Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection

Autores
Scandiani, María M.; Luque, Alicia G.; Razori, María V.; Ciancio Casalini, Lucila; Aoki, Takayuki; O´Donnell, Kerry; Cervigni, Gerardo Domingo Lucio; Spampinato, Claudia Patricia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean germplasm exhibits various levels of resistance to Fusarium tucumaniae, the main causal agent of sudden death syndrome (SDS) of soybean in Argentina. In this study, two soybean genotypes, one susceptible (NA 4613) and one partially resistant (DM 4670) to SDS infection, were inoculated with F. tucumaniae. Disease symptoms were scored at 7, 10, 14, and 25 days post-inoculation (dpi). The greatest difference in the area under the disease progress curve (AUDPC) values among genotypes was observed at 25 dpi. In order to detect early metabolic markers that could potentially discriminate between susceptible and resistant genotypes, gas chromatography–mass spectrometry (GC-MS) analyses of root samples were performed. These analyses show higher levels of several amino acids and the polyamine cadaverine in the inoculated than in the uninoculated susceptible cultivar at 7 dpi. Principal component analysis (PCA) revealed that the metabolic profile of roots harvested at the earliest time points from the inoculated susceptible genotype was clearly differentiated from the rest of the samples. Furthermore, variables associated with the first principal component were mainly amino acids. Taken together, the results indicate that the pathogen induced the susceptible plant to accumulate amino acids in roots at early time points after infection, suggesting that GC-MS-based metabolomics could be used for the rapid characterization of cultivar response to SDS.
Fil: Scandiani, María M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Luque, Alicia G.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Razori, María V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Ciancio Casalini, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Aoki, Takayuki. Genetic Resources Center; Japón
Fil: O´Donnell, Kerry. United States Department Of Agriculture; Estados Unidos
Fil: Cervigni, Gerardo Domingo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Materia
Gc-Ms
Glycine Max
Metabolomics
Pathogen Infection
Plant Susceptibility
Sudden Death Syndrome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7860
Acceder
id CONICETDig_c68092309e8ca57bc97fdb6bfd11f130
oai_identifier_str oai:ri.conicet.gov.ar:11336/7860
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infectionScandiani, María M.Luque, Alicia G.Razori, María V.Ciancio Casalini, LucilaAoki, TakayukiO´Donnell, KerryCervigni, Gerardo Domingo LucioSpampinato, Claudia PatriciaGc-MsGlycine MaxMetabolomicsPathogen InfectionPlant SusceptibilitySudden Death Syndromehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soybean germplasm exhibits various levels of resistance to Fusarium tucumaniae, the main causal agent of sudden death syndrome (SDS) of soybean in Argentina. In this study, two soybean genotypes, one susceptible (NA 4613) and one partially resistant (DM 4670) to SDS infection, were inoculated with F. tucumaniae. Disease symptoms were scored at 7, 10, 14, and 25 days post-inoculation (dpi). The greatest difference in the area under the disease progress curve (AUDPC) values among genotypes was observed at 25 dpi. In order to detect early metabolic markers that could potentially discriminate between susceptible and resistant genotypes, gas chromatography–mass spectrometry (GC-MS) analyses of root samples were performed. These analyses show higher levels of several amino acids and the polyamine cadaverine in the inoculated than in the uninoculated susceptible cultivar at 7 dpi. Principal component analysis (PCA) revealed that the metabolic profile of roots harvested at the earliest time points from the inoculated susceptible genotype was clearly differentiated from the rest of the samples. Furthermore, variables associated with the first principal component were mainly amino acids. Taken together, the results indicate that the pathogen induced the susceptible plant to accumulate amino acids in roots at early time points after infection, suggesting that GC-MS-based metabolomics could be used for the rapid characterization of cultivar response to SDS.Fil: Scandiani, María M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Luque, Alicia G.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Razori, María V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Ciancio Casalini, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Aoki, Takayuki. Genetic Resources Center; JapónFil: O´Donnell, Kerry. United States Department Of Agriculture; Estados UnidosFil: Cervigni, Gerardo Domingo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaOxford University Press2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7860Scandiani, María M.; Luque, Alicia G.; Razori, María V.; Ciancio Casalini, Lucila; Aoki, Takayuki; et al.; Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection; Oxford University Press; Journal Of Experimental Botany; 66; 1; 1-2015; 391-4020022-0957enginfo:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/66/1/391.longinfo:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eru432info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:52:58Zoai:ri.conicet.gov.ar:11336/7860instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:52:58.912CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
title Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
spellingShingle Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
Scandiani, María M.
Gc-Ms
Glycine Max
Metabolomics
Pathogen Infection
Plant Susceptibility
Sudden Death Syndrome
title_short Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
title_full Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
title_fullStr Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
title_full_unstemmed Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
title_sort Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection
dc.creator.none.fl_str_mv Scandiani, María M.
Luque, Alicia G.
Razori, María V.
Ciancio Casalini, Lucila
Aoki, Takayuki
O´Donnell, Kerry
Cervigni, Gerardo Domingo Lucio
Spampinato, Claudia Patricia
author Scandiani, María M.
author_facet Scandiani, María M.
Luque, Alicia G.
Razori, María V.
Ciancio Casalini, Lucila
Aoki, Takayuki
O´Donnell, Kerry
Cervigni, Gerardo Domingo Lucio
Spampinato, Claudia Patricia
author_role author
author2 Luque, Alicia G.
Razori, María V.
Ciancio Casalini, Lucila
Aoki, Takayuki
O´Donnell, Kerry
Cervigni, Gerardo Domingo Lucio
Spampinato, Claudia Patricia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gc-Ms
Glycine Max
Metabolomics
Pathogen Infection
Plant Susceptibility
Sudden Death Syndrome
topic Gc-Ms
Glycine Max
Metabolomics
Pathogen Infection
Plant Susceptibility
Sudden Death Syndrome
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soybean germplasm exhibits various levels of resistance to Fusarium tucumaniae, the main causal agent of sudden death syndrome (SDS) of soybean in Argentina. In this study, two soybean genotypes, one susceptible (NA 4613) and one partially resistant (DM 4670) to SDS infection, were inoculated with F. tucumaniae. Disease symptoms were scored at 7, 10, 14, and 25 days post-inoculation (dpi). The greatest difference in the area under the disease progress curve (AUDPC) values among genotypes was observed at 25 dpi. In order to detect early metabolic markers that could potentially discriminate between susceptible and resistant genotypes, gas chromatography–mass spectrometry (GC-MS) analyses of root samples were performed. These analyses show higher levels of several amino acids and the polyamine cadaverine in the inoculated than in the uninoculated susceptible cultivar at 7 dpi. Principal component analysis (PCA) revealed that the metabolic profile of roots harvested at the earliest time points from the inoculated susceptible genotype was clearly differentiated from the rest of the samples. Furthermore, variables associated with the first principal component were mainly amino acids. Taken together, the results indicate that the pathogen induced the susceptible plant to accumulate amino acids in roots at early time points after infection, suggesting that GC-MS-based metabolomics could be used for the rapid characterization of cultivar response to SDS.
Fil: Scandiani, María M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Luque, Alicia G.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Razori, María V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Ciancio Casalini, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Aoki, Takayuki. Genetic Resources Center; Japón
Fil: O´Donnell, Kerry. United States Department Of Agriculture; Estados Unidos
Fil: Cervigni, Gerardo Domingo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
description Soybean germplasm exhibits various levels of resistance to Fusarium tucumaniae, the main causal agent of sudden death syndrome (SDS) of soybean in Argentina. In this study, two soybean genotypes, one susceptible (NA 4613) and one partially resistant (DM 4670) to SDS infection, were inoculated with F. tucumaniae. Disease symptoms were scored at 7, 10, 14, and 25 days post-inoculation (dpi). The greatest difference in the area under the disease progress curve (AUDPC) values among genotypes was observed at 25 dpi. In order to detect early metabolic markers that could potentially discriminate between susceptible and resistant genotypes, gas chromatography–mass spectrometry (GC-MS) analyses of root samples were performed. These analyses show higher levels of several amino acids and the polyamine cadaverine in the inoculated than in the uninoculated susceptible cultivar at 7 dpi. Principal component analysis (PCA) revealed that the metabolic profile of roots harvested at the earliest time points from the inoculated susceptible genotype was clearly differentiated from the rest of the samples. Furthermore, variables associated with the first principal component were mainly amino acids. Taken together, the results indicate that the pathogen induced the susceptible plant to accumulate amino acids in roots at early time points after infection, suggesting that GC-MS-based metabolomics could be used for the rapid characterization of cultivar response to SDS.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7860
Scandiani, María M.; Luque, Alicia G.; Razori, María V.; Ciancio Casalini, Lucila; Aoki, Takayuki; et al.; Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection; Oxford University Press; Journal Of Experimental Botany; 66; 1; 1-2015; 391-402
0022-0957
url http://hdl.handle.net/11336/7860
identifier_str_mv Scandiani, María M.; Luque, Alicia G.; Razori, María V.; Ciancio Casalini, Lucila; Aoki, Takayuki; et al.; Metabolic profiles of soybean roots during early stages of Fusarium tucumaniae infection; Oxford University Press; Journal Of Experimental Botany; 66; 1; 1-2015; 391-402
0022-0957
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/66/1/391.long
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eru432
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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