Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats

Autores
Rey Funes, Manuel; Contartese, Daniela Soledad; Peláez, Rafael; García Sanmartín, Josune; Narro Íñiguez, Judit; Soliño, Manuel; Fernández, Juan Carlos; Sarotto, Aníbal José; Ciranna, Nicolás S.; López Costa, Juan José; Dorfman, Verónica Berta; Larráyoz, Ignacio M.; Loidl, C. Fabián; Martínez, Alfredo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.
Fil: Rey Funes, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Contartese, Daniela Soledad. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Peláez, Rafael. Centro de Investigación Biomédica de La Rioja; España
Fil: García Sanmartín, Josune. Centro de Investigación Biomédica de La Rioja; España
Fil: Narro Íñiguez, Judit. Centro de Investigación Biomédica de La Rioja; España
Fil: Soliño, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Fernández, Juan Carlos. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Sarotto, Aníbal José. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Ciranna, Nicolás S.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: López Costa, Juan José. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Larráyoz, Ignacio M.. Centro de Investigación Biomédica de La Rioja; España
Fil: Loidl, C. Fabián. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Martínez, Alfredo. Centro de Investigación Biomédica de La Rioja; España
Materia
APOPTOSIS
COLD-SHOCK PROTEINS
ELECTRORETINOGRAM
HYPOTHERMIA
PERINATAL ASPHYXIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/165332
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/165332
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hypothermic shock applied after perinatal asphyxia prevents retinal damage in ratsRey Funes, ManuelContartese, Daniela SoledadPeláez, RafaelGarcía Sanmartín, JosuneNarro Íñiguez, JuditSoliño, ManuelFernández, Juan CarlosSarotto, Aníbal JoséCiranna, Nicolás S.López Costa, Juan JoséDorfman, Verónica BertaLarráyoz, Ignacio M.Loidl, C. FabiánMartínez, AlfredoAPOPTOSISCOLD-SHOCK PROTEINSELECTRORETINOGRAMHYPOTHERMIAPERINATAL ASPHYXIAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.Fil: Rey Funes, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Contartese, Daniela Soledad. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Peláez, Rafael. Centro de Investigación Biomédica de La Rioja; EspañaFil: García Sanmartín, Josune. Centro de Investigación Biomédica de La Rioja; EspañaFil: Narro Íñiguez, Judit. Centro de Investigación Biomédica de La Rioja; EspañaFil: Soliño, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Fernández, Juan Carlos. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Sarotto, Aníbal José. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Ciranna, Nicolás S.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: López Costa, Juan José. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Larráyoz, Ignacio M.. Centro de Investigación Biomédica de La Rioja; EspañaFil: Loidl, C. Fabián. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Martínez, Alfredo. Centro de Investigación Biomédica de La Rioja; EspañaFrontiers Media2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165332Rey Funes, Manuel; Contartese, Daniela Soledad; Peláez, Rafael; García Sanmartín, Josune; Narro Íñiguez, Judit; et al.; Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats; Frontiers Media; Frontiers in Pharmacology; 12; 4-2021; 1-131663-9812CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fphar.2021.651599/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fphar.2021.651599info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:40Zoai:ri.conicet.gov.ar:11336/165332instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:40.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
title Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
spellingShingle Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
Rey Funes, Manuel
APOPTOSIS
COLD-SHOCK PROTEINS
ELECTRORETINOGRAM
HYPOTHERMIA
PERINATAL ASPHYXIA
title_short Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
title_full Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
title_fullStr Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
title_full_unstemmed Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
title_sort Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats
dc.creator.none.fl_str_mv Rey Funes, Manuel
Contartese, Daniela Soledad
Peláez, Rafael
García Sanmartín, Josune
Narro Íñiguez, Judit
Soliño, Manuel
Fernández, Juan Carlos
Sarotto, Aníbal José
Ciranna, Nicolás S.
López Costa, Juan José
Dorfman, Verónica Berta
Larráyoz, Ignacio M.
Loidl, C. Fabián
Martínez, Alfredo
author Rey Funes, Manuel
author_facet Rey Funes, Manuel
Contartese, Daniela Soledad
Peláez, Rafael
García Sanmartín, Josune
Narro Íñiguez, Judit
Soliño, Manuel
Fernández, Juan Carlos
Sarotto, Aníbal José
Ciranna, Nicolás S.
López Costa, Juan José
Dorfman, Verónica Berta
Larráyoz, Ignacio M.
Loidl, C. Fabián
Martínez, Alfredo
author_role author
author2 Contartese, Daniela Soledad
Peláez, Rafael
García Sanmartín, Josune
Narro Íñiguez, Judit
Soliño, Manuel
Fernández, Juan Carlos
Sarotto, Aníbal José
Ciranna, Nicolás S.
López Costa, Juan José
Dorfman, Verónica Berta
Larráyoz, Ignacio M.
Loidl, C. Fabián
Martínez, Alfredo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
COLD-SHOCK PROTEINS
ELECTRORETINOGRAM
HYPOTHERMIA
PERINATAL ASPHYXIA
topic APOPTOSIS
COLD-SHOCK PROTEINS
ELECTRORETINOGRAM
HYPOTHERMIA
PERINATAL ASPHYXIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.
Fil: Rey Funes, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Contartese, Daniela Soledad. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Peláez, Rafael. Centro de Investigación Biomédica de La Rioja; España
Fil: García Sanmartín, Josune. Centro de Investigación Biomédica de La Rioja; España
Fil: Narro Íñiguez, Judit. Centro de Investigación Biomédica de La Rioja; España
Fil: Soliño, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Fernández, Juan Carlos. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Sarotto, Aníbal José. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Ciranna, Nicolás S.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: López Costa, Juan José. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Larráyoz, Ignacio M.. Centro de Investigación Biomédica de La Rioja; España
Fil: Loidl, C. Fabián. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Martínez, Alfredo. Centro de Investigación Biomédica de La Rioja; España
description Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/165332
Rey Funes, Manuel; Contartese, Daniela Soledad; Peláez, Rafael; García Sanmartín, Josune; Narro Íñiguez, Judit; et al.; Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats; Frontiers Media; Frontiers in Pharmacology; 12; 4-2021; 1-13
1663-9812
CONICET Digital
CONICET
url http://hdl.handle.net/11336/165332
identifier_str_mv Rey Funes, Manuel; Contartese, Daniela Soledad; Peláez, Rafael; García Sanmartín, Josune; Narro Íñiguez, Judit; et al.; Hypothermic shock applied after perinatal asphyxia prevents retinal damage in rats; Frontiers Media; Frontiers in Pharmacology; 12; 4-2021; 1-13
1663-9812
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3389/fphar.2021.651599
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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