Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells

Autores
Leiros, Gustavo Jose; Kusinsky, Ana Gabriela; Drago, Hugo; Bossi, Silvia; Sturla, Flavio; Castellanos, Maria Lia; Stella, Inés Yolanda; Balaña, Maria Eugenia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and may contribute to the understanding of skin regeneration. The growth of hair follicles in vitro or after grafting remains a major challenge. The dermal-epidermal composites are skin substitutes comprised of dermal fibroblasts (DF) embedded in a matrix overlaid with keratinocytes. Hair Follicle Stem Cells (HFSC) contribute to hair follicle regeneration and wound repair. Dermal papilla cells (DPC) signaling orchestrates hair follicle morphogenesis and regeneration. The use of DPC as dermal component in a permanent composite skin with human HFSC was evaluated by studying tissue-engineered skin architecture, stem cell persistence and hair regeneration as well as the graft-take in nude mice. A porcine acellular dermal matrix (ADM) was seeded with HFSC alone and with human DPC or DF. Histological and immunohistochemical analyses of in vitro constructs were performed. The presence of DPC induced a more regular and multi-layered stratified epidermis with more basal p63-positive cells and invaginations. Graft-take and tissue remodeling in nude mice were favored in DPC-containing composite skin supported by the fact of graft-epidermis survival and early neovascularization. Interestingly, only in grafted constructs containing DPC, embryonic hair bud-like structures were observed from 14 days after grafting. These structures showed cells of human origin, presence of precursor epithelial cells and expression of a hair differentiation marker. These observations suggest an incipient hair follicle neogenesis inside the remodeling ADM. Taken together our results show DPC and HFSC as promising cellular components for a permanent skin substitute.
Fil: Leiros, Gustavo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Kusinsky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Drago, Hugo. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; Argentina
Fil: Bossi, Silvia. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; Argentina
Fil: Sturla, Flavio. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; Argentina
Fil: Castellanos, Maria Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Stella, Inés Yolanda. Universidad Maimónides. Centro de Estudios Biomédicos, Ambientales y Diagnóstico; Argentina
Fil: Balaña, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Materia
Skin Substitutes
Dermal Papilla Cells
Hair Follicle Neogenesis
Hair Follicles Stem Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4110
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4110
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem CellsLeiros, Gustavo JoseKusinsky, Ana GabrielaDrago, HugoBossi, SilviaSturla, FlavioCastellanos, Maria LiaStella, Inés YolandaBalaña, Maria EugeniaSkin SubstitutesDermal Papilla CellsHair Follicle NeogenesisHair Follicles Stem Cellshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and may contribute to the understanding of skin regeneration. The growth of hair follicles in vitro or after grafting remains a major challenge. The dermal-epidermal composites are skin substitutes comprised of dermal fibroblasts (DF) embedded in a matrix overlaid with keratinocytes. Hair Follicle Stem Cells (HFSC) contribute to hair follicle regeneration and wound repair. Dermal papilla cells (DPC) signaling orchestrates hair follicle morphogenesis and regeneration. The use of DPC as dermal component in a permanent composite skin with human HFSC was evaluated by studying tissue-engineered skin architecture, stem cell persistence and hair regeneration as well as the graft-take in nude mice. A porcine acellular dermal matrix (ADM) was seeded with HFSC alone and with human DPC or DF. Histological and immunohistochemical analyses of in vitro constructs were performed. The presence of DPC induced a more regular and multi-layered stratified epidermis with more basal p63-positive cells and invaginations. Graft-take and tissue remodeling in nude mice were favored in DPC-containing composite skin supported by the fact of graft-epidermis survival and early neovascularization. Interestingly, only in grafted constructs containing DPC, embryonic hair bud-like structures were observed from 14 days after grafting. These structures showed cells of human origin, presence of precursor epithelial cells and expression of a hair differentiation marker. These observations suggest an incipient hair follicle neogenesis inside the remodeling ADM. Taken together our results show DPC and HFSC as promising cellular components for a permanent skin substitute.Fil: Leiros, Gustavo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Kusinsky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Drago, Hugo. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; ArgentinaFil: Bossi, Silvia. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; ArgentinaFil: Sturla, Flavio. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; ArgentinaFil: Castellanos, Maria Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Stella, Inés Yolanda. Universidad Maimónides. Centro de Estudios Biomédicos, Ambientales y Diagnóstico; ArgentinaFil: Balaña, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaAlphamed Press2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4110Leiros, Gustavo Jose; Kusinsky, Ana Gabriela; Drago, Hugo; Bossi, Silvia; Sturla, Flavio; et al.; Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells; Alphamed Press; Stem Cells Translational Medicine; 3; 10; 8-2014; 1209-12192157-6564enginfo:eu-repo/semantics/altIdentifier/issn/http://dx.doi.org/10.5966%2Fsctm.2013-0217info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/stcltm/article/3/10/1209/6388150info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:56Zoai:ri.conicet.gov.ar:11336/4110instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:56.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
title Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
spellingShingle Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
Leiros, Gustavo Jose
Skin Substitutes
Dermal Papilla Cells
Hair Follicle Neogenesis
Hair Follicles Stem Cells
title_short Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
title_full Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
title_fullStr Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
title_full_unstemmed Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
title_sort Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells
dc.creator.none.fl_str_mv Leiros, Gustavo Jose
Kusinsky, Ana Gabriela
Drago, Hugo
Bossi, Silvia
Sturla, Flavio
Castellanos, Maria Lia
Stella, Inés Yolanda
Balaña, Maria Eugenia
author Leiros, Gustavo Jose
author_facet Leiros, Gustavo Jose
Kusinsky, Ana Gabriela
Drago, Hugo
Bossi, Silvia
Sturla, Flavio
Castellanos, Maria Lia
Stella, Inés Yolanda
Balaña, Maria Eugenia
author_role author
author2 Kusinsky, Ana Gabriela
Drago, Hugo
Bossi, Silvia
Sturla, Flavio
Castellanos, Maria Lia
Stella, Inés Yolanda
Balaña, Maria Eugenia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Skin Substitutes
Dermal Papilla Cells
Hair Follicle Neogenesis
Hair Follicles Stem Cells
topic Skin Substitutes
Dermal Papilla Cells
Hair Follicle Neogenesis
Hair Follicles Stem Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and may contribute to the understanding of skin regeneration. The growth of hair follicles in vitro or after grafting remains a major challenge. The dermal-epidermal composites are skin substitutes comprised of dermal fibroblasts (DF) embedded in a matrix overlaid with keratinocytes. Hair Follicle Stem Cells (HFSC) contribute to hair follicle regeneration and wound repair. Dermal papilla cells (DPC) signaling orchestrates hair follicle morphogenesis and regeneration. The use of DPC as dermal component in a permanent composite skin with human HFSC was evaluated by studying tissue-engineered skin architecture, stem cell persistence and hair regeneration as well as the graft-take in nude mice. A porcine acellular dermal matrix (ADM) was seeded with HFSC alone and with human DPC or DF. Histological and immunohistochemical analyses of in vitro constructs were performed. The presence of DPC induced a more regular and multi-layered stratified epidermis with more basal p63-positive cells and invaginations. Graft-take and tissue remodeling in nude mice were favored in DPC-containing composite skin supported by the fact of graft-epidermis survival and early neovascularization. Interestingly, only in grafted constructs containing DPC, embryonic hair bud-like structures were observed from 14 days after grafting. These structures showed cells of human origin, presence of precursor epithelial cells and expression of a hair differentiation marker. These observations suggest an incipient hair follicle neogenesis inside the remodeling ADM. Taken together our results show DPC and HFSC as promising cellular components for a permanent skin substitute.
Fil: Leiros, Gustavo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Kusinsky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Drago, Hugo. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; Argentina
Fil: Bossi, Silvia. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; Argentina
Fil: Sturla, Flavio. Hospital de Quemados de la Ciudad de Buenos Aires. Banco de Tejidos; Argentina
Fil: Castellanos, Maria Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Stella, Inés Yolanda. Universidad Maimónides. Centro de Estudios Biomédicos, Ambientales y Diagnóstico; Argentina
Fil: Balaña, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
description Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and may contribute to the understanding of skin regeneration. The growth of hair follicles in vitro or after grafting remains a major challenge. The dermal-epidermal composites are skin substitutes comprised of dermal fibroblasts (DF) embedded in a matrix overlaid with keratinocytes. Hair Follicle Stem Cells (HFSC) contribute to hair follicle regeneration and wound repair. Dermal papilla cells (DPC) signaling orchestrates hair follicle morphogenesis and regeneration. The use of DPC as dermal component in a permanent composite skin with human HFSC was evaluated by studying tissue-engineered skin architecture, stem cell persistence and hair regeneration as well as the graft-take in nude mice. A porcine acellular dermal matrix (ADM) was seeded with HFSC alone and with human DPC or DF. Histological and immunohistochemical analyses of in vitro constructs were performed. The presence of DPC induced a more regular and multi-layered stratified epidermis with more basal p63-positive cells and invaginations. Graft-take and tissue remodeling in nude mice were favored in DPC-containing composite skin supported by the fact of graft-epidermis survival and early neovascularization. Interestingly, only in grafted constructs containing DPC, embryonic hair bud-like structures were observed from 14 days after grafting. These structures showed cells of human origin, presence of precursor epithelial cells and expression of a hair differentiation marker. These observations suggest an incipient hair follicle neogenesis inside the remodeling ADM. Taken together our results show DPC and HFSC as promising cellular components for a permanent skin substitute.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4110
Leiros, Gustavo Jose; Kusinsky, Ana Gabriela; Drago, Hugo; Bossi, Silvia; Sturla, Flavio; et al.; Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells; Alphamed Press; Stem Cells Translational Medicine; 3; 10; 8-2014; 1209-1219
2157-6564
url http://hdl.handle.net/11336/4110
identifier_str_mv Leiros, Gustavo Jose; Kusinsky, Ana Gabriela; Drago, Hugo; Bossi, Silvia; Sturla, Flavio; et al.; Dermal Papilla Cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using Hair Follicle Stem Cells; Alphamed Press; Stem Cells Translational Medicine; 3; 10; 8-2014; 1209-1219
2157-6564
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/http://dx.doi.org/10.5966%2Fsctm.2013-0217
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/stcltm/article/3/10/1209/6388150
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Alphamed Press
publisher.none.fl_str_mv Alphamed Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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