First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition

Autores
Sun, Jin; Zhang, Huoming; Wang, Hao; Heras, Horacio; Dreon, Marcos Sebastian; Ituarte, Santiago; Ravasi, Timothy; Qian, Pei-Yuan; Qiu, Jian-Wen
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pomacea canaliculata is a freshwater snail that deposits eggs on solid substrates above the water surface. Previous studies have emphasized the nutritional and protective functions of the three most abundant perivitelline fluid (PVF) protein complexes (ovorubin, PV2, and PV3) during its embryonic development, but little is known about the structure and function of other less abundant proteins. Using 2-DE, SDS-PAGE, MALDI TOF/TOF, and LC-MS/MS, we identified 59 proteins from the PVF of P. canaliculata, among which 19 are novel. KEGG analysis showed that the functions of the majority of these proteins are "unknown" (n = 34), "environmental information processing" (10), 9 of which are related to innate immunity, and "metabolism" (7). Suppressive subtractive hybridization revealed 21 PVF genes to be specific to the albumen gland, indicating this organ is the origin of many of the PVF proteins. Further, the 3 ovorubin subunits were identified with 30.2-35.0% identity among them, indicating their common origin but ancient duplications. Characterization of the PVF proteome has opened the gate for further studies aiming to understand the evolution of the novel proteins and their contribution to the switch to aerial oviposition.
Fil: Sun, Jin. Hong Kong Baptist University; Hong Kong
Fil: Zhang, Huoming. Hong Kong Baptist University; Hong Kong
Fil: Wang, Hao. Hong Kong University Of Science And Technology; Hong Kong
Fil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Ituarte, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Ravasi, Timothy. King Abdullah University Of Science And Technology; Arabia Saudita
Fil: Qian, Pei-Yuan. Hong Kong University Of Science And Technology; Hong Kong
Fil: Qiu, Jian-Wen. Hong Kong Baptist University; Hong Kong
Materia
APPLE SNAIL
NOVEL PROTEIN
PERIVITELLINE FLUID
POMACEA CANALICULATA
PROTEOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94414
Acceder
id CONICETDig_c855d137afa0c4acc108c71bc9ae6f51
oai_identifier_str oai:ri.conicet.gov.ar:11336/94414
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial ovipositionSun, JinZhang, HuomingWang, HaoHeras, HoracioDreon, Marcos SebastianItuarte, SantiagoRavasi, TimothyQian, Pei-YuanQiu, Jian-WenAPPLE SNAILNOVEL PROTEINPERIVITELLINE FLUIDPOMACEA CANALICULATAPROTEOMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pomacea canaliculata is a freshwater snail that deposits eggs on solid substrates above the water surface. Previous studies have emphasized the nutritional and protective functions of the three most abundant perivitelline fluid (PVF) protein complexes (ovorubin, PV2, and PV3) during its embryonic development, but little is known about the structure and function of other less abundant proteins. Using 2-DE, SDS-PAGE, MALDI TOF/TOF, and LC-MS/MS, we identified 59 proteins from the PVF of P. canaliculata, among which 19 are novel. KEGG analysis showed that the functions of the majority of these proteins are "unknown" (n = 34), "environmental information processing" (10), 9 of which are related to innate immunity, and "metabolism" (7). Suppressive subtractive hybridization revealed 21 PVF genes to be specific to the albumen gland, indicating this organ is the origin of many of the PVF proteins. Further, the 3 ovorubin subunits were identified with 30.2-35.0% identity among them, indicating their common origin but ancient duplications. Characterization of the PVF proteome has opened the gate for further studies aiming to understand the evolution of the novel proteins and their contribution to the switch to aerial oviposition.Fil: Sun, Jin. Hong Kong Baptist University; Hong KongFil: Zhang, Huoming. Hong Kong Baptist University; Hong KongFil: Wang, Hao. Hong Kong University Of Science And Technology; Hong KongFil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Ituarte, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Ravasi, Timothy. King Abdullah University Of Science And Technology; Arabia SauditaFil: Qian, Pei-Yuan. Hong Kong University Of Science And Technology; Hong KongFil: Qiu, Jian-Wen. Hong Kong Baptist University; Hong KongAmerican Chemical Society2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94414Sun, Jin; Zhang, Huoming; Wang, Hao; Heras, Horacio; Dreon, Marcos Sebastian; et al.; First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition; American Chemical Society; Journal of Proteome Research; 11; 8; 8-2012; 4240-42481535-3893CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/pr3003613info:eu-repo/semantics/altIdentifier/doi/10.1021/pr3003613info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:25Zoai:ri.conicet.gov.ar:11336/94414instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:25.478CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
title First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
spellingShingle First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
Sun, Jin
APPLE SNAIL
NOVEL PROTEIN
PERIVITELLINE FLUID
POMACEA CANALICULATA
PROTEOMICS
title_short First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
title_full First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
title_fullStr First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
title_full_unstemmed First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
title_sort First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition
dc.creator.none.fl_str_mv Sun, Jin
Zhang, Huoming
Wang, Hao
Heras, Horacio
Dreon, Marcos Sebastian
Ituarte, Santiago
Ravasi, Timothy
Qian, Pei-Yuan
Qiu, Jian-Wen
author Sun, Jin
author_facet Sun, Jin
Zhang, Huoming
Wang, Hao
Heras, Horacio
Dreon, Marcos Sebastian
Ituarte, Santiago
Ravasi, Timothy
Qian, Pei-Yuan
Qiu, Jian-Wen
author_role author
author2 Zhang, Huoming
Wang, Hao
Heras, Horacio
Dreon, Marcos Sebastian
Ituarte, Santiago
Ravasi, Timothy
Qian, Pei-Yuan
Qiu, Jian-Wen
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APPLE SNAIL
NOVEL PROTEIN
PERIVITELLINE FLUID
POMACEA CANALICULATA
PROTEOMICS
topic APPLE SNAIL
NOVEL PROTEIN
PERIVITELLINE FLUID
POMACEA CANALICULATA
PROTEOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pomacea canaliculata is a freshwater snail that deposits eggs on solid substrates above the water surface. Previous studies have emphasized the nutritional and protective functions of the three most abundant perivitelline fluid (PVF) protein complexes (ovorubin, PV2, and PV3) during its embryonic development, but little is known about the structure and function of other less abundant proteins. Using 2-DE, SDS-PAGE, MALDI TOF/TOF, and LC-MS/MS, we identified 59 proteins from the PVF of P. canaliculata, among which 19 are novel. KEGG analysis showed that the functions of the majority of these proteins are "unknown" (n = 34), "environmental information processing" (10), 9 of which are related to innate immunity, and "metabolism" (7). Suppressive subtractive hybridization revealed 21 PVF genes to be specific to the albumen gland, indicating this organ is the origin of many of the PVF proteins. Further, the 3 ovorubin subunits were identified with 30.2-35.0% identity among them, indicating their common origin but ancient duplications. Characterization of the PVF proteome has opened the gate for further studies aiming to understand the evolution of the novel proteins and their contribution to the switch to aerial oviposition.
Fil: Sun, Jin. Hong Kong Baptist University; Hong Kong
Fil: Zhang, Huoming. Hong Kong Baptist University; Hong Kong
Fil: Wang, Hao. Hong Kong University Of Science And Technology; Hong Kong
Fil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Ituarte, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Ravasi, Timothy. King Abdullah University Of Science And Technology; Arabia Saudita
Fil: Qian, Pei-Yuan. Hong Kong University Of Science And Technology; Hong Kong
Fil: Qiu, Jian-Wen. Hong Kong Baptist University; Hong Kong
description Pomacea canaliculata is a freshwater snail that deposits eggs on solid substrates above the water surface. Previous studies have emphasized the nutritional and protective functions of the three most abundant perivitelline fluid (PVF) protein complexes (ovorubin, PV2, and PV3) during its embryonic development, but little is known about the structure and function of other less abundant proteins. Using 2-DE, SDS-PAGE, MALDI TOF/TOF, and LC-MS/MS, we identified 59 proteins from the PVF of P. canaliculata, among which 19 are novel. KEGG analysis showed that the functions of the majority of these proteins are "unknown" (n = 34), "environmental information processing" (10), 9 of which are related to innate immunity, and "metabolism" (7). Suppressive subtractive hybridization revealed 21 PVF genes to be specific to the albumen gland, indicating this organ is the origin of many of the PVF proteins. Further, the 3 ovorubin subunits were identified with 30.2-35.0% identity among them, indicating their common origin but ancient duplications. Characterization of the PVF proteome has opened the gate for further studies aiming to understand the evolution of the novel proteins and their contribution to the switch to aerial oviposition.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94414
Sun, Jin; Zhang, Huoming; Wang, Hao; Heras, Horacio; Dreon, Marcos Sebastian; et al.; First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition; American Chemical Society; Journal of Proteome Research; 11; 8; 8-2012; 4240-4248
1535-3893
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94414
identifier_str_mv Sun, Jin; Zhang, Huoming; Wang, Hao; Heras, Horacio; Dreon, Marcos Sebastian; et al.; First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition; American Chemical Society; Journal of Proteome Research; 11; 8; 8-2012; 4240-4248
1535-3893
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/pr3003613
info:eu-repo/semantics/altIdentifier/doi/10.1021/pr3003613
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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