Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain

Autores
Silva, Marta G.; Ueti, Massaro W.; Norimine, Junzo; Jacobsen, Monica Ofelia; Bastos, Reginaldo G.; Goff, Will L.; Brown, Wendy C.; Oliva, Abel; Suarez, Carlos E.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines. © 2010 Elsevier Ireland Ltd.
Fil: Silva, Marta G.. Universidade Nova de Lisboa; Portugal
Fil: Ueti, Massaro W.. Animal Disease Research Unit; Estados Unidos
Fil: Norimine, Junzo. Washington State University; Estados Unidos
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bastos, Reginaldo G.. Washington State University; Estados Unidos
Fil: Goff, Will L.. Animal Disease Research Unit; Estados Unidos
Fil: Brown, Wendy C.. Washington State University; Estados Unidos
Fil: Oliva, Abel. Universidade Nova de Lisboa; Portugal
Fil: Suarez, Carlos E.. Animal Disease Research Unit; Estados Unidos
Materia
Babesia Bovis
Bbo-Mic1
In Vitro Neutralization Assay
Mic Proteins
Micronemes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53570
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53570
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domainSilva, Marta G.Ueti, Massaro W.Norimine, JunzoJacobsen, Monica OfeliaBastos, Reginaldo G.Goff, Will L.Brown, Wendy C.Oliva, AbelSuarez, Carlos E.Babesia BovisBbo-Mic1In Vitro Neutralization AssayMic ProteinsMicronemeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines. © 2010 Elsevier Ireland Ltd.Fil: Silva, Marta G.. Universidade Nova de Lisboa; PortugalFil: Ueti, Massaro W.. Animal Disease Research Unit; Estados UnidosFil: Norimine, Junzo. Washington State University; Estados UnidosFil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bastos, Reginaldo G.. Washington State University; Estados UnidosFil: Goff, Will L.. Animal Disease Research Unit; Estados UnidosFil: Brown, Wendy C.. Washington State University; Estados UnidosFil: Oliva, Abel. Universidade Nova de Lisboa; PortugalFil: Suarez, Carlos E.. Animal Disease Research Unit; Estados UnidosElsevier Ireland2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53570Silva, Marta G.; Ueti, Massaro W.; Norimine, Junzo; Jacobsen, Monica Ofelia; Bastos, Reginaldo G.; et al.; Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain; Elsevier Ireland; Parasitology International; 59; 2; 6-2010; 294-2971383-5769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.parint.2010.03.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383576910000577info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:42Zoai:ri.conicet.gov.ar:11336/53570instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:43.096CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
title Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
spellingShingle Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
Silva, Marta G.
Babesia Bovis
Bbo-Mic1
In Vitro Neutralization Assay
Mic Proteins
Micronemes
title_short Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
title_full Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
title_fullStr Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
title_full_unstemmed Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
title_sort Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain
dc.creator.none.fl_str_mv Silva, Marta G.
Ueti, Massaro W.
Norimine, Junzo
Jacobsen, Monica Ofelia
Bastos, Reginaldo G.
Goff, Will L.
Brown, Wendy C.
Oliva, Abel
Suarez, Carlos E.
author Silva, Marta G.
author_facet Silva, Marta G.
Ueti, Massaro W.
Norimine, Junzo
Jacobsen, Monica Ofelia
Bastos, Reginaldo G.
Goff, Will L.
Brown, Wendy C.
Oliva, Abel
Suarez, Carlos E.
author_role author
author2 Ueti, Massaro W.
Norimine, Junzo
Jacobsen, Monica Ofelia
Bastos, Reginaldo G.
Goff, Will L.
Brown, Wendy C.
Oliva, Abel
Suarez, Carlos E.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia Bovis
Bbo-Mic1
In Vitro Neutralization Assay
Mic Proteins
Micronemes
topic Babesia Bovis
Bbo-Mic1
In Vitro Neutralization Assay
Mic Proteins
Micronemes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines. © 2010 Elsevier Ireland Ltd.
Fil: Silva, Marta G.. Universidade Nova de Lisboa; Portugal
Fil: Ueti, Massaro W.. Animal Disease Research Unit; Estados Unidos
Fil: Norimine, Junzo. Washington State University; Estados Unidos
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bastos, Reginaldo G.. Washington State University; Estados Unidos
Fil: Goff, Will L.. Animal Disease Research Unit; Estados Unidos
Fil: Brown, Wendy C.. Washington State University; Estados Unidos
Fil: Oliva, Abel. Universidade Nova de Lisboa; Portugal
Fil: Suarez, Carlos E.. Animal Disease Research Unit; Estados Unidos
description A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines. © 2010 Elsevier Ireland Ltd.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53570
Silva, Marta G.; Ueti, Massaro W.; Norimine, Junzo; Jacobsen, Monica Ofelia; Bastos, Reginaldo G.; et al.; Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain; Elsevier Ireland; Parasitology International; 59; 2; 6-2010; 294-297
1383-5769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53570
identifier_str_mv Silva, Marta G.; Ueti, Massaro W.; Norimine, Junzo; Jacobsen, Monica Ofelia; Bastos, Reginaldo G.; et al.; Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain; Elsevier Ireland; Parasitology International; 59; 2; 6-2010; 294-297
1383-5769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.parint.2010.03.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383576910000577
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981251396403200
score 12.48226

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