Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-1...

Autores
Traglia, German Matias; Pasteran, Fernando; Escalante, Jenny; Nishimura, Brent; Tuttobene, Marisel Romina; Subils, Tomás; Nuñez, Maria Rosa; Rivollier, María Gabriela; Corso, Alejandra; Tolmasky, Marcelo E.; Ramirez, Maria Soledad
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: After the emergence of COVID-19, numerous cases of A. baumannii/SARSCoV-2 co-infection were reported. Whether the co-infecting A. baumannii strains have distinctivecharacteristics remains unknown. Methods and Results: A. baumannii AMA_NO was isolated in2021 from a patient with COVID-19. AMA166 was isolated from a mini-BAL used on a patient withpneumonia in 2016. Both genomes were similar, but they possessed 337 (AMA_NO) and 93 (AMA166) unique genes that were associated with biofilm formation, flagellar assembly, antibiotic resistance, secretion systems, and other functions. The antibiotic-resistance genes were found within mobile genetic elements. While both strains harbored the carbapenemase-coding gene blaOXA-23, only the strain AMA_NO carried blaNDM-1. Representative functions coded for by virulence genes are the synthesis of the outer core of lipooligosaccharide (OCL5), biosynthesis and export of the capsular polysaccharide (KL2 cluster), high-efficiency iron uptake systems (acinetobactin and baumannoferrin), adherence, and quorum sensing. A comparative phylogenetic analysis including 239 additional sequence type (ST) 2 representative genomes showed high similarity to A. baumannii ABBL141. Since the degree of similarity that was observed between A. baumannii AMA_NO and AMA166 is higher than that found among other ST2 strains, we propose that they derive from a unique background based on core-genome phylogeny and comparative genome analysis. Conclusions: Acquisition or shedding of specific genes could increase the ability of A. baumannii to infect patients with COVID-19.
Fil: Traglia, German Matias. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Nishimura, Brent. California State University; Estados Unidos
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Nuñez, Maria Rosa. Provincia del Neuquen. Hospital Provincial Neuquen "dr. E. Castro Rendon"; Argentina
Fil: Rivollier, María Gabriela. Hospital Doctor Artemides Zatti ; Gobierno de la Provincia de Rio Negro;
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
Materia
ACINETOBACTER BAUMANNII MDR
ST-2
COVID-19
BLA-OXA23
BLA-NDM1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240638
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 PandemicTraglia, German MatiasPasteran, FernandoEscalante, JennyNishimura, BrentTuttobene, Marisel RominaSubils, TomásNuñez, Maria RosaRivollier, María GabrielaCorso, AlejandraTolmasky, Marcelo E.Ramirez, Maria SoledadACINETOBACTER BAUMANNII MDRST-2COVID-19BLA-OXA23BLA-NDM1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: After the emergence of COVID-19, numerous cases of A. baumannii/SARSCoV-2 co-infection were reported. Whether the co-infecting A. baumannii strains have distinctivecharacteristics remains unknown. Methods and Results: A. baumannii AMA_NO was isolated in2021 from a patient with COVID-19. AMA166 was isolated from a mini-BAL used on a patient withpneumonia in 2016. Both genomes were similar, but they possessed 337 (AMA_NO) and 93 (AMA166) unique genes that were associated with biofilm formation, flagellar assembly, antibiotic resistance, secretion systems, and other functions. The antibiotic-resistance genes were found within mobile genetic elements. While both strains harbored the carbapenemase-coding gene blaOXA-23, only the strain AMA_NO carried blaNDM-1. Representative functions coded for by virulence genes are the synthesis of the outer core of lipooligosaccharide (OCL5), biosynthesis and export of the capsular polysaccharide (KL2 cluster), high-efficiency iron uptake systems (acinetobactin and baumannoferrin), adherence, and quorum sensing. A comparative phylogenetic analysis including 239 additional sequence type (ST) 2 representative genomes showed high similarity to A. baumannii ABBL141. Since the degree of similarity that was observed between A. baumannii AMA_NO and AMA166 is higher than that found among other ST2 strains, we propose that they derive from a unique background based on core-genome phylogeny and comparative genome analysis. Conclusions: Acquisition or shedding of specific genes could increase the ability of A. baumannii to infect patients with COVID-19.Fil: Traglia, German Matias. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Escalante, Jenny. California State University; Estados UnidosFil: Nishimura, Brent. California State University; Estados UnidosFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Nuñez, Maria Rosa. Provincia del Neuquen. Hospital Provincial Neuquen "dr. E. Castro Rendon"; ArgentinaFil: Rivollier, María Gabriela. Hospital Doctor Artemides Zatti ; Gobierno de la Provincia de Rio Negro;Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Ramirez, Maria Soledad. California State University; Estados UnidosMDPI2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240638Traglia, German Matias; Pasteran, Fernando; Escalante, Jenny; Nishimura, Brent; Tuttobene, Marisel Romina; et al.; Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic; MDPI; Biology; 12; 3; 2-2023; 1-142079-7737CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-7737/12/3/358info:eu-repo/semantics/altIdentifier/doi/10.3390/biology12030358info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:52Zoai:ri.conicet.gov.ar:11336/240638instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:52.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
title Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
spellingShingle Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
Traglia, German Matias
ACINETOBACTER BAUMANNII MDR
ST-2
COVID-19
BLA-OXA23
BLA-NDM1
title_short Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
title_full Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
title_fullStr Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
title_full_unstemmed Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
title_sort Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
dc.creator.none.fl_str_mv Traglia, German Matias
Pasteran, Fernando
Escalante, Jenny
Nishimura, Brent
Tuttobene, Marisel Romina
Subils, Tomás
Nuñez, Maria Rosa
Rivollier, María Gabriela
Corso, Alejandra
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author Traglia, German Matias
author_facet Traglia, German Matias
Pasteran, Fernando
Escalante, Jenny
Nishimura, Brent
Tuttobene, Marisel Romina
Subils, Tomás
Nuñez, Maria Rosa
Rivollier, María Gabriela
Corso, Alejandra
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author_role author
author2 Pasteran, Fernando
Escalante, Jenny
Nishimura, Brent
Tuttobene, Marisel Romina
Subils, Tomás
Nuñez, Maria Rosa
Rivollier, María Gabriela
Corso, Alejandra
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII MDR
ST-2
COVID-19
BLA-OXA23
BLA-NDM1
topic ACINETOBACTER BAUMANNII MDR
ST-2
COVID-19
BLA-OXA23
BLA-NDM1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: After the emergence of COVID-19, numerous cases of A. baumannii/SARSCoV-2 co-infection were reported. Whether the co-infecting A. baumannii strains have distinctivecharacteristics remains unknown. Methods and Results: A. baumannii AMA_NO was isolated in2021 from a patient with COVID-19. AMA166 was isolated from a mini-BAL used on a patient withpneumonia in 2016. Both genomes were similar, but they possessed 337 (AMA_NO) and 93 (AMA166) unique genes that were associated with biofilm formation, flagellar assembly, antibiotic resistance, secretion systems, and other functions. The antibiotic-resistance genes were found within mobile genetic elements. While both strains harbored the carbapenemase-coding gene blaOXA-23, only the strain AMA_NO carried blaNDM-1. Representative functions coded for by virulence genes are the synthesis of the outer core of lipooligosaccharide (OCL5), biosynthesis and export of the capsular polysaccharide (KL2 cluster), high-efficiency iron uptake systems (acinetobactin and baumannoferrin), adherence, and quorum sensing. A comparative phylogenetic analysis including 239 additional sequence type (ST) 2 representative genomes showed high similarity to A. baumannii ABBL141. Since the degree of similarity that was observed between A. baumannii AMA_NO and AMA166 is higher than that found among other ST2 strains, we propose that they derive from a unique background based on core-genome phylogeny and comparative genome analysis. Conclusions: Acquisition or shedding of specific genes could increase the ability of A. baumannii to infect patients with COVID-19.
Fil: Traglia, German Matias. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Nishimura, Brent. California State University; Estados Unidos
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Nuñez, Maria Rosa. Provincia del Neuquen. Hospital Provincial Neuquen "dr. E. Castro Rendon"; Argentina
Fil: Rivollier, María Gabriela. Hospital Doctor Artemides Zatti ; Gobierno de la Provincia de Rio Negro;
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
description Background: After the emergence of COVID-19, numerous cases of A. baumannii/SARSCoV-2 co-infection were reported. Whether the co-infecting A. baumannii strains have distinctivecharacteristics remains unknown. Methods and Results: A. baumannii AMA_NO was isolated in2021 from a patient with COVID-19. AMA166 was isolated from a mini-BAL used on a patient withpneumonia in 2016. Both genomes were similar, but they possessed 337 (AMA_NO) and 93 (AMA166) unique genes that were associated with biofilm formation, flagellar assembly, antibiotic resistance, secretion systems, and other functions. The antibiotic-resistance genes were found within mobile genetic elements. While both strains harbored the carbapenemase-coding gene blaOXA-23, only the strain AMA_NO carried blaNDM-1. Representative functions coded for by virulence genes are the synthesis of the outer core of lipooligosaccharide (OCL5), biosynthesis and export of the capsular polysaccharide (KL2 cluster), high-efficiency iron uptake systems (acinetobactin and baumannoferrin), adherence, and quorum sensing. A comparative phylogenetic analysis including 239 additional sequence type (ST) 2 representative genomes showed high similarity to A. baumannii ABBL141. Since the degree of similarity that was observed between A. baumannii AMA_NO and AMA166 is higher than that found among other ST2 strains, we propose that they derive from a unique background based on core-genome phylogeny and comparative genome analysis. Conclusions: Acquisition or shedding of specific genes could increase the ability of A. baumannii to infect patients with COVID-19.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240638
Traglia, German Matias; Pasteran, Fernando; Escalante, Jenny; Nishimura, Brent; Tuttobene, Marisel Romina; et al.; Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic; MDPI; Biology; 12; 3; 2-2023; 1-14
2079-7737
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240638
identifier_str_mv Traglia, German Matias; Pasteran, Fernando; Escalante, Jenny; Nishimura, Brent; Tuttobene, Marisel Romina; et al.; Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic; MDPI; Biology; 12; 3; 2-2023; 1-14
2079-7737
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3390/biology12030358
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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