
score |
13.070432 |