Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017

Autores
García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; Muñoz Chimeno, Milagros; Fernández Manzano, Alvaro; Escobar, Franco Matias; Martínez, Irene; Bárcena, Carmen; González, Sergio; Avellón, Ana; Eiros, Jose M.; Fongaro, Gislaine; Domínguez, Lucas; Goyache, Joaquín; Rodríguez Lázaro, David
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
Fil: García, Nerea. Universidad Complutense de Madrid; España
Fil: Hernández, Marta. Universidad de Burgos; España
Fil: Gutierrez Boada, Maialen. Universidad de Burgos; España
Fil: Valero, Antonio. Universidad de Córdoba; España
Fil: Navarro, Alejandro. Universidad Complutense de Madrid; España
Fil: Muñoz Chimeno, Milagros. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Fernández Manzano, Alvaro. Universidad Complutense de Madrid; España
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Martínez, Irene. Universidad Complutense de Madrid; España
Fil: Bárcena, Carmen. Universidad Complutense de Madrid; España
Fil: González, Sergio. Universidad Complutense de Madrid; España
Fil: Avellón, Ana. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Eiros, Jose M.. Hospital Universitario Rio Hortega; España
Fil: Fongaro, Gislaine. Universidade Federal de Santa Catarina; Brasil
Fil: Domínguez, Lucas. Universidad Complutense de Madrid; España
Fil: Goyache, Joaquín. Universidad Complutense de Madrid; España
Fil: Rodríguez Lázaro, David. Universidad de Burgos; España
Materia
FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132427
Acceder
id CONICETDig_c91b56ab6efb295ee90516593ea1a90c
oai_identifier_str oai:ri.conicet.gov.ar:11336/132427
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017García, NereaHernández, MartaGutierrez Boada, MaialenValero, AntonioNavarro, AlejandroMuñoz Chimeno, MilagrosFernández Manzano, AlvaroEscobar, Franco MatiasMartínez, IreneBárcena, CarmenGonzález, SergioAvellón, AnaEiros, Jose M.Fongaro, GislaineDomínguez, LucasGoyache, JoaquínRodríguez Lázaro, DavidFOOD SAFETYHEPATITIS E VIRUSPIG ORGANSPIGSPORK CUTTINGSPREVALENCESEROPREVALENCESLAUGHTERHOUSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.Fil: García, Nerea. Universidad Complutense de Madrid; EspañaFil: Hernández, Marta. Universidad de Burgos; EspañaFil: Gutierrez Boada, Maialen. Universidad de Burgos; EspañaFil: Valero, Antonio. Universidad de Córdoba; EspañaFil: Navarro, Alejandro. Universidad Complutense de Madrid; EspañaFil: Muñoz Chimeno, Milagros. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Fernández Manzano, Alvaro. Universidad Complutense de Madrid; EspañaFil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Martínez, Irene. Universidad Complutense de Madrid; EspañaFil: Bárcena, Carmen. Universidad Complutense de Madrid; EspañaFil: González, Sergio. Universidad Complutense de Madrid; EspañaFil: Avellón, Ana. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Eiros, Jose M.. Hospital Universitario Rio Hortega; EspañaFil: Fongaro, Gislaine. Universidade Federal de Santa Catarina; BrasilFil: Domínguez, Lucas. Universidad Complutense de Madrid; EspañaFil: Goyache, Joaquín. Universidad Complutense de Madrid; EspañaFil: Rodríguez Lázaro, David. Universidad de Burgos; EspañaFrontiers Media S.A.2020-01-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132427García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; et al.; Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017; Frontiers Media S.A.; Frontiers in Microbiology; 10; 2990; 28-1-2020; 1-101664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.02990/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02990info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:40:35Zoai:ri.conicet.gov.ar:11336/132427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:40:35.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
spellingShingle Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
García, Nerea
FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
title_short Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_full Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_fullStr Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_full_unstemmed Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_sort Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
dc.creator.none.fl_str_mv García, Nerea
Hernández, Marta
Gutierrez Boada, Maialen
Valero, Antonio
Navarro, Alejandro
Muñoz Chimeno, Milagros
Fernández Manzano, Alvaro
Escobar, Franco Matias
Martínez, Irene
Bárcena, Carmen
González, Sergio
Avellón, Ana
Eiros, Jose M.
Fongaro, Gislaine
Domínguez, Lucas
Goyache, Joaquín
Rodríguez Lázaro, David
author García, Nerea
author_facet García, Nerea
Hernández, Marta
Gutierrez Boada, Maialen
Valero, Antonio
Navarro, Alejandro
Muñoz Chimeno, Milagros
Fernández Manzano, Alvaro
Escobar, Franco Matias
Martínez, Irene
Bárcena, Carmen
González, Sergio
Avellón, Ana
Eiros, Jose M.
Fongaro, Gislaine
Domínguez, Lucas
Goyache, Joaquín
Rodríguez Lázaro, David
author_role author
author2 Hernández, Marta
Gutierrez Boada, Maialen
Valero, Antonio
Navarro, Alejandro
Muñoz Chimeno, Milagros
Fernández Manzano, Alvaro
Escobar, Franco Matias
Martínez, Irene
Bárcena, Carmen
González, Sergio
Avellón, Ana
Eiros, Jose M.
Fongaro, Gislaine
Domínguez, Lucas
Goyache, Joaquín
Rodríguez Lázaro, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
topic FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
Fil: García, Nerea. Universidad Complutense de Madrid; España
Fil: Hernández, Marta. Universidad de Burgos; España
Fil: Gutierrez Boada, Maialen. Universidad de Burgos; España
Fil: Valero, Antonio. Universidad de Córdoba; España
Fil: Navarro, Alejandro. Universidad Complutense de Madrid; España
Fil: Muñoz Chimeno, Milagros. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Fernández Manzano, Alvaro. Universidad Complutense de Madrid; España
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Martínez, Irene. Universidad Complutense de Madrid; España
Fil: Bárcena, Carmen. Universidad Complutense de Madrid; España
Fil: González, Sergio. Universidad Complutense de Madrid; España
Fil: Avellón, Ana. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Eiros, Jose M.. Hospital Universitario Rio Hortega; España
Fil: Fongaro, Gislaine. Universidade Federal de Santa Catarina; Brasil
Fil: Domínguez, Lucas. Universidad Complutense de Madrid; España
Fil: Goyache, Joaquín. Universidad Complutense de Madrid; España
Fil: Rodríguez Lázaro, David. Universidad de Burgos; España
description Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132427
García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; et al.; Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017; Frontiers Media S.A.; Frontiers in Microbiology; 10; 2990; 28-1-2020; 1-10
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132427
identifier_str_mv García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; et al.; Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017; Frontiers Media S.A.; Frontiers in Microbiology; 10; 2990; 28-1-2020; 1-10
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.02990/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02990
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.106097

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