Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides

Autores
Filippi, Iohanna; Fernández, Pilar; Grimalt, Joan O.; Butinof, Mariana; Amé, María Valeria; Muñoz, Sonia Edith
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human biomonitoring of the exposure to pesticides is usually performed in biological matrices such as urine and plasma. However, the possibility of using less invasive matrices allows the screening of large number of subjects. The objective of this study was to evaluate the body burden of the exposure to the most widely used herbicide, Glyphosate (GLY), and its main metabolite, the aminomethylphosphonic acid (AMPA), in different populations from the province of Córdoba (Argentina), and to propose the saliva as a matrix for possible implementation in biomonitoring of the exposure to pesticides. Glyphosate and AMPA concentrations were evaluated in urine, plasma, and saliva of subjects occupationally and environmentally exposed to pesticides from one of the most important agricultural areas of Argentina. Gas chromatography coupled to tandem mass spectrometry (MS/MS) was used for the identification and quantification of the analytes. Both GLY and AMPA were quantified in all matrices with higher detection frequency (DF) in the occupationally exposed group than in non-occupationally exposed individuals. Among evaluated matrices, the highest DF and concentration levels of GLY were found in saliva. Moreover, the only statistical difference between groups of subjects were found for GLY and AMPA concentrations in saliva, indicating the possible use of this non-invasive human matrix to evaluate different levels and scenarios of exposure. No significant correlation was found between GLY and AMPA levels in saliva and the traditional matrices (urine and blood) used to measure exposure to pesticides. This is the first report of the presence and concentrations of GLY and AMPA in human saliva samples. Results of the present study are relevant for future biomonitoring of the exposure to GLY, but also to pesticides in general. Saliva deserved further investigation as an alternative, easy, and economical matrix involving less invasive methods for biomonitoring and screenings of large populations.
Fil: Filippi, Iohanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Fernández, Pilar. Consejo Superior de Investigaciones Científicas; España
Fil: Grimalt, Joan O.. Consejo Superior de Investigaciones Científicas; España
Fil: Butinof, Mariana. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Muñoz, Sonia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Materia
PESTICIDES, OCCUPATIONAL EXPOSURE
PLASMA
SALIVA
URINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260859
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/260859
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticidesFilippi, IohannaFernández, PilarGrimalt, Joan O.Butinof, MarianaAmé, María ValeriaMuñoz, Sonia EdithPESTICIDES, OCCUPATIONAL EXPOSUREPLASMASALIVAURINEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Human biomonitoring of the exposure to pesticides is usually performed in biological matrices such as urine and plasma. However, the possibility of using less invasive matrices allows the screening of large number of subjects. The objective of this study was to evaluate the body burden of the exposure to the most widely used herbicide, Glyphosate (GLY), and its main metabolite, the aminomethylphosphonic acid (AMPA), in different populations from the province of Córdoba (Argentina), and to propose the saliva as a matrix for possible implementation in biomonitoring of the exposure to pesticides. Glyphosate and AMPA concentrations were evaluated in urine, plasma, and saliva of subjects occupationally and environmentally exposed to pesticides from one of the most important agricultural areas of Argentina. Gas chromatography coupled to tandem mass spectrometry (MS/MS) was used for the identification and quantification of the analytes. Both GLY and AMPA were quantified in all matrices with higher detection frequency (DF) in the occupationally exposed group than in non-occupationally exposed individuals. Among evaluated matrices, the highest DF and concentration levels of GLY were found in saliva. Moreover, the only statistical difference between groups of subjects were found for GLY and AMPA concentrations in saliva, indicating the possible use of this non-invasive human matrix to evaluate different levels and scenarios of exposure. No significant correlation was found between GLY and AMPA levels in saliva and the traditional matrices (urine and blood) used to measure exposure to pesticides. This is the first report of the presence and concentrations of GLY and AMPA in human saliva samples. Results of the present study are relevant for future biomonitoring of the exposure to GLY, but also to pesticides in general. Saliva deserved further investigation as an alternative, easy, and economical matrix involving less invasive methods for biomonitoring and screenings of large populations.Fil: Filippi, Iohanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Fernández, Pilar. Consejo Superior de Investigaciones Científicas; EspañaFil: Grimalt, Joan O.. Consejo Superior de Investigaciones Científicas; EspañaFil: Butinof, Mariana. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Muñoz, Sonia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaElsevier2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260859Filippi, Iohanna; Fernández, Pilar; Grimalt, Joan O.; Butinof, Mariana; Amé, María Valeria; et al.; Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides; Elsevier; Environmental Advances; 15; 4-2024; 1-82666-7657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2666765723001321info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envadv.2023.100474info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:15Zoai:ri.conicet.gov.ar:11336/260859instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:15.276CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
title Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
spellingShingle Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
Filippi, Iohanna
PESTICIDES, OCCUPATIONAL EXPOSURE
PLASMA
SALIVA
URINE
title_short Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
title_full Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
title_fullStr Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
title_full_unstemmed Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
title_sort Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides
dc.creator.none.fl_str_mv Filippi, Iohanna
Fernández, Pilar
Grimalt, Joan O.
Butinof, Mariana
Amé, María Valeria
Muñoz, Sonia Edith
author Filippi, Iohanna
author_facet Filippi, Iohanna
Fernández, Pilar
Grimalt, Joan O.
Butinof, Mariana
Amé, María Valeria
Muñoz, Sonia Edith
author_role author
author2 Fernández, Pilar
Grimalt, Joan O.
Butinof, Mariana
Amé, María Valeria
Muñoz, Sonia Edith
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PESTICIDES, OCCUPATIONAL EXPOSURE
PLASMA
SALIVA
URINE
topic PESTICIDES, OCCUPATIONAL EXPOSURE
PLASMA
SALIVA
URINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Human biomonitoring of the exposure to pesticides is usually performed in biological matrices such as urine and plasma. However, the possibility of using less invasive matrices allows the screening of large number of subjects. The objective of this study was to evaluate the body burden of the exposure to the most widely used herbicide, Glyphosate (GLY), and its main metabolite, the aminomethylphosphonic acid (AMPA), in different populations from the province of Córdoba (Argentina), and to propose the saliva as a matrix for possible implementation in biomonitoring of the exposure to pesticides. Glyphosate and AMPA concentrations were evaluated in urine, plasma, and saliva of subjects occupationally and environmentally exposed to pesticides from one of the most important agricultural areas of Argentina. Gas chromatography coupled to tandem mass spectrometry (MS/MS) was used for the identification and quantification of the analytes. Both GLY and AMPA were quantified in all matrices with higher detection frequency (DF) in the occupationally exposed group than in non-occupationally exposed individuals. Among evaluated matrices, the highest DF and concentration levels of GLY were found in saliva. Moreover, the only statistical difference between groups of subjects were found for GLY and AMPA concentrations in saliva, indicating the possible use of this non-invasive human matrix to evaluate different levels and scenarios of exposure. No significant correlation was found between GLY and AMPA levels in saliva and the traditional matrices (urine and blood) used to measure exposure to pesticides. This is the first report of the presence and concentrations of GLY and AMPA in human saliva samples. Results of the present study are relevant for future biomonitoring of the exposure to GLY, but also to pesticides in general. Saliva deserved further investigation as an alternative, easy, and economical matrix involving less invasive methods for biomonitoring and screenings of large populations.
Fil: Filippi, Iohanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Fernández, Pilar. Consejo Superior de Investigaciones Científicas; España
Fil: Grimalt, Joan O.. Consejo Superior de Investigaciones Científicas; España
Fil: Butinof, Mariana. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Muñoz, Sonia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
description Human biomonitoring of the exposure to pesticides is usually performed in biological matrices such as urine and plasma. However, the possibility of using less invasive matrices allows the screening of large number of subjects. The objective of this study was to evaluate the body burden of the exposure to the most widely used herbicide, Glyphosate (GLY), and its main metabolite, the aminomethylphosphonic acid (AMPA), in different populations from the province of Córdoba (Argentina), and to propose the saliva as a matrix for possible implementation in biomonitoring of the exposure to pesticides. Glyphosate and AMPA concentrations were evaluated in urine, plasma, and saliva of subjects occupationally and environmentally exposed to pesticides from one of the most important agricultural areas of Argentina. Gas chromatography coupled to tandem mass spectrometry (MS/MS) was used for the identification and quantification of the analytes. Both GLY and AMPA were quantified in all matrices with higher detection frequency (DF) in the occupationally exposed group than in non-occupationally exposed individuals. Among evaluated matrices, the highest DF and concentration levels of GLY were found in saliva. Moreover, the only statistical difference between groups of subjects were found for GLY and AMPA concentrations in saliva, indicating the possible use of this non-invasive human matrix to evaluate different levels and scenarios of exposure. No significant correlation was found between GLY and AMPA levels in saliva and the traditional matrices (urine and blood) used to measure exposure to pesticides. This is the first report of the presence and concentrations of GLY and AMPA in human saliva samples. Results of the present study are relevant for future biomonitoring of the exposure to GLY, but also to pesticides in general. Saliva deserved further investigation as an alternative, easy, and economical matrix involving less invasive methods for biomonitoring and screenings of large populations.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260859
Filippi, Iohanna; Fernández, Pilar; Grimalt, Joan O.; Butinof, Mariana; Amé, María Valeria; et al.; Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides; Elsevier; Environmental Advances; 15; 4-2024; 1-8
2666-7657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260859
identifier_str_mv Filippi, Iohanna; Fernández, Pilar; Grimalt, Joan O.; Butinof, Mariana; Amé, María Valeria; et al.; Glyphosate and AMPA in saliva and other traditional human matrices. New findings for less invasive biomonitoring to the exposure to pesticides; Elsevier; Environmental Advances; 15; 4-2024; 1-8
2666-7657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2666765723001321
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envadv.2023.100474
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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