SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation

Autores
Cattoni, Diego Ignacio; Chara, Osvaldo; Godefroy, Cédric; Margeat, Emmanuel; Trigueros, Sonia; Milhiet, Pierre Emmanuel; Nöllmann, Marcelo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
SpoIIIE/FtsK are membrane‐anchored, ATP‐fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence‐recognition modules (SpoIIIE‐γ/FtsK‐γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single‐molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single‐molecule and modelling methods that hexameric SpoIIIE binds DNA non‐specifically and finds SRS by an ATP‐independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE‐γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS‐directed translocation by SpoIIIE/FtsK.
Fil: Cattoni, Diego Ignacio. Universite Montpellier II; Francia
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Universitat Dresden; Alemania
Fil: Godefroy, Cédric. Technische Universität Dresden; Francia
Fil: Margeat, Emmanuel. Universite Montpellier II; Francia
Fil: Trigueros, Sonia. University of Oxford; Reino Unido
Fil: Milhiet, Pierre Emmanuel. Universite Montpellier II; Francia
Fil: Nöllmann, Marcelo. Universite Montpellier II; Francia
Materia
Translocation Directionality
Sequence-Specific Regulation
Dna Segregation
Molecular Motors
Single Molecule
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23681
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23681
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulationCattoni, Diego IgnacioChara, OsvaldoGodefroy, CédricMargeat, EmmanuelTrigueros, SoniaMilhiet, Pierre EmmanuelNöllmann, MarceloTranslocation DirectionalitySequence-Specific RegulationDna SegregationMolecular MotorsSingle Moleculehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1SpoIIIE/FtsK are membrane‐anchored, ATP‐fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence‐recognition modules (SpoIIIE‐γ/FtsK‐γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single‐molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single‐molecule and modelling methods that hexameric SpoIIIE binds DNA non‐specifically and finds SRS by an ATP‐independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE‐γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS‐directed translocation by SpoIIIE/FtsK.Fil: Cattoni, Diego Ignacio. Universite Montpellier II; FranciaFil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Universitat Dresden; AlemaniaFil: Godefroy, Cédric. Technische Universität Dresden; FranciaFil: Margeat, Emmanuel. Universite Montpellier II; FranciaFil: Trigueros, Sonia. University of Oxford; Reino UnidoFil: Milhiet, Pierre Emmanuel. Universite Montpellier II; FranciaFil: Nöllmann, Marcelo. Universite Montpellier II; FranciaEmbo Press2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23681Cattoni, Diego Ignacio; Chara, Osvaldo; Godefroy, Cédric; Margeat, Emmanuel; Trigueros, Sonia; et al.; SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation; Embo Press; Embo Reports; 14; 5; 4-2013; 473-4791469-221X1469-3178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/embor.2013.39info:eu-repo/semantics/altIdentifier/url/http://embor.embopress.org/content/14/5/473info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:51Zoai:ri.conicet.gov.ar:11336/23681instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:51.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
title SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
spellingShingle SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
Cattoni, Diego Ignacio
Translocation Directionality
Sequence-Specific Regulation
Dna Segregation
Molecular Motors
Single Molecule
title_short SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
title_full SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
title_fullStr SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
title_full_unstemmed SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
title_sort SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
dc.creator.none.fl_str_mv Cattoni, Diego Ignacio
Chara, Osvaldo
Godefroy, Cédric
Margeat, Emmanuel
Trigueros, Sonia
Milhiet, Pierre Emmanuel
Nöllmann, Marcelo
author Cattoni, Diego Ignacio
author_facet Cattoni, Diego Ignacio
Chara, Osvaldo
Godefroy, Cédric
Margeat, Emmanuel
Trigueros, Sonia
Milhiet, Pierre Emmanuel
Nöllmann, Marcelo
author_role author
author2 Chara, Osvaldo
Godefroy, Cédric
Margeat, Emmanuel
Trigueros, Sonia
Milhiet, Pierre Emmanuel
Nöllmann, Marcelo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Translocation Directionality
Sequence-Specific Regulation
Dna Segregation
Molecular Motors
Single Molecule
topic Translocation Directionality
Sequence-Specific Regulation
Dna Segregation
Molecular Motors
Single Molecule
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv SpoIIIE/FtsK are membrane‐anchored, ATP‐fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence‐recognition modules (SpoIIIE‐γ/FtsK‐γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single‐molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single‐molecule and modelling methods that hexameric SpoIIIE binds DNA non‐specifically and finds SRS by an ATP‐independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE‐γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS‐directed translocation by SpoIIIE/FtsK.
Fil: Cattoni, Diego Ignacio. Universite Montpellier II; Francia
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Universitat Dresden; Alemania
Fil: Godefroy, Cédric. Technische Universität Dresden; Francia
Fil: Margeat, Emmanuel. Universite Montpellier II; Francia
Fil: Trigueros, Sonia. University of Oxford; Reino Unido
Fil: Milhiet, Pierre Emmanuel. Universite Montpellier II; Francia
Fil: Nöllmann, Marcelo. Universite Montpellier II; Francia
description SpoIIIE/FtsK are membrane‐anchored, ATP‐fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence‐recognition modules (SpoIIIE‐γ/FtsK‐γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single‐molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single‐molecule and modelling methods that hexameric SpoIIIE binds DNA non‐specifically and finds SRS by an ATP‐independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE‐γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS‐directed translocation by SpoIIIE/FtsK.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23681
Cattoni, Diego Ignacio; Chara, Osvaldo; Godefroy, Cédric; Margeat, Emmanuel; Trigueros, Sonia; et al.; SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation; Embo Press; Embo Reports; 14; 5; 4-2013; 473-479
1469-221X
1469-3178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23681
identifier_str_mv Cattoni, Diego Ignacio; Chara, Osvaldo; Godefroy, Cédric; Margeat, Emmanuel; Trigueros, Sonia; et al.; SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation; Embo Press; Embo Reports; 14; 5; 4-2013; 473-479
1469-221X
1469-3178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/embor.2013.39
info:eu-repo/semantics/altIdentifier/url/http://embor.embopress.org/content/14/5/473
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Embo Press
publisher.none.fl_str_mv Embo Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613943447781376
score 13.070432

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